Research progress in assisting in the diagnosis of early Alzheimer's disease through eye structure / 国际眼科杂志(Guoji Yanke Zazhi)

Alzheimer's disease(AD)is a common degenerative disease of the central nervous system in which neuropathological changes precede cognitive dysfunction and behavioral impairment. Currently, early diagnosis of AD is based on invasive and expensive testing techniques that are difficult to use widely in the clinical setting. Therefore, there is an urgent need for new markers to detect AD at an early stage. The eye, as an extension of the brain, has been found to show earlier onset of ocular pathologic changes in patients with AD compared to brain pathologic changes, such as retinal structural abnormalities, visual dysfunction, retinal abnormal protein accumulation, choroidal thickness changes, decreased corneal nerve fiber density, deposition of abnormal Aβ proteins in the lens, and pupillary light decreased sensitivity of response, etc. This article reviews the ocular pathologic changes in AD patients in recent years to provide new ideas for the early clinical diagnosis of AD.

Application of Rat Model of Neurotic Erectile Dysfunction in Experiment / 中国医学科学院学报

Erectile dysfunction (ED) refers to the persistent inability to achieve and/or maintain a sufficient erection of the penis to obtain a satisfactory sexual life,which affects the quality of life of the patients and their sexual partners.To decipher the pathophysiological mechanism of ED,researchers have established a variety of animal models and achieved a series of progress.The cavernous nerve (CN) of rodents,anatomically similar to that of humans,is cost-effective,thick,and easy to be identified,which has gradually become the mainstream of animal models.In this paper,we reviewed the modeling methods of the neurological ED caused by bilateral CN injury in rats in recent years,summarized the model evaluation indicators,and discussed the application and progress of ED models in basic experimental research.

Efficacy and safety of transumbilical single-incision laparoscopic appendectomy for acute complicated appendicitis:a single center,retrospective study / 临床外科杂志

Objective To investigate the efficacy and safety of transumbilical single-port laparoscopic appendicectomy in acute complicated appendicitis.Methods Retrospective analysis was conducted for the data of 1104 patients with complicated appendicitis who underwent emergency laparoscopic appendectomy at the Department of General Surgery of Aerospace Center Hospital from April 2014 to August 2022;among them,788 patients underwent transumbilical single-port laparoscopic appendectomy(SILA)and 316 cases underwent traditional three-port laparoscopic appendectomy(LA);the operation time,intraoperative blood loss,leukocyte value on the first day after surgery,postoperative exhaust time,hospital stay,postoperative pathology and postoperative complications were statistically analyzed.Results The surgical duration of the single hole laparoscopic appendectomy(SILA)group was(68.26±22.29)minutes,intraoperative blood loss was(15.93±13.10)ml,postoperative exhaust time was(2.29±0.52)days,and white blood cells were(11.12±1.67)× 109/L on the first day after surgery,and the surgical duration of the hree hole laparoscopic appendectomy(LA)groupwas(66.47± 20.40)minutes,intraoperative blood loss was(16.65±12.98)ml,postoperative exhaust time was(2.23±0.58)days,and white blood cells were(11.35±1.54)× 109/L on the first day after surgery,there was no statistically significant difference in the data between each group(P＞0.05).After 1 month of follow-up,no incisional hernia and other complications occurred in the two groups,the cosmetic effect of abdominal incision in SILA group was satisfactory,the hospitalization time of SILA group was(4.60± 1.18)days,which was shorter than that in the traditional LA group(4.93±1.71)days,and the difference was statistically significant(P＜0.05).Conclusion Based on proficiency in traditional LA operations,SILA is safe and viable;in addition to the hidden aesthetic function of scars,it does not prolong the operation time and increase the risk of postoperative complications.

Effect of pulsed electric field on reactive oxygen species generation in pancreatic cancer cells / 中华肝胆外科杂志

