Effect of low dose arsenious acid combined with total particulate matter of cigarette smoke on oxidative stress in human lung cancer cell line A549 / 公共卫生与预防医学

Objective To investigate the effect of low-dose arsenious acid solution (As(III)) combined with total particulate matter (TPM) from cigarette smoke on cellular oxidative stress in human lung cancer cell line A549 cells. Methods A549 cells were divided into four groups: negative control group (0.75% DMSO), low dose As(III) group (0.88% μg/mL, 75% DMSO), cigarette smoke TPM group (75 μg/mL), and combined exposure group (75 μg/mL TPM, 0.88 μg/mL As (III)). After 24 hours' exposure, the superoxide dismutase (SOD) level in cell culture medium and intracellular 8-hydroxy-2-deoxyguanosine (8-OHdG) content were detected by ELISA, and intracellular reactive oxygen species (ROS) level was detected by fluorescent probe DCFH-DA. 2×2 factorial design was used to evaluate the interaction. Results Compared with the control group, the level of SOD in the combined exposure group was significantly increased (P<0.05). In addition, the ROS content in the combined exposure group and TPM alone group was significantly increased (P<0.05). The levels of 8-OHdG in the combined exposure group and low-dose As(III) treated group were significantly higher than those in the control group(P<0.05). The results of the factorial analysis showed that low-dose As(III) and TPM had interaction on SOD levels, ROS and 8-OHdG contents in A549 cells. The effects on SOD and ROS were synergistic, while the effect on 8-OHdG was antagonistic. Conclusion Low-dose arsenious acid solution As(III) and TPM in cigarette smoke have interaction on oxidative stress in A549 cells.

Characteristics of Renal Cell Carcinoma Harboring TPM3-ALK Fusion

The World Health Organization 2016 edition assigned anaplastic lymphoma kinase (ALK) rearrangement-associated renal cell carcinoma (ALK-RCC) as an emerging renal tumor entity. Identifying ALK-RCC is important because ALK inhibitors have been shown to be effective in treatment. Here, we report the case of a 14-year-old young man with ALK-RCC. Computed tomography revealed a well-demarcated 5.3-cm enhancing mass at the upper pole of the left kidney. There was no further history or symptoms of the sickle-cell trait. The patient underwent left radical nephrectomy. Pathologically, the mass was diagnosed as an unclassified RCC. Targeted next-generation sequencing identified a TPM3-ALK fusion gene. The present report and literature review demonstrate that TPM3-ALK RCC may be associated with distinct clinicopathological features. Microscopically, the tumors showed diffuse growth and tubulocystic changes with inflammatory cell infiltration. Tumor cells were dis-cohesive and epithelioid with abundant eosinophilic cytoplasm and cytoplasmic vacuoles. If morphological features and TFE3 expression are present in adolescent and young patients, molecular tests for ALK translocation should be performed. This awareness is critically important, because ALK rearrangement confers sensitivity to ALK inhibitors.

A Family of Congenital Fiber Type Disproportion with Mutation in Tropomyosin 3 (TPM3) Gene Presenting as Altered Mentality with Respiratory Distress

Congenital fiber type disproportion (CFTD) has been related with mutations in ACTA1, SEPN1, RYR1 and tropomyosin 3 (TPM3) genes. Particularly, TPM3 mutation was identified as one of the most frequent cause of CFTD and was also detected in cap myopathy and nemaline myopathy. Herein we report patients of autosomal dominant TPM3 missense mutations with CFTD in a Korean family over twogenerations. Two of our patients, who developed mild muscle weakness in infancy, presented with altered mentality and respiratory distress despite relatively mild limb weakness.

Cap Myopathy With a Heterozygous TPM3 Missense Mutation

Cap myopathy is pathologically characterized by cap structures comprising well-demarcated areas under the sarcolemma and containing deranged myofibrils and scattered Z-disks. Clinically it presents with slowly progressive muscle weakness, myopathic face, and frequent respiratory insufficiency. Four genes have been reported to be associated with the disease: TPM2, TPM3, ACTA1, and NEB. Here we describe that a patient presenting with mild limb weakness with facial affection showed cap structures on muscle pathology and carried a heterozygous TPM3 mutation.

