Effect evaluation of micro-video assisted physiological theory teaching based on functional experiments / 中华医学教育探索杂志

Objective:To investigate the implementation effect of micro-video assisted physiological theory teaching based on functional experiments.Methods:There were 140 clinical undergraduates (control group) from Class 1 and Class 2, and 123 clinical undergraduates (experimental group) from Class3 and Class 4 of Batch 2017 in our university who were involved in this study. The control group adopted traditional teaching method, the experimental group adopted micro-videos to assist traditional teaching in the teaching of selected chapters, and these micro-videos were collected from the recording and editing of functional experiments. After the course, questionnaire survey in terms of course design, implementation and effect, as well as final exanimation performance analysis were conducted to evaluate the teaching effect. SPSS 17.0 was used for t test. Results:The final examination scores of the experimental group were significantly higher than those of the control group [(81.02±9.64) vs. (73.41±11.39)], with significant differences ( t=-5.805, P<0.001). Among them, the scores of Chapter 2 of the two groups were [(8.07±0.94) vs. (6.14±1.05), t=-15.616, P<0.001)], the scores of Chapter 4 were [(16.16±1.79) vs. (10.90±2.23), t=-20.903, P<0.001)], and the scores of Chapter 6 were [(6.04±0.53) vs. (5.82±0.78), t=-2.638, P=0.009)], all with significant differences. 100% of questionnaires were recovered, and more than 90% of students were interested in this teaching method which could strengthen their understanding of the key and difficulties in physiology and was also helpful to cultivate their ability of induction and summarization. Conclusion:Micro-videos based on functional experiments assisted teaching can improve the teaching effect of physiology, and it's worth popularizing.

Metabolic differences of tacrolimus between patients with combined liver and kidney transplantation and patients with single liver or single kidney transplantation / 中国中西医结合急救杂志

Objective To investigate the metabolic differences of Tacrolimus in different transplant recipients. Methods A retrospective study was conducted, 30 patients of the organ transplant admitted to organ transplantation center of General Hospital of Chinese People's Armed Police Forces from January 2002 to August 2012 were enrolled, and they were divided into combined liver and kidney transplant (SLK) group, single liver transplant group and single renal transplant group, 10 cases in each group. The SLK group and the simple liver transplantation group were given the same drug regimen, methylprednisolone injection was given during operation, and tacrolimus+ mycophenolate mofetil+ prednisone triple immunosuppressive therapy was taken after operation, on the first day after operation, the initial dose of tacrolimus was given 0.06 mg·kg-1·d-1 divided into 2 times taken orally, and on the third day after operation, the concentration of tacrolimus was detected; after operation group for 2 - 4 days, single renal transplantation group was given tacrolimus the initial dose of tacrolimus 0.15 mg·kg-1·d-1 was divided into 2 parts for twice oral administration, after 2 - 3 days of the above treatment, monitoring the concentration of tacrolimus began. One month after transplantation, the metabolic differences of tacrolimus among the three groups were compared.Results One month after operation, the oral tacrolimus doses (μg·kg-1·d-1: 74.78±32.65 vs. 80.62±24.02, 85.58±16.78) and the monitored blood drug concentration (μg/L: 6.64±2.73 vs. 7.50±3.08, 7.46±3.20) in SLK group were lower than either the single liver transplantation group or single renal transplantation group, but the comparisons among the three groups, there were no statistically significant differences(allP > 0.05).Conclusions In SLK group, the protective effect of transplanted liver on transplanted kidney may be related to the length of postoperative time. Liver transplantation performed within post-operative one month has no protective effect on the transplanted kidney.

