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ObjectiveTo explore the correlation between blood stasis constitution （BSC） and diseases based on constitution literature involving “constitution-diseases correlation”. MethodsA comprehensive search was conducted on six Chinese and English electronic databases including CNKI， Wanfang， VIP， SinoMed， PubMed and Embase to find all clinical researches on the correlation between constitution and diseases using the Classification and Identification of Chinese Medicine Constitution standard from April 1st， 2009 to December 31st， 2022， and the participants of the research were BSC related. By analyzing the characteristics of the literature， such as authors， publication institutes， participants， and results， the disease with the highest proportion of BSC distribution or BSC as their risk factors or protective factors were summarized to explore the correlation between BSC and diseases. ResultsTotally 135 clinical studies on diseases highly related to BSC were included， with a total sample size of 71 172 cases.There were 27 keywords in the articles appeared more than 3 times， including the elderly， lumbar disc herniation， coronary heart disease， cardiovascular disease， and endometriosis. In the author's clustering， included studis were mainly from Shenzhen Hospital of Beijing University of Chinese Medicine， Second Affiliated Hospital of Guangzhou University of Chinese Medicine， and Wenzhou Central Hospital. In terms of blood stasis related diseases， 81 studies showed that BSC was the most common type of constitution in the study population， involving 48 disease or morbid states. The diseases and median proportions of BSC with reported literature ≥3 included coronary heart disease （28.8%）， endometriosis （31.3%）， neurocognitive impairment （26.4%）， lumbar disc herniation （26.0%）， ischemic stroke （25.0%）， adenomyosis （34.7%）， and endometrial polyps （25.0%）. Fifty-eight studies found that BSC was a risk factor for disease occurrence，and these diseases reported more than 3 times included hypertension （median OR = 2.956）， type 2 diabetes （median OR = 3.436），osteoporosis （median OR = 5.171）， sudden deafness （median OR = 3.827） and endometriosis （median OR = 5.412）. One study indicated BSC as the protective factor of lateral growth tumor of large intestine （median OR = 0.161）. ConclusionBSC is closely related to circulatory system diseases， urogenital system diseases， and musculoskeletal system diseases.
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Objetivo:analisar relações existentes entre proposta conceitual do movimento das Universidades Promotoras da Saúde e projetos de extensão de uma universidade, sob perspectiva de uma análise documental. Método:estudo de caso documental com análise qualitativa descritiva realizado com base nos documentos de registros extensionistas de uma universidade pública do estado do Rio de Janeiro. A análise deste estudo teve como aporte teórico a proposta conceitual do movimento das Universidades Promotoras da Saúde. Resultados:identificaram-se 11 projetos que atenderam aos critérios de inclusão e, foram distribuídos em cinco categorias. Conclusão:é necessário vincular a promoção da saúde ao projeto político pedagógico da Universidade, possibilitando a valorização das experiências formativas, no âmbito das histórias de vida dos sujeitos e das suas vivências comunitárias e no âmbito da política institucional. Entretanto, tais iniciativas ainda são escassas, gerando um hiato entre expectativa e realidade.
Objective:to analyze existing relationships between the conceptual proposal of the health promoting universities movement and university extension projects, from the perspective of a documental analysis. Method:documental case study with descriptive qualitative analysis based on records documents from a public university in the state of Rio de Janeiro. The analysis of this study had as theoretical contribution the conceptual proposal of the Health Promoting Universities movement. Results: 11projects thatmet the inclusion criteria were identified and distributed into five categories. Conclusion:it is necessary to link health promotion to the University's political pedagogical project, enabling the valorization of formative experiences, within the scope of the subjects' life stories, their community experiences and within the scope of institutional policy. However, such initiatives are still scarce, creating a gap between expectations and reality.
Objetivo:analizar relaciones existentes entre propuesta conceptual del movimiento Universidades Promotoras de Salud y los proyectos de extensión de una universidad, desde la perspectiva de un análisis documental. Método:estudio de caso documental con análisis cualitativo descriptivo basado en registros extensión de una universidad pública del estado de Río de Janeiro. El análisis de este estudio tuvo como soporte teórico del movimiento de Universidades Promotoras de Salud. Resultados:se identificaron once proyectos que cumplieron con los criterios de inclusión y se distribuyeron en cinco categorías. Conclusión:es necesario vincular la promoción de la salud al proyecto político pedagógico de la Universidad, posibilitando la valorización de las experiencias formativas, en el ámbito de las historias de vida de los sujetos y de sus experiencias comunitarias y en elámbito de la política institucional. Sin embargo, tales iniciativas aún son escasas, creando una brecha entre las expectativas y la realidad.
