الملخص
Abstract Background Histopathology can be crucial for diagnosis of inflammatory nail diseases. Longitudinal excision and punch biopsies are the most used techniques to obtain the tissue sample. However, there is a low clinical-histopathological correlation, besides the risk of nail dystrophy. Tangential excision biopsy (TB) is a well-established technique for the investigation of longitudinal melanonychia. TB could also be used to evaluate diseases in which histopathological changes are superficial, as in psoriasis. Objective To study the value of TB in the histopathological diagnosis of nail psoriasis. Methods This is a prospective and descriptive study of the clinical-histopathological findings of samples from the nail bed or matrix and nail plate of 13 patients with clinical suspicion of nail psoriasis. Biopsies were obtained through partial nail avulsion and TB. Results In nine patients, the hypothesis of psoriasis was confirmed by histopathology; in one, the criteria for diagnosing nail lichen planus were fulfilled. The tissue sample of only one patient did not reach the dermal papillae, and, in four of 13 patients, the adventitial dermis was not sampled. No patient developed onychodystrophy after the procedure. Study limitations In three patients, the clinical and, consequently, histopathological nail changes were subtle. Also, in one patient's TB didn't sample the dermal papillae. Conclusions TB is a good option to assist in the histopathological diagnosis of nail psoriasis, especially when appropriate clinical elements are combined. Using this technique, larger and thinner samples, short postoperative recovery time, and low risk of onychodystrophy are obtained.
الملخص
Background: Obstetric hysterectomy (OH) is the removal of the uterus at the time of caesarean section, following vaginal delivery or within the puerperium period. Despite developments in the conservative medical &surgical management of OH, it remains a lifesaving technique for the management of persistent hemorrhage refractory to conservative control. Histopathological examination of the specimen is necessary as it determines the cause of OH and confirms the clinical and radiological indications.Methods: This was cross-sectional observational study conducted from January 2017 to December 2022. It was conducted on 62 obstetric hysterectomy specimens.Results: The proportion of OH was 6.05% out of a total 1024 hysterectomies. The age range of the study population was 20-45 years with a mean of 30.35�25 years. The trend towards OH increased with an increase in LSCS and it has been considered as an underlying high-risk factor for OH. The chief morphological abnormalities found were placenta accreta, placenta previa, placenta increta, placenta percreta, and retained placenta, followed by subinvolution. The radiological and morphological findings in 21 cases showed a statistically significant association.Conclusions: Abnormal placentation is the predominant cause of obstetric hysterectomy. The association between the rising CS rate and incidence of obstetric hysterectomy with a history of CS is attributable mostly to the occurrence of morbidly adherent placenta/placenta increta These abnormalities are amenable to early diagnosis by imaging methods.
الملخص
Background: Formalin 10% is a fixative agent used in pathology laboratories. Formaldehyde released from formalin is a strong irritant and a carcinogen. The lab personnel are exposed to 10% formalin preserved surgical and post-mortem tissue samples during the visual examination and grossing. The present study aims to assess the exposure to formaldehyde in a histopathology laboratory unit as well as the effectiveness of existing engineering/ventilation systems. Methods: This is a cross-sectional study. Formalin levels were measured using portable air quality/pollution meter which measures formaldehyde (HCHO) in terms of mg/m3 in the morning, noon, and evening in different areas for one month. Areas of rooms and ventilation were mapped. The level of formalin was noted before, during, and after the grossing procedure and compared with the reference values given in the Occupational Safety and Health Administration (OSHA) and World Health Organisation (WHO). Results: Formaldehyde concentration ranged from 0.005 to 0.48 ppm (parts per million) in the grossing room and 0.002-0.010 ppm in the museum. Formaldehyde levels were highest in the morning and during grossing without using exhaust/ventilation and the levels reached minimum value within 15-20 minutes of switching on the existing control methods (exhaust fan of grossing station and opening of window panes). Conclusions: Formalin from the histology laboratories cannot be removed entirely but can be reduced sufficiently to lessen the risks to health by educating lab personnel and adopting appropriate control techniques.