Construction of colorectal cancer cell line stably expressing mir-101 and identification of the target gene of mir-101 / 南方医科大学学报

<p><b>OBJECTIVE</b>To construct a colorectal cancer cell line stably expressing mir-101 and identify the target gene of mir-101.</p><p><b>METHODS</b>Quantitative real-time PCR was used to detect mir-101 expression in colorectal cancer cell lines. The recombined lentiviral vector GV209-mir101 or the empty lentiviral vector GV209 was transfected into human colorectal cancer cells SW620. The recombinant psiCHECK-2-Rac1 vector containing RAC1 3'UTR was constructed, and site-directed mutagenesis of RAC1 3'UTR was induced to construct the psiCHECK-2-Rac1-Mut vector. In HEK293A and SW480 cells co-transfected with mir-101 inhibitors or negative control (NC) and these recombined vectors, luciferase activities was examined with a dual-luciferase reporter assay.</p><p><b>RESULTS</b>SW620 cells transfected with GV209-mir101 lentivirus exhibited higher mir-101 expression level than cells transfected with GV209 lentivirus. Mir-101 inhibitors significantly increased the luciferase activities of RAC1 3'UTR. Overexpression of mir-101 increased the expression of RAC1 while inhibition of mir-101 suppressed RAC1 expression.</p><p><b>CONCLUSION</b>We have successfully constructed a SW620 cell line stably overexpressing mir-101. mir-101 can suppress RAC1 gene expression by targeting the specific sequence of RAC1 3'UTR.</p>

Preliminary screening of downstream proteins of Sox2 and role of Sox2 in colonic cancer cell migration and invasion / 南方医科大学学报

<p><b>OBJECTIVE</b>To screen the down-stream proteins of transcription factor Sox2 and explore the role of Sox2 in the proliferation and migration of colonic cancer cells in vitro.</p><p><b>METHODS</b>The cellular proteins were separated by SDS-PAGE electrophoresis and stained with Coomassie blue and amine plated silver. The differentially expressed proteins was identified by mass spectrometry and verified by QPCR and Western blotting. A cell counting kit-8 (CCK8) assay was performed to evaluate the cell proliferation, and the cell migration was assessed using Transwell assay.</p><p><b>RESULTS</b>S3a was identified by proteomics technology as a Sox2-downregulated protein while ENO1 and gama-actin the up-regulated proteins. QPCR and Western blotting analyses showed that overexpression of Sox2 significantly decreased the expression of S3a (P<0.005) and increased the expression of ENO1(P<0.05), but had no significant effect on gama-actin expression. Sox2 overexpression obviously promoted cell proliferation and migration (P<0.05), while inhibition of Sox2 produced contrary effects (P<0.05).</p><p><b>CONCLUSION</b>Sox2 negatively regulates S3a expression and positively regulates ENO1 expression to promot the proliferation and migration of colonic cancer cells.</p>

Expression of hsa-miR-186 and its role in human colon carcinoma cells / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the expression of hsa-mir-186 in colorectal cancer and study its role in regulating the biological behaviors of human colorectal cancer SW620 cells in vitro.</p><p><b>METHODS</b>The expression of hsa-miR-186 in colon cancer tissue and the adjacent tissues as well as 5 colon carcinoma cells were analyzed using real-time quantitative RT-PCR. The precursor sequence of miR-186 gene was amplified from the genomic DNA by PCR and cloned into the lentiviral vector PLVTHM labeled with GFP. The colorectal cancer cell line SW620 was transfected with PLVTHM-miR186 vector and the lentivirus-infected cells were sorted with flow cytometry. Cell counting kit-8 (CCK-8) assay was used to detect the proliferation of the cells. The migration and invasion of SW620 cells were investigated using Transwell assay and scratch test. Western blotting was used to detect the expression of YY1 protein in SW620 cell lines.</p><p><b>RESULTS</b>The relative expression of miR-186 in the cancer tissues was 0.0024∓0.0027, significantly lower than that in the adjacent tissues (0.066∓0.068, P=0.008); the relative expression level of hsa-miR-186 in SW620 and LoVo cells with a high metastatic potential was 0.118∓0.138 and 0.157∓0.001, respectively, significantly lower than that in HT-29 cells with a low metastatic potential (1.000∓0.00, P<0.05). The recombinant lentiviral vector PLVTHM-miR186, verified by enzyme digestion, sequencing and qPCR, caused significant inhibition of cell proliferation, migration and invasion and suppressed the expression of YY1 protein in SW620 cells.</p><p><b>CONCLUSION</b>As a tumor suppressor gene, Hsa-miR-186 is down-regulated in colon carcinoma tissues and in highly metastatic SW620 and LoVo cells. Has-miR-186 can inhibit the cell proliferation, migration and invasion of colon carcinoma cells in vitro possibly by suppressing YY1 expression.</p>