الموضوعات
Community-Institutional Relations , Universities , Public Health , Professional Trainingالملخص
@#Objective To investigate the effect of caloric restriction(CR)on myocardial ischemia/reperfusion injury(MI/RI)in mice and its mechanism.Methods C57 mice were randomly divided into normal diet group(AL group,free feeding)and CR group(diet decreased by 10% every 2 weeks)for 8 weeks and monitored for weight changes.Each group was divided into sham operation group and MI/RI group,total 4 groups,AL + Sham group,AL + I/R group,CR + Sham group and CR + I/R group).The left anterior descending coronary artery was ligated for 30 minutes and then reperfused for 24 hours in mice of MI/RI group and mice in Sham group were only threaded but not ligated.The mice were determined for myocardial ischemia and infarct size by Evans blue/TTC staining,observed for the pathology of myocardium by HE staining,determined for the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD)and the contents of creatine kinase-MB(CK-MB)and malondialdehvde(MDA)in myocardium by the corresponding kits,determined for serum levels of IL-1β and IL-18 by ELISA and detected for the expression of pyroptosis-associated proteins in myocardium by Western blot.Results After 8weeks,the weights of mice in CR group[(24.54 ± 0.41)g]were significantly lower than those in AL group[(31.46 ±0.25)g](t = 14.34,P<0.05).Compared with those in AL + I/R group,the area of myocardial ischemia in CR + I/R group showed no significant difference(t = 0.783 0,P>0.05),while the area of myocardial infarction decreased significantly(t = 7.250,P<0.01);The myocardial arrangement was relatively neat,and the degree of pathological changes was obviously reduced;LDH activity,CK-MB and MDA contents decreased significantly(t = 4.331,2.875 and 5.343 respectively,each P<0.05),while SOD activity increased significantly(t = 4.211,P<0.05);Serum levels of IL-1β and IL-18 decreased significantly(t = 3.375 and 4.266 respectively,each P<0.05);The expression levels of nod-like receptor protein 3(NLRP3),gasdermin D(GSDMD),apoptosis-associated speckle-like protein(ASC)and caspase-1 significantly decreased(t = 3.412,3.420,3.480 and 2.585 respectively,each P<0.05).Conclusion CR alleviated MI/RI in mice,and its mechanism was related to the inhibition of cardiac pyroptosis.
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In the era of artificial intelligence based on big data， data acquisition， storage and processing are more convenient， which provides a guarantee for accelerating the development of traditional Chinese medicine （TCM）， but it has not yet achieved organic integration with TCM theory. Based on preliminary research on the supramolecular "Qi chromatography" theory of TCM， combined with the current development trend of artificial intelligence， this paper analyzed the biological intelligence attribute of the function of TCM supramolecular "imprinting template"， in order to provide reference for the development of TCM drug innovation. Both the human body and Chinese materia medica are giant complex supramolecular bodies evolved from natural organisms. According to the "imprinting template"， the "social molecules" are controlled step by step to form the meridians and viscera. The interaction produces the original theory of TCM， in which the self-recognition， self-assembly， self-organization and self-replication of the "imprinting template" reflect the "intelligence" function attributes：the human body uses the "imprinting template" to self-identify and sense the ingredients of TCM， and store the memory information database in the meridian and collateral organs in the form of "imprinting template"， and then pass the "imprinting template". The comparison， analysis， and judgment of imprinting templates guide the self-assembly， self-organization and self-replication among "molecular society"， synthesize biological machines， produce biological functions， repair or strengthen biological supramolecular bodies， and present the most basic "intelligence" attribute. This suggests that the theory of theory-method-prescription-medicine of TCM is the weak embodiment of biological "intelligence"， while the human brain function is the strong embodiment of biological "intelligence". Since the intelligent function of supramolecular "imprinting template" runs through the natural world， artificial intelligence that can characterize the strong "intelligence" form of the human brain will also be integrated into all aspects of the natural world， suggesting the development direction of "intelligence" functionalization of drug innovation mode.
