The correlation between FCER2 gene polymorphism and the efficacy of inhaled corticosteroids in patients with chronic rhinosinusitis / 临床耳鼻咽喉头颈外科杂志

Objective:To investigate the correlation between FCER2（2206A>G） gene polymorphism and the efficacy of inhaled corticosteroids（ICS） in patients with chronic rhinosinusitis（CRS）. Methods:A total of 208 CRS patients were routinely treated with functional endonasal sinus surgery and postoperative ICS. DNA extraction, PCR amplification and gene sequencing were performed to observe the FCER2（2206A>G） gene polymorphism and calculate the allele frequency. The visual analog scale（VAS） score, Lund-Kennedy score, and computed tomography（CT） Lund-Mackay score were determined 6 months after surgery among patients with different genotypes. Moreover, the polymorphism frequency was compared among different subgroups（chronic rhinosinusitis with nasal polyps versus chronic rhinosinusitis without nasal polyps, eosinophilic chronic rhinosinusitis versus non-eosinophilic chronic rhinosinusitis）. Results:There were FCER2（2206A>G） gene polymorphism in patients with CRS, and the phenotypes included 3 genotypes, AA, AG and GG, with distribution frequencies of 68（32.7%）, 116（55.8%） and 24（11.5%） cases, respectively. No significant differences were found in age, VAS score, nasal endoscopic Lund-Kennedy score and CT imaging Lund-Mackay score among patients with CRS of each genotype before surgery. In patients with the AA genotype, the changes in VAS score（5.74±1.10）, Lund Kennedy score（5.92 ± 1.14）, and CT imaging Lund-Mackay score（13.26±4.26） were significantly higher than in patients with the AG（4.37±0.86, 5.37±1.24, 10.82±3.77） and GG（4.26±0.80, 5.18±1.56, 10.10±3.53） genotype（P<0.05）. However, there were no marked difference between patients with the AG genotype and those with the GG genotype（P>0.05）. Compared with patients with non-eosinophilic sinusitis, Among them, the differences between the GG genotype and AG /AA genes were more significant in eosinophilic sinusitis compared to non-eosinophilic sinusitis（P<0.01）. Conclusion:The FCER2（2206A>G） gene in patients with CRS has genetic polymorphism and is associated with the recovery of CRS patients after surgery, individual corticosteroid sensitivity, and subgroup variability.

Correlation between FCER2 gene rs28364072 polymorphism and asthma / 实用医学杂志

Objective To determine whether rs28364072 polymorphism in the low-affinity IgE receptor (FCER2)is associated with asthma risk in Chinese Han nationality asthmatics.Methods 120 cases of asthmatics and 116 healthy control subjects were enrolled in this study.DNA was purified from peripheral blood,and genotyping of rs28364072 SNP was performed by PCR and Sanger sequencing.The clinical indexes were compared between asthmatic subjects.Results A significant difference was found in the distributions of the genotyges (TT,TC,CC)and allele frequency among populations in north Indian,Saudi Arabian,Romas and Chinese Han nationality.A significant difference was also found in the distributions of the genotyges (TT,TC,CC) between asthmatic subjects and controls.Significant difference was observed in the allele (T/C) frequency between asthmatic subjects and healthy controls.The presence of C allele of GLCCI1 gene was found to be a greater risk factor in asthmaticsubjects compared with the healthy controls.The odds ratio (OR) of CC and CC+TC were 2.73 (1.19 ～ 6.23),2.05 (1.21 ～ 3.48),respectively.There was significant difference in the ACT score,EOS％ between CC and TT genotyge.Conclusion The minor alleles C of rs28364072 SNP was significantly associated with the increase of asthma risk in asthma patients of of Han Nationality of Chinese.