Effect of remifentanil on cell apoptosis during renal ischemia/reperfusion in rats / 中华麻醉学杂志

Objective To evaluate the effect of remifentanil on cell apoptosis during renal ischemia-reperfusion (I/R) in rats.Methods Seventy-five male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 3 groups (n =25 each):sham operation group (group S),I/R group,and remifentanil group (group R).Renal ischemia was induced by occlusion of the bilateral renal arteries for 45 min followed by reperfusion in groups I/R and R.Remifentanil was infused at 1.0 μg· kg-1 · min-1 via the caudal vein starting from 15 min before ischemia until 30 min of reperfusion in group R,while the equal volume of normal saline was given instead of remifentanil in groups S and I/R.At 15 min before ischemia (T0) and 3,6,12,24 h of reperfusion (T1-4),5rats were anesthetized and sacrificed,and renal specimens were obtained to detect the apoptotic rate and expression of Bax and Bcl-2 protein (by flow cytometry) and mRNA (by RT-PCR).The ratios between Bcl-2/Bax protein and mRNA expression were calculated.The pathological changes of renal tubules were scored.Results Compared with group S,the pathological scores and apoptotic rate were significantly increased at T1-4,and ratios between Bcl-2/Bax protein and mRNA expression were increased at T1,2,while decreased at T3,4 in groups R and I/R (P ＜0.01).Compared with group I/R,the pathological scores and apoptotic rate were significantly decreased at T1-4,while the ratios between Bcl-2/Bax protein and mRNA expression were increased in group R (P ＜ 0.05 or 0.01).Compared with the baseline value at T0,the pathological scores and apoptotic rates were significantly increased at T1 4,and the ratios of Bcl-2/Bax protein and mRNA expression were increased at T1,2,while decreased at T3,4 in groups R and I/R (P ＜ 0.01).Conclusion Regulation of Bcl-2/Bax expression and inhibition of cell apoptosis in renal tissues are involved in the mechanism by which remifentanil reduces renal I/R injury in rats.

Effect of isoflurane on apoptosis of SH-SY5Y cells transfected with APPsw gene and the role of inositol 1,4,5-triphosphate receptors / 中华麻醉学杂志

Objective To evaluate the effect of isoflurane on the apoptosis of SH-SYSY cells transfected with APPsw gene and the role of inositol 1,4,5-triphosphate (IP3) recepters.Methods The SH-SYSY ceils transfected with APPsw gene were seeded in culture flasks with the density of 1.2 × 104/cm2.The cells were randomly divided into 4 groups (n =6 each):control group (group C),IP3 receptor antagonist group (group Ⅹ),isoflurane group (group Ⅰ) and isoflurane + IP3 receptor antagonist group (group Ⅰ + Ⅹ).After the cells were cultured for 24 h and attached to the wall,the cells were cultured routinely in group C,and Xestospongin C 100 nmol/L (IP3 receptor antagonist) was added to DMEM culture medium in groups X and Ⅰ + X,and 30 min later the cells were exposed to 1.2 ％ sevoflurane for 8 h in groups Ⅰ and Ⅰ + X.The cells were collected for examination of the ultrastructure and for determination of cell apoptosis,intracellular free calcium ion concentration [Ca2 +] i (by flow cytometry) and expression of IP3 receptor protein (by Western blot).The apoptosis rate was calculated.Results Compared with group C,there was no significant change in the apoptosis rate,[Ca2 +]i or IP3 receptor protein expression in group Ⅹ (P ＞ 0.05),while the cell apoptosis rate and [Ca2 +] i were significantly increased and IP3 receptor protein expression was up-regulated in groups I and Ⅰ + Ⅹ (P ＜ 0.05 or 0.01).Compared with group Ⅰ,cell apoptosis rate and [Ca2+]i were significantly decreased and IP3 receptor protein expression was down-regulated in group Ⅰ + Ⅹ (P ＜ 0.01).The pathological changes of the cells happened in groups Ⅰ and Ⅰ + Ⅹ,and the pathological changes were severer in group Ⅰ than in group Ⅰ + Ⅹ.Conclusion Isoflurane can induce apoptosis of SH-SY5Y cells transfected with APPsw gene through increasing [Ca2+]i and up-regulating IP3 receptor protein expression.

