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Objective To analyze the differences among electrical damage, burns and abrasions in pig skin using Fourier transform infrared microspectroscopy (FTIR-MSP) combined with machine learning algorithm, to construct three kinds of skin injury determination models and select characteristic markers of electric injuries, in order to provide a new method for skin electric mark identification. Methods Models of electrical damage, burns and abrasions in pig skin were established. Morphological changes of different injuries were examined using traditional HE staining. The FTIR-MSP was used to detect the epidermal cell spectrum. Principal component method and partial least squares method were used to analyze the injury classification. Linear discriminant and support vector machine were used to construct the classification model, and factor loading was used to select the characteristic markers. Results Compared with the control group, the epidermal cells of the electrical damage group, burn group and abrasion group showed polarization, which was more obvious in the electrical damage group and burn group. Different types of damage was distinguished by principal component and partial least squares method. Linear discriminant and support vector machine models could effectively diagnose different damages. The absorption peaks at 2 923 cm-1, 2 854 cm-1, 1 623 cm-1, and 1 535 cm-1 showed significant differences in different injury groups. The peak intensity of electrical injury's 2 923 cm-1 absorption peak was the highest. Conclusion FTIR-MSP combined with machine learning algorithm provides a new technique to diagnose skin electrical damage and identification electrocution.
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Animals , Algorithms , Fourier Analysis , Least-Squares Analysis , Machine Learning , Swineالملخص
Endometriosis(EMs), whose pathogenesis is complicatedand is not fully understood, is a common gynecological disease. The association between gene polymorphism and EMs is a hot spot of research for its pathogenesis and pathogenic mechanism, which provides a research basis for detection of susceptible disease loci inhigh-risk groups and the identification and genetic analysis ofdiseases and related genes, and offers more help for EMs patients in clarifying diagnosis at source and improving therapy outcome. This paper reviews the research status of EMs gene polymorphism.
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Objective To analyse the relationship between Fourier transform infrared (FTIR) spectrum ofrat's spleen tissue and postmortem interval (PMI) for PMI estimation using FTIR spectroscopy combinedwith data mining method. Methods Rats were sacrificed by cervical dislocation, and the cadavers were placed at 20 ℃. The FTIR spectrum data of rats' spleen tissues were taken and measured at different time points. After pretreatment, the data was analysed by data mining method. Results The absorption peak intensity of rat's spleen tissue spectrum changed with the PMI, while the absorption peak position was unchanged. The results of principal component analysis (PCA) showed that the cumulative contribution rate of the first three principal components was 96%. There was an obvious clustering tendency for the spectrum sample at each time point. The methods of partial least squares discriminant analysis (PLS- DA) and support vector machine classification (SVMC) effectively divided the spectrum samples with different PMI into four categories (0-24 h, 48-72 h, 96-120 h and 144-168 h). The determination coefficient (R2) of the PMI estimation model established by PLS regression analysis was 0.96, and the root mean square error of calibration (RMSEC) and root mean square error of cross validation (RMSECV) were 9.90 h and 11.39 h respectively. In prediction set, the R2 was 0.97, and the root mean square error of prediction (RMSEP) was 10.49 h. Conclusion The FTIR spectrum of the rat's spleen tissue can be effectively analyzed qualitatively and quantitatively by the combination of FTIR spectroscopy and data mining method, and the classification and PLS regression models can be established for PMI estimation.
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OBJECTIVES@#To analyse the Fourier transform infrared (FTIR) spectral data of renal tissue at different temperatures in rats after death, and to explore the effects of temperature on the FTIR spectral characteristics of renal tissue.@*METHODS@#The rats were sacrificed by cervical dislocation and placed at 4 ℃, 20 ℃ and 30 ℃. The FTIR spectral data of renal tissue were collected at different time points and analysed by data mining method.@*RESULTS@#The principal component analysis (PCA) results showed that there were significant trends of clustering in the samples of partial time point at 4 ℃, 20 ℃ and 30 ℃. Partial least square (PLS) regression models were established with the spectral data at three temperature groups. The performance of PLS regression models in 20 ℃ and 30 ℃ groups were more superior than that in 4 ℃ group, and the stability of the model in 20 ℃ group was better than that in 30 ℃ group.@*CONCLUSIONS@#There are differences in the FTIR spectral characteristics of renal tissue of rats after death at different temperatures. Temperature has a major impact on the performance of FTIR spectral PLS regression model. Therefore, in order to improve the accuracy of postmortem interval estimation, the effects of temperature on the model should be considered in the related study by spectral method.
