الملخص
A insuficiência renal aguda foi avaliada em 351 cadelas com piometra, selecionando-se 132 pelos critérios: creatinina > 2,4 mg/dL e/ou ureia > 80 mg/dL e/ou aumentos relativos de 100% de creatinina e/ou ureia séricas, no diagnóstico, 24 horas após a ovariossalpingohisterectomia e na retirada dos pontos. Foi administrado Ringer lactato de sódio até 90 mL/kg/h, durante quatro horas, ou três dias. Os parâmetros avaliados antes e após cada terapia foram: peso, pressão arterial sistólica (PAS), frequência cardíaca, função renal, hemogasimetria arterial, bioquímica sérica e urinária, unidade de remoção de ureia, fração de excreção de eletrólitos e clearance de creatinina. A análise univariada detectou diferença significante (p < 0,05) para: PAS, pH urinário, peso corpóreo, ureia e creatinina séricas, fração de excreção de eletrólitos e clearance da creatinina. Comparadas as sobreviventes (79,6%) com as não sobreviventes (20,4%), respectivamente, detectou-se creatinina sérica de 2,38 ± 1,33 mg/dL (média ± desvio padrão) e mediana 2,2 mg/dL e de 5,54 ± 3,0 mg/dL (média ± desvio padrão) e mediana 4,4 mg/dl, com diferença significante (p < 0,0001). Quanto ao clearance da creatinina endógena nas sobreviventes, 1,82 ± 1,74 mL/kg/min (média ± desvio padrão) e mediana 1,34 mg/dL e as não sobreviventes 0,36 ± 0,38 mg/dL (média ± desvio padrão) e mediana 0,23 mg/dL, com diferença significativa (p < 0,0001). Conclui-se que o critério de inclusão pode auxiliar na seleção dos animais em estado grave e o clearance da creatinina, considerado relevante quando observados valores > 1 mL/kg/min; negativo com valores < 1 mL/kg/min e indicador de falência renal grave e ou morte para valores < 0,5 ml/kg/min.
Acute renal failure (ARF) was evaluated prospectively in 351 female dogs with piometra, 132 animals were selected for this study, fulfilling the following criteria, measured in three moments (immediately after the diagnosis, 24 hours after the hysterectomy and when the suture was removed): creatinine > 2.4 mg/dL and/or urea > 80 mg/dL and/or relative increases of 100% in comparison with values previously obtained. The animals selected for the prospective study received conservative treatment consisting in lactated Ringer solution, with the maximum dose of 90 mL/kg/hr, during four hours. The factors evaluated before and after the therapy were: weight, systolic arterial pressure, heart rate, renal function, arterial hemogasometry, serum and urinary biochemistry and the endogenous renal creatinine clearance. The evaluated parameters showed significant statistical differences (p < 0.05) when companing: PAS (systolic arterial pressure), urinary pH, wheigt, serum urea and creatinine, excretion electrolyte rate and creatinine clearance. Howerer, the survining animals (79.6%) that received the treatment described above showed decresead levels of serum creatinine- 2,38 ± 1,33mg/dL and median 2.2 mg/dL when compared to the non-survining animals (20.4%) that received the same treatment 5.54 ± 3.0mg/dL and median 4.4mg/dL (p < 0.0001). When comparing the endogenous creatinine clearance, the survining animals had higher values 1.82 ± 1.74mL/kg/min and median 1.34 mL/kg/min than the non-survining animals 0.36 ± 0.38 mL/kg/min and median 0.23 mL/kg/min (p < 0.0001). Concluding: the criteria used to include the animals in this study can help to select the severely affected animals. Values higher than 1mL/kg/min are considered as a good prognosis while values lower than 1mL/kg/min are considered as a restricted prognosis. Values lower than 0.5 mL/kg/min are considered as strong predictors of severe renal failure or death.
الموضوعات
Acute Kidney Injury , Fluid Therapy , Uterus/pathology , Hysterectomy , Perioperative Careالملخص
As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6 percent) and 120 strains (79.5 percent) were positive for sfa, 86 (56.9 percent) were positive for cnf, 87 (57.6 percent) were positive for pap, 52 (34.4 percent) were positive for hly, 51 (33.8 percent) were positive for iuc and 5 (3.3 percent) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42 percent of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39-56 percent. Virulence factors were identified in 98.0 percent of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man.
A piometra é uma enfermidade da cadela adulta, sendo a doença reconhecida como uma das causas mais comuns de morte desta espécie animal. Os objetivos deste trabalho foram a avaliação de aspectos microbiológicos e histopatológicos da piometra canina e pesquisa de fatores de virulência de E. coli, identificando possíveis riscos para a saúde humana. Foi realizado o exame microbiológico de conteúdo intra-uterino de 100 cadelas com piometra bem como a contagem de unidades formadoras de colônias de microrganismos nestas amostras, testes de susceptibilidade "in vitro" aos antimicrobianos de E. coli e pesquisa de fatores de virulência nestas estirpes, e exame histopatológico de amostras de útero. Escherichia coli foi o microrganismo mais freqüentemente isolado (76,6 por cento), sendo que 120 estirpes (79,5 por cento) foram positivas para os genes sfa, 86 (56,9 por cento) foram positivas para cnf, 87 (57,6 por cento) foram positivas para pap, 52 (34,4 por cento) foram positivas para hly, 51 (33,8 por cento) foram positivas para iuc e 5 (3,3 por cento) foram positivas para afa. Observou-se maior sensibilidade de E.coli à norfloxacina, polimixina B, sulfazotrim, cloranfenicol e enrofloxacina. Em 42 por cento das amostras de parede uterina nas quais foram isolados microrganismos, os tamanhos das áreas de processo inflamatório corresponderam a 39-56 por cento. Foram identificados fatores de virulência em 98,0 por cento das estirpes de Escherichia coli avaliadas, demonstrando uma alta freqüência de E. coli potencialmente patogênica. Deve-se considerar que os cães são animais que vivem em proximidade aos homens há milhares de anos e possuem um papel importante na transmissão de E. coli aos outros animais e também ao homem.
الموضوعات
Animals , Dogs , Disease , Disease Susceptibility , Escherichia coli/isolation & purification , Histology , In Vitro Techniques , Microbiology , Methods , Methods , Virulenceالملخص
The clinical, gross and microscopical features of a granulosa cell tumour in the ovary of a 2-year-old cat are described. This is a rare tumour in cats, moreover because of the animal's age. It was diagnosed clinically by ultrasound examination. The tumour, a cystic mass in the region of the left ovary, metastasized to the epiplon and lungs. Tumour cells expressed vimentin and were arranged in a diffuse sarcomatous pattern, supported by a fine fibrovascular stroma. The presence of hair loss and repeated oestrus as clinical signs are indicative of orthoendocrine paraneoplastic syndrome, due to excessive oestrogen stimulation. This tumour should be considered in the differential diagnosis of behavioural disturbances in female cats