Effect of sodium ferulate on injury of gastric mucosa after traumatic hemorrhage shock resuscitation in rabbits / 中华急诊医学杂志

Objective To investigate the effect and mechanism of sodium ferulate (SF) on injury of gastric mucosa after traumatic hemorrhage shock resuscitation in rabbits.Methods One hundred healthy rabbits were randomly ( random number) divided into 4 groups ( n =25 in each group):control group ( A),model group ( B),pre-resuscitation SF group (C) and post-resuscitation SF group (D).The gastric mucosa injury model was established by using a method of comminuted fracture of femur and blood depletion.SF 30 mg/kg was injected into vein of rabbits' ear 20 min before resuscitation in group C and 30 min after resuscitation in group D,while rabbits of remaining groups received equal volume of normal saline instead.The gastric mucosa was obtained 90 min after resuscitation.The damage index (DI) of gastric mucosa was observed with method of Guth and the ultra-structure of parietal cell of stomach was observed under electronic microscope and the contents of TXB2 and 6-Keto-PGF1α in gastric tissue homogenate were determined with radio-immunity methods,and the ratios of TXB2/6-Keto-PGF1α were calculated.Data were analyzed by ANOVA ( LSD-t test ),and P ＜ 0.05 was considered as statistical significance. Results Under the electronic microscope,the secreting tubules were observed to be closed tightly in the parietal cells of stomach in the group A,showing a static status.However,in the group B,the number of normal secreting tubules was increased and the lumens were enlarged obviously.Compared with the group B,the number of normal secreting tubules was decreased and the enlargement of secreting tubules was not obvious in group C.The degree of changes in secreting tubules in group D was that between group C and group B.Compared with group A,the DI,the content of TXB2 and ratio of TXB2 to 6-Keto-PGF1α in other three groups were higher [DI: (81.5+13.6), (61.3+18.2), (70.5+17.2) vs.(4.2+2.7); the contents of TXB2:(4.95 +0.51),(3.75+0.64),(4.39±0.69) vs.(2.76±0.44); and the ratios of TXB2 to 6-KetoPGF1α:(0.064±0.002),(0.037±0.005), (0.049±0.002) vs.(0.027±0.002)] (P＜0.01),but the contents of 6-Keto-PGF1α in other 3 groups were lower [ (77.9±8.9),(96.4±11.2),(89.2+11.4) vs. (109.3±7.6)] (P＜0.05orP＜0.01).Compared with group B,theDI [ (61.3±18.2),(70.5±17.2) vs.(81.5±13.6)] and the contents of TXB2 [ (3.75±0.64), (4.39±0.69) vs.(4.95±0.51)] and the ratios ofTXB2 to6-Keto-PGF1α [ (0.037±0.005), (0.049±0.002) vs.(0.064 ±0.002)] in groups C and D were lower (P ＜ 0.05 or P ＜ 0.01 ),but the contents of 6-KetoPGF1α in groups C and D [ (96.4 ± 11.2),( 89.2 ± 11.4) vs.(77.9 ± 8.9) ] were higher ( P ＜ 0.05 or P ＜ 0.01 ).Compared with group C,the DI [ ( 70.5 ± 17.2) vs.61.3 ± 18.2) ] and the contents of TXB2 [ (4.39 ± 0.69) vs.(3.75 ± 0.64) ] and the ratios of TXB2 to 6-Keto-PGF1α [ (0.049 ± 0.002 ) vs.(0.037 +0.005) ] in group D were higher ( P ＜ 0.05 or P ＜ 0.01 ),but the content of 6-Keto-PGF1α in group D [ ( 89.2 ± 11.4 ) vs.(96.4 ± 11.2) ] was lower ( P ＜ 0.05 ).Conclusions SF can attenuate the injury of gastric mucosa after traumatic hemorrhage shock resuscitation in rabbits,and its therapeutic effects is better when it is administered before resuscitation than those as it is administered after resuscitation.The possible mechanism is associated with the effects of improving balance between TXB2 and 6-Keto-PGF1α and inhibiting the secreting function of parietal cell of stomach.

