Identification of TEAD1 Transcripts and Functional Analysis in Chicken Preadipocytes / 生物化学与生物物理进展

ObjectiveAlthough expression of the TEAD1 protein in preadipocytes has been established, its function remains unclear. In this study, we sought to detect transcripts of TEAD1 in chicken and to examine the effects of this protein on the proliferation, migration, apoptosis, and differentiation of immortalized chicken preadipocyte cell lines (ICP1). MethodsThe full-length sequence of the TEAD1 gene was cloned and the two transcripts were subjected to bioinformatics analysis. The subcellsular localization of TEAD1 transcripts was determined based on indirect immunofluorescence. The effects of TEAD1 transcripts overexpression on the proliferation of ICP1 cells were examined by RT-qPCR, CCK-8, and EdU assays; the effects of TEAD1 transcripts on ICP1 cells migration were examined based on the scratch test; and the effects of TEAD1 transcripts overexpression on ICP1 cells apoptosis were analyzed using apoptosis-Hoechst staining and RT-qPCR. The expression of TEAD1 transcripts in different tissues, cells lines, and ICP1 at different periods of differentiation was analyzed by RT-qPCR. The effects of TEAD1 transcripts overexpression on lipid droplet accumulation and adipogenic-related gene expression in ICP1 cells were analyzed based on Oil Red O and BODIPY staining, RT-qPCR, Western blot, and dual-luciferase reporter gene assays. Finally, the content of triglyceride (TG) was measured in TEAD1 overexpressed ICP1 cells. ResultsThe full-length TEAD1 was cloned and two TEAD1 transcripts were identified. The TEAD1-V1 protein was found to be localized primarily in the cell nucleus, whereas the TEAD1-V2 protein is localized in the cell cytoplasm and nucleus. The overexpression of both TEAD1-V1 and TEAD1-V2 significantly inhibited the proliferation of ICP1 cells. Whereas the overexpression of TEAD1-V1 promoted ICP1 cell migration, the overexpression of TEAD1-V2 had no significant effects on ICP1 migration; the overexpression of both TEAD1-V1 and TEAD1-V2 significantly promoted the apoptosis of ICP1 cells. We found that the different transcripts of TEAD1 have similar expression pattern in different tissues and cells lines. During induced preadipocyte differentiation, the expression of these genes initially declined, although subsequently increased. Overexpression of TEAD1-V1 promoted a significant reduction in lipid droplet formation and inhibited C/EBPα expression during the differentiation of ICP1 cells (P<0.05). However, the overexpression of TEAD1-V2 had no significant effect on lipid droplet accumulation or the expression of adipogenic-related proteins (P>0.05). Overexpression of TEAD1-V1 significantly decreased triglyceride content in ICP1 cells (P<0.05), while overexpression of TEAD1-V2 had no effect on triglyceride content in ICP1 cells (P>0.05). ConclusionIn this study, for the first time, identified two TEAD1 transcripts. Overexpressed transcripts TEAD1-V1 and TEAD1-V2 both inhibited the proliferation of chicken preadipocytes and promoted apoptosis of chicken preadipocytes. TEAD1-V1 inhibited the differentiation of preadipocytes and promoted the migration of preadipocytes, while TEAD1-V2 had no effect on the differentiation and migration of preadipocytes.

Comparative Performances of Improved Poultry Varieties under Backyard Rearing System in Dhemaji District of Assam

Productive and reproductive performances in terms of body weight, age at first egg, egg production, egg weight, fertility, hatchability and mortality of Vanaraja, Rainbow Rooster, Kamruapa and Desi chicken of Assam were studied under traditional backyard system of rearing. The Vanaraja, Rainbow Rooster and Kamruapa birds were given to rear under the frontline demonstration programmes of the Krishi Vigyan Kendra, Dhemaji to the women farmers of the district during 2020-21. The data on bird weights at different ages, egg weights, fertility, hatchability as well as mortality were collected and recorded and compared among the varieties. The data recorded from the study were analyzed as per standard statistical methods. The overall mean body weights were significantly (P < 0.05) higher in all the improved varieties than Desi chicken at different ages. There is also significant (P < 0.05) difference of age at first egg among Vanaraja (182.05±5.52 days), Rainbow Rooster (175.08±6.13 days), Kamruapa (163.06±5.52 days) and Desi chicken (203.31±3.31 days). The mean egg production values up to 32, 40, 52 and 72 weeks of age in the demonstrated varieties were significantly (P < 0.05) higher than the corresponding values of Desi birds. The mean egg weights of Vanaraja, Rainbow Rooster, Kamruapa at 32, 40 and 52 weeks of age were also significantly (P < 0.05) higher than Desi birds. However, no significant difference was observed in mortality rates among the four groups at different ages. There was also no significant (P < 0.05) difference in fertility and hatchability percent among the four genetic groups. It is concluded that Vanaraja, Rainbow Rooster and Kamruapa birds are adapted well under traditional backyard rearing system in agro-climatic conditions of Dhemaji district of Assam with adequate economic advantages over the Indigenous birds.

Detección y caracterización del virus de la anemia infecciosa aviar en Colombia

RESUMEN Objetivo. identificar el virus de la anemia infecciosa aviar (chicken anemia virus, CAV) en granjas avícolas y aves de traspatio en Antioquia, Colombia. Materiales y métodos. Se tomaron muestras de sangre y plumas de gallinas ponedoras; en cada granja se eligieron tres aves de seis edades diferentes (1, 15, 30, 60, 90 y 120 días de edad). También se obtuvieron muestras de aves de traspatio ubicadas cerca de las granjas estudiadas. Se realizó ELISA y PCR para el análisis de las muestras. Resultados. Mediante PCR, el 84% de las aves resultaron positivas al CAV en sangre total y el 66% en muestras de plumas. El 60% de las aves de traspatio dieron positivo en sangre y el 40% en folículo de pluma. Mediante ELISA, el 22% de las aves de las granjas avícolas presentó títulos de anticuerpos altos y el 19% moderados. En las aves de traspatio, el 43% presentó títulos de anticuerpos altos y 29% moderados. Además, los resultados de la prueba de RFLP y la secuenciación mostraron que el virus circulante encontrado en este estudio era diferente del de la cepa vacunal Cux-1 utilizada en el país. Conclusiones. El CAV está presente en Colombia en aves comerciales como de traspatio. Según los hallazgos, un alto porcentaje de las aves dieron positivo para la detección viral, aunque el número de aves positivas por anticuerpos fue bajo. Se requiere determinar las características del virus circulante para explicar la respuesta de anticuerpos obtenida.