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Animals , Rats , Autopsy , Death , Postmortem Changes , Spectroscopy, Fourier Transform Infrared/methods , Temperatureالملخص
Objective To assessment the effect of low dose ionizing radiation on liver function .Methods According to predefined inclusion , exclusion criteria , the relevant literatures were reviewed and qualified literatures were evalu -ated.Based on total number of liver function examination , number of abnormal liver function in radiation group and control group , the OR as the effect size , then choose an appropriate model by the heterogeneity test for the analysis . Results The combined OR is 1.59, 95%CI 1.25-2.02.Conclusions Long term exposure to low dose-ionizing radi-ation has significant outcome shown by higher rate of liver function abnormality .
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OBJECTIVES@#To explore the correlation between early postmortem interval (PMI) and eight RNA markers of rat's brain at different temperatures.@*METHODS@#Total 222 SD rats were randomly divided into control group (PMI=0 h) and four experimental groups. And the rats in the experimental groups were sacrificed by cervical dislocation and respectively kept at 5 ℃, 15 ℃, 25 ℃ and 35 ℃ in a controlled environment chamber. The RNA was extracted from brain tissues, which was taken at 9 time points from 1 h to 24 h postmortem. The expression levels of eight markers, β-actin, GAPDH, RPS29, 18S rRNA, 5S rRNA, U6 snRNA, miRNA-9 and miRNA-125b, were detected using real-time fluorescent quantitative PCR, respectively. Proper internal reference was selected by geNorm software. Regression analysis of normalized RNA markers was performed by SPSS software. Mathematical model for PMI estimation was established using R software. Another 6 SD rats with known PMI were used to verify the mathematical model.@*RESULTS@#5S rRNA, miR-9 and miR-125b were suitable as internal reference markers for their stable expression. Both β-actin and GAPDH had well time-dependent degradation patterns and degraded continually with prolongation of PMI in 24 h postmortem. The mathematical model of the variation of ΔCt values with PMI and temperature was set up by R software and the model could be used for PMI estimation. The average error rates of model validation using β-actin and GAPDH were 14.1% and 22.2%, respectively.@*CONCLUSIONS@#The expression levels of β-actin and GAPDH are well correlated with PMI and environmental temperature. The mathematical model established in present study can provide references for estimating early PMI under various temperature conditions.
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Animals , Rats , Actins/metabolism , Autopsy , Brain/pathology , Genetic Markers , MicroRNAs , Models, Theoretical , Postmortem Changes , RNA Stability , RNA, Small Nuclear , Rats, Sprague-Dawley , Real-Time Polymerase Chain Reaction , Regression Analysis , Software , Temperature , Time Factorsالملخص
Objective:To investigate the status of blood mineral content of children aged 3 to 12 years in Chinese cities and countryside and explore the possible related influencing factors .Methods: The multistage stratified cluster random sampling was used to select one kindergarten and one primary school in seven cities ( Beijing and Guangzhou and so on ) and two towns randomly .Firstly, we selected one bottom class , middle class , top class in one kindergarten and one second grade and fifth grade in one pri-mary school randomly .Then all of the healthful students of these classes were investigated and the blood mineral content of calcium , magnesium, lead, iron, copper, and zinc were detected.Results:In the re-search, 1 842 students were investigated .The means of calcium, magnesium, lead, iron, copper, and zinc were in the standard range .The blood lead content of the boys was higher than that of the girls .The blood mineral content of different ages had statistical significance .The blood calcium and blood copper contents of the preschool children were higher than those of the school children .However , the school children had significantly higher blood lead , iron, and zinc contents in comparison with those of the pre-school children .The incidences of iron deficiency and zinc deficiency were 35.5%and 39.6%, respec-tively .The incidence of iron deficiency and zinc deficiency of different ages had statistical significance , and with the age increasing , the incidence showed a decreasing trend .The incidences of iron deficiency and zinc deficiency of children whose age was more than or equal to 3 years and less than 4 years were up to 47.1%and 64.6%, respectively.The incidence of iron deficiency of the children in the countryside area was significantly higher than those in the first-tier cities.However, the incidence of zinc deficiency of the children in the countryside area was significantly lower than those in the first-tier cities and second-tier cities.Conclusion:The status of iron deficiency and zinc deficiency of children aged 3 to 12 years in Chinese cities and countryside were still serious .We should pay more attention to the nutrition interven-tional research on iron and zinc .
