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Objective:To detect the expression of ARK5 in peripheral blood circulating tumor cells (CTCs) from pancreatic cancer patients and explore its correlation with the efficacy and prognosis for gemcitabine chemotherapy.Methods:A total of 175 peripheral blood samples of pancreatic cancer patients who were treated in the Department of Hepatobiliary Surgery, Second Affiliated Hospital of Jiaxing University from January 2016 to June 2021 were collected. CTCs were enriched by nano-microfluidic chip technology. The expression of ARK5 in CTCs was detected by immunofluorescence. According to the expression of ARK5, the patients were divided into two groups: positive group and negative group. The differences on clinicopathological features, the efficacy of chemotherapy, median survival and progression-free survival time between the two groups were compared.Results:CTCs were enriched in 98 of 175 patients (55.6%), including 70 ARK5 positive and 28 ARK5 negative patients. There were no significant differences on clinical features between the two groups, and the two groups were comparable. In the 70 ARK5 positive patients, 64 patients (91.4%) were resistant to gemcitabine, while only 12 of the 28 ARK5 negative patients (42.8%) were resistant to gemcitabine. The incidence of gemcitabine resistance in ARK5 positive patients was significantly higher than that in ARK5 negative patients, and the difference was statistically significant ( P<0.001). The median survival time was 11.5 months in ARK5 positive group and 14 months in ARK5 negative expression group, and the progression-free survival time was 6 months in ARK5 positive expression group and 8 months in negative expression group. The survival time of ARK5 positive group was significantly shorter than that of ARK5 negative group, and the difference was statistically significant ( P<0.05). Conclusions:The pancreatic cancer patients with ARK5 positive CTCs have significantly higher incidence of gemcitabine resistance and shorter survival time than those with ARK5 negative CTCs. Detection of ARK5 expression in CTCs may be a new method to judge chemotherapy efficacy and prognosis for pancreatic cancer patients.
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Objective @#To explore the effects of different implant sites combined with pterygomaxillary implantation on the biomechanics of implant and bone tissue，and to provide a basis for clinical selection of implant design in accordance with biomechanical principles．@*Methods@#The cone-beam CT scan data of an edentulous maxillary with inadequate posterior bone were selected to complete the establishment of the three-dimensional entity model of the maxilla．The prosthesis and implant-abutment integrated three-dimensional entity models were established using the prosthesis and implant data ，and five groups of three-dimensional finite element models of different implant sites were designed．A unilateral vertical load of 200 N and an oblique load of 100 N were applied to the bilateral posterior dental area，respectively．ANSYS finite element analysis software was used to calculate the stress distribution on the surface of the implant and surrounding bone tissue．SPSS 26. 0 software package was used to statistically analyze the data. @*Results @#① All the five models showed that the maximum stress was concentrated in the neck of the implants and cortical bone. ② The maximum stress value of the implant under oblique loading was greater than that under vertical loading (P＜0. 05) ，but there was no significant difference in the maximum stress value around bone tissue in the 5 groups of models. ③ There was no significant difference in the maximum stress of the implant and bone between the different implant sites combined with pterygomaxillary implants．@*Conclusion @#In the fixed restoration of maxillary edentulous implants，pterygomaxillary implants implanted symmetrically on both sides and changing the position point of the anterior implant do not affected the stress distribution of the whole design．In clinical practice，suitable sites can be selected according to the residual bone mass of patients，and combined with pterygomaxillary implantation for implant design.
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OBJECTIVE@#To investigate the expression of Calponin-1 and Transgenlin in the uterine smooth muscles during normal labor on-sets, and to evaluate their effect on initiating the normal labor.@*METHODS@#A total of 14 uterine bodies and lower segments of human pregnancy were divided to a non-labor group (NIL) and a labor group(IL). Immunohistochemical technology and Western blot were used to determine the expression of Calponin-1 and Transgelin in the 2 groups.@*RESULTS@#Immunohistochemical detection and Western blot showed that Calponin-1 protein in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues had significant difference (P0.05).@*CONCLUSION@#Calponin-1 of the uterine smooth muscle and Transgelin of the uterine body smooth muscle may involve in the regulation of uterine smooth muscle contractility, which is closely related to child birth launch.
Subject(s)
Adult , Female , Humans , Pregnancy , Calcium-Binding Proteins , Genetics , Metabolism , Labor Onset , Metabolism , Microfilament Proteins , Genetics , Metabolism , Muscle Proteins , Genetics , Metabolism , Myometrium , Metabolism , Uterine Contraction , MetabolismABSTRACT
Objective To investigate the effect of Calponin-1 suppression on human myometrium cells through adenovirus mediated siRNA. Methods Human uterine smooth muscle tissues were digested with enzymes, cultured and confirmed with immunocytochemistry. Aadenovirus siRNA-Calponin-1 plasmid was transfected into primary cultured uterine smooth muscle cells in vitro. The expressions of Calponin-1 mRNA and protein were analyzed by RT-PCR and Western blot, respectively.Results The pAdEasy-pShuttle-U6-Calponin-1 siRNA plasmid was successfully constructed, and Calponin-1 siRNA mediated by recombinant adenovirus resulted in markedly reduced expression of Calponin-1 mRNA and protein in human myometrium cells. The gray values of Calponin-1 mRNA in the uterine smooth muscle cells in the experimental, blank control, and empty vector groups were 316.3±39.2, 1048.5±126.4 and 1027.2±127.5, respectively. The gray values of Calponin-1 protein were 323.3±43.2, 1021.5±143.4, and 1019.2±144.5,respectively. The difference between the experimental group and the blank control group as well as the empty vector group was significant (P< 0.05). There was no significant difference between the empty vector group and the blank control group (P>0.05).Conclusion The pAdEasy-pShuttle-U6-Calponin-1 siRNA plasmid can inhibit the expression of Calponin-1 in human myometrium cells in vitro,which may be a useful approach to determine the role of Calponin-1 in delivery.
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Objective To explore the effects of passive smoking on pregnant rats and embryo development.Methods The pregnant Wistar rats were exposed to passive smoking4times per day,10min per time,continuously for20days,and the body weights were measured at the1st day,10th day and20th day.At the at20th day,the concentrations of NO in serum of pregnant rats were determined,simultansly the fetal death,embrgonic absorption were observed,the body weights,body length,tail length,brain weight and live weight of fetuses fetal growth retardation(IUGR)were observed.Results Significantly higher frequencies of fetal death,embryonic absorption,IUGR and significantly lower concentration s of NO in serum of pregnant rats were found in experiment group exposed to passive smoking.Significantly lower levels of body weight,body length,tail length,brain weight of fetuses were found in experiment group exposed to passive smoking compared with those of controls(P