ABSTRACT Objective. identify the presence of chicken anemia virus (CAV) in poultry farms and backyard chickens from Antioquia, Colombia. Materials and Methods. Blood and feather samples were taken from laying chickens; in each farm, three birds of six different ages (1, 15, 30, 60, 90, and 120 days old) were chosen randomly. Backyard chicken samples were also obtained near the research farms. We used serology and molecular techniques to analyze the samples. Results. By PCR, the 84% of the birds were positive in whole blood and 66% were positive in feather samples. The 60% of backyard chickens tested were positive in blood and 40% in feather follicle. By serology, the 22% of the poultry farm birds presented high antibody titers and 19% moderate antibody titers. In the backyard chickens, 43% of them presented high antibody titers and 29% moderate antibody titers. In addition, results from the RFLP test and sequencing showed that the circulating virus found in this study was different from the Cux-1 vaccine strain used in Colombia. Conclusions. CAV is present in Colombia in both commercial and backyard chickens. According to the findings, a high percentage of the birds tested positive for viral detection, whereas the number of birds that tested positive for antibodies was low. Thus, the characteristics of the circulating virus need to be determined to explain the antibody response observed in this study.

Impact of Cooking Conditions on Proximate Composition and Textural Properties of Chicken Breast Meat

The aim of this study was to evaluate the impact of cooking conditions on proximate composition and textural properties (cohesiveness and chewiness) of chicken breast meat. Eight packs of industrial skinless chicken breast meat were cooked by air frying (AF), baking (BK), deep fat frying (DF) and grilling (GR) at 170, 180 and 190oC for 0, 4, 8, 12 and 16 min. The chicken breast packs were frozen and sliced into dimensions, thawed, cooked and analysed by a two way analysis of variance. The results revealed that cooking methods significantly (p < 0.05) decreased moisture and protein contents from 75.14 to 58.25 % and 89.17 to 82.98 %, but increased fat content from 4.26 to 7.78 %, ash content from 1.95 to 2.39 %, carbohydrate content from 4.63 to 6.95 %, cohesiveness content from 0.40 to 0.52 and chewiness value from 3.63 to 6.05 kg. An increases in cooking temperatures and times decreased moisture content from 60.58 to 56.34 % and 75.14 to 47.40 % and protein content from 83.77 to 82.11 % and 89.17 to 79.45 %. Similarly, increases in cooking temperatures and times significantly (p < 0.05) increased fat content from 7.00 to 8.44 % and 4.26 to 10.12 %, ash content from 2.15 % to 2.59 % and 1.95 to 2.67 %. This study showed that increases in cooking temperatures decreased non-significantly (p > 0.05) carbohydrate content from 7.02 to 6.92 %, but increases in cooking times increased carbohydrate content from 4.63 to 7.76 %. An increases in cooking temperatures and times increased cohesiveness content from 0.50 to 0.54 and 0.40 to 0.63, chewiness value from 5.50 to 6.77 kg and 3.63 to 8.54 kg, respectively. There were no significant differences (p > 0.05) in chewiness values of samples cooked by AF and GR methods. The best cooking method/ temperature / time for low nutrient losses was BK, 170oC and 4 min

Response of 'criolla' potato (Solanum tuberosum) cultivar Colombia to mineral organic fertilization

The 'Criolla' potato (Solanum tuberosum) phureja group cultivated in the inter-Andean valleys of the high tropics, is commonly fertilized only with mineral nutrients in conventional production, without the use of soil test and use of organic fertilizers. The purpose of this study was to determine the potential of organic chicken manure as a source of nutrients to increase yield and quality in 'Criolla' potatoes compared to conventional fertilization. Treatments evaluated were organic chicken manure, mineral nutrition based on soil tests, organic manure mixed with mineral nutrition, and conventional nutrition based on farmers' typical nutrition plans. In plant response conventional and mineral treatments mixed with organic matter were the best in leaf area and dry weight of shoot and tuber. Treatment with mineral nutrition mixed with organic manure (2 and 6 t) obtained the largest length of the main stems. The best yield response was obtained with conventional nutrition, while the major size was obtained with mineral and mineral combined with organic treatments. According to the results, the best response was obtained with the application of mineral nutrition in the combination of 6 t ha-1 organic manure.

La papa criolla (Solanum tuberosum) grupo phureja es cultivada en los valles interandinos del trópico alto, comúnmente fertilizada con nutrientes minerales en la producción convencional, sin hacer uso de análisis de suelos y nutrición con fertilizantes orgánicos. El objetivo de este estudio fue determinar el potencial de la materia orgánica de gallinaza, como fuente de nutrientes, para incrementar el rendimiento y la calidad en la papa criolla, comparándola con la nutrición convencional. Los tratamientos evaluados fueron gallinaza, nutrición mineral, basado en análisis de suelos; nutrición mineral, combinada con materia orgánica y un tratamiento soportado en la fertilización convencional, con base en los planes nutricionales, típicos de los agricultores. En las respuestas de la planta, los tratamientos convencionales y minerales mezclados con nutrición mineral obtuvieron los valores más altos en área foliar y peso seco de la parte aérea y el tubérculo. En longitud de tallo, la mejor respuesta se obtuvo con los tratamientos minerales mezclados con materia orgánica (2 y 6 toneladas). La mejor respuesta en rendimiento fue obtenida en la nutrición mineral convencional, mientras que las papas con mayor calibre, se lograron con los tratamientos mineral y mineral mezclado con orgánico. De acuerdo con los resultados, la mejor respuesta en cultivo se puede obtener con la nutrición mineral, en combinación con 6 toneladas de materia orgánica.

