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BackgroundDiabetes distress is highly prevalent and has adverse impacts in adolescent patients with type 1 diabetes. However， the related factors of diabetes distress in adolescents with type 1 diabetes are not clear. ObjectiveTo identify the factors associated with diabetes distress in adolescents with type 1 diabetes using Meta-analysis， and to provide a scientific evidence for effective prevention and improvement of diabetes distress in adolescents with type 1 diabetes. MethodsOn December 1， 2022， a computerized search was conducted on databases including PubMed， Cochrane Library， Web of Science， Embase， CNKI， Wanfang Data， VIP and China Biomedical Literature Database， and studies relevant to diabetes distress in adolescents with type 1 diabetes were systematically included. Quality assessment of cross-sectional and cohort studies was carried out using criteria defined by the Agency for Healthcare Research and Quality （AHRQ） and Newcastle-Ottawa Scale （NOS）. Then the included studies were pooled in a Meta-analysis using Revman 5.3. ResultsA total of 22 studies were included， involving 6 442 adolescents with type 1 diabetes. Meta-analysis denoted that the occurrence of diabetes distress among adolescents with type 1 diabetes was correlated with age （r=0.094，95% CI： 0.042~0.145）， HbA1c （r=0.291， 95% CI： 0.248~0.335）， trait anxiety （r=0.585， 95% CI： 0.526~0.639）， depressive symptoms （r=0.635， 95% CI： 0.590~0.676）， resilience （r=-0.410， 95% CI： -0.528~-0.276） and parents' diabetes distress （r=0.462， 95% CI： 0.421~0.501）. ConclusionFactors including age， HbA1c， trait anxiety， depressive symptoms， resilience and parents' diabetes distress are correlated with diabetes distress in adolescents with type 1 diabetes. ［Funded by Sichuan Science and Technology Program （number， 24KJPX0034）］
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Abstract@#Social withdrawal is a kind of behavioral inhibition in social situations, which may increase the risk for maladjustment, internalizing and externalizing problems, interfering with psychological development and healthy growth. With the deepening understanding in sociology of development, child social withdrawal has gradually received extensive attention from scholars across the world. Understanding the phenomenon of child social withdrawal is important for in depth follow up research. Based on the literature review, the paper aims to summarize the types, mechanisms and influencing factors of social withdrawal in children, in order to provide scientific basis for formulating prevention strategies and early intervention programs in the future.
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Whole-room calorimeter is one of the gold standards for measuring energy expenditure, which is of great significance in studies on energy metabolism. It can be used to evaluate energy metabolism of different diets, physical activities and lifestyle in healthy or disease states as well as be applied into intervention plan development and efficacy evaluation of nutrition treatment in metabolic diseases, especially in obesity and anti-obesity treatment. It can also play a role in exploring the mechanism of metabolic disease when combined with metabolism related genes and molecular pathways. Here we aim to review the researches on whole-room calorimeter in energy expenditure measurement and obesity.
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Objective:To evaluate the clinical effect of Limb dysfunction after the comprehensive treatment of ischemic stroke by Traditional Chinese Medicine (TCM).Methods:A total of 160 patients with limb dysfunction after ischemic stroke. Who in line with the inclusion criteria, were randomly divided into 2 groups by random number table, 80 cases each. These patients were treated in Multicenter Union Hospital from June 2017 to Janunry 2019. The patients in the control group were given basic Western medicine treatment and rehabilitation training, while the patients in the observation group were given a comprehensive treatment combined with traditional Chinese medicine based on the control group (Chinese herbs, herbal fumigation and ear point press). Both groups were treated for 4 weeks and follow-up for 2 months. The Fugl-Meyerscale were used to evaluate the degree of limb dysfunction and balance dysfunction, the National Institutes of Health Stroke Scale were used for assessing the damage extent of nerve function, the Barthel index for evaluating the mobility of daily life. The clinical efficacy was evaluated according to the method of Brunnstrom assessment.Results:After treatment, the Limb strong spasm (1.57 ± 0.36 vs. 1.98 ± 0.53, t=5.724), Hemianesthesia (1.37 ± 0.31 vs. 1.80 ± 0.36, t=8.096), Inhibited bending and stretching (1.31 ± 0.25 vs. 1.84 ± 0.46, t=9.055) in the observation group were significantly lower than those in the control group ( P<0.01). The Fugl-Meyer activity and balance rating in the observation group were significantly higher than those in the control group ( t values were 2.739, 4.705, respectively, P<0.05 or P<0.01). The total effective rate of observation group was 93.3% (78/80), the control group was 73.3% (71/80), and there exist statistical significance ( χ2=4.783, P=0.028) in the two group’s comparative difference. Conclusion:The comprehensive TCM treatment can improve the hemiplegia syndrome of ischemic stroke patients and their limb activity and balance function, promote their neural functional recovery, enhance the activity of their daily life and clinical efficacy.