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Objective To analyze the electromyogram (EMG) changes of patients with acute flaccid paralysis (AFP) in Hetian Xinjiang.Methods Twenty-six patients with acute poliogmyelitis,admitted to our hospital from July 2011 to September 2012,were chosen in our study; their clinical data were analyzed,and they all underwent EMG examination.Results Most patients had contact with respiratory virus or digestive virus prior to illness onset.Most of patients had flaccid paralysis,muscle strength between 0 and 2 grades,tendon reflex being abated and no sensory disturbance.Fourteen patients had positive blood stool.Motor nerve conduction velocity (MCV) was abnormal,H-reflex was decreased or not educed and sensory nerve conduction velocity was normal in lower limbs of the 26 patients; EMG showed myogenic abnormality.Nerve conduction velocity was normal or low and Fwave was normal or decreased in upper limbs; EMG showed neurogenic abnormality in five of the 26 patients.Poor prognosis was concluded in all the patients.Conclusion EMG has important diagnosis value in AFP induced by polio wild-type I.
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Objective To identify and analyze the species of vaginal lactobacilli between patients with bacterial vaginosis (BV) and healthy women at childbearing age in Inner Mongolia. Methods From Jun. 2008 to Dec. 2008, 203 Mongolian healthy women, 74 Han healthy women and 102 Mongolian patients with BV from 3 pastoral areas were enrolled in this study. Isolation and culture of lactobacilli from vaginal wall were performed by modified culture medium. DNA of lactobacilli were extracted and sequenced. H2O2 were detected by TMB-HRP-MRS. Results(1)The rate of lactobacilli identification were 76.8%(156/203) in Mongolian healthy women and 21.6% (22/102) in Mongolian patients with BV, which reached statistical difference(P<0.01).Lactobacilli identification in Han healthy women [82.4%(61/74)] did not show significant difference with that of Mongolian healthy women (P>0.05). (2) The total of 193 strains and 11 species of Lactobacillus were detected in 203 Mongolian healthy women. Meanwhile,22 strains and 4 species of Lactobacillus were found in 102 Mongolian BV cases.(3)The rate of H2O2 generating Lactobacilli was 27.3% (6/22) in Mongolian BV patients and 75.7% (56/74)in Mongolian healthy women, which showed statistical difference(P<0.05). Conclusions The rate of Lactobacillus was not related with the race of women in pastoral area in Inner Mongolian. The amount of lactobacilli and H2O2 generating Lactobacilli in the vagina of BV patients was remarkably lower than those of healthy women at childbearing age.
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OBJECTIVE@#To explore the features of genetic differentiation and gene flow of ten minorities in Yunnan province according to nine CODIS short tandem repeat(STR) loci (CSF1PO, FGA, THO1, TPOX, v WA, D3S1358, D5S818, D7S820 and D13S317).@*METHODS@#Heterozygosity and parameters of population differentiation such as F, theta, f and Gst at each locus were calculated. DA genetic distance and fixation index Fst were calculated by Phylip 3.6 and Arlequin 3.0 software, respectively. Phylogenetic trees were constructed by Mega 3.0, and the patterns of gene flow were analyzed by R-matrix model.@*RESULTS@#It showed that average genetic heterogeneity in ten minorities was above 0.7. Significant difference was found for most of the loci in genetic differentiation. Phylogenetic tree analysis showed that the ten minorities were divided into two clusters. The results of the R-matrix analysis showed that the gene flow of Yi and Dai minorities were higher than that of other minorities, while the pattern of gene flow of Dulong minority demonstrated some of the isolation.@*CONCLUSION@#Nine STR loci commonly used in forensic identification show a high polymorphism. Heterozygosity can be used for investigating genetic differentiation and gene flow of minority. The ten minorities in Yunnan are independent populations, while the level of differentiation is not high. The relationship in evolution is not far from each other and shows a widely gene flow among the minorities.
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Humans , Alleles , China/ethnology , Cluster Analysis , Ethnicity/genetics , Gene Flow , Gene Frequency , Genetic Structures , Genetics, Population , Genotype , Microsatellite Repeats/genetics , Models, Genetic , Pedigree , Phylogeny , Polymorphism, Genetic , Regression Analysisالملخص
OBJECTIVE@#FTIR (Fourier transform infrared) spectroscopy was applied to observe the process of postmortem degradation in rats' cardiac muscle and provided a new method for the estimation of post-mortem interval (PMI).@*METHODS@#The rats were sacrificed by cervical dislocation and the bodies were kept in a controlled environmental chamber set at (20 +/- 2) degrees C. The FTIR spectra was applied to measure the changes of different chemical group from rats' left ventricle muscle at the different time point postmortem.@*RESULTS@#There were not obvious changes for the main FTIR absorbance peaks. But the different FTIR absorbance at the wave-number (cm(-1)) indicated the three types: increase, decrease, stable. The various absorbance ratios also demonstrated the similar changes.@*CONCLUSION@#FTIR spectroscopy may be potentially used as an effective method for estimating PMI in forensic practice using cardiac muscle tissue.