Effect of Silymarin on oxidative damage in chicken liver cell membranes / Efecto de la silimarina sobre el daño oxidativo en membranas celulares de hígado de pollo

ABSTRACT In living beings, antioxidants are of vital importance for protection against oxidative damage caused by reactive oxygen species. Silymarin (SM), a plant-derived flavonoid present in the fruits and seeds of milk thistle Silybum marianum (L.) Gaertn., has a recognized hepatoprotective effect. In this work, the in vitro silymarin antioxidant effect on non-enzymatic peroxidation (NEP) in chicken liver mitochondria and microsomes was studied. Oxidative stress in the organelles was induced by subjecting the samples (1 mg of protein) to an ascorbate-Fe++-dependent prooxidant system at 37 °C. Oxidative damage was quantified by chemiluminescence (CL) using a Packard1900 TR liquid scintillation counter (Meriden CT, USA). CL expressed as cpm (counts per minute) was read every 10 minutes to establish the course of peroxidation as a function of time. Likewise, the total cpm value (sum of the readings) was used to compare the inhibitory effect of SM using different concentrations corresponding to 6.25; 12.5, and 25 µg of the active ingredient (silymarin phosphatide) per mg of mitochondrial and microsomal protein. Controls were run simultaneously without the addition of ascorbate. Peroxidation inhibition was dependent on the concentration of SM in the incubation mixture. The results show that a protective effect on induced oxidative damage was found for all concentrations tested.

RESUMEN En los seres vivos, los antioxidantes son de vital importancia para la protección contra el daño oxidativo causado por las especies reactivas del oxígeno. La silimarina (SM), un flavonoide de origen vegetal presente en los frutos y semillas del cardo mariano Silybum marianum (L.) Gaertn., tiene un reconocido efecto hepatoprotector. En este trabajo se estudió el efecto antioxidante in vitro de la silimarina sobre la peroxidación no enzimática (PNE) en mitocondrias y microsomas de hígado de pollo. El estrés oxidativo en los orgánulos se indujo sometiendo las muestras (1 mg de proteína) a un sistema prooxidante dependiente de ascorbato-Fe++ a 37 °C. El daño oxidativo se cuantificó por quimioluminiscencia (QL) utilizando un contador de centelleo líquido Packard 1900 TR (Meriden CT, EE. UU.). QL expresado como cpm (recuentos por minuto) se leyó cada 10 minutos para establecer el curso de la peroxidación en función del tiempo. Asimismo, se usó el valor total de cpm (suma de las lecturas) para comparar el efecto inhibitorio de SM mediante diferentes concentraciones correspondientes a 6,25; 12,5 y 25 µg del ingrediente activo (fosfátido de silimarina) por mg de proteína mitocondrial y microsomal. Los controles se realizaron simultáneamente sin la adición de ascorbato. La inhibición de la peroxidación dependía de la concentración de SM en la mezcla de incubación. Los resultados muestran que para todas las concentraciones probadas se encontró un efecto protector sobre el daño oxidativo inducido

Establishment of patient-derived xenografts of retinoblastoma and choroidal melanoma on the avian chorioallantoic membrane

Purpose: To develop a viable in vivo chorioallantoic membrane (CAM) model to study the growth and invasion of patient?derived retinoblastoma (RB) and choroidal melanoma (CM) xenografts (PDXs). The study utilizes primary tumor samples instead of cancer cell lines, which provides a more authentic representation of tumors due to conserved morphology and heterogeneity. Methods: Fertilized chicken eggs were procured, windowed, and their CAM layers were dropped. On embryonic development day (EDD) 10, freshly cut patient?derived CM and RB tumors were implanted on the CAM layer and the setup was incubated for 7 days. The tumor?embedded CAM layer was harvested on EDD 17, and the extracted tumor samples were subjected to hematoxylin and eosin staining and immunohistochemical analysis to evaluate the extent of tumor invasion. Results: Significant changes in the vascularity around the RB and CM PDXs were observed, indicating an angiogenic environment. The cross?sectional histological view of the tumor implant site revealed the invasion of both the tumors into the CAM mesoderm. Invasion of CM into CAM mesoderm was visualized in the form of pigmented nodules, and that of RB was indicated by synaptophysin and Ki?67 positivity in Immunohistochemistry (IHC). Conclusion: The CAM xenograft model was successfully able to support the growth of CM and RB PDXs and their invasion in CAM, thus presenting as a feasible alternative to mammalian models for studying tumorigenicity and invasiveness of ocular tumors. Moreover, this model can further be utilized to develop personalized medicine by inoculating patient?specific tumors for preclinical drug screening.