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ObjectiveTo understand the status of stigma and subjective well-being of patients with mental disorders and co-resident family members， to analyze the correlation between them， and to compare the impact of stigma upon the subjective well-being of patients and family members of the same household. MethodsPatients with mental disorders （n=154） and their families （n=154） who visited the outpatient department of a tertiary psychiatric hospital in Chengdu from October to November 2019 were selected， and four instruments were used for collecting the data， including self-designed demographic information questionnaire， self-designed disease-related information questionnaire， brief version of Internalized Stigma of Mental Illness Scale （ISMI-10） and Index of Well-Being Scale （IWB）. ResultsA total of 118 （76.62%） patients with mental disorders and 151 （98.05%） family members experienced stigma. The total score and each factors score in ISMI-10 of family members were higher than those of patients （P<0.01）， and the total score and each dimension score in IWB were lower than those of patients （P<0.01）. Pearson correlation analysis showed that ISMI-10 total score of patients and family members was negatively correlated with IWB total score （r=-0.600，-0.202， P<0.05 or 0.01）. After controlling demographic and disease-related variables， multiple linear regression analysis showed that the regression model of mental disorder patients achieved good fit effect （adjusted R2=0.457）， with statistical significance （F=26.746， P<0.01）， while the regression model of co-living family members was lack of fit （adjusted R2=0.035）. After controlling for family demographic variables， the model was at the statistical level （F=3.769， P<0.01）. ConclusionMental disorder patients have low illness stigma and high subjective well-being， whereas the opposite is true for family members. Moreover， there is a negative relationship between stigma and subjective well-being for both patients and family members， stigma is a significant factor affecting subjective well-being for people with mental disorders.
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OBJECTIVES@#To explore the effect of resveratrol (Res) on Kawasaki disease (KD)-induced myocardial injury and to evaluate its effect on apoptosis and autophagy.@*METHODS@#Forty-eight juvenile male Sprague Dawley rats were randomly divided into a control group, a Res group, a lactobacillus casei cell wall extract (LCWE)-induced Kawasaki disease group (KD group), and a LCWE-induced Kawasaki disease + Res treatment group (Res+KD group). The control group was intraperitoneally injected with saline. The Res group was intraperitoneally injected with resveratrol (100 mg/kg). The KD group was intraperitoneally injected with 0.5 mL LCWE (1 mg/mL). The Res+KD group was intraperitoneally injected with 0.5 mL LCWE (1 mg/mL) and resveratrol (100 mg/kg). After 4 weeks, the left ventricular ejection fraction (LVEF) and short axis shortening rate (LVFS) were detected by echocardiography. The apoptotic rate was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining. The levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), microtubule-associated protein 1 light chain 3β (LC3B), Beclin-1, autophagy related 5 (Atg5) and sequestosome-1 (p62) were detected by Western blotting. The formation of autophagosome was observed under transmission electron microscope.@*RESULTS@#There was no significant difference in the above-mentioned indexes between the control group and the Res group (all @*CONCLUSIONS@#Res can attenuate the KD-induced myocardial injury via inhibiting the apoptosis and autophagy.