Effects of propofol on gastric mucosal cellular apoptosis after resuscitation of hemorrhagic shock in rabbits / 中华麻醉学杂志

Objective To investigate the effects of propofol on gastric mucosal cellular apoptosis after resuscitation of hemorrhagic shock in rabbits.Methods One hundred healthy adult male New Zealand rabbits weighing 2.5-3.0 kg were randomly divided into 5 groups ( n =20 each):group sham operation (group S) ; group hemorrhagic shock ( group M ) and Ⅲ,Ⅳ,Ⅴ 3 propofol groups ( groups P1,2,3 ).Hemorrhagic shock was induced by withdrawing blood from femoral artery.MAP was reduced to 35-40 mm Hg and maintained at this level for 60 min in groups M,P1,P2 and P3.Blood was then transfused back via femoral vein to restore blood volume.In groups P1,2,3 propofol 5 mg/kg was injectel iv at 10 min before ischemia (group P1 ),10 min before (group P2 ) and 20 min of resuscitation (group P3 ) respectively followed by continuous infusion at 20 mg·kg-1 ·h-1 until 90 min of resuscitation.The gastric mucous membrane specimens were obtained at 90 min of resuscitation for macroscopic examination and detection of apoptosis (by TUNEL) and Bcl-2 and Bax protein expression (by immuno-histochemistry).Results Hemorrhagic shock seriously damaged gastric mucous membrane,significantly increased apoptotic index (the number of apoptotic cells/the total number of cells) and Bax protein expression and decreased Bcl-2 protein expression and Bcl-2/Bax ratio in group M as compared with group S.Propofol significantly attenuated hemorragic shock-induced above changes in groups P1 and P2.Concluion Pre- and post-conditioning with propofol can attenuate apoptosis in gastric mucous membrane cells induced by hemorrhagic shock by up-regulating Bcl-2 protein expression and down-regulating Bax protein expression.

Role of opioid receptors in remifentanil-induced attenuation of renal ischemia/reperfusion injury in rats / 中华麻醉学杂志

Objective To investigate the role of opioid receptors in remifentanil-induced attenuation of renal ischemia/reperfusion (I/R) injury in rats.Methods Seventy-five male Sprague-Dawley rats weighing 250-300 g were randomly divided into 5 groups ( n =15 each):sham operation group (group S),group I/R,remifentanil group (group R),naloxone group (group N),and naloxone + remifentanil group (group NR).Renal ischemia was induced by clamping the bilateral renal arteries for 45 min using an atraumatic clamp followed by reperfusion.In groups R and NR,remifentanil was infused at 1.0 μg· kg-1 · min-1 via the caudal vein starting from 15 min before ischemia until 30 min of reperfusion,while groups S,I/R and N received the equal volume of normal saline instead of remifentanil.In groups N and NR,naloxone 0.3 mg/kg was injected via the caudal vein at 20 min before ischemia and at 35 min after ischemia respectively,while groups S,I/R and R received the equal volume of normal saline instead of naloxone.Blood and urine samples were collected from the femoral vein and urinary bladder respectively at 24 h of reperfusion for determination of the levels of serum creatinine (Cr) and blood urea nitrogen (BUN),urinary N-acetyl-β-D-glucosaminidase (NAG) and γ-glutamyl transpeptidase (γ-GT).The rats were sacrificed at 24 h of reperfusion and the renal tissues were removed for determination of nalondialdehyde (MDA) content and superoxide dismutase (SOD) activity.Pathological changes in renal tissues were observed with light microscope.Results Compared withgroup S,the levels of serum Cr and BUN,urinary NAG and γ-GT,and MDA were significantly increased,while the activity of SOD was significantly decreased in the other 4 groups ( P ＜ 0.05 or 0.01 ) and pathological changes in renal tissues were observed in the other 4 groups.Compared with group I/R,the levels of serum Cr and BUN,urinary NAG and γ-GT levels,and MDA were significantly decreased,while the activity of SOD was significantly increased ( P ＜ 0.01 ),and the pathological changes were reduced in group R,and no significant change was found in the parameters mentioned above in groups N and NR ( P ＞ 0.05).The pathological changes were similar in groups I/R,N and NR.Compured with group R,serum Cr and BUN concentrations,urinary NAG and γ-GT levels and MDA concent were increased,while SOD activity were decreased ( P ＜ 0.05 or 0.01 ).Conclusion Opioid receptors mediate remifentanil-induced attenuation of renal I/R injury in rats.

