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Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases.
Bini, L; Magi, B; Marzocchi, B; Cellesi, C; Berti, B; Raggiaschi, R; Rossolini, A; Pallini, V.
Afiliação
  • Bini L; Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy.
Electrophoresis ; 17(3): 612-6, 1996 Mar.
Article em En | MEDLINE | ID: mdl-8740187
ABSTRACT
Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of alpha 1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As the the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.
Assuntos
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Base de dados: MEDLINE Assunto principal: Infecções Bacterianas / Viroses / Proteínas de Fase Aguda / Eletroforese em Gel Bidimensional Tipo de estudo: Prognostic_studies Limite: Child / Child, preschool / Humans Idioma: En Ano de publicação: 1996 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Infecções Bacterianas / Viroses / Proteínas de Fase Aguda / Eletroforese em Gel Bidimensional Tipo de estudo: Prognostic_studies Limite: Child / Child, preschool / Humans Idioma: En Ano de publicação: 1996 Tipo de documento: Article