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Animals , Male , Rats , Apoptosis , Autophagy , Mucocutaneous Lymph Node Syndrome/drug therapy , Rats, Sprague-Dawley , Resveratrol/therapeutic use , Stroke Volume , Ventricular Function, Leftالملخص
BACKGROUND: Recent studies have shown that Taohong Siwu Decoction can alleviate the damage of vascular endothelial cells and maintain their normal secretory function, and endothelial progenitor cells can promote the repair of endothelial injury. Therefore, it is assumed that Taohong Siwu Decoction may protect endothelial function by improving the functional activity and increasing the number of endothelial progenitor cells. OBJECTIVE: To investigate whether Taohong Siwu Decoction can augment the number and functional activity of peripheral blood endothelial progenitor cells. METHODS: Endothelial progenitor cells were isolated from the peripheral blood of healthy subjects, and divided into control, low-, moderateand high-concentration Taohong Siwu Decoction groups. Cells were then cultured to observe the dose-effect relationship within 24 hours. Meanwhile, the high-concentration Taohong Siwu Decoction group was cultured for respective time points (6, 12, 24 and 48 hours) for observing the time-effect relationship. The number of endothelial progenitor cells was counted under inverted phase contrast microscope. Proliferation, adhesion and migration of endothelial progenitor cells were detected by MTT chromatometry, adhesion activity assay and modified Boyden chamber assay, respectively. RESULTS AND CONCLUSION: (1) The proliferation, adhesion and migration abilities of endothelial progenitor cells in the Taohong Siwu Decoction groups were significantly higher than those in the control group and showed a certain dose-effect relationship. (2) The proliferation, adhesion and migration abilities of endothelial progenitor cells in the Taohong Siwu Decoction groups were enhanced in a time-dependent manner, especially at 24 hours after intervention (P < 0.01). To conclude, the Taohong Siwu Decoction can increase the number of endothelial progenitor cells and promote cell functions. High-concentration Taohong Siwu Decoction exhibits the best interventional effect at 24 hours after intervention.
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BACKGROUND: Recent studies have shown that Taohong Siwu Decoction can alleviate the damage of vascular endothelial cells and maintain their normal secretory function, and endothelial progenitor cells can promote the repair of endothelial injury. Therefore, it is assumed that Taohong Siwu Decoction may protect endothelial function by improving the functional activity and increasing the number of endothelial progenitor cells. OBJECTIVE: To investigate whether Taohong Siwu Decoction can augment the number and functional activity of peripheral blood endothelial progenitor cells. METHODS: Endothelial progenitor cells were isolated from the peripheral blood of healthy subjects, and divided into control, low-, moderateand high-concentration Taohong Siwu Decoction groups. Cells were then cultured to observe the dose-effect relationship within 24 hours. Meanwhile, the high-concentration Taohong Siwu Decoction group was cultured for respective time points (6, 12, 24 and 48 hours) for observing the time-effect relationship. The number of endothelial progenitor cells was counted under inverted phase contrast microscope. Proliferation, adhesion and migration of endothelial progenitor cells were detected by MTT chromatometry, adhesion activity assay and modified Boyden chamber assay, respectively. RESULTS AND CONCLUSION: (1) The proliferation, adhesion and migration abilities of endothelial progenitor cells in the Taohong Siwu Decoction groups were significantly higher than those in the control group and showed a certain dose-effect relationship. (2) The proliferation, adhesion and migration abilities of endothelial progenitor cells in the Taohong Siwu Decoction groups were enhanced in a time-dependent manner, especially at 24 hours after intervention (P < 0.01). To conclude, the Taohong Siwu Decoction can increase the number of endothelial progenitor cells and promote cell functions. High-concentration Taohong Siwu Decoction exhibits the best interventional effect at 24 hours after intervention.
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Objective To explore the effect of uric acid in cerebral small vessel disease and its cognitive impairment.Methods 102 patients with cerebral small vessel disease(CSVD)and 56 patients with NCSVD (non-CSVD) were enrolled.The CSVD imaging manifestations was assessed by brain MRI.Neuropsychological test was used to evaluate the cognitive function and serum uric acid concentration was detected.The uric acid levels was compared in CSVD and NCSVD groups and Logistic regression was used to assess the risk factor of CSVD.In patients with CSVD,the cognitive function was compared between the patients with and without hyperuricemia and partial correlation analysis was used to assess the relationship between uric acid and cognitive function.Results The serum uric acid level of patients with CSVD was higher than that of patients with NCSVD ((360.31 ±78.31) μmol /L,(301.79±49.37) μmol/L,P＜0.05).Uric acid was risk factor for patients with CSVD (OR=1.014,95％CI=1.007-1.021,P＜0.05).In patients with CSVD,there were significant differences in MMSE,VFT,DSST and TMT-A scores between patients with and without hyperuricemia (MMSE (23.76± 3.09) vs (22.27 ± 3.87);VIT (34.29 ± 8.01) vs (31.85 ± 6.16);DSST (34.39 ± 7.84) vs (31.15±7.47);TMT-A(59.10±17.52) vs (65.98±15.97),all P＜0.05).Uric acid was negatively correlated with MoCA,DST and VFT scores (r=-0.178,P=0.039;r=-0.202,P=0.018;r=-0.250,P=0.004),and positively correlated with TMT-B time cost (r=0.241,P=0.005).Conclusion Uric acid is a risk factor for CSVD and may damages the overall cognitive function,attention,language and executive function of patients with CSVD.
