Circadian regulation of iodopsin gene expression in embryonic photoreceptors in retinal cell culture.
Neuron
; 10(4): 579-84, 1993 Apr.
Article
em En
| MEDLINE
| ID: mdl-8476610
ABSTRACT
A circadian clock regulates a number of diverse physiological functions in the vertebrate eye. In this study, we show that mRNA for the red-sensitive cone pigment, iodopsin, fluctuates with a circadian rhythm in chicken retina. Transcript levels increase in the late afternoon just prior to the time of cone disc shedding. Furthermore, iodopsin mRNA levels are regulated similarly by a circadian oscillator in primary cultures of dispersed embryonic chick retina. Nuclear run-on experiments show that the circadian regulation of iodopsin transcript abundance occurs at the level of gene transcription. Our results provide a demonstration of clock-regulated gene expression in a vertebrate preparation maintained in cell culture.
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Base de dados:
MEDLINE
Assunto principal:
Células Fotorreceptoras
/
Retina
/
Pigmentos da Retina
/
Expressão Gênica
/
Ritmo Circadiano
/
Opsinas de Bastonetes
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article