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Objective To explore the possibility of rat adipose-derived stem cells (ADSCs) to differentiate into oligodendrocyte precursor cells(OPCs) and find an effective way to treat demyelinating disease. Methods ADSCs from the inguinal region of SD rats were isolated, digested with collagenase type I and trypsin, collagenase type I digestion method as control, counted and compared; Cultured in vitro and observed the growth characteristics. After ADSCs subcultured 3 times of passages, CD29, CD90 and CD45 were detected by flow cytometry; After differentiation into adipocyte, the cells were identified by the staining of oil red 0; After differentiation into OPCs by stem cell differentiation medium and OPCs induced differentiation medium, the expression of a-N-acetylneuraminic acid a-2, 8-sialyltransferase I (A2B5) and NG2 was detected by immunofluorescent staining. Results The number of ADSCs in the combined enzyme group was higher than the collagenase type 1 group (P < 0 . 05, re = 7); ADSCs grew in a long shuttle type and their morphology tended to be stable after passage. The surface marker CD29, CD90 were positive, and CD45 was negative. After adipogenic induction, oil red 0 staining showed red lipid droplets of varying sizes in the cells. After OPCs induction, immunofluorescence detection showed that positive reaction of cell surface fluorescence was seen with antibody to A2B5 and NG2,(87. 03±0. 94)% expressed A2B5, (90. 07±0. 96) % expressed NG2. After cultured for 3 days, immunof'luorescence detection showed that positive reaction of cell surface fluorescence was seen with antibody to myelin basic protein (MBP). Conclusion ADSCs are obtained by combined enzyme digestion and the cells are much more than collagenase alone and can be induced to OPCs in vitro.
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The application of modern scientific theories and technologies to explore the mechanism of Chinese medicine and its compounds is one of the key issues in realizing the modernization of traditional Chinese medicine （TCM） research. Chinese medicine and its compounds produce comprehensive pharmacodynamics through multiple components acting on multiple targets， the core of clarifying the mechanism is to solve the key scientific problems of static correlation and dynamic integration verification between the components and the target network topology. At present， the effective method to clarify the mechanism of Chinese medicine and its compounds is to statically correlate the topological network of in vitro components and targets through network pharmacology. Although there are also component-target verification studies， they often learn from research idea of single component-single target， it is urgent to establish a quantitative integration and overall verification method that conforms to the characteristics of TCM. According to supramolecular Qi chromatography theory of TCM， the microscopic mechanism of interaction between Chinese medicine and human body is actually the two supramolecular host and object groups （the active ingredient group of Chinese medicine and the target group of human body） based on imprinted template， which shows the macroscopic properties and pharmacodynamics. Based on this， the author proposes to use supramolecular Qi chromatography theory as the guidance， combined with supramolecular chemistry， network dynamics， quantitative pharmacology and other methods to quantitatively integrate and verify the compositions and the target groups with imprinted template as the core predicted by network pharmacology， looking for the optimal quality markers， greatly reducing the difficulty of multi-component-multi-target experimental verification of Chinese medicine and its compounds.
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Objective:To construct the targeting evaluation method of traditional Chinese medicine （TCM） preparations based on supramolecular Qi chromatography theory， and to study the liver targeting effect of Bupleuri Radix on Pien Tze Huang. Method:The molecular connectivity index （MCI） was used to analyze the characteristics of imprinted template and liver targeting tendency of TCM mainly attributed to liver meridian and components of Pien Tze Huang， and combined with target dynamics and total statistical moment principle， aimed at the independent action characteristics of multi-component imprinted template of TCM， a method for evaluating the targeting of TCM preparations was established. Hepatoma rats in Pien Tze Huang group， Bupleuri Radix<italic> </italic>group， Pien Tze Huang+Bupleuri Radix group and blank group were tested and verified. Result:After the average value of MCI of TCM mainly attributed to liver meridian was deducted， the MCI similarity between Pien Tze Huang group and Bupleuri Radix group was 0.376 8， Pien Tze Huang+Bupleuri Radix group and Bupleuri Radix group was 0.988 2， so it was predicted that Bupleuri Radix could enhance the liver targeting of Pien Tze Huang. A system for evaluating the targeting of TCM compounds was established， including relative total uptake efficiency （RUE<sub>T</sub>）， relative total concentration （RC<sub>T</sub>）， relative imprinted tendency （RIT<sub>T</sub>） and relative imprinted variance （RIV<sub>T</sub>）. The RUE<sub>T</sub> and RC<sub>T</sub> of liver were the highest in all tissues （RUE<sub>T</sub>=1.88>1，RC<sub>T</sub><italic>=</italic>2.30>1）， and the corresponding values of other tissues were all <1， indicating that Pien Tze Huang combined with Bupleuri Radix could increase its distribution in liver and enhance liver targeting. Except for plasma， the RIT<sub>T</sub> and RIV<sub>T</sub> of other tissues fluctuated around 1.0， indicating that targeted modification did not change imprinted tendency of Pien Tze Huang and had no significant effect on the types of components. Conclusion:Under the guidance of supramolecular Qi chromatography theory， a targeting evaluation parameter system can be established to characterize the multi-component imprinted effect of TCM preparations by MCI and total statistical moment parameters， so as to realize the evaluation of targeting of TCM preparations. The addition of Bupleuri Radix can increase the liver targeting of Pien Tze Huang.