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Objective To evaluate the therapeutic effect of endovenous laser ablation (ELA) combined with foam sclerotherapy (FS) and mucopolysaccharide polysulfate (MP)cream on stasis dermatitis.Methods From December 2015 to May 2017,52 patients with 60 lesional limbs were enrolled from Department of Dermatology of Sichuan Provincial People's Hospital.The 60 lesional limbs were randomly and equally divided into 3 groups by a random number table and remainder grouping:combination group,MP group and control group.All the 3 groups were firstly treated with ELA in the main great saphenous vein.Then,the combination group was treated with FS followed by topical MP cream for 4 weeks.After the laser therapy,the MP group was treated with topical MP cream for 4 weeks,and the control group was treated with topical mometasone furoate cream alone for 4 weeks.The eczema area and severity index (EASI) and visual analogue scale (VAS) for itching scores in the above 3 group were recorded before treatment and 4 weeks after treatment.Statistical analysis was done by paired t-test for comparisons before and after treatment,one-way analysis of variance (ANOVA) for intergroup comparison,and least significant difference (LSD)-t test for multiple comparisons.Results No significant difference was observed before treatment among the combination group,MP group and control group in the EASI (9.64 ± 4.58,9.94 ± 4.18,9.50 ± 4.41 respectively,F =0.052,P ＞ 0.05) or VAS scores (7.25 ± 1.29,7.50 ± 1.19,7.45 ± 1.32 respectively,F =0.218,P ＞ 0.05).After 4-week treatment,the combination group,MP group and control group all showed significantly decreased EASI (3.54 ± 1.57,5.86 ± 2.39,7.04 ± 2.75 respectively) and VAS scores (2.35 ± 0.67,3.85 ± 0.67,4.65 ± 1.23 respectively) compared with those before treatment (t =4.30-18.80,all P ＜ 0.05).After 4-week treatment,the EASI score was significantly lower in the combination group than in the MP group and control group (both P ＜ 0.05),while there was no significant difference between the MP group and control group (P ＞ 0.05).Additionally,the VAS score was significantly lower in the combination group than in the MP group and control group (both P ＜ 0.05),as well as in the MP group than in the control group (P ＜ 0.05).Conclusions ELA combined with FS and topical MP cream shows better short-term efficacy for the treatment of stasis dermatitis compared with ELA combined with topical MP cream or mometasone furoate cream.The combination with topical MP cream is superior to that with topical mometasone furoate cream in improving itching.The long-term efficacy needs to be observed further.