Effect of Thermal Treatments on Selected Minerals and Water Soluble Vitamins of Chicken Breast Meat

The study was conducted to ascertain the effect of thermal treatments on selected minerals (Ca, K, Mg, Na, P, Fe and Zn) and water soluble vitamins (B1, B2, B3, B6, B9, B12 and C) contents of chicken breast meat. Industrial skinless chicken breast meat samples were purchased, transported to Bioprocess laboratory in cool conditions, frozen and sliced into dimensions and thawed. The samples were cooked by air frying (AF), baking (BK), deep fat frying (DF) and grilling (GR) at 170, 180 and 1900C for 0, 4, 8 and 12 min for minerals and 0, 8 and 16 min for vitamins. Thereafter, cooked and raw samples were wet acid digested overnight and 5 h digested on a block digester on slowly increased temperature to 1200C, cooled and deionized. The mineral elements were analysed by Optima 4300DV inductivity coupled plasma optical emission spectrometry (ICP-OES) and inductivity coupled plasma mass spectrometry (ICP-MS). These mineral elements were extrapolated through a calibration curve between intensity and concentration, while the vitamins were ascertained by measurement of absorbance of filtrates of the samples dissolved in their respective solvents in the Spectrophotometer against their blank samples at different wavelengths. The results showed that cooking methods decreased significantly (p < 0.05) the mineral elements with an exception of Zn cooked by grilling (GR) that increased by 19.92% and Mg that increased in the cooking methods. The ascending percentage reduction of minerals in cooked chicken breast were Zn, P, K, Fe, Na and Ca. Samples cooked by DF had significantly (p < 0.05) higher percentage reduction of 45.06% in Ca, 27.74% in Na and 18.85% in Zn and higher percentage increases of 14.96% in Mg contents than other methods. Also samples cooked by DF had higher percentage reductions of 55.10%, 37.93%, 37.11%, 34.44% and 30.99% in vitamins B1, C, B2, B9 and B6 Whereas higher percentage reductions of 41.67% and 37.84 % in vitamins B12 and B3 occurred in baking (Bk) and grilling (GR) treated samples. Cooking at 1900C had higher percent reduction in the Ca, Na, Fe, K, P and Zn as well as B1, B12, B2, C, B3, B9 and B6. Cooking methods, temperatures and times decreased significantly (p < 0.05) vitamins and minerals contents of chicken breast meat with an exception of Mg. Samples cooked at 1700C for 4 min and 1700C for 8 min had lower losses of minerals and vitamins compared to similar samples cooked at 1800C and 1900C. The AF cooking method had the least percent reduction of 22.50% than other cooking methods BK (26.88%), DF (36.04%) and GR (30.69%) in vitamin contents.

Microbial Load, Antibiotic Resistant Bacteria and Antibiotic Residues in Broiler Chicken

Chicken meat is highly preferred protein food worldwide. To meet the demand, huge poultry farms are established and using antibiotics as prophylaxis and treatment against the bacterial diseases. Uncontrolled usage of antibiotics has led to development of antibiotic resistance in poultry and antibiotic residues in poultry chicken. Fifty one chicken meat samples were collected from various retail outlets. Antibiotic residues were quantified by HPLC, total microbial load was measured by growth of bacteria on growth medium and antibiotic resistant profile of Escherichia coli, Salmonella spp, Staphylococcus aureus and Campylobacter spp was determined by well diffusion method. Except neomycin, all tested antibiotics were present in the range of 10-978 ppm, the average microbial load was in the range log 10 of 7.32 per gram of chicken sample, E. coli, Salmonella spp, Staphylococcus aureus and Campylobacter spp were resistant to several antibiotics studied. Hence there is a need of appropriate usage of antibiotics in poultry and proper handling of chicken during farming and slaughtering.

The structure and function analysis of bacterial community during aerobic composting of chicken manure / 生物工程学报

In order to determine the changes of bacterial community structure and function in the early, middle and late stage of aerobic composting of chicken manure, high-throughput sequencing and bioinformatics methods were used to determine and analyze the 16S rRNA sequence of samples at different stages of composting. Wayne analysis showed that most of the bacterial OTUs in the three composting stages were the same, and only about 10% of the operational taxonomic units (OTUs) showed stage specificity. The diversity indexes including Ace, Chao1 and Simpson showed a trend of increasing at first, followed by decreasing. However, there was no significant difference among different composting stages (P < 0.05). The dominant bacteria groups in three composting stages were analyzed at the phylum and genus levels. The dominant bacteria phyla at three composting stages were the same, but the abundances were different. LEfSe (line discriminant analysis (LDA) effect size) method was used to analyze the bacterial biological markers with statistical differences among three stages of composting. From the phylum to genus level, there were 49 markers with significant differences among different groups. The markers included 12 species, 13 genera, 12 families, 8 orders, 1 boundary, and 1 phylum. The most biomarkers were detected at early stage while the least biomarkers were detected at late stage. The microbial diversity was analyzed at the functional pathway level. The function diversity was the highest in the early stage of composting. Following the composting, the microbial function was enriched relatively while the diversity decreased. This study provides theoretical support and technical guidance for the regulation of livestock manure aerobic composting process.

Preliminary application of surgical microscope installed with a measuring system in blood vessel measurement in super-microsurgical model of chicken wing / 中华显微外科杂志

Objective:To explore the effect of preliminary application of a surgical microscope with a measuring system in measurement of the outer diameter of blood vessels in the super-microsurgical model of chicken wing.Methods:From November 2022 to January 2023, 12 chicken wing models were established by having the main blood vessels of chicken wings dissected and separated. Outer diameter of blood vessels were measured by the surgical microscope with an installed measuring system and a digital vernier calliper. Results of the 2 measuring methods and measuring time were compared. SPSS 26.0 was used for statistical analysis of the data. The comparison between the 2 measuring methods with continuity data consistent with the homogeneity of normal distribution variance was performed by paired t test, and expressed by Mean ± SD. The difference between the 2 measuring methods was statistically significant ( P<0.05). Results:Results measured by the microscope with a measuring system and a digital vernier calliper were 1.29 mm± 0.08 mm and 1.28 mm± 0.07 mm for chicken wing brachial artery, 1.11 mm± 0.11 mm and 1.09 mm ± 0.11 mm for radial artery, 0.98 mm± 0.09 mm and 0.99 mm± 0.12 mm for ulnar artery, 0.63 mm ± 0.06 mm and 0.64 mm± 0.07 mm for dorsal metacarpal artery, and 0.39 mm± 0.06 mm and 0.40 mm± 0.09 mm for palmar artery, respectively. No significant difference was found between the 2 measuring methods ( P>0.05). The time of measurement for the 2 measuring methods was 5.90 s± 1.12 s and 8.86 s± 1.74 s, respectively. The time for the microscope with a measuring system was less, with statistically significant difference ( P<0.05). Conclusion:It is accurate, convenient and intuitive to use a surgical microscope installed with a measuring system to measure the outer diameter of tinny vessels. It is worth to popularise and apply the surgical microscope equipped with a measuring system in super-microsurgery.