Objective:To investigate the changes of reactive oxygen species (ROS) in pancreatic cancer cells under the effect of pulsed electric fields (PEF).Methods:Murine-derived pancreatic cancer cells Panc02 were treated with PEF at electric field strengths of 0, 250, 500, 750, and 1 000 V/cm, respectively. The intracellular ROS generation patterns under the different field strengths and at different times after the PEF were investigated in vitro by flow cytometry and immunofluorescence, meanwhile exploring the apoptosis of murine and human pancreatic cancer cells under different field strengths. Twenty 6- to 8-week-old male C57BL/6 SPF mice were prepared as orthotopic pancreatic cancer models and divided into five groups of four mice each: 250 V/cm PEF group, 500 V/cm PEF group, 750 V/cm PEF group, 1 000 V/cm PEF group, and sham operation group. ROS expression in the residual tumor tissues of mice in each group was detected by immunofluorescence.Results:Under the 500 V/cm, 750 V/cm and 1 000 V/cm electric field strength, the proportion of cells with intracellular ROS expression was decreased after 6 h, 12 h, 24 h and 48 h of the PEF compared with 2 h after the PEF, and the differences were statistically significant (all P<0.05). Compared with 0 V/cm PEF group, ROS expression increased in Panc02 cells treated with 500 V/cm and 750 V/cm PEF groups, and the differences were statistically significant (all P<0.05). Compared with 250 V/cm PEF group under the same time, ROS in Panc02 cells treated with 500 V/cm and 750 V/cm electric field strengths increased, and the differences were statistically significant (all P<0.05). The proportions of apoptosis of both Panc02 cells and MIA-PaCa-2 cells increased with rising field strength and peaked at the field strength of 750 V/cm. Compared with the sham-operated group, the expression of ROS was increased in pancreatic cancer tissues of mice in the 500 V/cm PEF-treated group (16.65±6.01 vs. 2.38±1.21, t=-6.53) and 750 V/cm PEF-treated group (16.54±4.41 vs. 2.38±1.21, t=-6.48), and the differences were statistically significant in both cases (both P<0.001). Conclusion:PEF treatment was able to increase the level of ROS in both pancreatic cancer cells and tissues, and more ROS were produced when the electric field strength was 500 and 750 V/cm.

Microsurgical thinned anterolateral thigh perforator flap to repair soft tissue defects of foot and ankle / 中国骨伤

OBJECTIVE@#To explore method and clinical effect of microsurgical thinned anterolateral thigh perforator flap to repair soft tissue defects of foot and ankle.@*METHODS@#From March 2017 to January 2022, totally 20 patients with soft tissue defects of ankle joint were treated with micro-thinning anterolateral perforator flap for free transplantation, included 13 males and 7 females, aged from 22 to 58 years old with an average of (36.45±12.36) years old. The size of flap ranged from 8.0 cm×5.0 cm to 20.0 cm×12.0 cm. Before operation, perforating vessels on the anterolateral thigh region were detected and marked with a portable Doppler detector. For the defect width less than 8 cm, 11 patients were repaired with a single flap. For the defect width more than 8 cm, the wound could not be sutured directly, and the lobulated flap technique was used in 9 patients, the width was converted to length, and the donor site was closed directly. Under the microscope, all flaps were thinened in a stepwise manner from the center of the pedicle to the periphery. After operation, survival of the flap, the shape, texture, sensory function recovery were observes, and recovery of foot function was evaluated by Maryland foot function evaluation standard.@*RESULTS@#All 20 patients with microsurgical thinned anterolateral thigh perforator flaps were survived. Venous crisis occurred in 1 patient due to subcutaneous hematoma, after removal of the hematoma, the crisis was relieved and the flap survived successfully. The wounds in the donor and recipient sites healed well, and only linear scars left in the donor sites. Twenty patients were followed up for 3 to 26 months after operation, good shape of flaps without bloated, and good texture. The two-point discrimination of free flaps ranged from 9.0 to 16.0 mm, and the protective sensation was restored. The ankle flexion and extension function recovered well and patients could walk normally. According to Maryland foot function evaluation standard, 8 patients got excellent result, 10 patients good and 2 middle.@*CONCLUSION@#Microsurgical thinned anterolateral thigh perforator flap is an ideal method to repair soft tissue defects in functional area of foot and ankle, with good appearance and texture of the flap, no need for re-plastic surgery, reduced hospitalization costs, and less donor site damage.

Evaluation of myocardial perfusion and systolic function in patients with coronary heart disease by myocardial contrast echocardiography and 2-dimensional speckle tracking imaging / 中南大学学报(医学版)

OBJECTIVES@#The high prevalence and high fatality rate of coronary heart disease seriously endanger the safety of human life. The key to its treatment is to restore the perfusion of the narrowed coronary arteries as soon as possible. Two-dimensional echocardiography is limited for assessment of postoperative myocardial function. Myocardial contrast echocardiography (MCE) and 2-dimensional speckle tracking imaging (2D-STI) possess good value in assessing myocardial perfusion and systolic function. We used MCE and 2D-STI to explore the changes of myocardial perfusion and systolic function after percutaneous coronary intervention (PCI) in patients with coronary heart disease, and to evaluate the clinical value of MCE and 2D-STI.@*METHODS@#Twenty patients with coronary heart disease undergoing PCI were selected. MCE was performed before and one day after PCI, and 2D-STI and conventional ultrasound were performed before and a month after PCI. The recovery of left ventricular wall motion was used as a standard to evaluate the ability of MCE semi-quantitative analysis and to predict the recovery of myocardial segment motion.@*RESULTS@#The quantitative value of MCE (signal intensity of contrast medium in plateau phase, slope of curve, and their product) one day after PCI and the contractile function of one month after PCI were significantly improved (all @*CONCLUSIONS@#MCE and 2D-STI can evaluate the improvement of myocardial perfusion and systolic function in patients with coronary heart disease after PCI, and MCE semi-quantitative analysis can effectively predict the ability of ventricular wall recovery.