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Objective To investigate therapeutic effects and complications of three kinds of minimally invasive therapies for varicosis of the lower extremities.Methods Totally,79 patients with 94 affected limbs were enrolled into this study.According to their clinical manifestations,3 kinds of minimally invasive therapies alone or in combination were selected,including endovenous laser treatment (EVLT) with saphenofemoral ligation and stripping of the great saphenous vein,microphlebectomy,foam sclerotherapy,EVLT with saphenofemoral ligation and stripping of the great saphenous vein + microphle-bectomy,and EVLT with saphenofemoral ligation and stripping of the great saphenous vein + foam sclerotherapy.Physical examination and color Doppler ultrasonography were performed to evaluate the regression and recurrence of varicosis,as well as complications.Meanwhile,dermatology life quality index (DLQI) was used to evaluate the improvement of life quality of the patients after the treatment.Results All the patients were followed up for 1-6 months (average,4.2 months),and no recurrence was observed.One month after the treatment,all the patients were re-evaluated.Of the 94 affected limbs,46 (48.9％) were cured,43 (45.7％) were improved,and 5 (5.3％) were unimproved.Three months after the treatment,69 patients with 82 limbs completed the re-examination.Of the 82 limbs,71 (86.6％)were cured,9 (11.0％) were improved,and 2 (2.4％) were unimproved.Six months after the treatment,61 patients with 70 limbs completed the follow-up.Of the 70 limbs,62 (88.6％) were cured,7 (10.0％) were improved,1 (1.4％) was unimproved.Repeated-measures analysis of variance revealed that the total DLQI scores before the treatment (9.12 ± 2.87),one month after the treatment (6.97 ± 2.39),3 months after the treatment (5.12 ± 1.96) and 6 months after the treatment (3.69 ± 1.45) significantly differed (F =328.84,P ＜ 0.01),and there were significantly differences between any two time points of re-evaluations (all P ＜ 0.01).In the 79 patients,postoperative complications included subcutaneous ecchymosis (7 patients,8.9％),numb sensation in the foot and boot area of the legs (6 patients,7.6％),and cord-like subcutaneous induration =(3 patients,3.8％).Conclusions The three kinds of minimally invasive therapies alone or in combination are all effective for the treatment of varicosis of the lower extremities with rapid recovery.The life quality of patients was obviously improved after the treatment,and the complications were acceptable.
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Objective To investigate the expression and its potential effect on the immune function of indoleamine 2 ,3-dioxygenase (IDO) in cutaneous malignant melanoma (MM). Methods The IDO protein expression in MM tissue from 30 patients,normal skin from 10 of these patients and normal foreskin of 10 healthy subjects were observed by skin immunohistochemical staining. The number of IDO positive cells were counted. Epidermal cells of MM tissue were isolated and cultured with Hanks solution contained 100 μmol/L tryptophan. The concentration of tryptophan metabolite and kynurenine were detected in supernatant ,reflecting the ability to metabolize the tryptophan of the skin cells. Further ,the isolated epidermal cells were co-cultured with T cells. The influence on PHA-stimulated T cell proliferation were observed. Results IDO(+)cells were almost undetectable in the normal skin,but IDO(+)cells greatly accumulated in epidermis of MM tissue. The percentage of IDO(+) cells/total cells[(28.5 ± 9.4)%]was significantly higher than that of normal skin from healthy subjects[(3.4 ± 1.5)%,P<0.05]and control skin from MM patient[(4.7 ± 2.3)%,P<0.05]. Compared with the epidermal cells from the healthy skin,the IDO(+)cells from MM tissue had a stronger ability to metabolize tryptophan into kyn-urenine in vitro. These isolated IDO-competent epidermal cellscould inhibit PHA-stimulated T cell proliferation. Conclusions IDO(+)cells greatly accumulated in cutaneous MM tissue ,which might induce a local immune tolerant microenvironment.
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Objective To investigate the expression and its potential effect on the immune function of indoleamine 2 ,3-dioxygenase (IDO) in cutaneous malignant melanoma (MM). Methods The IDO protein expression in MM tissue from 30 patients,normal skin from 10 of these patients and normal foreskin of 10 healthy subjects were observed by skin immunohistochemical staining. The number of IDO positive cells were counted. Epidermal cells of MM tissue were isolated and cultured with Hanks solution contained 100 μmol/L tryptophan. The concentration of tryptophan metabolite and kynurenine were detected in supernatant ,reflecting the ability to metabolize the tryptophan of the skin cells. Further ,the isolated epidermal cells were co-cultured with T cells. The influence on PHA-stimulated T cell proliferation were observed. Results IDO(+)cells were almost undetectable in the normal skin,but IDO(+)cells greatly accumulated in epidermis of MM tissue. The percentage of IDO(+) cells/total cells[(28.5 ± 9.4)%]was significantly higher than that of normal skin from healthy subjects[(3.4 ± 1.5)%,P<0.05]and control skin from MM patient[(4.7 ± 2.3)%,P<0.05]. Compared with the epidermal cells from the healthy skin,the IDO(+)cells from MM tissue had a stronger ability to metabolize tryptophan into kyn-urenine in vitro. These isolated IDO-competent epidermal cellscould inhibit PHA-stimulated T cell proliferation. Conclusions IDO(+)cells greatly accumulated in cutaneous MM tissue ,which might induce a local immune tolerant microenvironment.