Effect of remifentanil on nucleotide-binding oligomerization domain 1 mRNA expression in rats with renal ischemia-reperfusion injury / 中华麻醉学杂志

Objective To investigate the effect of remifentanil on nucleotide-binding oligomerization domain 1 (NOD1) mRNA expression in rats with renal ischemia-reperfusion (I/R) injury.Methods Sixty male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 3 groups (n =20 each):sham operation group (S group),I/R group and remifentanil group (R group).Renal ischemia was induced by occlusion of bilateral renal arteries for 45 min followed by 24 h reperfusion in groups I/R and R.Remifentanil 1.0 μg· kg-1 · min-1 was infused until 30 min of reperfusion starting from 15 min before ischemia in group R,while the equal volume of normal saline was given instead in S and I/R groups.The animals were sacrificed at 15 min before ischemia and at 3,6,24 h of reperfusion and the kidneys were removed for microscopic examination and polymorphonuclear leukocyte (PMN) count and for measurement of NOD1 mRNA expression (by RT-PCR).The apoptotic rate was determined by flow cytometry double staining method.Results Compared with group S,NOD1 mRNA expression was up-regulated,and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion in group I/R,and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion,and NOD1 mRNA expression was up-regulated at 6 and 24 h of reperfusion in group R (P ＜ 0.01).Compared with I/R group,NOD1 mRNA expression was down-regulated,and the apoptotic rate and PMN count were significantly decreased at each time point during reperfusion (P ＜ 0.05 or 0.01),and the pathological changes were significantly attenuated in group R.Conclusion Remifentanil can reduce the renal I/R injury by down-regulating the expression of NOD1 mRNA and inhibiting inflammatory response and apoptosis.

Effects of different doses of remifentanil on renal ischemia-reperfusion injury in rats / 中华麻醉学杂志

Objective To investigate the effects of different doses of remifentanil on the renal ischemiareperfusion (I/R) injury in rats. Methods Sixty male SD rats weighing 220-250 g were randomly divided into 5 groups ( n = 12 each): sham operation group (group S), model group (group M), low, median and high doses of remifentanil groups (RL, RM and RH groups). The rats were anesthetized with intraperitoneal 5% chloral hydrate 6 ml/kg. Renal ischemia was induced by clamping the bilateral renal arteries for 45 min using an atraumatwere infused via the caudal vein 15 min before ischemia respectively and the infusion was stopped at 30 min of reperfusion, while S and M groups received equal volume of normal saline instead. Blood samples were collected from the femoral vein at 30 min and 24 h of reperfusion for measurement of serum creatinine (Cr) and blood urea nitrogen (BUN) concentrations. The rats were sacrificed at 24 h of reperfusion and the renal tissues were removed for determination of MDA content, SOD and Ca2+ -ATPase activities. Pathological changes in renal tissues were observed with light and electron microscopes. Results Compared with group S, the concentrations of serum Cr and BUN and content of MDA were significantly increased, while activities of SOD and Ca2+ -ATPase were significantly decreased in the other 4 groups ( P ＜ 0.05 or 0.01). Compared with group M, the concentrations of serum Cr and BUN and content of MDA were significantly decreased, activities of SOD and Ca2+ -ATPase were significantly increased (P ＜0.05 or 0.01) and the pathological changes were reduced in RH, RM and RL groups. The plasma BUN and Cr concentrations and MDA content were decreased gradually and SOD and Ca2+ -ATPase activities were increased gradually with the increase in the doses of remifentanil in RL, RM and RH groups ( P ＜ 0.05 or 0.01 ).Remifentanil infusion significantly attenuated the pathologic changes in a dose-dependent manner. Conclusion Remifentanil can reduce the renal I/R injury in a dose-dependent manner by inhibiting lipid peroxidation and increasing Ca2+ -ATPase activity.

