الملخص
Abstract An adult male puma (Puma concolor), hit by a car in an urban area, died three days later despite the therapeutic support provided. At necropsy, multiple firm nodules were identified in the gastric mucosa. The nodules were coated by an intact mucosa with a central opening from which reddish and cylindrical nematodes protruded into the lumen. Twenty-seven nematodes were retrieved for morphological and morphometric evaluations. During histopathological examination of the gastric tissue, the adult nematodes appear in longitudinal and transverse sections, surrounded by thick bands of collagen, interspersed with mixed inflammatory infiltrates. The nematodes had an eosinophilic cuticle with caudal serrated projections (bulbar type), coelomyarian musculature, pseudocoelom, and females with uterus containing numerous larvated eggs, characteristics consistent with the Cylicospirura genus. Morphologically, female nematodes had six large tricuspid teeth in the oral cavity and the vulva had an opening anterior to the esophagus-intestinal junction. Male nematodes had five pairs of small papillae near the tip of the tail. These findings were consistent with Cylicospirura felineus. This parasite should be included in the differential diagnosis of nodular gastric wall lesions in wild felids.
Resumo Uma onça parda (Puma concolor) foi encontrada em uma área urbana após atropelamento e, apesar do suporte terapêutico fornecido, o animal morreu três dias depois. No exame post-mortem, múltiplos nódulos firmes foram identificados na mucosa gástrica. Os nódulos eram revestidos por mucosa intacta com um orifício central, do qual se insinuavam nematódeos cilíndricos e avermelhados. Vinte e sete nematódeos foram recuperados para avaliação morfológica e morfométrica. Na avaliação histopatológica do tecido gástrico, os nematódeos adultos apareceram em cortes longitudinais e transversais, circundados por bandas espessas de colágeno, intercaladas por infiltrado inflamatório misto. Os nematódeos eram constituídos por cutícula eosinofílica, com projeções serrilhadas voltadas caudalmente (do tipo bulbar), musculatura celomiariana, pseudoceloma e, nas fêmeas, útero com numerosos ovos larvados cujos achados foram sugestivos do gênero Cylicospirura. Morfologicamente, a cavidade bucal continha seis grandes dentes trífidos, na fêmea, a abertura da vulva era anterior à junção esôfago intestinal; e os machos tinham cinco pares de pequenas papilas próximas à ponta da cauda. Esses achados foram consistentes com Cylicospirura felineus. Este parasita deve ser incluído no diagnóstico diferencial de lesões nodulares da parede gástrica em felinos selvagens.
الموضوعات
Animals , Male , Female , Cats , Puma/parasitology , Gastritis/parasitology , Gastritis/veterinary , Nematoda/physiology , Cat Diseases/parasitology , Cat Diseases/pathology , Nematode Infections/parasitology , Nematode Infections/pathology , Nematode Infections/veterinaryالملخص
The tropical rattlesnake (Crotalus durissus) is a snake of great importance for biomedical industry since its poison is used for the production of antiophidic serum and researches. Several conditions related to animal health, such as diseases and parasites, which can promote the reduction of poison production by these snakes should be investigated. Accordingly, the aim of this study was to characterize the gross and microscopic lesions related to the presence of Ophidascaris arndti in stomachs of tropical rattlesnakes. The gastrointestinal tract of thirty-five South American rattlesnakes captured in Southeastern region of Brazil were analyzed and nineteen animals showed infestation by the parasites, found in the small intestine and, especially, in the stomach of the hosts. Grossly, lesions were characterized by mucosal ulcers occasionally associated with hemorrhage. Microscopic alterations included histiocytic granulomas, fibrosis, necrosis, and hemorrhage. Based on these findings, the diagnosis of a parasitic granulomatous disease was made. The lesions may be related to the cause of death in captivity snakes, since the lesions can promote secondary infections by opportunistic bacteria. Moreover, the intense inflammatory response accompanied by fibrosis may be related to poor functioning of the gastric snakes, which it may exhibit frequent regurgitation of the food.(AU)
