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[Aim] To gain the fusion protein purified GST-AEP.[Methods] The protein GST-AEP was expressed in E.Coli-DH5? as a fusion protein induced by IPTG.The protein was a kind of inclusion body.The purifying and refolding to inclusion body were optimized.The purity of GST-AEP was identified by 12% SDS-PAGE and thin-layer scanning analysis.The quantitation of the fusion protein GST-AEP was done with BCA Protein Assay.[Results] Purity of GST-AEP was higher than 90% and concentration was about 0.163?g/?l.[Conclusion] The fusion protein was highly purified and the method of fusion protein purification from the inclusion body was developed,which was the basis for further study on AEP.
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Objective To study the antagonistic effect of N-acetylcysteine(NAC)on the damage of hepatic mitochondria of rats induced by cadmium in vitro.Methods The mitochondria were prepared from the clean Wistar rats' whole liver by using differ ential centfifugation.The mitochondria were incubated in the assay buffer containing different concentration of CdCl_2 (10,100,1 000,10 000 ?mol/L)at 37 ℃ for 1 h.The effect of NAC(500 ?mol/L)was studied at a CdCl_2 concentration of 1 000 ?mol/L.The incubation buffer was collected and the level of GSH,cytochrome C and the activity of Mn-SOD were determined. Results Compared with the control group,the level of GSH and Mn-SOD in 100,1 000,10 000 ?mol/L CdCl_2 groups were significantly decreased,the content of cytochrome C in 1 000,10 000 ?mol/L CdCl_2 groups were significantly increased(P
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Objective To analyze the dermal irritation characteristics of 8 types of cosmetics.Methods The selected 917 cosmetics samples of 8 types,which were underwent the health safety test in China during 2005-2007,were assessed in the acute dermal irritation according to the related standards and the data were statistically analyzed using CMH method.Results The dermal-irritant samples were detected in different levels in 8 types of cosmetics.In terms of the irritation of cosmetics and the dermal damage caused by cosmetics,the cosmetics for hair,for face clean and for bath showed a significant higher proportion compared with the other types of cosmetics and the dermal damage could last for more than 14 days.Conclusion The acute dermal-irritability is different in 8 types of cosmetics,the cosmetics for hair,for face clean and for bath can cause the irritation and damage in the skin in degrees.
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Objective To investigate the effect of basic fibroblast growth factor(bFGF)on the apoptosis of scleral cells in the posterior pole in lens-induced myopia of guinea pigs and to discuss the mechanism of bFGF in inhibiting the formation of myopia. Methods Four-week-old cleaning healthy guinea pigs were randomly divided into the control group,LIM group,LIM+PBS group, LIM+the bFGF 100 ng group,LIM+the bFGF 500 ng group,LIM+the bFGF 1 000 ng group,15 in each.Except the control group, the right eye of guinea pig in other groups wore-10 D concave lens for 7 days and then different concentration of bFGF or PBS were injected into the vitreous cavity,3 days later injected again.After 15 days of-10 D concave lens treatment,the eyeballs were removed and the apoptotic cells in the scleras were determined by electron microscopy,TUNEL technique and flow cytometry.The proliferation of scleral cells in the posterior pole were determined by Ki-67 immune histochemistry stain.Results Compared with the control group,the significant differences were detected in the right eyes of LIM group(P
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Objective To understand the acute effect of ambient air CO pollution on daily mortality of cerebrocardiovascular diseases among highly and long-term exposed residents of 65 years and over in Taiyuan,Shanxi,China.Methods The relationship of CO concentration and daily mortality of cerebrocardiovascular diseases among residents over 65 years old in Taiyuan(2003-2004) was analyzed by case-crossover design and conditional Logistic regression in SAS 9.0 Results The 48 h accumulated CO concentration had the most significant effect.As increment of CO average was 100 ?g/m~3,the corresponding OR of the effect on the total deaths of the cerebrocardiovascular diseases,the cardiac disease,the ischemic heart disease,myocardial infarction,cardiac failure,arrhythmia and stroke was 1.006,1.010,1.007,1.005,1.005,1.006 and 1.012 respectively.Under different air pollution conditions,the pollutant presented different effects on deaths of eerebrocardiovascular diseases.Conclusion The current CO pollution has caused a certain adverse effect on the cerebrocardiovascular mortality among residents of 65 years and over in Taiyuan city.More stringent measures should be taken to control the air pollution and decrease CO level,thus reduce the mortality of cerebrocardiovascular diseases.