Caracterización del paciente que solicita atención de especialidad en periodoncia en una población chilena / Characteristics of patients requesting treatment at a periodontics specialty facility in a Chilean population

Objetivo: Realizar la caracterización de los pacientes que solicitan atención de especialidad en periodoncia. Material y métodos: Estudio descriptivo que incluyó a todos los pacientes inscritos para recibir atención odontológica de una población de la Quinta Región de Chile. Los datos se agruparon de acuerdo con el sexo femenino, masculino y rango de edad. Se registró ausencia o presencia de hábito de tabaco, diabetes mellitus (DM) tipo 2, ingreso a tratamiento periodontal, abandono de tratamiento e ingreso a terapia periodontal de mantenimiento (TPM). Para el análisis estadístico las diferencias en la frecuencia se determinó con χ2, alpha de 0.05, nivel de confianza de 95%, potencia de 80% y t Student evaluando diferencias en la edad, sexo femenino y sexo masculino. Resultados: Solicitaron atención 110 pacientes, 75 sexo femenino y 35 sexo masculino, ingresaron a la especialidad el 94%. La edad promedio fue de 41 (DE 15) años. Durante el seguimiento por un año, el 68% se mantuvo en tratamiento y el 19% ingresó a TPM. La tasa de DM tipo 2 fue de 4% en mujeres y 31% en hombres (p < 0.001). El rango de edad entre 14 a 29 años abandonó más el tratamiento periodontal. En relación con la DM tipo 2 las diferencias fueron estadísticamente significativas en la edad promedio, 39.5 (DE 15.3) años (p= 0.002) y edad promedio de abandono, 33.2 (DE 17.7) años (p > 0.001). Conclusiones: Las mujeres asisten más a tratamiento periodontal que los hombres y las personas jóvenes abandonan más el tratamiento. Al estudiar ambos sexos, masculino y femenino, los hombres alcanzaron un mayor porcentaje de DM tipo 2. Además el paciente diabético demostró ser más joven en edad promedio y en edad promedio de abandono de tratamiento periodontal.

Objective: To undertake characterization of patients requesting periodontic specialty care. Material and methods: The present was a descriptive study which included all patients registered to receive dental care in a population of the Quinta Region in Chile. Data were grouped according to male and female gender, as well as age range. The following was recorded: presence or absence of tobacco use habit, type II diabetes mellitus (DM), admission to periodontal treatment, treatment desertion and admission to posttreatment periodontal therapy (PTPT). For statistical analysis of frequency differences χ2 test, alpha 0.05, confidence level 95% potency 80% and t student test were used, assessing differences according to age and gender. Results: 110 patients requested treatment: 75 female and 35 male. 94% were admitted in the specialty program. Average age was 41 years (SD 15). During the one-year follow-up, 68% of patients remained in treatment, and 19% was admitted in PTPT. Type II DM rate was 4% for females and 31% for males (p < 0.001). Patients in age range 14-29 years were more prone to abandon periodontal treatment. With respect to type II DM differences were statistically significant in average age, 39.5 (SD 15.3) years (p = 0.002) and average age for desertion 33.2 (SD 17.7) years (p > 0.001). Conclusions: Women were more inclined to attend periodontal treatment than men, young patients deserted treatment more frequently. Studies on both genders revealed that males exhibited greater rate of type II DM. Additionally, diabetic patients were younger in average age as well as in average age for desertion of periodontal treatment.

Protective effect of topiramate on hypoxic-ischemic brain injury in neonatal rat

OBJECTIVE@#To explore protective effect of topiramate (TPM) on hypoxic-ischemic brain injury.@*METHODS@#A total of 360 neonatal rats were selected then randomly divided into sham operation group, ischemia and hypoxia group, conventional treatment group and degradation therapy group (n=90). After surgical treatment, sham and ischemic hypoxia group were treat with normal saline; conventional treatment group was received TPM solution 100 mg/kg, 2 times/d; degradation therapy group received TPM solution 150 mg/kg, 2 times/d, per 3 d treatment each dosage was reduced 50 mg/kg, the lowest reduced to 50 mg/kg. Four groups received continuous treatment for 10 d. After treatment for 1 d, 4 d, 7 d, 10 d the cerebral edema, neuron-specific enolase (NSE) and γ-aminobutyric acid (GABA) levels and cognitive abilities of four groups were observed.@*RESULTS@#After 1 d, 4 d of treatment, the brain water content and NSE levels in ischemia and hypoxia group, the conventional treatment group and the degradation therapy group were significantly higher than that in sham group (P<0.05), the brain water content and NSE levels of the conventional treatment group and the degradation therapy group were significantly lower than that in the ischemic hypoxia group (P<0.05). GABA levels and learning ability of the ischemia and hypoxia group, the conventional treatment group and degradation therapy group were significantly lower than the sham group (P<0.05), the GABA levels and learning ability of the conventional treatment group and degradation therapy group were significantly higher than the ischemia and hypoxia group (P<0.05). After 7 d, 10 d of treatment, the brain water content and NSE levels in the sham operation group, the conventional treatment group and degradation therapy group were significantly lower than the ischemia and hypoxia group (P<0.05), while the GABA levels and learning ability of these three groups were significantly higher than that in the ischemia and hypoxia group (P<0.05), the GABA levels in the conventional treatment group were significantly higher than degradation therapy group (P<0.05); After 10 d of treatment, the GABA levels of the conventional treatment group were significantly higher than the sham group, the learning ability of the degradation therapy group and sham operation group were significantly higher than the conventional treatment group (P<0.05).@*CONCLUSIONS@#The correct amount of short-term TPM has protective effect on hypoxic-ischemic brain injury, but long-term or excessive use may cause new damage to the brain and reduce the cognitive ability.

