الملخص
Objective@#To discuss the effect of high-density fat-binding SVF-GEL in female facial lipofilling.@*Methods@#This is a retrospective study including 32 female patients, received facial fat transplantation during June 2017 to June 2018 in Yichun College. Each patient underwent high-density fat-binding SVF-GEL transplantation for facial surgery. Patients′satisfaction with the surgery and the rate of secondary surgery was evaluated. Fat was harvested from the inner thigh, centrifuged at 1200 g for 3 min, and the liquid was removed. The upper 2/3 part is prepared for SVF-GEL, for further used in delicate lipofilling in eyelid, tear groove and nasolabial groove. The lower 1/3 high density fat was used for volume restoration, such as forehead, temporal area and cheek.@*Results@#All patients had significant improvements in facial contours with mild swelling and short recovery time. The satisfaction rate was 68.8%(22/32), and the second operation rate was 15.6%(5/32).@*Conclusions@#High-density fat-binding SVF-GEL transplantation can achieve good results in correcting facial volume loss.
الملخص
Objective To investigate the expression of miR-124 in glioblastoma (GBM) cell lines LN229 and LN229R,as well as the regulatory mechanism of miR-124 on radiosensitivity of LN229R cells.Methods miR-124 mimic (miR-124) and negative control (miR-NC),STAT3 overexpression plasmid (STAT3) and pcDNA3.1 vector (pcDNA) were transfected or co-transfected into radioresistant glioma cells LN229R.qRT-PCR was employed to analyze the expression of miR-124 in LN229 and LN229R cells.The survival rate and sensitivity-related parameters of LN229R cells at different doses were analyzed by cloning formation assay.Cell apoptosis of LN229R was evaluated by flow cytometry.Targeting gene of miR-124 was predicted using Targetscan software and verified by the double-luciferase reporter assay.Western blot assay was performed to detect STAT3 protein expression.Results The expression of miR-124 in LN229R cells (0.32 ± 0.03) was significantly lower than that in LN229 cells (1.02 ± 0.09) (t =12.780,P＜0.05).Transfection of miR-124 mimics promoted the expression of miR-124 in LN229R cells (4.02±0.39) compared with miR-NC group (0.95±0.06) (t=13.476,P＜0.05).After 8 Gy irradiation,the survival rate of LN229R cells transfected with miR-124 mimics (0.003 ± 0.000 4) was significantly lower than that in miR-NC group (0.033±0.005 0) (t=5.655,P＜0.05),and the apoptosis rate (22.34±2.42) ％ was significantly higher than that in miR-NC group (4.69 ± 0.51) ％ (t =12.361,P＜0.05).STAT3 was identified to be a target gene of miR-124.Exogenous restoration of STAT3 reversed the inhibitory effect of miR-124 on LN229R cell survival.Conclusion miR-124 increases the radiosensitivity of LN229R cells by targeting STAT3.
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Objective To compare the effectiveness of stereotactic hematoma aspiration and conservative treatment for supratentorial hypertensive intracerebral hemorrhage (HICH) with hematoma volume 25-40 ml. Methods Patients with supratentorial HICH admitted to the Department of Neurosurgery, the Fifth Affiliated Hospital of Zhengzhou University from January 2014 to January 2017 were retrospectively enrolled. The incidence of rebleeding, good outcome (defined as the modified Rankin Scale score 0-2 at 3 months after onset) rate, and mortality were compared between the stereotactic hematoma aspiration group and the conservative treatment group. Results A total of 204 patients were enrolled. Their mean age was 61. 3 ±9. 2 years, 114 were males, and their median hematoma volume was 32 ml (interquartile range 25- 39 ml), median baseline Glasgow Coma Scale score was 11 (interquartile range 9-14), and there was no patient with brain herniation. One hundred and twenty patients (58. 8％) underwent stereotactic hematoma aspiration and 84 (41. 2％) received conservative treatment. Compared with the conservative treatment group, the incidence of rebleeding in the stereotactic hematoma aspiration group was significantly lower (2. 5％ vs. 22. 6％, χ2 =20. 788, P < 0. 001), and the rate of good outcome was significantly higher at 3 months after onset (85. 0％ vs. 70. 2％; χ2 = 8. 305, P = 0. 004 ), but there was no significant difference in mortality (5. 0％ vs. 11. 9％, χ2 =3. 259, P =0. 071). Multivariable logistic regression analysis showed that advanced age (odds ratio [OR] 1. 77, 95％ confidence interval [CI] 1. 25-2. 46; P = 0. 006), previous stroke history (OR 1. 36, 95％ CI 1. 12-1. 64; P =0. 032), and conservative treatment (OR 1. 42, 95％ CI 1. 25-1. 78; P = 0. 021) were the independent risk factors for poor outcomes. Conclusions Stereotactic hematoma aspiration can significantly reduce the incidences of rebleeding and risk of the poor outcome in the supratentorial HICH patients with hematoma volume 25-40 ml. Therefore, early active surgical treatment should be considered.