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Objetivo refletir sobre as implicações do trabalho em Home Office no período da pandemia de COVID-19 na saúde dos indivíduos, sob a perspectiva da Teoria da Adaptação desenvolvida por Callista Roy. Método estudo reflexivo baseado na aplicação da Teoria da Adaptação desenvolvida por Callista Roy relacionada às modificações do processo de trabalho impostas pela crise sanitária da pandemia de COVID-19, com ênfase no Home Office. Resultados a Teoria da Adaptação de Callista Roy possui quatro modos adaptativos: fisiológico, autoconceito, desempenho de papel e interdependência. É possível verificar a interlocução de todas essas dimensões no trabalho em Home Office imposto pelo contexto da pandemia. Conclusão a Teoria de Callista Roy subsidia as discussões sobre a possibilidade de adaptação neste novo contexto, seja de maneira pontual ou mediante transformações no processo de trabalho em longo prazo, superando limitações do indivíduo e descobrindo maneiras de se fazer e ser no campo do trabalho.
Objetivo reflexionar sobre las implicaciones del trabajo Home Office en el período de la pandemia de COVID-19 sobre la salud de las personas, desde la perspectiva de la Teoría de la Adaptación desarrollada por Callista Roy. Método estudio reflexivo basado en la aplicación de la Teoría de la Adaptación desarrollada por Callista Roy relacionado con cambios en el proceso de trabajo impuestos por la crisis de salud de la pandemia de COVID-19, con énfasis en el Home Office. Resultados La Teoría de la Adaptación de Callista Roy tiene cuatro modos adaptativos: fisiológico, autoconcepto, ejecución del papel e interdependencia. Puede verificarse la interlocución de todas estas dimensiones en el trabajo Home Office impuesto por el contexto de la pandemia. Conclusión la Teoría de Callista Roy apoya discusiones sobre la posibilidad de adaptación en este nuevo contexto, ya sea de manera puntual o a través de transformaciones en el proceso de trabajo a largo plazo, superando las limitaciones del individuo y descubriendo formas de hacer y estar en el campo del trabajo.
Objective to reflect on the implications of Home Office work in the covid-19 pandemic period on individuals' health, from the perspective of the Adaptation Theory developed by Callista Roy. Method reflective study based on the application of the Adaptation Theory developed by Callista Roy related to changes in the work process imposed by the health crisis of the COVID-19 pandemic, with emphasis on the Home Office. Results Callista Roy's Adaptation Theory has four adaptive modes: physiological, self-concept, role performance and interdependence. There is the interlocution of all these dimensions at Home Office work imposed by the pandemic context. Conclusion Callista Roy's Theory supports discussions about the possibility of adaptation in this new context, either in a specific way or through transformations in the long-term work process, overcoming limitations of the individual and discovering ways to do and be in the work field.
الموضوعات
Humans , Nursing Theory , Occupational Health , Pandemics , Nursing , Coronavirus Infections , Qualitative Researchالملخص
Objective Apolipoprotein E (APOE) is mainly involved in lipid metabolism and cholesterol transport, which is important for health. To establish a pyrosequencing based method for detection of 112T>C and 158C>T single nucleotide polymorphisms (SNPs) in Apolipoprotein E gene. Methods Three amplification systems including Taq enzyme buffer from TaKaRa company (T buffer), La Taq enzyme buffer specified for G-C rich regions (L buffer) and Trans Taq enzyme buffer from TransGen company (TT buffer) were chosen to optimize PCR system and temperature by annealing at 60 °C and gradient annealing from 62 to 68 °C respectively. The specificity of this method was evaluated by comparing its results with those of Sanger sequencing. The sensitivity of this method was evaluated by gradient diluting human genomic DNA as detection template. Results According to the concentration and specificity of the products, the optimum condition was L buffer with 60℃ annealing programs. Pyrosequencing results of 20 samples were completely consistent with those of Sanger sequencing. The sensitivity of this method could be as low as 0.16 ng genomic DNA. Conclusion A method based on pyrosequencing detecting 112 and 158 polymorphisms in APOE gene was established, which can be applied in clinical personalized medicine.