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Animals , Male , Rats , Autopsy , Fatty Acids/metabolism , Forensic Pathology , Models, Animal , Myocardium/metabolism , Nucleic Acids/metabolism , Postmortem Changes , Rats, Sprague-Dawley , Spectroscopy, Fourier Transform Infrared/methods , Time Factorsالملخص
Estimation of the postmortem interval (PMI) is a practical task in daily forensic casework. Researches on PMI is an important practical project in forensic field. Estimation of the time since death is influenced by internal and external, antemortem and postmortem factors, thus the old methods have limitations. Fourier transform infrared (FTIR) spectroscopy has been applied to study the pure protein, nucleic acid and carbohydrate and to detect the changes in complex cells and tissues. At present because the powerful software has could be used to achieve the spectrum transformation, smoothing, baseline correction and normalization, it is possible to analyze the samples quantitatively with the FTIR which has been applied in the biology and clinical medicine. This paper has reviewed the mechanism of FTIR and its application in biomedicine. The postmortem FTIR spectral changes were also discussed, which showed its potential for estimating PMI.
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Animals , Humans , Rats , Forensic Pathology/methods , Glycogen/biosynthesis , Kidney Cortex/metabolism , Lung/metabolism , Muscle, Skeletal/metabolism , Myocardium/metabolism , Nucleic Acids/metabolism , Postmortem Changes , Spectroscopy, Fourier Transform Infrared/methods , Spleen/metabolism , Temperature , Time Factorsالملخص
Objective To investigate the dynamics of the level of S100 in cerebrum, brainstem, and serum following the diffuse brain injury in rats and provide the experimental evidences for estimating injury time. Methods ELISA was used to determine whether S100 protein is changed after diffuse brain injury in rats. Forty rats were sacrificed at 0.5 hour, 2 hours, 4 hours, 12 hours, 24 hours, 3 d and 7 d after diffuse brain injury and normal rats as control. Results The level of S100 in cerebrum, brainstem, and serum increased, followed by a decrease, and then further increased. The level of S100 could be detected to increase at 30 minutes and reached the peak at 4 hours after DBI. The level decreased gradually to the normal at 1d and till 3 d formed the second peak. The level returned to the normal at 7d following injury again. In the postmortem injury groups, there were no significant changes compared to the control group. Conclusion The present study showed that the time-dependent expression of S100 is obvious following diffuse brain injury in rats and suggested that S100 will be a suitable marker for diffuse brain injury age determination.
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OBJECTIVE@#To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science.@*METHODS@#The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference.@*RESULTS@#The length of PCR products were 807 bp and 962 bp respectively. Seven genotypes in each locus were detected. AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype, while AAAACCCCCCCCCCCCC was firstly reported in Han population. CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype. 15 haplotypes were detected among 114 samples. The GD value was 0.6309. The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%.@*CONCLUSION@#The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.
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Humans , Alleles , China , DNA Primers , DNA, Mitochondrial/genetics , Gene Frequency , Genetics, Population , Genotype , Haplotypes/genetics , Polymerase Chain Reaction , Polymorphism, Genetic , Sequence Analysis, DNAالملخص
OBJECTIVE@#To validate the genetic characteristics and distribution of DXS7424-DXS101 on X chromosome in Han population.@*METHODS@#DXS7424 and DXS101 loci were genotyped by PCR, PAGE and silvers stain methods. Their genetic parameters were analyzed by Arlequin software.@*RESULTS@#There were 37 haplotypes detected in 151 Han unrelated males. The frequencies ranged from 0.0066 to 0.1391, with a GD value of 0.9453 and a DP value of 0.9389. Haplotypes 16-23 were the most common haplotypes in Han population.@*CONCLUSION@#Analysis of combined DXS7424 and DXS101 haplotypes appears to be a powerful means in population genetics and forensic practice for determination of identity and paternity.