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Aim To investigate the effects of midazolam on porcine isolated coronary artery rings pre-contracted by potassium chloride(KCl)and the possible mechanism.Methods The vessel tension recorder system was used.Isotonic tension of porcine isolated coronary artery rings precontracted by KCl(30 mmol·L-1)was recorded.The vasorelaxing action of midazolam and effects of various drugs were observed in the rings.Results Midazolam(3×10-6～1×10-4 mol·L-1)respectively concentration-dependently reduced the contraction induced by KCl,and there was significant difference between the rings with intact and denude endothelium(P<0.05).On KCl-induced precontraction,midazolam′s relaxation was depressed by L-NAME and the blend of L-NAME and L-Arg(P<0.05),but was not affected by Indo,L-Arg and 1400W.The contraction was not prevented by pretreatment with the inhibitor of Na+/Ca2+ exchanger(KB-R7943).The inhibitor of KATP(Gli)restrained the diastolic function of midazolam(P<0.05),while the inhibitor of BKCa(TEA),Kir(BaCl2),KV(4-AP)had no obvious effect.Conclusions Midazolam produces remarkable vasodilatation on KCl pre-contracted porcine isolated coronary artery rings.Its relaxtion effect is via concentration-dependent and endothelium-dependent mechanisms and relevant to the production of NO.Na+/Ca2+ exchanger is not involved midazolam′s vasodilatation on KCl pre-contracted porcine coronary artery rings.The relaxant mechanism of midazolam may be concerned with KATP.The Kir,BKCa and KV may be not involved.
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OBJECTIVE:To evaluate the methodological bias of systematic review and the reliability of counclusions of tradi-tional Chinese medicine (TCM) combined with transarterial chemoembolization in the treatment of primary liver cancer,and pro-vide evidence-based reference. METHODS:Retrieved from Cochrane Library,PubMed,Guideline,CBM,CJFD and Wanfang Da-tabase,high quality systematic review about TCM combined with transarterial chemoembolization in the treatment of primary liver cancer was collected for reevaluation. RESULTS:Totally 12 systematic review were involved. Results showed TCM combined with transarterial chemoembolization can improve tumor’s objective response,prolong the survival time of patients,improve life quali-ty,reduce postoperative adverse reactions with no serious adverse reactions reported related to TCM in the treatment of primary liv-er cancer. CONCLUSIONS:The clinical efficacy of TCM combined with transarterial chemoembolization is superior to transarterial chemoembolization alone in the treatment of primary liver cancer,with better safety.
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OBJECTIVE: To assess the feasibility of susceptibility-weighted imaging (SWI) while monitoring changes in renal oxygenation level after water loading. MATERIALS AND METHODS: Thirty-two volunteers (age, 28.0 +/- 2.2 years) were enrolled in this study. SWI and multi-echo gradient echo sequence-based T2* mapping were used to cover the kidney before and after water loading. Cortical and medullary parameters were measured using small regions of interest, and their relative changes due to water loading were calculated based on baseline and post-water loading data. An intraclass correlation coefficient analysis was used to assess inter-observer reliability of each parameter. A receiver operating characteristic curve analysis was conducted to compare the performance of the two methods for detecting renal oxygenation changes due to water loading. RESULTS: Both medullary phase and medullary T2* values increased after water loading (p 0.05). Interobserver reliability was excellent for the T2* values, good for SWI cortical phase values, and moderate for the SWI medullary phase values. The area under receiver operating characteristic curve of the SWI medullary phase values was 0.85 and was not different from the medullary T2* value (0.84). CONCLUSION: Susceptibility-weighted imaging enabled monitoring changes in the oxygenation level in the medulla after water loading, and may allow comparable feasibility to detect renal oxygenation level changes due to water loading compared with that of T2* mapping.