First Korean Family with a Mutation in TPM2 Associated with Sheldon-Hall Syndrome

Sheldon-Hall syndrome (SHS) is a rare autosomal dominant, inherited arthrogryposis syndrome characterized by multiple congenital contractures of the distal limbs. To date, four genes that encode the skeletal muscle fiber complex have been confirmed as the causative genes. Mutations in MYH3 have been identified most frequently and few cases of SHS caused by TPM2 mutations have been reported worldwide. This report describes, for the first time, a Korean family with two generations of SHS resulting from a rare TPM2 mutation, p.R133W. The affected mother and daughter manifested typical facial features of SHS including a triangular face with downslanting palpebral fissures, small mouth, high arched palate, and prominent nasolabial folds, and showed camptodactyly of fingers and deformities of feet with congenital vertical tali. Generalized myopathy with relative sparing of the slow-twitch muscle fibers was also revealed by electromyography in the affected mother.

Mídia e Sexualidade: a relação lésbica na revista TPM / Medios y sexualidad: la relación lésbica en la revista TPM / Media and sexuality: gay relationship in the magazine TPM

Relações homoafetivas ganham cada vez maior visibilidade na mídia brasileira, que se constitui como um rico acervo de costumes e valores, documentando e construindo a realidade social. Apesar disso, as revistas femininas não tratam do tema com frequência. O presente artigo analisa, por meio de técnicas de análise de discurso, de que maneira são apresentadas as relações lésbicas na revista TPM, única publicação brasileira que conta com uma coluna permanente escrita por uma jornalista assumidamente homossexual. Demonstra-se, por último, que a chamada "Coluna do Meio", assinada por Milly Lacombe, apresenta um modelo tradicional de relacionamento amoroso, que pressupõe estabilidade e monogamia.

Las relaciones homoafectivas adquieren cada vez mayor visibilidad en los medios de comunicación brasileños, que van constituyéndose en un rico acervo de costumbres y valores, documentando y construyendo la realidad social. A pesar de ello, las revistas femeninas no abordan el tema con frecuencia. Este artículo analiza, a través de técnicas de análisis del discurso, de qué manera son presentadas las relaciones lésbicas en la revista TPM, única publicación brasileña que cuenta con una columna permanente escrita por una periodista asumidamente homosexual. Por último, se demuestra que la llamada "Columna del Medio", de Milly Lacombe, presenta un modelo tradicional de relación amorosa, que presupone estabilidad y monogamia.

Homosexual relations are gaining greater visibility in the media, which is constituted as a rich collection of customs and values , documenting and constructing social reality. Nevertheless, women's magazines do not address the issue frequently. Thus, this article aims to analyze how lesbian relationships are presented in the magazine TPM, only Brazilian publication that has an ongoing column written by an openly gay journalist. Using techniques of discourse analysis, we investigated whether the column, named "Coluna do Meio", signed by Milly Lacombe, presents a traditional model of relationship, which implies stability and monogamy, which may make the subject more widely accepted by readers of that magazine.

The Results & Affecting Factors of Posterior Lumbar Interbody Fusion with TPM Cages in Spondylolisthesis / 대한척추외과학회지

STUDY DESIGN: This is a retrospective study analyzing the results of posterior lumbar interbody fusion(PLIF) with TPM cages in spondylolisthesis OBJECTIVES: The purpose of this study was to evaluate the clinical, radiologic results & affecting factors of PLIF with TPM cages in spondylolisthesis. SUMMARY OF LITERATURE REVIEW: The ordinary PLIF has 86-98% of radiologic union rate and clinical satisfactory rate. The problems of ordinary PLIF were graft breakage, donor site morbidity, limited bone resources, high failure rate using only transpedicular screw fixation. MATERIALS AND METHODS: From October 1995 to January 1999, 108 consecutive patients with spondylolisthesis were treated by PLIF with TPM cages, in which morcellized bone chips salvaged from posterior neural arch applied, and pedicle screw fixation. RESULTS: Preoperative Low Back Pain score(total 100 points) was improved from 47.4 points to 88.7 points at last follow-up. 10 point visual analogue scale was reduced to 1.5 point at last follow-up. In the end results, Ninty-two percent of patients rated as excellent or good. The preoperative value of slippage, 18.6% was achieved to 5.5% at last follow-up. The anterior intervertebral disc space height was increased from 10.0 to 16.2mm postoperatively. Fusion occurred in all patients except one. Groups below 60-year-old patient at the time of surgery showed better clinical results than those above 60 (p<0.05). No clear correlations were noted between sex, the types of spondylolisthesis, bone mineral density, smoking habits and obesity in clinical, radiologic results. CONCLUSION: PLIF with TPM cages is appeared to be a recommended procedure of choice to treat lumbar spondylolisthesis and this operation should be performed with a caution in aged patients.