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Objective To know the relationship between the changes of total anti-oxidation capacity (T-AOC),nitric oxide (NO) and nitric oxide synthase (NOS) the liver and liver damage induced by chronic fluoride exposure.Methods Eighteen male Wistar rats were randomly divided into 3 groups,control(treated with tap water),low fluoride(LF,treated with NaF at 100 mg/L) and high fluoride (HF,treated with NaF at 200 mg/L),6 in each group.Fluoride was given through drinking water.During the period of exposure,the body was weighed and after 6 months of treatment,the rats were killed,the liver coefficient was calculated,T-AOC, NO and NOS in the livers were determined respectively.Results Compared with the control,the liver coefficient,NO and NOS in the fluoride treated groups significantly increased (P
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Objective To know the apolipoprotein E(APOE)genotypes frequency of Chinese old people in the rural area. Methods 2000 Chinese aged 65 years or older from four sites in China were enrolled in this study in 2004-2008.Two sites were from the Sichuan province in southwestern China,and another two sites were from the Shandong province in eastern China.The finger blood samples on filter paper were collected from all the investigated people in the end of the interview.The genotype for apolipoprotein E(gene symbol,APOE)was determined by eluting DNA from a dried blood spot,followed by HhaI digestion of amplified products.Results Through statistical analysis,APOE genotypes frequency of Chinese people aged 65 years or older in the rural area,as for ?/?2,?2/?3,?2/?4,?3/?3,?3/?4,?4/?4,they were 1.1%,13.55%,2.05%,68.80%,13.35% and 1.15% respectively.4 carriers and no 4 carriers were 16.55% and 83.45%.Conclusion The distribution of APOE 4 genotype is more widely in Chinese people aged 65 years or older in the rural area.
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Objective To explore the effects of benzirnidazole on spermatogenesis function and enzymatic activity in testis of male rats.Methods 40 male Wistar rats,clean degree,were randomly divided into four groups,10 in each.The rats in the low- dose group (20 mg/kg),the moderate-dose group (100 mg/kg) and the high-dose (200 mg/kg) were treated with benzimidazole at different doses by garage,once a day for 80 consecutive days.Simultaneously,the rats control group were treated with the equivalent volume of distilled water and tween-80.In the end of the experiment,the rats were weighed,the testis and epididymides were immediately excised and weighed,the appearance was observed,and the viscera coefficients of the bilateral testis and epididymides were calculated.The number and motility of sperms in the tail of epididymis,the activity of some enzymes (LDH, ACP,SDH,G-6-PDH) of testis were tested.Results The testis and epididymides in the control group and the low-dose group were pink,large,smooth and plump,but they were mauve,small,obviously atrophic in the moderate-dose group and the high-dose group. The viscera coefficient of the testis and epididymides,the number and motility of sperms in the tail of epididymis were significantly decreased in the moderate-dose group and the high-dose group (P
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Objective To investigate the health condition of swimmers who swim in a river in Guangzhou city and to know the influencing factors in order to provide the scientific data for making the diseases control guide for swimmers swimming in the river.Methods The swimmers who swam in a river in Guangzhou city were selected and the cluster sampling survey was conducted by telephone interview and questionnaires in 2006.The statistic analysis of the data was done with the chi-square and Logistic regression.Results 17.9% of the investigated swimmers felt disconffortable in the first time to swim in the river,the main symptoms were eye and skin symptoms,12.2% had the same symptoms in second time to swim in the river,there was a significant difference between the prevalence rates of the eye symptoms.The Logistic regression analysis showed that the following factors were associated with the prevalence of discomfortable symptoms:age,gender,swimming with any kinds of tampon in unclean river water,in the first time to swim in the river and in the second time to swim in the river,the related OR values were 0.95 and 0.97,3.55 and 2.32,14.83 and 6.7.Other factors associated with the prevalence of discomfortable symptoms were as follows:swimming period in unclean fiver water,swimming stroke,chokes the water,times of choking water,using earplugs,head position in water,the related OR values were 1.06,0.50,2.1,1.22,0.44,1.65.Concousion To swim in the natural unclean river may cause the discomfort,the main problems are eye and skin symptoms.
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Objective To study the activity of type 2 Iodothyronine deiodinase(D2)and the expressions of myelin basic protein(MBP)and synapsinⅠin the brain tissue of young rats fed on a diet with different levels of iodine.Methods Wistar rats were fed on a diet with different doses of KIO_3 for 3 months and then mated randomly.The serum TH and the brain D2 activity were measured in 28 days old pups.The protein expressions of MBP and SynapsinⅠin their brains were determined by immunohistochemistry staining.Results Compared with normal iodine group(NI),the serum TH levels of low iodine group(LI) were lower,while those of iodine excess groups were gradually decreased with their increase of iodine intake,especially in 100-fold high iodine group(100 HI),TT_4 and FT_4 were significantly decreased(P0.05).The immunohistochemistry staining showed weakly positive reactivity of MBP in corpus callosum and stronger of synapsin I in hippocampus CA3 in LI group compared with NI. The similar alterations were also found in all iodine excess groups with their increase of iodine intake.But MBP reactivity was stronger in 100 HI rats than the LI ones.Conclusion Iodine deficiency and iodine excess can cause hypothyroidism in degrees in the young rats,more severe hypothyroid and retarded myelin sheath and synapses can be caused in iodine deficiency compared with iodine excess.
