Molecular cloning of a human polypeptide related to yeast sds22, a regulator of protein phosphatase-1.
FEBS Lett
; 375(1-2): 75-8, 1995 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-7498485
ABSTRACT
sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucine-rich repeat structures of 22 residues. Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Proteínas Nucleares
/
Núcleo Celular
/
Fosfoproteínas Fosfatases
/
Proteínas de Ciclo Celular
/
Proteínas de Schizosaccharomyces pombe
/
Fígado
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article