الملخص
Objective:To optimize the extraction process of Shenghua pills and establish the best method for the TLC identification and the HPLC determination of effective components to provide an effective method and reliable basis for the quantitative and qualitative analysis of Shenghua pills. Methods:TLC was used for the identification of angelica,Rhizoma Chuanxiong and Licorice. Ferulic acid was determined by HPLC,and the separation was performed on an Agilent 5 TC- C18(2)(250 mm×4.6 mm,5 μm)column using a mixture of methanol-5% acetic acid solution(25 :75) as the mobile phase with the detection wavelength at 322 nm. The flow rate was 1.0 ml·min-1and the column temperature was room temperature. Results:Angelica sinensis(Oliv.) Diels.,Ligusticum chuanxiong Hort. and Glycyrrhiza uralensis Fisch. could be detected by TLC. Ferulic acid was linear within the range of 1.28-20.51 μg·ml-1(r=0.999 9). The average recovery was 98.47% with the RSD of 0.36% (n=6).Conclusion:The method can be used to efficiently extract the active components in Shenghua pills. The method is feasible,accurate and reproducible. It can be used for the quality con-trol of Shenghua pills.