الملخص
Background: First of all, globally, ovarian tumors are becoming a more common source of morbidity and death. The goal of the current investigation was to ascertain the incidence of different ovarian tumor histological and morphological variants in a tertiary healthcare center in southern India.Methods: The study, which took place in a tertiary healthcare facility in Chennai, involved 89 ovary specimens that were obtained over the course of 18 months (from August 2021 to December 2022) from patients in the obstetrics and gynecology department. The specimens underwent both histological and critical gross examination. Standard H&E-stained paraffin slices were observed. After being organized on proforma, the histology results were examined.Results: A total of 89 patients, ranging in age from 16 to 90, were enrolled in the study. The majority of patients (30.3%) were in the age range of 31 to 40 years. The majority of ovarian tumors were benign, accounting for 58 (65.2%) of the total, while malignant tumors accounted for 25 (28.1%). The most prevalent type of tumors, according to the World Health Organization (WHO) classification, were surface epithelial tumors (76.12%), followed by germ cell tumors (18.46%). In the current investigation, one case of metastatic tumors to the ovaries was also identified. Serous cystadenoma 29 (32.6%) was found to be the most prevalent subtype among the many known subtypes of ovarian tumors, followed by papillary serous carcinoma 14 (15.7%).Conclusions: Compared to malignant or borderline tumors, benign ovarian tumors are more common. Surface epithelial tumors are the most prevalent histological subtype of ovarian cancers, followed by germ cell tumors. It's critical to distinguish between benign and malignant tumors in order to ensure appropriate treatment and healing. Future research of this kind with a larger sample size is advised.
الملخص
Xanthogranulomatous oophoritis is a rare, peculiar, non-neoplastic, chronic inflammatory pathology that can mimic ovarian mass. Affected organ shows replacement of normal tissue with foamy histiocytes, multinucleate giant cells, neutrophils, fibroblasts, plasma cells and areas of necrosis within. Its aetiology is still unknown, and the final diagnosis is usually made through histopathological examination. The present case report describes an unusual presentation of simultaneous occurrence of Xanthogranulomatous oophoritis and fibrothecoma masquerading as bilateral tubo-ovarian masses in a post-menopausal female. Despite initial assumptions and suspicions of tubo-ovarian abscesses, surgical exploration revealed an unexpected pathology. Histopathological evaluation confirmed the diagnosis, emphasising the importance of vigilant and comprehensive assessment in atypical presentations.
الملخص
Introducción: las neoplasias malignas en cavidad oral representan de 3-5% de todas las neoplasias, el carcinoma de células escamosas representa 90%, es la neoplasia más frecuente. Objetivo: identificar la frecuencia de neoplasias malignas diagnosticadas histopatológicamente en 2,042 casos consecutivos en la cavidad oral, en un servicio patológico privado, clasificándolas por edad, sexo y localización. Material y métodos: estudio transversal de un muestreo por conveniencia en un periodo que data de enero de 2017 a diciembre de 2018. Resultados: la prevalencia de neoplasias malignas fue de 6.06% (124/2042). Conclusiones: las neoplasias malignas afectan principalmente a personas mayores de 40 años con predilección por el sexo masculino, probablemente porque las mujeres acuden con mayor frecuencia a evaluaciones médicas (AU)