A cascavel (Crotalus durissus) é uma serpente de grande importância para a indústria biomédica, uma vez que seu veneno é utilizado para a produção de soro antiofídico e para pesquisas. Condições diversas relacionadas ao estado de saúde do animal, como doenças e parasitoses, que possam promover a diminuição da produção de veneno por essas serpentes, devem ser investigadas. O objetivo deste estudo foi caracterizar os achados macro e microscópicos relacionados à presença de nematódeos Ophidascaris arndti nos estômagos de cascavéis. O trato gastrointestinal de 35 cascavéis capturadas na região Sudeste do Brasil foi avaliado Dessas, 19 apresentavam infestação pelo parasito, que foi encontrado predominantemente no estômago e no intestino delgado dos hospedeiros. Macroscopicamente, as lesões foram caracterizadas por úlceras nas camadas mucosas, ocasionalmente associadas com hemorragia. As alterações microscópicas apresentaram granulomas histiocíticos, fibrose, necrose e hemorragia. Baseado em tais achados, o diagnóstico de doença granulomatosa parasitária foi realizado. As lesões encontradas podem estar relacionadas à causa das mortes das serpentes em cativeiro, uma vez que podem favorecer infecções secundárias por bactérias oportunistas. Além disso, a intensa resposta inflamatória e a fibrose podem estar relacionadas ao mau funcionamento gástrico das serpentes, que podem apresentar frequente regurgitação do alimento.(AU)
الموضوعات
Animals , Crotalus , Gastritis/veterinary , Granuloma/veterinary , Nematoda , Fibrosis/veterinaryالملخص
Com o objetivo de determinar a epidemiologia e as características morfológicas, incluindo a localização anatômica, das lesões extrarrenais de uremia, bem como determinar as principais lesões do sistema urinário associadas à ocorrência de uremia, foram revisados os protocolos de necropsias de cães realizadas no Laboratório de Patologia Veterinária da Universidade Federal de Santa Maria de janeiro de 1996 a dezembro de 2012 (17 anos). Nesse período foram necropsiados 4.201 cães, sendo que 161 (3,8%) apresentaram lesões extrarrenais de uremia. Em 134 cães (83,2%) foram descritos sinais clínicos associados à uremia. As lesões extrarrenais mais frequentes, em ordem decrescente, foram: gastrite ulcerativa e hemorrágica (56,5%), mineralização de tecidos moles (55,9%), edema pulmonar (47,2%), estomatite e/ou glossite ulcerativa (30,4%), endocardite/trombose atrial e aórtica (28,6%), hiperplasia das paratireoides (9,3%), osteodistrofia fibrosa (8,1%), anemia (6,2%), laringite ulcerativa (5%), enterite ulcerativa/hemorrágica (3,7%), esofagite fibrinonecrótica (1,9%) e pericardite fibrinosa (1.9%). Na maioria dos casos as lesões extrarrenais de uremia foram decorrentes de azotemia prolongada por lesões renais graves, sendo as mais prevalentes a nefrite intersticial e a glomerulonefrite.
The aim of this study was to determine the epidemiology and the morphological characteristics (including the anatomic localization) of the extrarenal uremic lesions, as well as to describe the main lesions of the urinary system associated with the occurrence of uremia, through analysis of the protocols of necropsies performed in dogs from January 1996 to December 2012 (17 years) at the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. A total of 4,201 dogs were necropsied and 161 (3.8%) had extrarenal uremic lesions. In 134 dogs (83.2%) clinical signs associated with uremia were reported. The extrarenal lesions more often observed, in descending order of prevalence, were ulcerative and hemorrhagic gastritis (56.5%), soft-tissue mineralization (55.9%), pulmonary edema (47.2%), ulcerative stomatitis and/or glossitis (30.4%), endocarditis/atrial and aortic thrombosis (28.6%), parathyroid hyperplasia (9.3%), fibrous osteodystrophy (8.1%), anemia (6.2%), ulcerative laryngitis (5%), ulcerative and hemorrhagic enteritis (3.7%), fibrinonecrotic esophagitis (1.9%), and fibrinous pericarditis (1.9%). In most of the cases, the extrarenal lesions of uremia were due to prolonged azotemia secondary to severe renal lesions, such as interstitial nephritis and glomerulonephritis (the most prevalent ones).