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The present study was designed to evaluate protective activity of an ethanol extract of the stems of Schisandra chinensis (SCE) and explore its possible molecular mechanisms on acetaminophen (APAP) induced hepatotoxicity in a mouse model. The results of HPLC analysis showed that the main components of SCE included schisandrol A, schisandrol B, deoxyschisandrin, schisandrin B, and schisandrin C and their contents were 5.83, 7.11, 2.13, 4.86, 0.42 mg·g, respectively. SCE extract was given for 7 consecutive days before a single hepatotoxic dose of APAP (250 mg·kg) was injected to mice. Our results showed that SCE pretreatment ameliorated liver dysfunction and oxidative stress, which was evidenced by significant decreases in aspartate transaminase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) contents and elevations in reduced glutathione (GSH) and superoxide dismutase (SOD) levels. These findings were associated with the result that the SCE pretreatment significantly decreased expression levels of 4-hydroxynonenal (4-HNE) and 3-nitrotyrosine (3-NT). SCE also significantly decreased the expression levels of Bax, mitogen- activated protein kinase (MAPK), and cleaved caspase-3 by APAP exposure. Furthermore, supplementation with SCE suppressed the expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), suggesting alleviation of inflammatory response. In summary, these findings from the present study clearly demonstrated that SCE exerted significant alleviation in APAP-induced oxidative stress, inflammation and apoptosis mainly via regulating MAPK and caspase-3 signaling pathways.
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Animals , Humans , Male , Mice , Acetaminophen , Alanine Transaminase , Metabolism , Apoptosis , Aspartate Aminotransferases , Metabolism , Caspase 3 , Genetics , Metabolism , Chemical and Drug Induced Liver Injury , Genetics , Metabolism , Drugs, Chinese Herbal , Chemistry , Glutathione , Metabolism , Liver , Metabolism , Malondialdehyde , Metabolism , Mice, Inbred ICR , Mitogen-Activated Protein Kinases , Chemistry , Genetics , Metabolism , Oxidative Stress , Schisandra , Chemistry , Signal Transductionالملخص
OBJECTIVE: To study the effect of extract from the stems of Schisandra chinensis(SCE) on high-fat diet (HFD) induced obesity in mice. METHODS: The model of HFD induced obese model of C57BL/6J mice for 8 weeks was established. The contents of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TCHO), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol(HDL-C) and hepatic triglyceride (TG), total cholesterol (T-CHO), glutathione (GSH) and malondialdehyde(MDA) were measured. The pathological changes with H&E staining and oil red O was observed for investigating the effect of SCE in the obese mice. In addition, the assay of CYP2E1, 4-HNE using immunofluorescence staining and the expression of iNOS and COX-2 protein with Western blot analyzing was measured to explore the effect of SCE on lipid peroxidation and inflammatory response. RESULTS: Compared with the HFD group, low and high dose groups of SCE significantly reduced the serum AST, ALT, TG, T-CHO, LDL-C and hepatic TG, TC and MDA levels, elevated the serum HDL-C and hepatic GSH levels and improved liver tissue steatosis, inflammatory infiltration and accumulation of lipid droplets, oxidative stress and inflammatory response. CONCLUSION: SCE may improve lipid metabolism in HFD induced obese mice by inhibiting oxidative stress and inflammatory response.
الملخص
The present study was designed to evaluate protective activity of an ethanol extract of the stems of Schisandra chinensis (SCE) and explore its possible molecular mechanisms on acetaminophen (APAP) induced hepatotoxicity in a mouse model. The results of HPLC analysis showed that the main components of SCE included schisandrol A, schisandrol B, deoxyschisandrin, schisandrin B, and schisandrin C and their contents were 5.83, 7.11, 2.13, 4.86, 0.42 mg·g, respectively. SCE extract was given for 7 consecutive days before a single hepatotoxic dose of APAP (250 mg·kg) was injected to mice. Our results showed that SCE pretreatment ameliorated liver dysfunction and oxidative stress, which was evidenced by significant decreases in aspartate transaminase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) contents and elevations in reduced glutathione (GSH) and superoxide dismutase (SOD) levels. These findings were associated with the result that the SCE pretreatment significantly decreased expression levels of 4-hydroxynonenal (4-HNE) and 3-nitrotyrosine (3-NT). SCE also significantly decreased the expression levels of Bax, mitogen- activated protein kinase (MAPK), and cleaved caspase-3 by APAP exposure. Furthermore, supplementation with SCE suppressed the expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), suggesting alleviation of inflammatory response. In summary, these findings from the present study clearly demonstrated that SCE exerted significant alleviation in APAP-induced oxidative stress, inflammation and apoptosis mainly via regulating MAPK and caspase-3 signaling pathways.