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Objective To develop a high performance liquid chromatography (HPLC) for the determination of fourteen dyes in hair dying formulations.Methods Dyes were separated on a amide bonded C_(16) silica column (250 mm?4.6 mm,5?m),the target analytes were ion-paired prior to HPLC analysis with elution employing methanol-0.025 mol/L phosphate buffer (pH=6.0) containing 1 g/L 1-heptanesulfonic acid sodium salt as mobile phase and detection by a photodiode array detector with the detection wavelength of 230 nm and 280 nm.The flow rate was 1.0 ml/min and the column temperature was 25℃.Results The linear range were 10-500 mg/L,the detection limits were 0.3-2.0 mg/L,the coeffieients of variation were less than 10%(except 2-amino-6-chloro-4-nitrophenol at low concentration) and the recovery rates were 71.3%-118.5%.Conclusion The methods of high performance liquid chromatography introduced in this paper is simple,rapid,accurate and is applicable to the analysis of various hair dyes.
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Objective To know the microcystins pollution caused by cyanobactcria bloom in two drinking water sources in Ningbo city.Methods Water samples were collected at four sampling sites in Yaojiang River and three sampling sites in Miehu reservoir from 20 July,2007 to 31 August,2007.The algae in these water samples were classified and counted,and Microcystin-LR (MC-LR),MC-RR were determined by UPLC-MS-MS.Results The Cyanobacteria cell concentration in Yaojiang River and Meihu reservoir ranged from 0.8?10~6 to 6.7?10~7,and from 1.21?10~7 to 9.928?10~8 cells/L respectively.The MC-LR and MC-RR in Yaojiang River weren't detected,and neither was the MC-RR in MeiHu reservoir.However,the MC-LR was detected at sometimes,and the maximum concentration of MC-LR was 0.84?g/L.Conclusion There is no microcystins pollution in Yaojiang River,but there existed MC-LR pollution in MeiHu Reservoir,and the MC-LR concentration in it was below national standard limit of 1.0?g/L after the treatment of controlling alage and microsystins.
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Objective To study the adsorption performance of chitosan in printing and dyeing wastewater treatment.Methods The influence factors such as chitosan concentrations(0-500 mg/L),pH value(1-13),temperatures(20-50 ℃)and time(0.5-2.5 h) were considered in the test.Results When the concentration of chitosan was 200 mg/L,pH value was 2-5,time was 0.5 h and at the room temperature,the absorption could show a good result.The deeolorizing rate could reach above 90%.Conclusion The chitosan concentrations,pH value,time and temperature affect the adsorption performance of chitosan in printing and dyeing wastewater treatment.
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Objective To understand the effect of vanadium to liver ultramicro structure in SD rats.Methods One hundred and fifty generaMevel SD rats of 2 months were randomly divided into control groups,30 in each,the males and females were equivalent,treated with ammonium metavanadate at doses of 0,10,20,40,60 mg/L through drinking water.After 2,6 and 10 weeks of treatment,6 rats from each group were weighed and the changes of ultramicro-structure in the liver were examined with electron microscope.Results As the increasing of dose,the increase in body weight showed a downward trend,but the coefficient of liver showed a increasing trend,the consistent performance was seen in male and female rat.After two weeks of exposure,the rats acted,ate and drank less,hair roughing and no shining,often getting together were seen.After 10 weeks,the rats had not the basic activities,the hair was disheveled and loose,some died,the structure of mitochondria and endoplasmic reticulum was normal in the 10,20,40 mg/L group,the number of endoplasmic reticulum increased,in 60 mg/L group,the rough endoplasmic reticulum vanished,the liver cytoplasm had a swelling phenomenon,the mitochondria reduced.Conclusion Vanadium have little effect on the liver cells of rats in low exposure level,but high level exposure may damage the liver cells in rats.