الموضوعات
Animals , Dogs , Azotemia/veterinary , Urinary Tract/injuries , Uremia/epidemiology , Uremia/veterinary , Calcification, Physiologic , Pulmonary Edema/veterinary , Gastritis/veterinary , Gingivitis, Necrotizing Ulcerative/veterinaryالملخص
As doenças de chinchilas foram estudadas através da avaliação de laudos de necropsia entre janeiro de 1997 e dezembro de 2011. Em 202 chinchilas necropsiadas, 189 (93,5%) tiveram o diagnóstico determinado, e 13 (6,5%) tiveram diagnóstico inconclusivo, por ausência de lesões ou autólise acentuada. Dentre as 202 chinchilas computadas, 162 eram fêmeas (80%), 37 eram machos (18%), e em quatro chinchilas (2%) o sexo não foi anotado. As chinchilas tinham entre um dia a 12 anos de idade. As doenças foram agrupadas nas seguintes categorias: doenças inflamatórias, doenças causadas por intoxicações, doenças causadas por agentes físicos, doenças metabólicas, doenças parasitárias, doenças degenerativas, distúrbios circulatórios, neoplasmas, distúrbios do desenvolvimento e "outros distúrbios". As doenças inflamatórias foram as mais prevalentes (52 casos [25,7%]) e foram representadas por casos de gastrite (10 casos), listeriose (5 casos), septicemia (5 casos), broncopneumonia bacteriana (4 casos), enterite necrosante (4 casos), piometra (4 casos), diarreia com isolamento de Proteus sp. (3 casos), abscessos subcutâneos e em linfonodos (2 casos), endometrite (2 casos), otite (2 casos), pielonefrite (2 casos), abscesso do ligamento redondo do fígado (1 caso), pneumonia fibrinosa (1 caso), pneumonia intersticial (1 caso), hepatite e colecistite com isolamento de Salmonella sp. (1 caso), histiocitose pulmonar (1 caso), miosite linfo-histiocítica (1 caso) e um caso de dermatofitose (Trichopyton metagrophytes). O segundo grupo de doenças mais prevalentes foram as intoxicações (22,3%), representado por 45 casos de intoxicação por salinomicina. As doenças causadas por agentes físicos (21 casos [10,4%]) incluíam casos de traumas causados por outros animais (8 casos), automutilação após injeção intramuscular (8 casos), prolapso de reto (3 casos) e parto distócico (2 casos). A categoria de doenças metabólicas foi representada por 16 casos (7,9%) de lipidose hepática. As doenças parasitárias (8 casos [4%]) consistiram em infestação por pulga (4 casos), piolho (3 casos) e giardíase (1 caso). Doenças degenerativas (4 casos [2,5%]) incluíam insuficiência renal crônica (2 casos), necrose aleatória de hepatócitos (1 caso) e necrose muscular de origem desconhecida (1 caso). Os distúrbios circulatórios incluíram dois casos (0,99%) de insuficiência cardíaca congestiva. Neoplasmas foram representados por dois casos (0,99%) de adenocarcinoma gástrico. Um caso de atresia ani, associado a ausência do trato reprodutor, intestino grosso e rins policíticos representou a categoria de distúrbios do desenvolvimento (0,5%). Algumas doenças não se enquadraram nas categorias acima e foram enquadradas em "outros distúrbios" (38 casos [18,8%]). Nesta categoria, doenças dentárias foi o distúrbio mais comum, diagnosticado em 9% (18 de 202) de todas as chinchilas examinadas. Seguido por casos de hipertermia (14 casos), dois casos de anemia, dois casos de metaplasia de células adiposas do córtex da adrenal, e dois casos de mucometra.(AU)
Diseases of chinchilla were studied through the review of necropsy reports of 202 postmortem examinations carried out in this species from January 1997 to December 2011. One hundred and eighty nine of these necropsies (93.5%) had a conclusive diagnosis and in 13 (6.5%) a conclusive diagnosis was not reached due either absence of lesions or advanced autolysis. One hundred and sixty two (80%) of the necropsied chinchilla were females and 37 (18%) were males and the sex was not recorded in four cases (2%). Ages of necropsied chinchillas varied from one day to 12-years-old. The encountered diseases were grouped