Current Situation of Clinical Feature and Gene Phenotype of Young Adult Lung Cancer / 中国肺癌杂志

Young adult lung cancer is defined as a group of patients refers to whose onset age is less than 40 years old and ≥18 years old. Compare with elder lung cancer, the clinical symptoms of them are not typical, the stage is usually late at the time of discovery, and most of them have regional lymphatic metastasis or distant metastasis. Current study found that young adult lung cancer has a relatively unique genetic background, the abundance of tumor-driving genes is high, and it is closely related to its clinical manifestation and prognosis. Young adult lung cancer is the focus of attention in the field of cancer in recent years. This article reviewed the literature on the clinical features, gene phenotypic characteristics and prognosis of young adult lung cancer in order to provide provide some references and clues for the study on young adult lung cancer.

Research Progress of Catheter-based Irreversible Electroporation for Tissue Ablation / 中国医疗器械杂志

Irreversible electroporation (IRE) is an emerging tissue ablation technique. Compared with thermal ablation technique such as radiofrequency, IRE can achieve focal ablation in a shorter time without heat sink effect while sparing the tissue scaffold. IRE has been demonstrated to be a feasible therapeutic modality for the liver, pancreatic, and prostatic cancer. In recent years, several studies regarding of catheter-directed IRE for digestive tract, bronchus, urinary tract, and myocardium have been performed, which preliminarily demonstrated the safety and efficacy of IRE for tissue ablation under endoscopic or interventional technique. This study summarized the research progress of catheter-directed IRE for tissue ablation. The critical technique and future direction of catheter-based IRE are prosp.

Medical Philosophy in Ancient Greece and Its Enlightenment / 中国医学伦理学

Medical philosophy has important guiding significance for the development of medicine. Ancient Greek medical philosophy has rich connotation and strong vitality. In the study of ancient Greek medical philosophy and combined with"the positioning of basic medicine"and"doctor-patient relationship"and other outstanding is-sues in the current development of medicine, we found that the rational examination of medical theory and medical treatment in ancient Greek medical philosophy, ways of thinking and analytical methods of medical philosophy and the character that physician should have had an important value in the development of modern medicine. We should pay attention to basic medical research,pursue innovative thinking, be good at using critical thinking and pay atten-tion to the training of humanistic spirit among medical workers.

The prospects of ferroptosis in the pathogenesis of liver disease / 药学学报

Ferroptosis is a novel type of cell death which induced by iron-dependent lipid peroxidation accumulation. This type of cell death is significantly different from other cell death in terms of morphology, genetics and biochemistry. It has been reported that ferroptosis is involved in a variety of human diseases, particularly in liver diseases. Therefore, screening and studying of inhibitors or activators of ferroptosis may provide novel strategies for prevention and treatment of liver diseases. This review provides the biological characteristics and regulatory signaling pathways of ferroptosis, as well as the relationship between ferroptosis and liver diseases, which will contribute to new insight into the pathogenesis of liver diseases.