الموضوعات
Animals , Humans , Male , Mice , Acetaminophen , Alanine Transaminase , Metabolism , Apoptosis , Aspartate Aminotransferases , Metabolism , Caspase 3 , Genetics , Metabolism , Chemical and Drug Induced Liver Injury , Genetics , Metabolism , Drugs, Chinese Herbal , Chemistry , Glutathione , Metabolism , Liver , Metabolism , Malondialdehyde , Metabolism , Mice, Inbred ICR , Mitogen-Activated Protein Kinases , Chemistry , Genetics , Metabolism , Oxidative Stress , Schisandra , Chemistry , Signal Transductionالملخص
OBJECTIVE@#To observe the effect of curcumin on expressions of nuclear transcription factor-kappa Bp65 (NF-κBp65), TNF-α and IL-8 in placental tissue of premature birth of infected mice induced by lipopolysaccharide (LPS).@*METHODS@#A total of 60 C57BL/6 mice pregnant with 15 d were collected and randomly divided into control group, model group, treatment group and preventative group. LPS was repeatedly injected in abdominal cavity to construct infected premature birth model, while mice of control group were given with 100 mg/kg of vitamin C through abdominal cavity injection and mice of treatment group and preventative group were given curcumin of 100 mg/kg through abdominal cavity injection after modeling operation and before 1 d of modeling operation, respectively. A total of 5 mice of four groups respectively were executed by cervical dislocation after 6 h, 12 h and 24 h after constructing model. Placental tissues were collected and the immunohistochemical method SABC of immunologic tissue was used to detect the expression of NF-κBp65, TNF-α and IL-8 and peripheral blood of executed mice after 24 h was collected to detect the concentrations of IL-8, malondialdehyde (MDA) and superoxide dismutase (SOD), meanwhile live birth rate of four groups was contrasted.@*RESULTS@#Staining intensity of NF-κBp65, TNF-α and IL-8 in placental tissue of treatment group and preventative group was significantly higher than control group but lower than model group (P < 0.05). Level of serum IL-8 and MDA of control group was significantly lower than the other three groups (P < 0.05) and level of blood of SOD in model group was significantly lower than control group (P < 0.05). Levels of serum IL-8 and MDA of treatment group and preventative group were significantly lower than model group (P < 0.05) while level of SOD was significantly higher than model group (P < 0.05). Live birth rate of treatment group and preventative group was significantly higher than model group (P < 0.05).@*CONCLUSIONS@#Curcumin can effectively prevent the active pathway of NF-κB in pregnant tissue of premature birth of infected mice, reduce the expression of TNF-α and IL-8 and relieve the damage of lipid peroxide of oxidative stress of LPS on mother-fetus and further to achieve the objective of preventing and curing premature birth induced with infection.
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Objective To observe the effect of curcumin on expressions of nuclear transcription factor-kappa Bp65 (NF-κBp65), TNF-α and IL-8 in placental tissue of premature birth of infected mice induced by lipopolysaccharide (LPS). Methods A total of 60 C57BL/6 mice pregnant with 15 d were collected and randomly divided into control group, model group, treatment group and preventative group. LPS was repeatedly injected in abdominal cavity to construct infected premature birth model, while mice of control group were given with 100 mg/kg of vitamin C through abdominal cavity injection and mice of treatment group and preventative group were given curcumin of 100 mg/kg through abdominal cavity injection after modeling operation and before 1 d of modeling operation, respectively. A total of 5 mice of four groups respectively were executed by cervical dislocation after 6 h, 12 h and 24 h after constructing model. Placental tissues were collected and the immunohistochemical method SABC of immunologic tissue was used to detect the expression of NF-κBp65, TNF-α and IL-8 and peripheral blood of executed mice after 24 h was collected to detect the concentrations of IL-8, malondialdehyde (MDA) and superoxide dismutase (SOD), meanwhile live birth rate of four groups was contrasted. Results Staining intensity of NF-κBp65, TNF-α and IL-8 in placental tissue of treatment group and preventative group was significantly higher than control group but lower than model group (P < 0.05). Level of serum IL-8 and MDA of control group was significantly lower than the other three groups (P < 0.05) and level of blood of SOD in model group was significantly lower than control group (P < 0.05). Levels of serum IL-8 and MDA of treatment group and preventative group were significantly lower than model group (P < 0.05) while level of SOD was significantly higher than model group (P < 0.05). Live birth rate of treatment group and preventative group was significantly higher than model group (P < 0.05). Conclusions Curcumin can effectively prevent the active pathway of NF-κB in pregnant tissue of premature birth of infected mice, reduce the expression of TNF-α and IL-8 and relieve the damage of lipid peroxide of oxidative stress of LPS on mother-fetus and further to achieve the objective of preventing and curing premature birth induced with infection.