in the following categories: inflammatory diseases, diseases caused by intoxications, diseases caused by physical agents, metabolic diseases, parasitism, degenerative diseases, circulatory disturbances, developmental disorders and "other disorders". Inflammatory diseases were the most prevalent (52 cases [25.7%]) and included gastritis (10 cases), necrotizing enteritis (6 cases), listeriosis (5 cases), septicemia (5 cases), bacterial bronchopneumonia (4 cases), pyometra (4 cases), diarrhea associated with Proteus sp. (3 cases), subcutaneous and lymph node abscesses (2 cases), endometritis (2 cases), otitis (2 cases), pyelonephritis (2 cases), abscesses in hepatic the round ligament (1 cases), fibrinous pneumonia (1 case), interstitial pneumonia (1 case), hepatitis and cholecystitis associated with Salmonella sp. (1 case), pulmonary histiocytosis (1 case), and dermatophytosis by Trichopyton metagrophytes (1 case). The second most prevalent group of diseases was that caused by intoxications (22,3% of the cases) including 45 cases of intoxication by salinomycin. Diseases caused by physical agents (21 cases [10.4%]) included trauma cases caused by other animals (8 cases), self mutilation secondary to intramuscular injection (8 cases), rectal prolapsed (3 cases) and dystocia (2 cases). Metabolic diseases were represented by 16 cases (7.9%) of hepatic lipidosis. Parasitic diseases were represented by 8 cases (4%) flea (4 cases) lice (3 cases) infestations and one case o giardiasis. Degenerative diseases (4 cases or 2,5%) included two cases of chronic renal failure, one case of scattered hepatocellular necrosis and one case of muscle necrosis of unknown origin. Circulatory disturbances included two cases (0.99%) of congestive heart failure. Neoplasms were represented by two cases (0.99%) of gastric adenocarcinoma. Developmental disorders included one (0,5%) case of atresia ani associated with polycystic kidneys and absence of reproductive tract and large intestine. Thirty eight cases (18.8%) did not fit in any of the above categories and were placed as "other disorders". In this category dental disease was the most commonly (8 cases or 9%) diagnosed disorder, followed by 14 cases (6,9%) of hyperthermia, tow cases of anemia, two cases of fat metaplasia of adrenal cortex and two cases of mucometra.(AU)
الموضوعات
Animals , Tooth Diseases/veterinary , Chinchilla/immunology , Helicobacter Infections/veterinary , Gastritis/veterinary , Lipidoses/veterinary , Autopsy/veterinary , Cause of Death , Gastrointestinal Tract/physiopathologyالملخص
The aim of this study was to determine the omeprazol treatment effect on equine gastric fluid pH with food andwithout food administering for the duration of the experiment. To carry this project out, 7 animals were chosenbetween the ages of 3 and 10 (2 males and 5 females) from the Centro de Veterinaria y Zootecnia - CES. In thecontrolled treatment they received 200 ml of physiological saline and were treated using omeprazol pellets whichwere administered in a single dose of 4 mg/kg via a nasogastric insertion, and in two groups with or without foodduring the treatment. Gastric pHs were measured at 0 hour (fasting before treatment) and then at hours 3, 5, 7, 9,11 and 24 after treatment. In animals consuming food, both treatments showed post treatment gastric pH increases,which can be explained by the ad libitumn food management in both treatments, which may increasing post prandialpH. In fasting animals, we observed that in the controlled treatment the pH was maintained at very low levels atall measurement times, while in the omeprazol treatment the pH increased significantly. These results show thattreatment with omeprazol pellets at a dose of 4 mg/kg via nasogastric insertion increased gastric pH in healthyanimals that are not eating food and that this effect was similar to them being administered food.