Effects of different concentrations of isofluraue on viability in rat primary cortical neurons / 中华麻醉学杂志

Objective To investigate the effects of different concentrations of isoflurane on viability in rat primary cortical neurons. Methods Primary cortical neurons were isolated from neonatal Wistar rats (less than 24 h after birth) and exposed to 0.6 %, 1.2 % and 2.4 % isoflurane for 1, 2, 4, 8, 12 or 24 h, cell viability was evaluated by MTT reduction and LDH release assays. Changes in intracellular [Ca2+]i were detected by real-time confocal microscopy after the neurons were exposed to different concentrations of isoflurane. Results Exposure to 0.6% isoflurane for 24 h significantly increased viability of the primary rat cortical neurons as shown by decrease in LDH release and increase in MTT and elevation of peak [Ca2+]i. Exposure to 1.2% isoflurane for 12 or 24 h and to 2.4% isoflurane for 8, 12 or 24 h significantly reduced viability of cortical neurons associated with high and fast elevation of peak [Ca2 +] i. Conclusion Exposure to 0.6 % isoflurane increases viability of the primary rat cortical neurons. Exposure to 1.2% isoflurane and to 2.4% isoflurane decreases cell viability and the mechanism may be related to the changes in calcium concentrations in the neurons.

The Dynamical Progeression and Clinical Significance of S100B and IL-1?of Cerebrospinal Fluid in Patients with Tuberculous Meningitis / 医学研究杂志

Objective To explore the dynamical progeression and clinical significance of S100B and IL-1? of cerebrospinal fluid in patients with tuberculous meningitis.Methods 43 patients with TM(TM group)treated with tubercle drug and 28 patients without never diseases(control group)have been studied.S100B in Cerebrospinal Fluid were exeminated by ELISA and IL-1?by radio-immunassay.Results S100B or IL-1? of cerebrospinal fluid in patients with tuberculous meningitis increased evidently in contrast with the control group.And S100B or IL-1? of Cerebrospinal Fluid in coma patients increased evidently in contrast with no coma.Conclusion The levels of S100B in CSF could reflex the degree of damage in patients with TM.IL-1? was more sensitive to treatment.

Antibacterial and Anti-inflammatory Effects of GanKeQing granules:An Experimental Study / 中华医院感染学杂志

OBJECTIVE To discuss the antibacterial and anti-inflammatory effects of GanKeQing granules.METHODS The antibacterial tests in vitro,and the tests of it′s affects for auricular swelling induced by xylene and celiac capillary permeability induced by acetic acid in mice were done to observe the pharmaceutical effects of GanKeQing granules.RESULTS GanKeQing granules could inhibit and kill 5 kinds of pathogenic bacteria,suppress the swelling of the mouse′s ear caused by xylene and releave the enhancment of mice capillary permeability caused by acetic acid.CONCLUSIONS GanKeQing granules have better antibacterial and anti-inflammatory effects,which could be used for the treatment and prevention of cold and flu.