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Objective To investigate the relationship between nasal colonization of Staphylococcus aureus(SA) and nosocomial infection in intensive care unit(ICU), and observe the therapeutic effect of Anerdian III in nasal decolonizaion. Methods Bacterial cultures were made by means of nasal swabs among inpatients whom the occurrence of nosocomial infection were observed.Patients with SA colonization were randomly divided into two groups:control and treatment.Control group were given regular treatment, and treatment group were administered Anerdian III in addition to regular treatment.Then the clearance rate of SA and the occurrence of nosocomial infection of two groups were observed. Results A total of 751 patients were enrolled, of whom 108(14.4%) were with nosocomial infection and 85(11.3%) with SA nasal colonization. Methicillin resistant Staphylococcus aureus ( MRSA ) was detected in 33 patients (4.4%).The nosocomial infection rate of patients with MRSA colonization was 51.5%, which was significantly higher than those in patients with other bacterial colonization(P<0.05).The SA clearance rate in treatment group was significantly higher than that in control group(81.4% vs.42.8%,P<0.05).The nosocomial infection rate in treatment group was significantly lower than that in control group ( 16 .3% vs. 40.5%,P <0.05).After decolonization treatment,the nosocomial infection rate of patients with MRSA colonization was significantly lower than that in control group(25.0% vs.76.5%,P <0.05). Conclusion The incidence rate of nosocomial infection in patients with MRSA nasal colonization is markedly increased in ICU, and the decolonization treatment by Anerdian III increases the clearance rate of nasal SA and decreases the incidence rate of nosocomial infection.
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Objective] To discuss comprehensive intervention effect on the control of hospital in-fections in the intensive care unit ( ICU ) of a hospital by monitoring Staphylococcus aureus infections and their drug resistance . [ Methods] Comparative analysis was done retrospectively in separation results of Staphylococcus aureus between 2011 and 2012 in ICU patients of a hospital . [ Results] Between 2011 and 2012, there was no obvious difference found in relevance ratio of Staphylococcus aureus(P>0.05), but that of methicillin resistant Staphylococcus aureus was on the decline significantly (P<0.05).The drug re-sistance rates of Staphylococcus aureus to oxacillin, ciprofloxacin, levofloxacin were on the decline signifi-cantly(P <0.05).The drug susceptibility rates of Staphylococcus aureus to vancomycin, teicoplanin, linezolid , nitrofurantoin and primaquine slave tianeptine/dafoe tianeptine were the highest , reaching up to 100.00%. [ Conclusion] By comprehensive intervention , Staphylococcus aureus infections in ICU have been improved and drug resistance rates on the decline as a whole .
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<p><b>OBJECTIVE</b>Six1 and Six4 are expressed in several tumors, and associated with tumor progress and poor prognosis. The aim of this study was to investigate the expression of Six1 and Six4 in esophageal squamous cell carcinoma (ESCC), and to evaluate their correlation with the clinicopathological factors and prognosis.</p><p><b>METHODS</b>Tissue microarray technology and immunohistochemical method (EnVision) were used to detect the expression of Six1 and Six4 in the tumor tissues and corresponding adjacent normal epithelium of esophagus from 292 ESCC patients.</p><p><b>RESULTS</b>Among the 292 ESCC patients, the positive rates of Six1 and Six4 protein expression in tumor tissues were 72.9% (213/292) and 56.2% (164/292), respectively, significantly higher than the expression rate of 33.2% (97/292) and 32.5% (95/292) in adjacent normal epithelium of esophagus (P < 0.05). Chi square test showed that the expression of Six1 protein was related to tumor size, depth of tumor invasion and patient survival status; higher Six4 protein expression level was related to poor differentiation and increased depth of invasion. Single factor Log-rank analysis revealed that gender, TNM stage, Six1 protein expression level were related to the overall survival of ESCC patients (P < 0.05), while the five-year survival rate was significantly higher in the Six1-negative group than the Six1-positive group [51.9% (41/79) vs. 43.7% (93/213)]. Multi-factor Cox proportional risk model analysis showed that TNM stage and positive expression of Six1 were independent prognostic factors for ESCC patients (P < 0.05).</p><p><b>CONCLUSIONS</b>Six1 and Six4 are highly expressed in ESCC. Their expression levels are closely related to the progress and prognosis of ESCC. Over-expression of Six1 is related to poor prognosis in ESCC patients. Thus, Six1 could be used as an important prognostic indicator for ESCC patients.</p>
الموضوعات