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Humans , Male , Alleles , China/ethnology , Chromosomes, Human, X/genetics , Gene Frequency , Genetic Linkage/genetics , Genetics, Population , Haplotypes , Polymerase Chain Reaction , Polymorphism, Genetic , Tandem Repeat Sequencesالملخص
Objective To study the antiangiogenesis effect of active components T3 and M2 from single Chinese herbal medicine No.6 and No.10 with invigorating the circulation of blood in Chinese medicine theory. Methods human vascular endothelial cell (EC) was cultivated in vitro, the MTT assay and CAM experiment were carried out. Results T3 and M2 showed very significant inhibitory effect on EC (P
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<p><b>OBJECTIVE</b>To investigate the alleles and genotypes frequency of 10 short tandem repeat (STR) loci (DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378) on X chromosome of Chinese Hui nationality population.</p><p><b>METHODS</b>The study of 10 STR loci was performed by using the techniques of PCR, polyacylamide gel electrophoresis and silver staining.</p><p><b>RESULTS</b>Among unrelated Hui individuals, the allele numbers of 10 STR loci DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378 were 9, 8, 4, 6, 6, 6, 4, 4, 5 or 5 respectively; the numbers of genotypes were 17, 22, 7, 14, 14, 15, 6, 7, 12 or 8 respectively. The distribution of genotypes from these 10 STR systems fitted the Hardy-Weinberg equilibrium (P> 0.05). Polymorphisms information content of 10 STR loci, except for DXS7133 (0.48) and DXS7423 (0.48), ranged from 0.54 (DXS6799) to 0.80 (DXS6789); the power of discrimination were from 0.89 (DXS7133, DXS7423) to 0.99 (DXS6789, DXS7132, DXS101).</p><p><b>CONCLUSION</b>The loci of 10 STR on chromosome X are appropriate for individual identification, paternity testing involving a female child and for study on related disease.</p>
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Child , Female , Humans , Male , Alleles , China , Chromosomes, Human, X , Genetics , Electrophoresis, Polyacrylamide Gel , Gene Frequency , Genotype , Linkage Disequilibrium , Microsatellite Repeats , Genetics , Polymerase Chain Reaction , Polymorphism, Genetic , Geneticsالملخص
OBJECTIVE@#To investigate the dynamics of the induction of S100beta in different parts of rat brain following the diffuse brain injury.@*METHODS@#Immunohistochemistry and auto-image analysis were to determine the expression of astroglial S100beta after diffuse brain injury in rats. Forty rats were distributed into groups according to injury time of 30min, and2,4,12,24h, and 3,6 d after diffuse brain injury, and normal rats as control.@*RESULTS@#The number of S100beta positive cells in the four areas increased significantly followed by a decrease, and then a further increase. The expression of S100beta could be detected increasing in 2h, and increased significantly in 4h, and it reached apex 12h after DBI, and decreased gradually to the level less than normal 3d, and returned to normal 7d following injury. In the postmortem injury groups, there were no significant changes in anti-S100beta immunoreactivities in four areas of brain compared to the control group.@*CONCLUSION@#The present study showed the time-dependent expression of S100beta is obvious following diffuse brain injury, and suggested S100beta be suitable as a marker for brain injury age determination.
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Animals , Female , Male , Rats , Brain/pathology , Brain Edema/pathology , Brain Injuries/pathology , Immunohistochemistry , Nerve Growth Factors/analysis , Neuroglia/metabolism , Random Allocation , Rats, Sprague-Dawley , S100 Calcium Binding Protein beta Subunit , S100 Proteins/analysis , Staining and Labeling , Time Factorsالملخص
S100beta is one kind of the calcium binding proteins. As growth factor of neuraxon, it is excreted by neuroglial cell, and distributing in nerve tissue extensively. Although S100beta has very important values neurophysiological, it also has neurotoxicity with excreting overmuch. Concentration of S100beta changes regularity in serum after the brain injury. In addition, it has a close relations with the degree of brain damage, which can be regarded as the neural new marker of biochemistry after brain damage. The advances of S100beta protein, in the research on neurophysiological values and its application for nerve tissue injury, disease were reviewed.
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Humans , Alzheimer Disease/pathology , Biomarkers/blood , Brain Injuries/physiopathology , Cerebrovascular Disorders/pathology , Nerve Growth Factors/blood , Neuroglia/metabolism , Postmortem Changes , S100 Calcium Binding Protein beta Subunit , S100 Proteins/blood , Severity of Illness Index , Time Factorsالملخص
In the modern biotechnology era,the important disease genetic resources become the most dependent factor.We expound the actuality of the collection,storage and use of the important disease genetic resources in China,and discuss the problems and challenges we meet.So the chief mission we should do is establishing the database and collecting,storing and using these valuable resources under standard procedure with aim,plan and organization.