Introduction: malignant neoplasms in oral cavity represent 3 to 5% of all the neoplasms where squamous cell carcinoma is the most frequent with a representation rate of 90%. Objective: identify the frequency of malignant neoplasms diagnosed histopathologically in 2,042 consecutive cases in the oral cavity in a private oral pathology service, classifying them by age, sex and location. Material and methods: cross-sectional study of a sampling for convenience from the periods of January 2017 to December 2018. Results: the prevalence of malignant neoplasms was 6.06% (124/2042). Conclusions: malignant neoplasms mainly affect people over 40; with a predilection for the male sex, probably because women attend medical evaluations more frequently (AU)
الموضوعات
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Mouth Neoplasms/diagnosis , Mouth Neoplasms/epidemiology , Carcinoma, Squamous Cell/epidemiology , Data Interpretation, Statistical , Age Factors , Age and Sex Distribution , Hospitals, Military/statistics & numerical data , Mexico/epidemiologyالملخص
Background: Rifampicin and Isoniazid are two main medicinal drugs used as regimen in the treatment of Tuberculosis. These drugs induce hepatotoxicity. Liv-52, a polyherbal formulation has been shown to have clinical use in the treatment of liver disorders. The aim of this study was to investigate the histopathological and biochemical effects of Liv-52 on INH and RIF induced hepatotoxicity. Methods: Adult albino rats weighing 150g to 250g were used. A total of 24 rats were randomly assigned into 4 groups of 6 rats each. Group 1 served as negative control. Hepatotoxicity was achieved by administering 50 mg/kg/day of RIF and INH each as positive control. Hepatoprotective effect was determined by administering Liv-52 concurrently with positive control. Low dose Liv-52 and high dose Liv-52 was administered at (155 mg/kg/day and 207 mg/kg/day) respectively, concurrently with RIF and INH at (50 mg/kg) each orally daily. After 21 days, the albino rats were sacrificed humanely, liver harvested and blood samples taken for estimation of liver serum biomarkers. The livers were processed and stained with Haematoxylin and Eosin for histological examination. Significance levels of (p?0.05). Results: The three selected liver biochemical parameters (ALT, AST and ALP) significantly increased in positive control group relative to negative control. The hepatoprotective groups (especially the HD Liv-52 group) showed significant reduction in the biochemical parameters. The liver histopathological results confirmed the above findings. Conclusion: High dose Liv-52 significantly prevents hepatotoxicity induced by antitubercular therapy by inhibiting rise liver biochemical parameters and also ameliorating the deranged liver histomorphological features.
الملخص
Background: Aim was to compare histological diagnosis of differently stained endometrial tissue on chromohysteroscopy.Methods: A total of 80 patients diagnosed with AUB and satisfying the study design were included in the study. Hysteroscopy followed by chromohysteroscopy was done using 1% methylene blue. Staining patterns were observed and guided biopsies were taken from differently stained areas and sent for histopathology.Results: On chromohysteroscopy, out of the 80 participants, 53 (66.3%) had focal staining and 27 (33.7%) had diffuse staining. The overall sensitivity, specificity, PPV and NPV of TVS in diagnosing uterine abnormalities was 51.7%, 45.1%, 34.9%, 62.2% respectively. The overall sensitivity, specificity, PPV and NPV for hysteroscopy were 96.6%, 41.2%, 48.3%, 95.5% respectively. The indices for chromohysteroscopy were as follows: sensitivity-69% for focal and 31% for diffuse staining, specificity-49.0% for focal staining and 69.7% for diffuse staining, PPV-43.5% for focal and 33.3% for diffuse staining, NPV-73.5% for focal staining and 62.3% for diffuse staining.Conclusions: The idea of staining of endometrium and taking a guided biopsy is exciting and is undoubtedly, better than a blind sampling. However, subjecting all the patient of AUB to chromohysteroscopy in order to find a major histopathological difference is questionable and needs larger trials to reach to concrete decision.