El objetivo de este estudio fue determinar el efecto del tratamiento con omeprazol sobre el pH del fluido gástrico en equinos, con la administración del alimento y sin ella. Para llevar a cabo este proyecto se eligieron siete animalesentre los 3 y 10 años de edad (2 machos y 5 hembras) del Centro de Veterinaria y Zootecnia CES. El tratamientocon omeprazol se administró en pellets a una dosis única de 4 mg/kg, por vía nasogástrica, para los grupos cono sin alimento. Para el tratamiento control, se administraron 200 ml de solución salina fisiológica. En todos lostratamientos se midieron los pHs gástricos a la hora 0 (ayunas antes del tratamiento) y luego a las horas 3, 5, 7, 9, 11 y 24, postratamiento. Los animales que consumieron alimento con y sin omeprazol presentaron aumentos de pHgástrico postratamiento, lo que se puede explicar por el sumistro de heno ad libitum en ambos tratamientos. En losanimales en ayuno se observó que en el tratamiento control, el pH se mantuvo en niveles muy bajos en todas las horasde medición, mientras que en el tratamiento con omeprazol, el pH aumentó notablemente. Los resultados muestran que el tratamiento con pellets de omeprazol a una dosis de 4 mg/kg vía nasogástrica aumentó el pH gástrico en animales sanos que no están consumiendo alimento y que este efecto fue similar que cuando se administró el mismo.
A resistência é o mecanismo pelo qual a bactéria pode diminuir a ação dos agentes antimicrobianos de formanatural ou adquirida; dentro das causas mais frequentes está a utilização indiscriminada destes agentes em pacienteshumanos e produções pecuárias. Desde 1990 tem identificado novas cepas resistentes de bactérias que originaramdoenças zoonóticas emergentes. Dentro destas a zoonose, a salmonelose é considerada a doença mais difundida anível mundial, a ampla distribuição na natureza entre animais silvestres, domésticos e o meio ambiente, produz umarápida propagação de seus mais de 2500 sorotipos no homem. Contudo, a grande preocupação pela infecção com esteinteiro patógeno é devido aos cada vez mais elevados fracassos nos tratamentos com antimicrobianos convencionais,ocasionados pela alta resistência bacteriana a estes fármacos. O presente artigo é uma revisão sobre a problemáticaque gerou a resistência de Salmonella no homem e os animais, contem também estratégias de prevenção e controledeste fenômeno, que está distante de terminar e permitem o fortalecimento da saúde pública.
الموضوعات
Animals , Hydrogen-Ion Concentration , Food-Drug Interactions , Gastritis/veterinary , Omeprazole/therapeutic use , Animal Diseases/prevention & control , Animal Diseases/therapy , Food , Omeprazole/administration & dosage , Omeprazole/pharmacologyالملخص
The aim of this study was describe effects of NonsteroidalAnti-inflammatory drug on prevalence of Helicobacter LikeOrganisms in gastric mucosa of Thoroughbreds horses. Werestudied 54 Thoroughbred horses in the national race TrackLa Rinconada Caracas-Venezuela. All equine were treatedby seven days with phenylbutazone at an intravenous doseof 4.4 mg/kg. All horses presented Equine gastric ulcer syndromeacute superficial gastritis (25/54), chronic gastritis witherosion focal (16/54), chronic gastritis with erosion focal andulcers (14/54) in the gastric in both regions mucosa squamousregion and glandular regions (fundus). Helicobacter Like Organismsinfection in the stomach was confirmed by Warthin-Starry (38/54). Gastric mucosa revealed numerous spiralshapedbacteria morphologically resembling Helicobacter LikeOrganisms in squamous regions, margo plicatus (20/38) andnumerous spiral-shaped bacteria in fundic glands (18/54). Inconclusion, we detected high presence of Helicobacter LikeOrganisms in the gastric mucosa of Thoroughbred horsestreatment with phenylbutazone.