Development and verification of double antibody sandwich ELISA for determination of ovalbumin / 中国生物制品学杂志

@#Objective To develop and verify a double antibody sandwich ELISA detection method for the determination of ovalbumin(OVA),in order to determine the OVA content in influenza vaccines.Methods The rabbit anti-OVA polyclonal antibody was used as coating antibody and HRP labeled rabbit anti-OVA polyclonal antibody as detection antibody to develop a double antibody sandwich ELISA for OVA.The antibody concentration(2,1,0.5 and 0.25 pg/mL) for coating,enzyme-labeled antibody concentration(0.5,0.25 and 0.125 μg/mL),and kinds of blocking reagent(blocking with1% BSA,blocking with 2% BSA,blocking with 1% BSA and 1% sucrose,and blocking with 2% BSA and 2% sucrose,using nonblocking as control) were optimized,and the Cut-off value was determined as the judgment standard.The developed method was verified for the linear range and detection limit,specificity,repeatability and accuracy.Results The optimum detection conditions were as follows:the concentration of coating antibody was 1 μg/mL,the concentration of enzyme-labeled antibody was 0.25 μg/mL;The blocking reagent was 2% BSA and 2% sucrose;The Cut-off value was 0.051 66.The linear range of the method was 5～0.313 ng/mL,and the detection limit was 0.078 ng/mL;It did not react with influenza virus,bovine serum albumin(BSA) and Vero cell supernatant;The intraplate and interplate coefficient of variation(CV) were between 2.562%～13.887% and 4.000%～16.497% respectively;The coincidence rate between the results of this method and the Germany SERAMUN OVA quantitative test kit ranged from 93.79% to 107.05%.Conclusion The developed OVA double antibody sandwich ELISA has good specificity,repeatability,linear range and accuracy with convenience and lower cost,which might be used for the quantitative detection of OVA.

Epidemiological risk factors and phylogenetic affinities of Sarcocystis infecting village chickens and pigs in Peninsular Malaysia

@#The intake of food and water containing the Sarcocystis parasite has been linked to a number of outbreaks worldwide, including Malaysia. Nevertheless, the lack of surveys and epidemiological data on Sarcocystis infections in Malaysia makes it difficult to estimate its occurrence in humans and animals. A cross-sectional study was conducted to determine the prevalence of Sarcocystis and the risk factors associated with infection among village chickens and pigs reared under different farm managements in Peninsular Malaysia. Phylogenetic trees were constructed using partial fragments of the 18S rRNA gene and ITS1 sequences. In the present study, 680 sera samples were collected from village chickens (n=250) and commercial pigs (n=433) and anti-Sarcocystis antibodies were screened using the enzymelinked immunosorbent assays (ELISA) kit. At the animal level, the prevalence of Sarcocystis was 9.2% (95% CI: 5.92-13.48) and at the farm level, it was 64.0% (95% CI: 42.52-82.03) in village chickens. The animal-level seroprevalence of Sarcocystis for pigs was 3.7% (95% CI: 2.13-5.93) and 36.8% (95% CI: 16.29-61.64) at the farm-level. Polymerase Chain Reaction (PCR) was conducted on meat samples from various parts of village chickens (n=250) consisting of brain, heart, lung, and pectoralis muscle tissues, and pork (n=121) consisting of intercostal muscle, diaphragm, and tongue. Sarcocystis DNA was detected in 6.4% (95% CI: 4.60-11.60) of village chicken samples but zero in pork samples. A total of 11 unique Sarcocystis haplotypes were isolated from these tissue samples. Multivariable logistic regression analysis of the putative risk factors showed a statistically significant association between Sarcocystis infection in pigs and uncovered storage of feed. Although no zoonotic Sarcocystis was isolated in this study, we reported the first discovery of S. wenzeli in Malaysia.

Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Aeriscardovia aeriphila, also known as Bifidobacterium aerophilum, was first isolated from the caecal contents of pigs and the faeces of cotton-top tamarin. Bifidobacterium species play important roles in preventing intestinal infections, decreasing cholesterol levels, and stimulating the immune system. In this study, we isolated a strain of bacteria from the duodenal contents of broiler chickens, which was identified as A. aeriphila, and then evaluated the effects of A. aeriphila on growth performance, antioxidant functions, immune functions, and gut microbiota in commercial broiler chickens. Chickens were orally gavaged with A. aeriphila (1×109 CFU/mL) for 21 d. The results showed that A. aeriphila treatment significantly increased the average daily gain and reduced the feed conversion ratio (P<0.001). The levels of serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) were significantly increased following A. aeriphila treatment (P<0.05). Blood urea nitrogen and aspartate aminotransferase levels were decreased, whereas glucose and creatinine levels increased as a result of A. aeriphila treatment. Furthermore, the levels of serum antioxidant enzymes, including catalase (P<0.01), superoxide dismutase (P<0.001), and glutathione peroxidase (P<0.05), and total antioxidant capacity (P<0.05) were enhanced following A. aeriphila treatment. A. aeriphila treatment significantly increased the levels of serum immunoglobulin A (IgA) (P<0.05), IgG (P<0.01), IgM (P<0.05), interleukin-1 (IL-1) (P<0.05), IL-4 (P<0.05), and IL-10 (P<0.05). The broiler chickens in the A. aeriphila group had higher secretory IgA (SIgA) levels in the duodenum (P<0.01), jejunum (P<0.001), and cecum (P<0.001) than those in the control group. The messenger RNA (mRNA) relative expression levels of IL-10 (P<0.05) and IL-4 (P<0.001) in the intestinal mucosa of chickens were increased, while nuclear factor-‍κB (NF‍-‍κB) (P<0.001) expression was decreased in the A. aeriphila group compared to the control group. Phylum-level analysis revealed Firmicutes as the main phylum, followed by Bacteroidetes, in both groups. The data also found that Phascolarctobacterium and Barnesiella were increased in A. aeriphila-treated group. In conclusion, oral administration of A. aeriphila could improve the growth performance, serum antioxidant capacity, immune modulation, and gut health of broilers. Our findings may provide important information for the application of A. aeriphila in poultry production.