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , General Surgery , Esophageal Neoplasms , Metabolism , Pathology , General Surgery , Follow-Up Studies , Homeodomain Proteins , Metabolism , Lymphatic Metastasis , Neoplasm Invasiveness , Neoplasm Staging , Prognosis , Proportional Hazards Models , Risk Factors , Survival Rate , Trans-Activators , Metabolism , Tumor Burdenالملخص
Objective To investigate the cause of differences in confluent growth between hepatic(L02) and hepatoma carcinoma(HCCLM3) cells by comparing responses of the two cells to different substrate stiffness (0.5, 4 kPa and glass). MethodsThe real-time photomicrography, immunofluorescence staining, flow cytometry, and Western Blotting techniques were respectively employed to observe the morphological characteristics, the cytoskeleton conformation and the distribution of E-cad of confluent L02 and HCCLM3 cells on different substrates, and test the changes in expression of E-cad, Integrinβ1 and p-Src. Results (1) Confluent L02 cells displayed a round or cubic shape, while HCCLM3 cells showed a polygon shape. The morphology of HCCLM3 cells were spread and polarized more obviously than that of L02 cells. With the increase of substrate stiffness, the variation of L02 cells with time was smaller than that of HCCLM3 cells. (2) The cytoskeleton of confluent L02 cells showed a ring-like conformation under the cortex, and E-cad was located at the cell-cell contact sites. However, the ring-like cytoskeleton of HCCLM3 cells was incomplete and distributed radially along the basement, while E-cad was dispersed in cytoplasm. (3) As the substrate stiffness increased, expression of E-cadherin in both L02 and HCCLM3 cells was significantly decreased (P<0.01), while the level of p-Src and integrinβ1 was increased significantly, with greater changes in HCCLM3 cells than in L02 cells. Conclusions The assembling of cortical ring-like cytoskeleton was positively correlated with the location of E-cad at the cell-cell contact sites. The substrate stiffness showed a more obvious impact on the balanced regulation between cadherin and integrin mediated adhesion system of hepatocarcinoma cells than that of hepatic cells.
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Objective To investigate the effects of substrate stiffness on the adhesion, spreading and migration of hepatocellular carcinoma cells as well as the regulation of cytoskeleton assembly and integrinβ1 expression, and to explore the role of substrate mechanical properties in the metastasis of hepatocellular carcinoma cells. Methods The polyarcylamide gel with different stiffness was achieved by varying the relative ratio of acrylamide to bis acrylamide. The substrate surface was cross linked with extracellular matrix molecules for cell adhesion. The adhesion, spreading and migration of hepatocellular carcinoma cells on substrates with different stiffness were recorded by phase contrast microscope and made quantitative analysis by Image J software. The cytoskeleton assembly on substrates with different stiffness was detected by immunofluorences assay, and the expression of integrinβ1on different substrates was measured by flow cytometer. Results The rigid substrate enhanced the adhesion and spreading of hepatocellular carcinoma cells in shortened time. Neither the soft (1.1 kPa) nor over rigid (glass) substrate facilitated the migration of hepatocellular carcinoma cells, and the maximum migration velocity was found on the substrate with moderate stiffness（10.7 kPa）. The rigid substrate could promote cytoskeleton assembly and integrinβ1 expression. Conclusions The effects of substrate stiffness on adhesion, spreading and migration of hepatocellular carcinoma cells are regulated by the cytoskeleton assembly and integrin expression.
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<p><b>OBJECTIVE</b>To study the mechanism of the effect of low-frequency rotary constant magnetic field on high-fat and high-protein diet-induced fatty liver in rats.</p><p><b>METHODS</b>Fatty liver model was established in SD rats by feeding on a high-fat and high-protein diet daily. The enzyme activity changes in the serum and liver homogenate were detected at 10, 14, and 18 weeks, and the pathological changes of the liver were observed with optical and electron microscopy.</p><p><b>RESULTS</b>In magnetic field intervention group, the concentration of alanine aminotransferase and aspartate transaminase were significantly decreased, and the activity of lipoprotein lipase, hepatic lipase, superoxide dismutase and the concentration of malondialdehyde in the liver homogenate were significantly increased. Under optical microscope and electron microscope, the rats in the model group showed diffusive adipose degeneration in the hepatic cells with large lipid droplets, which became large vacuoles after fat extraction, indicating fatty necrosis. In magnetic field intervention group, remarkably smaller lipid droplets and lessened hepatic cell adipose degeneration were observed.</p><p><b>CONCLUSION</b>Low-frequency rotary constant magnetic field has beneficial effect on fat metabolism, leading to reduced lipid peroxidation and structural recovery of the degenerated hepatic cells.</p>