الموضوعات
Animals , Anti-Inflammatory Agents , Horse Diseases/pathology , Gastritis/diagnosis , Gastritis/therapy , Gastritis/veterinary , Helicobacter Infections/pathology , Helicobacter Infections/veterinary , Horses/injuriesالملخص
Non-H. pylori helicobacters (NHPH) have been demonstrated as gastric spiral-shaped bacteria in specimens obtained from dogs; however, their roles in the pathogenesis of upper gastrointestinal disease have not yet been clearly established. The purpose of this study was to evaluate the prevalence of NHPH DNA in the gastric mucosa of dogs and its association with histopathology. Helicobacter was detected through histopathological techniques, PCR, and FISH analysis from fundic biopsies of twenty dogs with or without signs of gastrointestinal disease. PCR and FISH were based on partial 16S rRNA gene sequences. Nineteen dogs showed mild to marked gastritis in the fundus, and only one dog had a healthy gastric mucosa. NHPH DNA was detected in 18 dogs with gastritis and one with normal gastric mucosa. However, there was no significant correlation between the presence of NHPH DNA and the degree of gastritis. These results show a high prevalence of NHPH DNA in the gastric mucosa of dogs from Venezuela. Further studies are necessary to determine a possible association between a specific NHPH species and the degree of gastritis.
Los helicobacteres no-H. pylori (NHPH, por sus siglas en inglés) han sido demostrados como bacterias gástricas de forma espiral; sin embargo, sus roles en la patogénesis de la enfermedad gastrointestinal superior no han sido claramente establecidos. El propósito de este estudio fue evaluar la prevalencia de ADN de los NHPH en la mucosa gástrica de perros y su asociación con histopatología. Helicobacter fue detectado a través de técnicas histopatológicas, análisis de PCR y FISH en biopsias del fundus gástrico de 20 perros con o sin signos de enfermedad gastrointestinal. La PCR y FISH se basaron en secuencias parciales del gen ARNr 16S. Diecinueve perros mostraron gastritis leve a marcada en el fundus gástrico y sólo un perro tuvo una mucosa gástrica sana. El ADN de los NHPH fue detectado en 18 perros con gastritis y uno con mucosa gástrica normal. Sin embargo, no hubo correlación significativa entre la presencia de ADN de los NHPH y el grado de gastritis. Estos resultados demuestran una alta prevalencia de ADN de los NHPH en la mucosa gástrica de perros de Venezuela. Futuros estudios son necesarios para determinar la posible asociación entre una especie específica de los HNPH y el grado de gastritis.
الموضوعات
Animals , Dogs , Female , Male , Dog Diseases/microbiology , Gastric Mucosa/microbiology , Gastritis/veterinary , Helicobacter Infections/veterinary , Helicobacter/genetics , DNA, Bacterial/analysis , Gastric Mucosa/pathology , Gastritis/microbiology , Gastritis/pathology , Helicobacter Infections/microbiology , Helicobacter/isolation & purification , In Situ Hybridization, Fluorescence , Phylogeny , Polymerase Chain Reaction/veterinary , /genetics , Venezuelaالملخص
Con el objeto de determinar la frecuencia de presentación de Helicobacter spp. se realizó un estudio en caninos de Valdivia, Chile, a través de biopsia gástrica de las zonas fúndica y pilórica obtenida por endoscopía y analizadas mediante las pruebas de ureasa e histología. Los resultados indican que la bacteria Helicobacter spp. fue detectada en todos los perros. La prueba de ureasa dio resultados positivos a Helicobacter en el 86,2 por ciento de las muestras de la zona fúndica y en el 75,9 por ciento del antro pilórico. El examen histológico reveló la bacteria en todas las muestras fúndicas y en el 92 por ciento de las muestras del antro pilórico. En la zona fúndica las frecuencias obtenidas entre la prueba de ureasa e histología resultaron estadísticamente significativas, a diferencia de lo que se obtuvo en la zona del antro pilórico. La bacteria fue encontrada en perros de todas las edades de ambos sexos. Las muestras que resultaron positivas a la prueba de ureasa, reaccionaron principalmente entre las 3 y 24 horas de incubación. En el examen histológico se determinó el grado de infección por el Helicobacter spp., siendo las muestras clasificadas mayormente en los grados moderado a marcado, ubicándose sobre la superfiscie de la mucosa, en las fosas gástricas, glándulas gástricas y en las células parietales.