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Adult , Female , Humans , Male , Young Adult , Kidney/metabolism , Magnetic Resonance Imaging/methods , Oxygen/blood , ROC Curve , Reproducibility of Results , Water/administration & dosageالملخص
Objective To investigate the expression of gene Fmr1 in rats cortex, hippocampus and hypothalamus areas after the rapid eyes movement ( REM ) sleep deprivation .Methods Using the modified multiple platform method (MMPM), 126 rats were randomly and averagely divided into three groups , the normal control group ( CC), the environmental control group (TC) and the sleep deprivation group (SD).Each group was detected on day 1, day 2, day 3, day 5, day 7, and day 9, and the sample tissues were extracted from 7 rats at each time point.Immunohistochemistry and RT-PCR were operated to analysis the expression of gene Fmr 1.Results The expressions of gene Fmr1 were increased gradually in the cortex and thalamus of the SD group after 3 days ( P 0.05).The expressions of gene Fmr1 were decreased gradually in hippocampus for SD after 3 days ( P 0.05 ) . Conclusion The expressions of gene Fmr 1 were increased gradually in the cortex and thalamus but decreased in the hippocampus in the SD group after 3 days.
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of DE-MRI and the specificity and accuracy of LDDSE were significantly lower than that of DE-MRI (P < 0. 05). Conclusion DE-MRI with high specificity and accuracy, potentially, is a valuable imaging method for the detection of viable myocardium.
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Objective To investigate the effect of ginsenoside-Rgl on paraurethral fascia fibroblastsmultiplication and the expression of proliferation cell nuclear antigen (PCNA) of stress urinary incontinence(SUI) women in vitro. Methods Specimens of human paraurethral fascia were obtained from 4 SUI womenduring tension-free vaginal tape (TVT) or tension-free vaginal tape-obturator (TVT-O) procedure.Fibroblasts were isolated and cultured by outgrowth technique. After reaching confluency fibroblasts weresubcultured every 5 days and cells after passage number 3 to 5 were used for assessment. The paraurethralfascia fibroblasts were treated with ginsenoside-Rgl at different concentrations (5, 10, 20 μmol/L) andfibroblnsts without Rgl were used as controL The multiplication conditions of paraurethral fascia fibroblastswere respectively detected by methyl thiazolyl tetrazolium (MTr) assay and the expression of PCNA byhistochemistry. Results ( 1 ) Compared with the control group, the growth rate of cells treated with differentconcentrations of Rgl after 72h [ (29±5 )%, (40±5 )%, (26±4)% respectively ] was significantly higher(P<0.01). (2)Compared with the control group, the stimulatory effect of Rgl on fibroblast growth wassignificant at 24 h (P<0.01), and peaked at 72 hi (29±5)% ,(40±5)%, (26±4)% respectively, P<0.01]. (3)Compared with the control group(28.77% ), there was a significant increase of PCNA-positivecells (P<0.01) after 48 h treatment with different concentrations of Rgl (49.24%, 83.48%, 54.50%respectively). Conclusion The results indicate that, at least in vitro, fibroblasts from paraurethral fasciataken from women suffering from SUI are able to proliferate after
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AIMTo elucidate possible mechanisms underlying the differences between 1S-[1a,2b,3b,4a(S*)]-4-[7-[[1-(3-chloro-2-thienyl)methylpropyl]propyl-amino]-3H-imidazo[4,5-b]pyridyl-3-yl]-N-ethyl-2,3-dihydroxycyclopentane carboxamide (AMP- 579) and adenosine in pharmacological and clinical effects. METHODSNa+/Ca2+ exchange current was recorded by patch-clamp technique in whole-cell configuration. RESULTSAMP579 significantly enhanced both outward and inward Na+/Ca2+ exchange currents in a concentration dependent manner. Neither infusion of an adenosine A1 receptor antagonist PD116948 30 μmol*L-1 or an adenosine A2 receptor antagonist DMPX 10 μmol*L-1 nor a protein kinase A special blocker KT 5720 0.2 μmol*L-1 or a protein kinase C special blocker GF 109203X 0.4 μmol*L-1 had effect on Na+/Ca2+exchange current increased by AMP579, suggesting that AMP579 possess a direct activating effect on Na+/Ca2+ exchange current. CONCLUSIONAMP579 possibly possesses a direct activating effect on Na+/Ca2+ exchange current.