Selected peptides targeted to the NMDA receptor channel protect neurons from excitotoxic death.
Nat Biotechnol
; 16(3): 286-91, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9528011
ABSTRACT
Excitotoxic neuronal death, associated with neurodegeneration and stroke, is triggered primarily by massive Ca2+ influx arising from overactivation of glutamate receptor channels of the N-methyl-D-aspartate (NMDA) subtype. To search for channel blockers, synthetic combinatorial libraries were assayed for block of agonist-evoked currents by the human NR1-NR2A NMDA receptor subunits expressed in amphibian oocytes. A set of arginine-rich hexapeptides selectively blocked the NMDA receptor channel with IC50 approximately 100 nM, a potency similar to clinically tolerated blockers such as memantine, and only marginally blocked on non-NMDA glutamate receptors. These peptides prevent neuronal cell death elicited by an excitotoxic insult on hippocampal cultures.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Receptores de N-Metil-D-Aspartato
/
Neurônios
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article