A study was carried out in order to determinate Helicobacter spp. in dogs of Valdivia city, Chile. Gastric mucosal biopsy from fundus and pyloric antrum were obtained by endoscopy and analized by urease test and histology.Stomach endoscopic appearance, frecuency presentation of Helicobacter spp. according to sex and age, time reaction to urease test, degree of infection and distribution Helicobacter spp. were analized. Results indicated that Helicobacter spp. was detected in all dogs. The urease test was positive in 86.2% of the fundus samples and in 75.9% of the pyloric antrum samples. Histological examination revealed the bacterium in all of the fundus samples and in 92% of the antrum samples. In fundus samples the frecuency obtained by urease test and histology resulted statistically significant, different to the results obtained in the antrum region. Helicobacter spp. was found in young and old animals of both sexes. Samples that gave positive results in the urease test reacted mainly between 3 to 24 hours post incubated. Histological examination to determinate degree of infection to Helicobacter spp., were classified moderate to marked degree and detected on the mucosal surface, gastric pits, gastric glands and parietal cells.
الموضوعات
Animals , Dogs , Endoscopy, Gastrointestinal/methods , Endoscopy, Gastrointestinal/veterinary , Gastritis/diagnosis , Gastritis/veterinary , Helicobacter/pathogenicity , Urease/analysis , Biopsy/methods , Histological Techniques/methodsالملخص
O objetivo deste estudo foi determinar a ocorrência de lesões gástricas em suínos de abate e verificar a relação das lesões com o gênero, o peso da carcaça e a presença de Helicobacter spp na mucosa gástrica. Foram colhidos e examinados 236 estômagos de suínos. As lesões da pars esophagea e região glandular foram classificadas conforme a severidade em graus 0, 1, 2 e 3. Fragmentos das regiões aglandular e glandular foram processados para exame histológico e para pesquisa de Helicobacter spp. A análise macroscópia reveleu que 203 (86.1) estômagos apresentavam algum tipo de lesão. A ocorrência de erosões e úlceras na região aglandular foi observada em 104 (44.1) animais e na região glandular em 22 (9.3). A ulceração na região gastroesofágica estava presente em 45 animais (19.1), dos quais 25 (21.1) eram machos e 20 (16.5) fêmeas. O peso médio da carcaça foi de 82.7 kg nos animais sem lesões e com paraqueratose na pars esophagea e de 79.5 Kg nos animais com erosões e ulcerações. Utilizando a coloração de Warthin-Starry, observamos Helicobacter spp na mucosa gástrica de 112 (47.5) amostras. Destas, 54 (48.2) foram classificadas como grau 2 ou 3 (pars esophagea) e 58 (51.8) como grau 0 e 1. Dos animais positivos para o helicobacter spp, 26 (23.2) apresentavam úlceras na pars esophagea e 24 (21.4) apresentavam a mucosa sem alterações. Dos 124 (52.5) animais negativos, 50 (40.3) foram classificados como grau 2 ou 3 e 74 (59.7) como grau 0 ou 1. A análise estatística revelou que não há diferença significativa entre suínos com ou sem lesões gástricas em relação á presença de helicobacter spp, gênero e peso da carcaça
The aim of this study was to investigate the association between the lesions of the gastric mucosa inslaughter pigs and sex, carcass weight and presence of Helicobacter spp. Stomachs from 236 swinewere examined. Gastroesophageal lesions were classified in agreement severity in grade 0, 1, 2 and 3.Fragments from the aglandular and glandular area were processed for histology and for identification ofHelicobacter spp in tissue sections. Macroscopic analysis revealed 203 (86.1%) stomachs with somedegree of lesion. Grade 2 and 3 lesions were observed in 104 (44.1%) animals. Grade 3 lesions wereobserved in 25 (21.1%) castrated males and 20 (16.5%) females. The average carcass weight of animalswith grade 0 and 1 lesions and grade 2 and 3 was 82,5 Kg and 79,5 Kg, respectively. One hundred andtwelve (47.5%) pigs were positive for Helicobacter spp. by Warthin-Starry stain method ; among them,54 (48.2%) had grade 2 and 3 lesions; and 58 (51.8%) had grade 0 and 1 lesions. One hundred and twenty-four (52.5%) were negative for Helicobacter spp; among them 50 (40.3%) had grade 2 and 3 lesions, and74 (59.7%) had grade 0 and 1. There was no significant difference between pigs with and without gastriclesions in regard to the presence of Helicobacter spp, sex and weight