Distinct chromosomal alterations associated with TP53 status of LoVo cells under PALA selective pressure: a parallel with cytogenetic pathways of colorectal cancers.
Oncogene
; 18(3): 775-81, 1999 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-9989828
ABSTRACT
This study investigates the chromosomal alterations involved in the acquisition of PALA resistance of LoVo colorectal cancer cells homozygous for wild-type TP53 before and after transfection with a 143Ala-mutated TP53 gene. PALA resistance was always associated with an increased number of CAD gene copies, but gene amplification sensu stricto was rarely observed. Interestingly, distinct chromosome patterns were found in relation to the TP53 status of the cells. In parental LoVo cells, the CAD copy number was increased through gains of normal chromosome 2 whereas in transfectant clones, resistance mostly occurred through chromosome rearrangements. The relationship with the two different cytogenetic patterns described in colorectal tumors is discussed.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Fosfonoacéticos
/
Neoplasias Colorretais
/
Proteína Supressora de Tumor p53
/
Aberrações Cromossômicas
/
Ácido Aspártico
/
Inibidores Enzimáticos
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article