الملخص
OBJECTIVE To study the impr ovement effects of Dianxianqing granule on blood-brain barrier (BBB)injury in Alzheimer’s disease (AD)model mice by regulating NLR family pyrin domain containing 3(NLRP3)inflammasome signaling pathway. METHODS Totally 125 mice were randomly divided into sham operation group (n=25)and modeling group (n=100) by body weight. AD model was induced by intracerebroventricular injection of β-amyloid 25-35 in model group. Sham operation group was given normal saline with same method. The 100 model mice were randomly divided into model group ,Donepezil hydrochloride tablets group (positive control 1,1.3 mg/kg,i.g.),MCC950 group [positive control 2(selective NLRP 3 inhibitor),10 mg/kg,i.p.] and Dianxianqing granule group (12.48 g/kg by crude drug ,i.g.)by body weight ,with 25 mice in each group. Second day after modeling ,administration groups were given relevant medicine ,once a day ,for consecutive 21 d. Sham operation group and model group were given intragastric administration of water and intraperitoneal injection of normal saline. At last administration,the learning and memory ability was determined by Y maze test ,and blood-brain barrier permeability was measured by Evans blue leakage assay. The expressions of NLRP 3,anti-ionized calcium-binding adapter molecule 1(IBA-1),nuclear factor kappa B (NF-κB)p65,p53 upregulated modulator of apoptosis (PUMA),occludin(ocln),zonula occluden- 1(ZO-1)and claudin-5 (cldn5) in cerebral tissue were determined. RESULTS Compared with model group , spontaneous alternate response rate ,protein expressions of ocln ,cldn5 lnzyxyqy2003@163.com and ZO- 1 in cerebral tissue were increased significantly in administration groups (P<0.05 or P<0.01);Evans blue E-mail:jiadg2003@126.com content and protein expressions of NLRP 3,IBA-1,PUMA and NF-κB p65 in cerebral tissue were decreased significantly (P<0.05 or P<0.01). CONCLUSIONS Dianxianqing granule can improve BBB injury of AD model m ice by inhibiting NLRP 3 inflammasome signaling pathway.
الملخص
BACKGROUND: The occurrenceand development of rheumatoid arthritis were strongly associated with unbalance proliferation and apoptosis of synovial cells and lymphocytes. Some synovial cell apoptosis was abnormal. OBJECTIVE: To observe effects of heterogenic double umbilical cord blood stem cell transplantation on Bcl-2 and Bax expression in mice with type Ⅱcollagen-induced arthritis. METHODS: C57BL/6(H-2b) mice were induced with Freund's complete adjuvant and type Ⅱcollagen to establish mouse models of type Ⅱcollagen-induced arthritis.At 2days after secondary immunity incubation, mice were injected with saline via tail vein in the model and normal control groups. Umbilical cord blood hemopoietic stem cells were injected into mouse tail vein in the UBSCs transplantation groups (single dose:2×106/50 g; double dose: 1×106/50 g each, totally 2×106/50 g). Mice were intragastrically administrated methotrexate in the methotrexate positive control group, 0.017 5 g/kg once, once every 5 days, totally six times. RESULTS AND CONCLUSION: Joint tissue below knee and elbow was obtained at42 days following transplantation. Histopathology displayed that smooth and glossy articular surface, no inflammatory cell infiltration in the synovial layer and normal chondrocytes in the normal control group. Hyperplasia, a lot of inflammatory cell infiltration and damaged cartilage surface were visible in the model group. Slight hyperplasia and a little inflammatory cell infiltration were detectable in the methotrexate positive controlgroup and single UBSCs transplantation group. Double UBSCs transplantation group exhibited smooth and glossy articular cartilage surface, no damage, a little inflammatory cell infiltration. Immunohistochemistry demonstrated that Bcl-2 and Bax expression was lower in the double UBSCs transplantation group compared with single UBSCs transplantation group (P 0.05). Results suggest that double umbilical cord blood stem cells can induce synovial cell apoptosis in mice with type Ⅱcollagen-induced arthritis in a certain number and action time, and protect synovial membrane against damage.