الملخص
Objetivo: Reportar los hallazgos anatomopatológicos de las apendicectomías profilácticas en el Hospital Clínico de la Fuerza Aérea de Chile (FACh) y compararlos con los estudios reportados en la literatura. Material y Método: Análisis retrospectivo, descriptivo de los pacientes sometidos a apendicectomía laparoscópica profiláctica entre los años 2013 y 2017 en el Hospital FACh, y sus resultados anatomopatológicos de la pieza operatoria, según registro clínico y reportes de laboratorio patológico. Resultados: Se rescataron un total de 200 muestras de pacientes sometidos a apendicectomía. El 80% resultó sin hallazgos patológicos, mientras que 13,5% obliteración fibrosa, y 2,5% en apendicitis aguda en distinta etapa. Se evidenció una muestra de 0,5% de neoplasia maligna. Discusión: El porcentaje de apéndices no patológicos obtenidos se correlaciona con resultados expuestos en la literatura sobre apendicectomías incidentales. Por su parte, los hallazgos de malignidad están presentes en todos los reportes revisados, con una tasa relativa baja variable. Existe el debate si todos los apéndices deben ser estudiados. Conclusiones: No existen estudios similares al presente, sin embargo, es comparable a los reportes de apendicectomía incidental. Pareciera ser indispensable el estudio histopatológico por el riesgo de prevalencia de malignidad.
Objective: To report anatomopathological results of the prophylactic appendectomies in the Hospital Clínico de la Fuerza Aérea de Chile (FACh) and to compare them with existing studies. Material and Method: Descriptive, retrospective analysis of patients that underwent prophylactic appendectomies between 2013 and 2017 in the Hospital FACh and their anatomopathological results, according to clinical registries and pathology reports. Results: A total of 200 samples obtained from patients that underwent appendectomy were rescued. 80% resulted with no pathological findings, 13,5% had fibrous obliteration, and 2,5% showed acute appendicitis in different stages. Malignancy was found in 0,5% of the samples. Discussion: The percentage of non-pathological appendices correlates with the results reported in the literature. On the other hand, malignancy is present in all the reviewed reports, with a variable low relative rate. There is a debate on whether all appendixes should be studied. Conclusion: There are no studies like this one, however, our results are comparable to the reports of incidental appendectomies. Histopathological analysis seems to be essential due to the risk of prevalence of malignancy.
الملخص
Breast tuberculosis is a rare disease even in endemic regions. It often presents in young females as a breast lump and is confused with other benign and malignant lesions. We presented a case of 47-year-old woman, initially diagnosed with a breast carcinoma based on clinical examination and radiological imaging. However, breast biopsy with histopathological analysis revealed breast tuberculosis. Anti-tubercular chemotherapy was used during six months with good evolution. Given low specificity of clinical and radiological findings of mammary tuberculosis, histopathological study of any breast nodule is mandatory to exclude this rare entity and avoid unnecessary surgical procedure.
الملخص
Xanthogranulomatous inflammation, a form of destructive chronic inflammation is a rare occurrence in female genital tract, especially ovary and fallopian tube. Its clinical and radiological presentation mimics ovarian malignancy which poses a diagnostic dilemma at times. Histopathology plays a pivotal role in diagnosis of this entity. We described seven cases in total, out of which two cases showed xanthogranulomatous oophoritis and five had both xanthogranulomatous oophoritis and salpingitis, all diagnosed on histopathology. Majority of the patients had abdominal pain, fever and adnexal mass on examination. Most women were treated based on the working diagnosis of malignancy or severe pelvic inflammatory disease. Histopathology remains the gold standard for diagnosis in all these cases, and with the aid of clinical and radiological details helps in ruling out other differential diagnosis.