Antibiotics Utilization among Non-surgical Patients in Respiratory Department of Our Hospital / 中华医院感染学杂志

OBJECTIVE To find out the actual situations of antibiotics utilization in Respiratory Department of our hospital and provide feedback of improper applications to the clinic,so as to promote proper utilization of antibiotics.METHODS To take samples from clinical cases of 141 samples in Oct to Nov 2006 and fill the forms of the basic information and investigation items of General Hospital of Chinese People′s Armed Police Forces,the cases were finally sorted and summarized with Excel.RESULTS The antibiotics utilization ratio was 96.5%,their combination usage accounted for 70.2%,the etiology detection rate was 61.7%,the improper usage accounted for 34.8%.CONCLUSIONS The situation of antibiotics utilization in our hospital has changed a lot.Hospitals should define regulations and reinforce the management according to The Principle Guidelines of Antibiotics Utilization and actual situations.

Interaction Between Anthracene Quinone Type Anti-tumor Antibiotics and DNA / 中华医院感染学杂志

OBJECTIVE To study the interaction of anthracene quinone type anti-tumor antibiotics and DNA as well as the experiment method.METHODS Spectrophotometry was developed including absorption spectrum,fluorescence spectrum,electrochemical method and so on.RESULTS The union of inlay and insertion observed in ultraviolet and visible spectrophotometry usually caused hypochromic effect and red shift.Under the certain concentrations of bovin serum albumin(BSA),the endogene fluorescence intensity of BSA orderly reduced with the increase in concentration of doxorubicin(adriamycin) hydrochloride(?em 344 and the peak shape were invariable);?ex and ?em at the biggest wave length of doxorubicin were 478 and 596 nm.The fluorescence intensity was maximal of the excitation and emission spectrum when pH was 3.0.CONCLUSIONS The interaction of doxorubicin and DNA is the strongest according to the experiment and is the most widely used at present in clinics.

Effects of Iotensin premedication on sympathetic responses and plasma catecholamine concentration in cervical plexus block / 中华麻醉学杂志

Objective: To evaluate the effects of lotensin on sympathetic responses following cervical plexus block and to explore the mechanism of cardiovascular responses and sympathetic nervous system activity according to plasma catecholamine concentration. Method: Sixty adult patients were randomly divided into two groups (n = 30):Group A and group B. In group B lotensin 0.15mg/kg was taken orally at night before surgery and 2h before anesthesia separately. Hemodynamics was determined before anesthesia(T_1), 5min(T_2), 10min(T_3), 15-20min(T_4)and 30min (T_5) after cervical plexus block. For measurement of plasma catecholamine concentration, blood was collected at T_1,T_3,T_4 and T_5. The study was finished before beginning of surgery and infusion. Result: In group A after anesthesia SP,DP, MAP,HR,RPP and plasma noradrenalinc level rose markedly(P0.05). All parameters were lower in group B compared with group A(P

Effects of Monosodium Glutamate on Survival and Reproductive Ability of Drosophila melanogaster / 环境与健康杂志

Objective To study the effects of monosodium glutamate on viability and fertility of filial generation Drosophila.Methods Different concentrations(15,30,45 g/L)of L,H,T monosodium glutamate(MSG)were added in the medium for wildtype Drosophila melanogaster,and the non-MSG medium was taken as the control group,3 pairs of Drosophilas in each bottle and 3 repeats in each group,the first and second filial generation adult Drosophila's eclosion time(growth period) and the number of offspring within 7 days(reproduction quantity),and the death count in 30 days of 20 Drosophilas were observed and recorded.Results Compared with the control group,the reproduction quantity of Drosophila significantly increased,the growth period shortened in the first filial generation in 15 g/L of T MSG group.As the MSG concentrations increased,the reproduction quantity reduced significantly,the survival rate of Drosophila in 30 days declined significantly.The reproduction quantity in second filial generation increased at the initial stage of exposure and then decreased as the MSG concentrations increased.Conclusion The results of the present paper indicates that low-dose of MSG may be the advantage for survival and reproduction of Drosophila,but high doses will produce adverse effects.