Expression of Cyclooxygenase-2 in Patients With Snow-White Sign of Advanced Colorectal Adenomas / 中国医学科学院学报

Objective To analyze the expression of cyclooxygenase-2 (COX-2) in the patients with snow-white sign of advanced colorectal adenoma (ACA) and explore its clinical significance.Method Western blotting was employed to determine the expression of COX-2 in the adenoma tissue and the normal tissue adjacent to the adenoma tissue (>5 cm away from the distal end of the adenoma tissue) of 40 ACA patients with snow-white sign and 40 ACA patients without snow-white sign.Results The appearance of snow-white sign in ACA patients was associated with patient age (P=0.001) and not associated with sex,smoking history,drinking history,ethnic groups,family history of colorectal cancer,abdominal pain,diarrhea,constipation,fecal occult blood,or tumor markers (all P>0.05).Snow-white sign mainly appeared in the ACA patients with multiple adenomas (P=0.004),large adenomas (P=0.006),adenomas in distal colon (P=0.015),protruding polyps (P=0.044),and late-stage pathology (P=0.010).The occurrence of snow-white sign showed no difference in the ACA patients with different results of Japan NBI Expert Team classification (P=0.502).The expression of COX-2 in the adenoma tissue was higher than that in the adjacent normal tissue in the patients with and without snow-white sign (P<0.001,P=0.004).The patients with snow-white sign had higher expression of COX-2 protein in the adenoma tissue than the patients without snow-white sign (P=0.001).The expression of COX-2 protein in the adjacent healthy tissue had no significant difference between the patients with and without snow-white sign (P=0.603).Conclusions Snow-white sign is more like to appear in the ACA patients with young age,multiple and large adenomas,adenomas in distal colon,protruding polyps,and late-stage pathology.Moreover,the expression of COX-2 in the ACA patients with snow-white sign is significantly higher than that in the ACA patients without snow-white sign.The adults with snow-white sign are prone to cancerization than those without snow-white sign.

Monitoring results of veterinary drug residues in chicken and eggs in Xinjiang in 2019 - 2020 / 公共卫生与预防医学

Objective To know the residues of 13 veterinary drug residues in chicken and eggs foods in some areas in Xinjiang. Methods A total of 170 chicken and egg samples were randomly selected from supermarkets and farmers' markets in seven cities in Xinjiang. Eleven quinolone antibiotics, two tetracycline antibiotics, ribavirin and metronidazole were examined for veterinary drug residues using liquid chromatography tandem mass spectrometry (LC-MS/MS) . Results The overall detection rate of veterinary drug residues in eggs and chicken were 20%（18/90）and11.25%（9/80）. The overall over-standard rate were 18.89%（17/90）and 0（0/80）. Veterinary drug residues in chicken are heavier than eggs. Veterinary drugs were detected and over-standarded in all seven cities in Xinjiang monitored. Conclusion The veterinary drug contamination in chicken and eggs in Xinjiang is relatively serious. It is recommended to strengthen the standardization of production and supervision to ensure food safety.

Mathematical models to describe the growth curves of Vietnamese Ri chicken / Modelos matemáticos para descrever as curvas de crescimento do frango Ri vietnamita

Ri chicken is the most popular backyard chicken breed in Vietnam, but little is known about the growth curve of this breed. This study compared the performances of models with three parameters (Gompertz, Brody, and Logistic) and models containing four parameters (Richards, Bridges, and Janoschek) for describing the growth of Ri chicken. The bodyweight of Ri chicken was recorded weekly from week 1 to week 19. Growth models were fitted using minpack.lm package in R software and Akaike’s information criterion (AIC), Bayesian information criterion (BIC), and root mean square error (RMSE) were used for model comparison. Based on these criteria, the models having four parameters showed better performance than the ones with three parameters, and the Richards model was the best one for males and females. The lowest and highest value of asymmetric weights (α) were obtained by Bridges and Brody models for each of sexes, respectively. Age and weight estimated by the Richard model were 8.46 and 7.51 weeks and 696.88 and 487.58 g for males and for females, respectively. Differences in the growth curves were observed between males and female chicken. Overall, the results suggested using the Richards model for describing the growth curve of Ri chickens. Further studies on the genetics and genomics of the obtained growth parameters are required before using them for the genetic improvement of Ri chickens.