Study of vascular endothelial growth factor and transforming growth factor beta1 genes regulate the mineral-related genes in human cells from apical papilla / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To clone the VEGF165 gene and to construct eucaryotic expression vector, investigate the effect of overexpressed VEGF165 and transforming growth factor beta1 (TGFbeta1) on the mineral-related genes in human cells from apical papilla.</p><p><b>METHODS</b>Total RNA of ECV304 cell was extracted. The VEGF165 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and then was subcloned into eucaryotic expression vector pcDNA3.1hisA to construct the recombinant vector pcDNA3.1hisA-VEGF165. After being identified by digestion and DNA sequencing, pcDNA3.1hisA-VEGF165, and pcDNA3.1hisA-TGFbeta1 were transfected into human cells from apical papilla Then the efficiency of gene transfection and the expression of bone sialoprotein (BSP), dentin sialophosphoprotein (DSPP), osteocalcin (OCN), dentin matrix protein 1 (DMP1) were detected by Real-Time polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Cloned VEGF165 gene sequences and inserted into expression vector of the VEGF165 sequences showed 100% homology related to the sequence in GenBank database. VEGF165 and TGFbeta1 mRNA were upregulated after transfection. The expression of DSPP mRNA were significantly increased in each experiment group (P < 0.05). The expression of OCN mRNA were increased significantly in the group transfected with pcDNA3.1hisA-TGFbeta1 and transfected with two plasmids (P < 0.05). The expression of BSP mRNA were not varying (P > 0.05), while no expression of DMP1 mRNA in each experiment group.</p><p><b>CONCLUSION</b>The recombinant eucaryotic expression vector of pcDNA3.1hisA-VEGF165 was constructed successfully. VEGF165 and TGFbeta1 can induce the expression of most mineral-related genes and they may play a key role during the differentiation of human cells from apical papilla.</p>

Evaluation of the efficiency of different instrumentation for root canal retreatment using micro-computed tomography / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To establish the three-dimensional model of human permanent premolars based on Micro-computed tomography (Micro-CT) data, and evaluate the efficiency quantitatively of two different instrumentations for root canal retreatment.</p><p><b>METHODS</b>Forty extracted permanent premolars for the reason of orthodontic treatment were collected, prepared by using ProTaper Ni-Ti files and filled by cold gutta pertscha lateral condensation technique. The subjects were scanned by Micro-CT. Forty teeth were randomly divided into two groups and retreated by K-Flexo files and ProTaper Universal retreatment system respectively. Then all subjects were scanned again, and the mean percentage of remaining filling materials and the scores of remaining filling materials presented in upper, middle and apical 1/3 of the canal were calculated.</p><p><b>RESULTS</b>Mean percentage of the remaining filling materials by K-Flexo files was lower than that by ProTaper Universal retreatment system (P=0.005). The scores demonstrated that K-Flexo files had greater efficiency than ProTaper Universal retreatment system when retreating the apical 1/3 of canal (P<0.05).</p><p><b>CONCLUSION</b>This study shows that both techniques could not remove filling materials completely.</p>

Incidence of C-shaped root canal systems in mandibular second molars in the native Chinese population by analysis of clinical methods / 国际口腔科学杂志·英文版

The aims of the study were to investigate the incidence of C-shaped root canal systems in mandibular second molars in a native Chinese population using radiography and clinical examination under microscope and to compare the relative efficacies of these methods. For the recognition of C-shaped root canal system, 1 146 mandibular second molars were selected and examined. Teeth with C-shaped canal systems were categorized by using the radiographic classification criteria and the modified Melton's method. C-shaped canals were identified in 397 (34.64%) mandibular second molars by radiography (type I, 31.23%; type II, 38.29%; type III, 30.48%). Clinical examination showed that 449 (39.18%) cases exhibited C-shaped canal systems (C1, 22.94%; C2, 48.11%; C3a, 15.59%; C3b, 13.36%). As for the result of the radiographic and clinical combined examination, C-shaped root canals were found in 473 (41.27%) mandibular second molars (C1, 21.78%; C2, 45.67%; C3a, 16.70%; C3b, 15.86%). The incidence of C-shaped root canal diagnosed by radiographic method was statistically different from that by clinical examination and the combined examination (P<0.05). The study indicated a high incidence of C-shaped canal system in a Chinese population. The combination of microscopic and radiographic examination is an effective method in identifying the C-shaped root canal system.

MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line / 南方医科大学学报

<p><b>OBJECTIVE</b>To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).</p><p><b>METHODS</b>The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.</p><p><b>RESULTS</b>5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.</p><p><b>CONCLUSIONS</b>MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.</p>

A new bibenzyl derivative from Bletilla striata / 药学学报

<p><b>AIM</b>To study the chemical constituents of Bletilla striata.</p><p><b>METHODS</b>Various column chromatographies with silica gel and Sephadex LH-20 were employed for the isolation and purification. The structures of the compounds were elucidated on the basis of spectral analyses and chemical methods.</p><p><b>RESULTS</b>Three compounds were isolated from the roots of Bletilla striata (Thunb.) Reichb. f. and identified as 5-hydroxy-4-(p-hydroxybenzyl)-3'-3-dimethoxybibenzyl (I), schizandrin (II), 4,4'-dimethoxy-(1,1'-biphenanthrene)-2,2',7,7'-tetrol (III).</p><p><b>CONCLUSION</b>Compound I is a new bibenzyl derivative and II was isolated from this plant for the first time.</p>