الملخص
Objective To explore the genome-wide distribution of histone H3K27ac in intestinal type gastric cancer,analyze remodeling features of enhancers and regulome and construct a prediction model for prognosis.Methods H3K27ac CUT&Tag sequencing and RNA sequencing were performed in intestinal type gastric cancer tissues from 15 patients and normal gastric mucosa tissues from 18 healthy volunteers.Bioinformatics analysis was performed to identify the differences in genome distribution of H3K27ac modifications.Based on the distribution characteristics of H3K27ac,the enhancer elements were identified and the remodeling characteristics of enhancer and related regulome were explored.The prediction model for prognosis based on enhancer related target genes was constructed by univariate Cox and multivariate Cox regression analyses.Results The histone H3K27ac modification was mainly distributed in the enhancer region and displayed no significant differences in the genomic distribution patterns between normal and cancer tissues.Compared with normal gastric mucosa,the level of enhancer H3K27ac modification was higher in intestinal type gastric cancer.A total of 8847 enhancers with increased activity in intestinal type gastric cancer were identified,accounting for 8.3%of all enhancers,which might promote malignant behaviors such as proliferation and adhesion of gastric cancer cells.A prognosis-predicting model established based on a panel of 6 genes that upregulated by the acquired enhancer in cancers,which was able to predict the overall survival of patients.Conclusion Enhancer remodeling is one of the significant epigenetic features of intestinal type gastric cancer.These enhancers may drive malignant growth and adhesion of cancer cells by upregulating the expression of MYC,E2F3 and other genes.A prognosis model based on enhancer target genes is constructed.
الملخص
Objective To explore the correlated risk factors of infants with severe bronchiolitis.Methods Two hundred and nine bronchiolitis children were enrolled in this study.According to the severity of the disease,children were divided into common group (n =182) and severe group (n =27),and then monofactorial analysis and non-conditional logistic analysis were performed to evaluate the risk factors in children with severe bronchiolitis.Results Monofactorial analysis showed that six variables were found on the occurrence of severe bronchiolitis with statistical significance including age (t =3.455 ; P =0.001),passive smoking (x2 =6.119 ; P =0.013),prematurity (x2 =17.124 ; P =0.000),obesity (x2 =5.673 ; P =0.017),allergic constitution (x2 =5.736 ; P =0.017),and congenital heart disease (x2 =20.694; P =0.000).Non-conditional logistic analysis showed that frequent occurrence of young age (OR 0.742 ; 95 ％ CI 0.601 ～ 0.917),passive smoking (OR 3.300 ;95 ％ CI 1.060 ～ 10.276),prematurity (OR 5.265 ;95 ％ CI 1.565 ～ 17.705) and congenital heart disease(OR 13.634 ;95％ CI 3.015 ～ 61.663)were the risk factors in children with severe bronchiolitis.Conclusion The factors that contribute to increase risk of severe bronchiolitis are young age,passive smoking,prematurity,allergic constitution and congenital heart disease.