الملخص
Objective To explore the down-regulation of advanced receptor for advanced glycation end products(RAGE)on expression of high mobility group protein B1(HMGB1)in glioma cells line and the volume change of transplanted tumor in nude mice. Methods HMGB1 expression in glioma LN229 cells line (divided into a control group and a study group) was observed by immunohistochemical assay and Western blot. The control group received normal saline,whereas the study group received RAGE receptor blocking agent FPS-ZM1. Expression of HMGB1 protein was detected by the same methods. The difference of the expression was examined by independent sample t test. 30 Nu/Nu nude mice were randomly divided into two groups;the above two kinds cell lines were injected into the same area of the left back of nude mice. Six weeks after injection ,the volume size was measured six times ,and the variance of repeated measurement data was used to analyze the difference of the volume change. Results HMGB1 protein was mainly expressed in the cytoplasm and nucleus. As compared with the control group,HMGB1 protein expression levels were decreased in the study group(P < 0.05),the growth rate of transplanted tumor in nude mice was significantly faster in the control group than in the study group ,the difference was statistically significant(P < 0.05). Conclusions The growth and invasion of HMGB1 protein may be involved in glioma by RAGE receptor. RAGE receptor blocker FPS-ZM1 can significantly reduce the expression of HMGB1 protein and inhibit the growth of transplanted tumor volume. It is expected to be used for the research on glioma cell apoptosis.
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Objective To investigate the expression of high mobility group protein b 1 (HMGPB1) and toll-like receptors 4 (TLR4) in human glioma tissues of patients with epilepsy and explore the mechanism of glioma-associated epilepsy.Methods Ninety-five glioma specimens,collected during the resection surgery in our hospital firom June 2011 to June 2014,were used in our experiment;among them,55 were low-grade gliomas and 40 were high-grade gliomas.Immunohistochemistry and real time-PCR were used to assay the protein and mRNA expressions of HMGB1 and TLR4 in glioma tissues and peritumoral tissues,and the relation between HMGB1/TLR4 and glioma-associated epilepsy was analyzed.Results The incidence of glioma-associated epilepsy was 35.79％ (34/95),and the incidence of glioma-associated epilepsy in low-grade gliomas (49.09％) was significantly higher than that in high-grade gliomas (17.50％)(x2=10.057,P=0.002).Immunohistochemisty and RT-PCR showed that the protein and mRNA expressions of HMGB1/TLR4 in the peritumoral tissues with glioma-associated epilepsy were significantly higher than those in the peritumoral tissues without glioma-associated epilepsy (P＜0.05),while no significant difference was noted between glioma tissues with and without epilepsy (P＞0.05).Conclusion Over-expression of HMGB1 and TLR4 in peritumoral tissues of glioma patients may be related with the development of glioma-associated epilepsy,which may be a new target in glioma therapy.
الملخص
Objective To investigate the expressions ofnucleostemin (NS) and p53 up-regulated apoptotic factor (PUMA) proteins in human gliomas and its clinical significance.Methods Sixty-two human gliomas,collected in our hospital from January 2011 to February 2013 and confirmed by pathology,were used in our study; according to the WHO grading,grade Ⅰ included 8 patients in the study group,grade Ⅱ 21 patients,grade Ⅲ 19 patients and grade Ⅳ 14 patients.Other 10 patients accepted decompressions in our hospital at the same time period were used as control group.The expressions of NS and PUMA proteins in the brain tissues were detected by immunohistochemical S-P method.Results The NS protein expression was significantly higher and PUMA protein expression was statistically lower in study group than those in the control group (P＜0.05).As compared with gliomas of grade Ⅰ and Ⅱ,gliomas of grade Ⅲ and Ⅳ had significantly higher NS protein expression and lower PUMA protein expression (P＜0.05).Conclusions The expression changes of NS and PUMA are closely associated with gliomas malignant degrees,and the two play important roles in the progression and proliferation of glioma cells.