Induction of anti-DNA antibodies in non autoimmune mice by immunization with a DNA-DNAase I complex.
Clin Exp Rheumatol
; 13(1): 7-10, 1995.
Article
em En
| MEDLINE
| ID: mdl-7774106
ABSTRACT
Recent studies suggest that anti-DNA antibodies may arise from the immune response to a complex of DNA and a DNA-binding protein. One of the protein targets frequently recognized by anti-DNA antibodies is the enzyme DNAase I. To investigate the possible role of DNAase I in the induction of anti-DNA antibodies, we immunized mice with a complex of DNA and DNAase I. Mammalian double strand DNA was crosslinked with DNAase I by ultraviolet light (UV) treatment and emulsified in complete Freund's adjuvant. BALB/c mice were immunized at the base of the tail with the DNA-DNAase complex, boosted after 2 weeks with the immunogen in incomplete adjuvant and bled one week after the boost. Control mice received UV treated DNA in adjuvant. In one-third of the mice immunized with the DNA-DNAase complex, IgG anti-DNA antibodies were detectable in serum; the antibodies reacted with single and double strand DNA. No anti-DNA response was elicited by immunization with DNA alone. These data show that immunization with a DNA-DNAase complex can induce anti-DNA antibodies in non-autoimmune mice strains and suggest that DNA-binding proteins may act as carriers in the immune response that leads to anti-DNA antibody production.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Anticorpos Antinucleares
/
Imunização
/
Desoxirribonuclease I
Limite:
Animals
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article