ABSTRACT
Recombinant LC3-encoded protein is highly antigenic, immunogenic and protective in gerbil model of amebic liver abscess as subunit vaccine with titermax as an adjuvant. The aim of this work is to study the LC-3 incoded protein as a subunit vaccine. To reach our goal two groups of BALB/c mice had immunized with LC3-encoded protein and cholera toxin [CT] as an adjuvant. Group I immunized with l.0Mg of LC3 with 10 ug of cholera toxin group II with 10 ug of cholera toxin only. We found that secretory IgA, serum IgA and serum IgG were siginificantly increased in group I compared with group II [p<0.0001]. In conclusion, oral immunization of BALB/c mice with recombinant LC3-encoded lectin with CT as an adjuvant was effective in inducing intestinal and systemic IgA and IgG