الملخص
Background: Majority women encounter abnormal uterine bleeding in their life where the causes are not limited to cancer even unknown. The actual cause has not been studied in north-east part of India. This data is scarce in State of Tripura. The present study aims to find out histomorphological patterns of AUB reported in AGMC & GBP Hospital in a calendar year. Methods: A cross sectional study was conducted over 2 calendar year from 2021 to 2022 where the cases of AUB irrespective of age, who had undergone endometrial biopsy, endometrial curettage and hysterectomy in Obstetrics and Gynaecology department, AGMC were examined histomorphologically in the dept of Pathology, AGMC. Data were collected in predesigned proforma and analysed using SPSS 21.0 and expressed in both descriptive and inferential statistics. Ethical approval was taken. Results: Total 220 cases of AUB specimens were examined. Among the reported AUB cases, the mean age is 42.5�42 year. Overall, 16.81% of the cases of AUB shows normal tissue pattern and 83.19% with abnormal endometrial tissue. Out of 220 sample of AUB, the commonest cause was leiomyoma (20.45%) followed by endometrial hyperplasia (17.27%), adenomyosis (14.54%), mixed tumour lesion (9.09%), endometrial carcinoma (4.54%) and 1.35% shows cervical cancer. Conclusions: Study concluded that In Tripura, majority AUB cases are due to leiomyoma, endometrial hyperplasia, adenomyosis and mixed inflammation indicating proper screening during reproductive stage of the women.
الملخص
Background: Terminalia catappa nut possesses antioxidant and anticancer properties, but its effects on leukemia are unclear. This study investigates the effects of n-hexane extract of Terminalia catappa nut (TCN) on some hematological parameters, oxidative stress markers, and bone/spleen histopathology in a Wistar rat model of benzene-induced leukemia. Methods: Leukemia was induced in Wistar rats with 0.2 ml/kg Benzene solution and treated with 200, 400 or 800 mg/kg/day of Terminalia catappa nut extract (TCN) for 42 days and with 5-fluorouracil (20 mg/kg) via intraperitoneal injection twice a week for 6 weeks. Hematological parameters, antioxidant markers, and bone and spleen histology were analysed. Results: All TCN doses significantly lowered elevated WBCs by 32-53% and normalized RBC parameters compared to leukemic controls, mitigating cancer-induced anemia. TCN also exhibited potent antioxidant effects by enhancing SOD, GSH, and catalase while reducing MDA versus untreated rats. Bone marrow analysis revealed TCN conferred dose-dependent benefits on cellularity and architecture, reducing myeloid blasts and leukocyte infiltration. A near-normal bone microarchitecture was attained with the highest TCN dose. Similarly, TCN elicited marked improvements in splenic cytoarchitecture and attenuation of hypercellularity, lymphocytic infiltration and megakaryocytes compared to leukemic controls in a dose-dependent manner. Conclusions: Terminalia catappa nut extract demonstrated anti-leukemic, haematopoietic, antioxidant, and organ protective effects in leukemic Wistar rats induced with benzene solution, supporting its potential as an adjuvant therapeutic agent.
الملخص
Background: Assessment of postmenopausal women with red flag signs like postmenopausal bleeding/ endometrial thickness ?5 mm is needed for early detection of any pathology. Early-stage endometrial cancer detection through office hysteroscopy is one of crucial modality for effective management. Objective was to assess the outcome of office hysteroscopy in postmenopausal women with red flag sign and correlated with histopathology findings. Methods: A prospective observational study at a single facility in Northern Ireland involving 122 post-menopausal women aged ?45 years subjected to AUB/increased endometrial thickness. Advance diagnostic examination by office hysteroscopy followed by histopathology to detect and rule out endometrial cancer and related ailments.Results: The mean age of the study population was 63.07±10.317 SD years and 91.8% were over-weight. 71.3% women had endometrial thickness <5 mm. Hysteroscopic reported for 8.2% (10 cases) endometrial cancers having diagnostic accuracy of 98.36%. The histological and hysteroscopic diagnoses were having significantly similar outcomes. Office hysteroscopy has 100% specificity and positive predictive value in detecting normal endometrium whereas 98.25% specificity and 100% negative predictive value in case for cancer detection.Conclusions: In Irish women, office hysteroscopy proved helpful in determining the pathologies that cause postmenopausal bleeding. Correlating hysteroscopic and histological findings with red flag indications should be the focus of future explorations.