O frango Ri é a raça de frango de quintal mais popular do Vietnã, mas pouco se sabe sobre a curva de crescimento dessa raça. Este estudo comparou o desempenho de modelos com três parâmetros (Gompertz, Brody e Logistic) e modelos contendo quatro parâmetros (Richards, Bridges e Janoschek) para descrever o crescimento do frango Ri. O peso corporal do frango Ri foi registrado semanalmente da semana 1 à semana 19. Os modelos de crescimento foram ajustados usando o pacote minpack.lm no software R e o critério de informação de Akaike (AIC); critério de informação bayesiano (BIC) e erro quadrático médio (RMSE) foram usados para comparação de modelos. Com base nesses critérios, os modelos com quatro parâmetros apresentaram melhor desempenho do que os com três parâmetros, sendo o modelo de Richards o melhor para homens e mulheres. O menor e o maior valor dos pesos assimétricos (α) foram obtidos pelos modelos Bridges e Brody para cada um dos sexos, respectivamente. A idade e o peso estimados pelo modelo de Richard foram de 8,46 e 7,51 semanas e 696,88 e 487,58 g para homens e mulheres, respectivamente. Diferenças nas curvas de crescimento foram observadas entre frangos machos e fêmeas. No geral, os resultados sugeriram o uso do modelo de Richards para descrever a curva de crescimento de frangos Ri. Mais estudos sobre a genética e genômica dos parâmetros de crescimento obtidos são necessários antes de usá-los para o melhoramento genético de frangos Ri.

Mathematical models to describe the growth curves of Vietnamese Ri chicken

Abstract Ri chicken is the most popular backyard chicken breed in Vietnam, but little is known about the growth curve of this breed. This study compared the performances of models with three parameters (Gompertz, Brody, and Logistic) and models containing four parameters (Richards, Bridges, and Janoschek) for describing the growth of Ri chicken. The bodyweight of Ri chicken was recorded weekly from week 1 to week 19. Growth models were fitted using minpack.lm package in R software and Akaikes information criterion (AIC), Bayesian information criterion (BIC), and root mean square error (RMSE) were used for model comparison. Based on these criteria, the models having four parameters showed better performance than the ones with three parameters, and the Richards model was the best one for males and females. The lowest and highest value of asymmetric weights () were obtained by Bridges and Brody models for each of sexes, respectively. Age and weight estimated by the Richard model were 8.46 and 7.51 weeks and 696.88 and 487.58 g for males and for females, respectively. Differences in the growth curves were observed between males and female chicken. Overall, the results suggested using the Richards model for describing the growth curve of Ri chickens. Further studies on the genetics and genomics of the obtained growth parameters are required before using them for the genetic improvement of Ri chickens.

Resumo O frango Ri é a raça de frango de quintal mais popular do Vietnã, mas pouco se sabe sobre a curva de crescimento dessa raça. Este estudo comparou o desempenho de modelos com três parâmetros (Gompertz, Brody e Logistic) e modelos contendo quatro parâmetros (Richards, Bridges e Janoschek) para descrever o crescimento do frango Ri. O peso corporal do frango Ri foi registrado semanalmente da semana 1 à semana 19. Os modelos de crescimento foram ajustados usando o pacote minpack.lm no software R e o critério de informação de Akaike (AIC); critério de informação bayesiano (BIC) e erro quadrático médio (RMSE) foram usados para comparação de modelos. Com base nesses critérios, os modelos com quatro parâmetros apresentaram melhor desempenho do que os com três parâmetros, sendo o modelo de Richards o melhor para homens e mulheres. O menor e o maior valor dos pesos assimétricos () foram obtidos pelos modelos Bridges e Brody para cada um dos sexos, respectivamente. A idade e o peso estimados pelo modelo de Richard foram de 8,46 e 7,51 semanas e 696,88 e 487,58 g para homens e mulheres, respectivamente. Diferenças nas curvas de crescimento foram observadas entre frangos machos e fêmeas. No geral, os resultados sugeriram o uso do modelo de Richards para descrever a curva de crescimento de frangos Ri. Mais estudos sobre a genética e genômica dos parâmetros de crescimento obtidos são necessários antes de usá-los para o melhoramento genético de frangos Ri.

Mathematical models to describe the growth curves of Vietnamese Ri chicken / Modelos matemáticos para descrever as curvas de crescimento do frango Ri vietnamita

Abstract Ri chicken is the most popular backyard chicken breed in Vietnam, but little is known about the growth curve of this breed. This study compared the performances of models with three parameters (Gompertz, Brody, and Logistic) and models containing four parameters (Richards, Bridges, and Janoschek) for describing the growth of Ri chicken. The bodyweight of Ri chicken was recorded weekly from week 1 to week 19. Growth models were fitted using minpack.lm package in R software and Akaike's information criterion (AIC), Bayesian information criterion (BIC), and root mean square error (RMSE) were used for model comparison. Based on these criteria, the models having four parameters showed better performance than the ones with three parameters, and the Richards model was the best one for males and females. The lowest and highest value of asymmetric weights (α) were obtained by Bridges and Brody models for each of sexes, respectively. Age and weight estimated by the Richard model were 8.46 and 7.51 weeks and 696.88 and 487.58 g for males and for females, respectively. Differences in the growth curves were observed between males and female chicken. Overall, the results suggested using the Richards model for describing the growth curve of Ri chickens. Further studies on the genetics and genomics of the obtained growth parameters are required before using them for the genetic improvement of Ri chickens.

Resumo O frango Ri é a raça de frango de quintal mais popular do Vietnã, mas pouco se sabe sobre a curva de crescimento dessa raça. Este estudo comparou o desempenho de modelos com três parâmetros (Gompertz, Brody e Logistic) e modelos contendo quatro parâmetros (Richards, Bridges e Janoschek) para descrever o crescimento do frango Ri. O peso corporal do frango Ri foi registrado semanalmente da semana 1 à semana 19. Os modelos de crescimento foram ajustados usando o pacote minpack.lm no software R e o critério de informação de Akaike (AIC); critério de informação bayesiano (BIC) e erro quadrático médio (RMSE) foram usados ​​para comparação de modelos. Com base nesses critérios, os modelos com quatro parâmetros apresentaram melhor desempenho do que os com três parâmetros, sendo o modelo de Richards o melhor para homens e mulheres. O menor e o maior valor dos pesos assimétricos (α) foram obtidos pelos modelos Bridges e Brody para cada um dos sexos, respectivamente. A idade e o peso estimados pelo modelo de Richard foram de 8,46 e 7,51 semanas e 696,88 e 487,58 g para homens e mulheres, respectivamente. Diferenças nas curvas de crescimento foram observadas entre frangos machos e fêmeas. No geral, os resultados sugeriram o uso do modelo de Richards para descrever a curva de crescimento de frangos Ri. Mais estudos sobre a genética e genômica dos parâmetros de crescimento obtidos são necessários antes de usá-los para o melhoramento genético de frangos Ri.