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Objective To investigate the effects of electromagnetic irradiation on the activity of protein kinase C(PKC)in the cerebellum of rabbit.Methods Thirty rabbits were randomly divided into control and electromagnetic irradi.ation group (including 0 hour,3 hours,12 hours,24 hours and 72 hours subgroups after irradiation),rabbits in the electromagnetic irradiation group were exposed to 65 mW/cm~2 S frequency range electromagnetic irradiation for 30 minutes,the rectal temperature was taken immediately after irradiation and the specific absorption rate(SAR)values were calculated,the activity of PKC the cerebellum was determined with improved TaKai method.Results The rectal temperature of rabbits increased 2.35 ℃ after irradiation,SAR value was 4.54 kcal/kg.PKC activity decreased significantly after irradiation.Conclusion After 30 min of electromagnetic irradiation at 65 mW/cm~2,obvious heating effect will be detected in the rabbit and PKC activity in the cerebellum decreased significantly.
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Objective To study the bovine coronary artery endothelial cells(BCAEC)damage induced by cigarette abstracts and further clarify the relationship between smoking and cardiovascular diseases.Methods BCAEC were treated with nicotine, mainstream smoke extract(MSW)and sidestream smoke extract(SSW)which had the normal concentration(1.0?10~(-5),0.8?10~(-5), 0.9?10~(-5)mol/L)of nicotine in smoker.The morphological changes of BCAEC were recorded by microscope digital image system. The quantification of apoptotic BCAEC cells was performed by visualization of nuclei stained with 4,6'-diamidino-2-phenylindole and trypan blue exclusion assay was used to examine the percentage of necrotic BCAEC.The caspase activity assay was employed to discuss the mechanism of BCAEC apoptosis.Results BCAEC exposed to nicotine and MSW appeared the typical morphological alteration of apoptosis and necrotic morphological alteration were observed after BCAWC were treated with SSW. 5.89% and 11.94% apoptotic ceils were found after BCAEC were exposed to nicotine and MSW for 24 hours.The level of BCAEC necrosis after treated with SSW was 62.84%.Caspase-3 activity was induced by nicotine and MSW.Conclusion Cigarette smoke extract can induce the cell death of BCAEC.Nicotine and MSW can induce caspase-3 activity increase.Even in the presence of a non-cytotoxic concentration of nicotine and mainstream smoke solution,protease-induced apoptosis of BCAEC can be significantly increased.Sidestream smoke solution may cause BCAEC necrosis instead of apoptosis.Caspase-3 activation is probably the mechanism of BCAEC apoptosis.
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Objective To study the effects of nitrobenzene on macrophage function and lymphocyte proliferation in mice. Methods ICR mice were divided into groups and treated with nitrobenzene by gavage,once a day,at doses of 2,20 and 200 mg/kg respectively,for 21 consecutive days.The mice were killed after 21 days of treatment and then the effects of nitrobenzene on the organs index,the maerophage function and the lymphocyte proliferation were determined.Results The maerophage function and the lymphocyte proliferation decreased as the increase of the dose of nitrobenzene.Conclusion The results of the present paper show that nitrobenzene may inhibit the immunity function of mice.
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Phthalate esters belong to the group of environmental hormones,which are ubiquitous environmental pollutants and they can damage the human health through breathing to get into the body.The recent researches on the analysis of phthalate esters in the air such as sampling,pretreatment and determination were reviewed and some related issues were discussed in the paper.It would be reference for the further study of phthalate esters.
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Objective To investigate the organophosphorus pesticides residues in vegetables in Ji'nan,China.Methods From 2005 to 2007,the residues of ten kinds of organaphosphorous pesticides,including methamidophos,parathion-ethyl, parathionm-ethyl,dichlorvos,phorate,monocrotophos,omethoate,dimethoate,chlorpyrifos,malathion were determined in 8 kinds of vegetables by capillary gas chromatography.Results In the 597 vegetables samples,the total detection rate of organaphosphorous pesticides was 32.66%,in 25.63% of the samples,the contents of organaphosphorous pesticides residues exceeded the national standard limits.In leek,the detection rate was 65.56% and the exceeded limit rate was 52.22%.In addition, more than one kind of pesticide could be detected in one kind of vegetable.The concentration of methamidophos,the most serious pollutant,could reach 114.013 mg/kg.Omethoate could also be detected at 11.807 mg/kg.In this investigation,a downtrend was seen in the rate of exceeding standard limits and the difference in seasons in the detection rate of pesticides residues was also seen. Conclusion The detection rate of high toxic organaphosphorous pesticides in vegetables sampled in this study is higher,it indicates that the situation of pesticides abuse is serious in this area.
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0.05).Chlorpyrifos increased the activity of CYP1A1 in lungs,CYP2B1 in kidneys and lungs.Cypermethrin increased activity of CYP1A1 in livers,kidneys and lungs,CYP2B1 in livers and lungs,CYP3A in livers.These increases were statistically significant (P