الملخص
Objectives@#In the Philippines, there has been a lack of information on the concordance between classifications of Hansen’s disease or leprosy clinically, histopathologically, and with AFS results. The study ultimately aimed to determine the concordance between the clinical diagnosis, histopathological results, and AFS results of patients with leprosy seen at the Dr. Jose N. Rodriguez Memorial Hospital and Sanitarium (DJNRMHS). @*Methods@#This is a descriptive, retrospective, single-center study conducted at the DJNRMHS, a tertiary government hospital and one of the last remaining sanitaria in the country located in northern Metro Manila in the Philippines. The study reviewed and included all the patient records from the years 2017-2019 which included skin biopsy results and slit-skin smear with AFS. Leprosy patients were then classified based on the following classifications: World Health Organization (WHO) and Ridley-Jopling classifications; and the concordance of clinical diagnosis vs the histopathologic findings and clinical diagnosis vs AFS results were determined using kappa testing.
الموضوعات
Leprosy , Biopsyالملخص
Ocular adnexal lymphoma is a common orbital malignant tumor.The incidence of ocular adnexal lymphoma is in-creasing rapidly each year,and the clinical manifestations and imaging characteristics of the disease are not specific.Therefore,the diagnosis of the disease relies on pathological tissue analysis.Accurate diagnosis of ocular adnexal lymphoma relies on mor-phology,immunohistochemistry,molecular and cytogenetics.With in-depth research on the disease,it has been found that the clinical prognosis of this tumor is characterized by high recurrence.This article mainly explains the correlation between high dis-ease recurrence rate and histopathological characteristics.
الملخص
Objective:To investigate the correlation between liver stiffness and histopathological changes in a rat model of acute hepatitis using virtual touch tissue imaging quantification (VTIQ) technology.Methods:A total of 100 SPF-grade SD rats were randomly divided into 3 groups: control ( n=30), low-dose ( n=35), and high-dose ( n=35) groups. Acute hepatitis models were induced in the low-dose and high-dose groups using 400 mg/kg and 600 mg/kg of Thioacetamide (TAA), respectively. Liver stiffness parameters of the right median lobe and right lobe were measured using VTIQ technology, Mean-H and Mean-L represent the liver lobes with higher and lower liver stiffness measurments, respectively, while Mean represent the average of the measurements from both liver lobes. Comparative analyses of liver stiffness parameters were performed across three groups and between the two lobes of the liver. The correlations between the Mean values of liver stiffness and semi-quantitative histopathological data were investigated. Ten rats were randomly selected from each of the 3 groups to test the repeatability of VTIQ values before and after euthanasia with intraperitoneal anesthesia. Subsequently, 10 rats after euthanasia from each 3 group were randomly chosen to assess the repeatability of VTIQ measurements for inter-observer and intra-observer variabilities. Results:VTIQ results showed statistically significant differences in Mean, Mean-H, and Mean-L among the 3 groups (all P<0.01). The high-dose group had higher measurements compared to the low-dose and control groups, with significant intergroup differences (all P<0.01). Significant differences in Mean-H and Mean-L were observed between the two liver lobes in both low and high-dose groups (all P<0.01). The Mean value showed significant positive correlations with semi-quantitative histopathological data of hepatocellular edema, periportal inflammatory cell infiltration, macrophage proliferation, and bile duct proliferation ( r=0.391, 0.648, 0.577, 0.542; all P<0.01). Multivariate linear regression analysis indicated that hepatocellular edema, eosinophilic change, and bile duct proliferation significantly and positively predicted the Mean value (β=-0.278, -0.196, -0.333; all P<0.05). There were no significant differences of VTIQ measurements befor and after euthanasia (all P>0.05), with repeatability coefficients of 0.166, 0.182, 0.185 for Mean, Mean-H, and Mean-L, respectively. Post-euthanasia, inter- and intra-observer VTIQ differences remained non-significant (all P>0.05), with Mean, Mean-H, Mean-L coefficients of 0.114, 0.194, 0.165 and 0.206, 0.322, 0.268, respectively. Conclusions:VTIQ technology demonstrates potential clinical value in assessing a rat model of acute hepatitis, offering a new perspective for non-invasive evaluation of acute hepatitis. However, its clinical application requires further validation.