Effects of virginiamycin and galbanum (Ferula gummosa boiss) on performance, carcass traits, immune system and blood parameters of broiler chickens / Efectos de la virginiamicina y el gálbano (Ferula gummosa boiss) sobre la producción, características de la canal, sistema inmune y parámetros sanguíneos en pollos de engorde / Efeitos na adição na dieta do virginiamicina e do gálbano (Ferula gummosa boiss) no desempenho, características da carcaça, sistema imunológico e fatores sanguíneos de frangos de corte

Abstract Background: Some medicinal plants can stimulate growth in poultry. Objective: To compare the effects of dietary addition of virginiamycin antibiotic and galbanum plant (Ferula gummosa) on the performance, carcass characteristics, immune system, and blood factors of broiler chickens. Methods: A total of 250 one-day-old male Ross 308 broiler chickens were randomly assigned to five treatments with five replicates (10 birds per replicate). The treatments were: control (basal diet), virginiamycin (basal diet plus 0.1 g virginiamycin/kg of diet) or one of three levels of galbanum powder (2.5, 5, and 10 g galbanum/kg of diet). Results: Body weight gain, feed intake and feed conversion ratio improved in the groups containing galbanum compared to the other groups (p<0.05). The percentage of liver, heart and abdominal fat decreased in the treatments containing 5 and 10 g galbanum compared to the control (p<0.05). Spleen weight and antibody titers against Newcastle disease and SRBC were significantly increased in the treatment containing 10 g of galbanum compared to the other treatments (p<0.05). The addition of galbanum powder resulted in a significant decrease in serum cholesterol, triacylglycerol, and LDL (p<0.05). Additionally, galbanum increased serum total protein, albumin, globulin, and HDL (p<0.05). Conclusion: Galbanum improves performance and the immune system of broiler chickens, and has similar growth promoting effects as virginiamycin.

Resumen Antecedentes: Algunas plantas medicinales pueden estimular el crecimiento de las aves. Objetivo: Comparar los efectos de añadir virginiamicina y gálbano (Ferula gummosa) a la dieta sobre la producción, características de la canal, sistema inmune y parámetros sanguíneos de pollos de engorde. Métodos: Un total de 250 pollos machos de engorde Ross 308 con un día de vida se asignaron aleatoriamente a cinco tratamientos (5 réplicas y 10 aves por réplica). Los tratamientos fueron: control (dieta basal), virginiamicina (0,1 g virginiamicina/kg dieta) y tres niveles de gálbano en polvo (2,5, 5 y 10 g galvano/kg dieta). Resultados: La ganancia de peso, el consumo de alimento y el índice de conversión mejoraron en los tratamientos que contenían gálbano comparados con los otros grupos (p<0,05). los porcentajes de grasa hepática, cardíaca y abdominal se redujeron significativamente en los tratamientos que contenían 5 y 10 g/kg de gálbano comparados con el control (p<0,05). El peso del bazo y la producción de anticuerpos contra las enfermedades de Newcastle y SRBC aumentaron significativamente en el tratamiento con 10 g/kg de gálbano en comparación con los otros tratamientos (p<0,05). La adición de gálbano resultó en una disminución significativa de los niveles de colesterol, triglicéridos y LDL (p<0,05), mientras que los niveles séricos de proteína total, albúmina, globulina y HDL aumentaron significativamente (p<0,05). Conclusión: Los resultados mostraron que el gálbano mejora la producción y refuerza el sistema inmune en pollos de engorde, y promueve el crecimiento de forma similar a la virginiamicina.

Resumo Antecedentes: Algumas plantas medicinais podem estimular o crescimento dos pássaros. Objectivo: Comparar os efeitos da adição na dieta do antibiótico virginiamicina e do gálbano (Ferula gummosa) no desempenho, características de carcaça, sistema imunológico e fatores sanguíneos de frangos de corte. Métodos: Um total de 250 frangos de corte, machos Ross 308, de um dia de idade foram aleatoriamente divididos em 5 tratamentos e 5 repetições (10 aves por repetição). Os tratamentos foram: grupo controle (dieta base), grupo com adição de antibiótico virginiamicina (0,1 g virginiamicina/kg de dieta) e três outros grupos com diferentes níveis gálbano em pó (2,5, 5 e 10 g galvano/kg de dieta). Resultados: O ganho de peso corporal, o consumo de ração e a taxa de conversão melhoraram nos grupos contendo 5 e 10 gramas de gálbano em comparação com os outros grupos (p<0,05). As diferenças no percentual de gordura hepática, cardíaca e abdominal diminuíram significativamente nos tratamentos contendo 5 e 10 g de gálbano em comparação ao controle (p<0,05). O peso do baço e os títulos de anticorpos gerados contra a doença de Newcastle e SRBC foram significativamente aumentados no tratamento contendo 10 gramas de gálbano em comparação com os outros tratamentos (p<0,05). A adição de gálbano em pó resultou em diminuição significativa do colesterol sérico, triacilglicerol e LDL (p<0,05) e, por outro lado, aumento significativo da proteína sérica total, albumina, globulina e HDL (p<0,05). Conclusões: Os resultados mostraram que a erva medicinal gálbano melhora o desempenho e estimula o sistema imunológico em frangos de corte e pode ser considerada um factor de crescimento em frangos de corte em comparação com o antibiótico virginiamicina.