الملخص
0.05),but positively on PBD 7(r= 0.890,P
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Objective:To study the changes of energy expenditure regularities in Chinese burn adults.Methods:The resting energy expenditure(REE) was observed in 75 burn adults. Results: Burn metabolism can be divided into a slow-rise stage and a sharp-rise stage.The extent of respose and lasting time in hypermetabolism were proportional to the magnitude of total body surface area(TBSA).The peak level of REE may be 1.5-2.0 times as high as control.Even after the wound healing higher level of REE may lasts for a longer period,especially in severe burns.In case of sustaining lower REE and/or shart drop of REE are observed,the outcome is usually poor.After burning,the catabolism of protein is obviously increased,the utilization of glucose is decreasd,and the mobilization and utilization of lipid are markedly increased.Conclusion:The changes of energy expenditure regularities in Chinese burn adults are showed,the nutrition treatment should be actively given in severe burn patients.
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Objective: To study the relationship of resting energy expenditure(REE) with wound depth in burn patients and offer some guidance for the nutrition treatment of burn.Methods: Twenty-three burn patients with a similar total burn surface were randomly divided in accordance with the Ⅲ? wound area into Goups A,B,C and D,their total burn surface and Ⅲ?wound area were(66?18)% and(12?9)%,(59?18)% and(20?5)%,(62?6)% and(39?5)%,and(65?16)% and(54?4)%,respectively.Their REE was determined on PBD(postburn day) 1,2,3,7,14,21 and 28 with the indirect measuring method.The measured values of 13 normal adults were used for comparison.Results: The mean value of REE of each burn group was higher than the normal control.REE enhanced significantly with the increase of the Ⅲ? wound area.Conclusion: REE obviously increases in burn patients,and,with a similar total burn surface,the larger the Ⅲ? wound area,the higher REE.Therefore,increased calories should be furnished in the nutrition treatment of burn patients with a larger Ⅲ? wound area.
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Objective:Glutamine is the main oxidative fuel of the enterocyte which enters the enterocyte primarily via amino acid transporters.The aim of the test was to study the distributions and functions of glutamine transporters in IEC-6 cell line.Methods:The rat intestinal epithelial cell line(IEC-6) was incubated in vitro.The mRNA expression of different glutamine transporters,protein expression of system ASCT2,and the [3H]-L-glutamine uptake were measured.Results:The mRNA of system ASCT2,SN1,ATA1,LAT1,LAT2 was expressed and the protein expression of ASCT2 was also validated in IEC-6.In Na+-containing buffer,the velocity of Na+-dependent glutamine uptake was(164.07?37.94) fmol/(mg protein?10min).In Na+-free buffer,the velocity of glutamine uptake was(58.71?10.51)fmol/(mg protein?10min).With the saturate dosage of MeAIB,the velocity of glutamine uptake was(81.02 ?19.59) fmol/(mg protein?10min).Conclusion:There may be five kinds of glutamine transporters(ASCT2,SN1,ATA1,LAT1,and LAT2) in IEC-6 cell.The Na+-dependent transporter was the major contributor(64.22%) to glutamine total uptake in IEC-6.The contributions of system A and the remainder were 50.62% and 13.60%,respectively.The Na+-independent transporter was the lesser contributor(35.78%).