الملخص
Polypoidal choroidal vasculopathy (PCV) is originally defined as a separate disease, but with the development of imaging techniques, it has now been included in the spectrum of neovascularization. In the Asian population, the prevalence of PCV is high, and with the deepening of clinical studies, the pathological characteristics, pathogenesis and clinical manifestations of PCV have been more deeply understood. Through dynamic observation and histopathological study of PCV lesions during operation, it can be confirmed that the lesions are mainly located between the retinal pigment epithelium and the Bruch membrane, rather than originating from the choroidal circulation, which is of great significance for understanding the origin and natural course of PCV. It is worth noting that although a theoretical bridge has been established between age-related macular degeneration (AMD)/PCV, there is a lack of intuitive clinical data on the ultrastructural and molecular manifestations of the cells/stroma in the local lesions of the eye, especially the progression of AMD/PCV from early/middle stage to exudative stage. It is precisely because of this that highly attractive research topics and exploration space are proposed for the future.
الملخص
@#Mass of the vulva include both benign and malignant lesions. Benign mass lesions of the vulva include tumors, hamartomas, cysts, infectious disorders, and non-neoplastic epithelial disorders. Some are rare mass lesions. Here, we presented three rare mass lesions of the vulva. The first case presented with the complaint of vulvar lesion since childhood, while the two cases presented in reproductive age and perimenopausal age. All three cases presented as mass lesions in the vulva with pain or uneasiness during work. In all these cases, excision was done. On histopathological examination, the lesions have different diagnoses which are common in other parts of the body, but rarely present at the vulva. A definitive diagnosis of a vulvar mass lesion is difficult to make, especially in the case of rare mass lesion. Histopathology is the principal tool of diagnosis.
الموضوعات
Lymphangioma , Fibroadenomaالملخص
The ICR(Institute of Cancer Research)mouse infection model was constructed to study the pathogenicity of Sal-monella Telelkebir serotype,and the pathogenic identification of mouse isolates was carried out.Observe the bacterial excretion cycle,evaluate the pathogenicity of Salmonella serotype to mice,and calculate the LD50 by the changes in clinical characteris-tics,histopathology and tissue bacterial load of infected mice;by flight mass spectrometry,biochemical identification,serotype identification,molecular typing and other experiments,compared with human isolates;virulence gene analysis was carried out by PCR experiment and whole genome sequencing.The LD50 of Salmonella Telelkebir is 2.67 × 108 CFU/mL;curling and fluffing may occur 0.5 h after infection;autopsy of dead mice showed that the small intestine was severely congested,with more bubbles and fluid accumulation,cecal necrosis,liver apical degeneration and necrosis,necrotic foci on the surface of the kidney and spleen atrophy;the bacterial load of spleen,kidney,lung,liver and jejunum in mice reached its peak at 3 days after infection,while that of heart at 6 days;the bacterial excretion time of the high-dose group exceeded 100 days;The level of CD3 in tissues increased with increasing dose,with inflammatory cell infiltration,myocardial capillary dilation and hyperemia,large area of vacuoles,degeneration and necrosis of hepatocytes,obvious enlargement of splenic sinus,blurred zoning,thickening of glomerular basement membrane,partial exfoliation of ciliated epithelium,atrophy and exfoliation of jejunal villi;PCR and whole genome sequencing revealed Salmonella-related virulence genes such as cdtB,plt A and pltB.This study was the first to successfully establish the ICR mouse model of Salmonella Telelkebir,demonstrating that this serotype of Salmonella has some pathogenicity.