Effect of salvia miltiorrhiza and Ligustrazine injection on the early myocardial damage of patients with severe burn / 中华烧伤杂志

<p><b>OBJECTIVE</b>To observe the effect of salvia miltiorrhiza and ligustrazine injection on the early myocardial damage of severely burned patients.</p><p><b>METHODS</b>Twenty severely burned patients hospitalized from January 2010 to August 2011, with burn area equal to or more than 50% TBSA, were divided into two groups following hospitalization sequence, with odd number patients entering treatment group (T, n = 10) and even number patients entering control group (C, n = 10). Patients in C group were treated with routine methods, including fluid resuscitation based on the Third Military Medical University formula, anti-infection treatment, support treatment, and organ-protection treatment, etc. In addition to routine treatment methods, patients in T group received intravenous infusion of 250 mL glucose injection (50 g/L) containing 10 mL salvia miltiorrhiza and ligustrazine concoction, once a day, and continued for three days. Venous blood of patients was drawn at post burn hour (PBH) 12, 24, 48, and 72 to determine the plasma levels of cardiac troponin I (cTnI), creatine kinase isozyme MB (CK-MB), and atrial natriuretic peptide (ANP). Data were processed with t test.</p><p><b>RESULTS</b>At each time point, levels of cTnI, CK-MB, and ANP were lower in T group than in C group. Differences in contents of these parameters between two groups were statistically significant at most time points, with t values from 2.136 to 2.918, P < 0.05 or P < 0.01. Plasma levels of cTnI, CK-MB, and ANP in both groups peaked at PBH 12, which were respectively (28 ± 10) ng/mL, (76 ± 13) U/L, (430 ± 87) pg/mL in T group, and (38 ± 11) ng/mL, (87 ± 10) U/L, (453 ± 91) pg/mL in C group. From PBH 24 to 72, contents of above-mentioned parameters decreased gradually in both groups.</p><p><b>CONCLUSIONS</b>Early use of salvia miltiorrhiza and ligustrazine injection in severely burned patients can effectively reduce myocardial damage, thus protect the myocardium from injury.</p>

An experimental study on the in vivo intermingled culture of rat autologous and allogeneic epithelial cells / 中华烧伤杂志

<p><b>OBJECTIVE</b>To explore a method for in vivo skin reconstruction.</p><p><b>METHODS</b>Thirty Sprague-Dawley (SD) rats were randomly divided into two paired groups, i.e. in vivo culture (A group, 10 pairs) and in vitro grafting (B group, 5 pairs). Skin samples were harvested from the rats of the two groups for the isolation of epithelial cells which were then mixed cultured in vitro in 1:1 ratio. Mixed cellular suspension in A group was harvested 4 days after culture. The mixed cellular sheets were harvested 14 days after culture. The cultured cells and sheets were then transplanted onto total skin loss wounds of donor rats for further cultivation. The wounds in A group were covered with allogeneic full-thickness skin. While the wounds in B group were covered by collagen membrane and gauze. Wound repair was observed and compared between the two groups at 2 - 3 post-operative weeks.</p><p><b>RESULTS</b>Most of the wounds in A group healed after 2 - 3 weeks with smooth surface, and the peithelium connected closely and tightly with the subcutaneous tissue. In the wounds in B group on 5 post-operative day, some of the cellular sheets survived and some fell off. Even the healed wounds in B group would be injured again resulting in protracted small wounds.</p><p><b>CONCLUSION</b>In vivo in situ epithelial culture might be an optional method of skin reconstruction for wound healing.</p>