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<p><b>OBJECTIVE</b>To explore the effects of Tripterygium extract (LLZ) on postburn hypertrophic scar - derived fibroblasts, so as to provide us an experimental support for the treatment of postburn hypertrophic scar.</p><p><b>METHODS</b>Hypertrophic scar - derived fibroblasts were harvested and cultured in vitro. A serial concentrations of LLZ (5 x 10(-3), 5 x 10(-4), 5 x 10(-5), 5 x 10(-6) g/L) were added to the culture media. The cellular morphology, proliferating activity and the cytotoxicity of the drug were observed after 24 hours of culture.</p><p><b>RESULTS</b>The fibroblast morphology could be altered by any concentration of LLZ solution. Furthermore, the cell count and the cellular proliferating activity were all decreased obviously by the treatment of LLZ.</p><p><b>CONCLUSION</b>The cell morphology and proliferation activity of postburn scar-derived fibroblasts could all be inhibited by LLZ, and it was the result of its toxic effects.</p>
الموضوعات
Child , Child, Preschool , Female , Humans , Male , Burns , Drug Therapy , Pathology , Cell Division , Cell Size , Cells, Cultured , Cicatrix, Hypertrophic , Drug Therapy , Pathology , Dose-Response Relationship, Drug , Fibroblasts , Cell Biology , Phytotherapy , Plant Extracts , Pharmacology , Tripterygiumالملخص
Objective: This study was designed to investigate the variations of endotoxin,glutamine and TNF-? concentration in septic rats. Methods: A total of 90 adult male SD rats were divided as follows: control group(normal),experimental group(CLP).They were killed on hour 6,12,24,48,72 after surgery.Plasma,liver,small intestine and skeletal muscle were collected to measure the concentrations of glutamine in plasma and tissues,the levels of endotoxin and TNF-? in plasma. Results: In septic rats,plasma concentrations of endotoxin and TNF-? increased on hour 6,significantly increased on hour 12,attained the peak on hour 24,and decreased evidently on hour 48 and 72.Glutamine concentration in plasma elevated on hour 6 and 12,elevated significantly on hour 24 and 48,and decreased on hour 72.Glutamine concentration in liver increased on hour 6～12,increased significantly on hour 24,and decreased significantly on hour 48 and 72.Glutamine concentration in small intestine and skeletal muscle decreased on hour 6 and 12,and decreased significantly after 24 hour. Conclusion: During the early stage of sepsis,the plasma levels of endotoxin and TNF-? are increased significantly,the glutamine concentration in plasma and liver is also increased significantly,however,it was decreased evidently in skeletal muscle and small intestine.
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40% TBSA) were observed for 10 days after burn. The results showed that CRP, REE, UUN and UcA increased significantly postburn, CRP and REE had identical dynamic curves, and CRP had a direct bearing on REE, IL—1 might be an important medium of both. It is advisable to use CRP as a parameter to monitor burn hypermetabolism.
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The rate of resting energy expenditure (REE) was determined in 75 cases of burn patients with different total areas of burned wounds.Itwas found that.1.The rate of REE increased with the increase of the total area of burned wounds.2.When the total burned body surface area was equal,the rate of ERE increased more signficantly in the case with more extensive third degree burns.3.In the period immediately after the healing of the burned wounds,the rate of REE remained significantly higher than in the normal subjects.
الملخص
Thirty-two adult patients with burn wounds over 50% TBSA were randomized into the nutritioal and the control group.Energy supplement Ifor the patients of the nutritional group was given according to the burn nutrition formula of the Third Military Medical College as follows,Energy (kJ) /day=4184 (1000 cal)?body surface area (m2) +105 (25 cal)?burn surface area (%)glucose,fat:protein=55~60:20~30:15~20calorie (cal):nitrogen (g)=150~170:1The patients of the control were prescribed to eat the hospital diet freely.All the patients of the 2 groups were observed for 28 days postburn with their burn wounds similarly and properly treated.It was found that most of the nutritional indices observed were better in the nutritional group than in the control.
الملخص
Relative deficiency of serum insulin and insulin resistance of tissues are the main causes of postburn hypercatabolism.In order to evaluate the clinical effects of tolbutamide to improve postburn hypermetabolism,9 burn patients were given tolbutamide and observed for 10 days after the drug had gone through animal experiments.It was found that tolbutamide could improve postburn hyper-metabolism through following ways:1.To stimulate the secretion of insulin and enhance the effect of insulin.2.To reduce the insulin resistance of tissues and promote glucose utilization of skeletal muscles.3.To inhibit the secretion of glucagon.
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The resting energy expenditure (REE) and the relative ratio of carbohydrate,fat and protein were investigated on the 1st to the 28th dqy postburn in 75 adult burn victims with burn area ranged between 5~98% TBSA.It was found that there was high catabolism of fat and protein but relatively low consumption of carbohydrate after burns.The more severe the burn injury,the more marked the above changes.On the basis of the metabolic characteristics of burn patients and on the traditional dietary habits of Chinese,a reasonable relative ratio among carbohydrate,fat and protein,the 3 main nutritional elements,in the diet of burn patients was suggested,viz,carbohydrate:fat:protein=55~60:20~30:15~20.