الملخص
Objective To investigate the effect of comprehensive nursing intervention on pregnancy outcome in patients with artificial insemination by husband. Methods 80 patients with artificial insemination by husband in the Department of Reproductive Medicine of Yichun People's Hospital from July 2016 to July 2018 were collected and divided into two groups by random number table method. The control group was treated with routine care intervention. The observation group was treated with comprehensive nursing intervention. The serum FSH level, serum estradiol concentration, serum LH level, endometrial thickness on the day of transplantation, pregnancy outcome, and psychological status in patients were observed. Results The serum LH level in the observation group was significantly higher than that in the control group (P<0.05). There was no significant difference in serum FSH level, serum estradiol concentration, endometrial thickness on the day of transplantation between the observation group and the control group (P>0.05). There was no significant difference in the clinical pregnancy rate and spontaneous abortion rate between the two groups (P>0.05). The SAS and SDS scores were lower in the observation group after the intervention, and the difference was significant from those of the control group (P<0.05). Conclusion Comprehensive nursing intervention had no significant effect on the pregnancy outcome of patients with artificial insemination by husband. The clinical pregnancy rate and spontaneous abortion rate are similar. But comprehensive nursing intervention can improve the serum LH level and improve the psychological status of patients.
الملخص
OBJECTIVE@#To construct short hairpin RNA interfering expression vector of TDRG1,and detect the specific interfering effect of TDRG1-shRNA expression vector on NTERA-2 cells.@*METHODS@#Oligos for short hairpin RNA targefing for TDRG1 were designed and connected to the expression vector pGPU6/GFP/Neo to construct the TDRG1 shRNA expression vector. The recombinant plasmid TDRG1-shRNA486, TDRG1-shRNA738, TDRG1-shRNA921 and lipofectamine ™2000 were used to generate and transfect shRNA into NTERA-2 cells. Expression of TDRG1 mRNA was assayed by RT-PCR.@*RESULTS@#TDRG1-shRNA expression vector was successfully constructed. TDRG1-shRNA486 was more effective in the suppression of TDRG1 with significant reduction of TDRG1 mRNA.@*CONCLUSION@#TDRG1-shRNA can interfere the expression of TDRG1 in NTERA-2 cells.