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1.
Tropical Biomedicine ; : 86-93, 2021.
Article in English | WPRIM | ID: wpr-886076

ABSTRACT

@#The study was aimed to evaluate the effectiveness of maggot therapy in healing of cutaneous infected wound in streptozotocin (STZ) induced diabetic Wistar rat. For live maggots, the sterilized eggs of Lucilia sericata were obtained from colonies established in laboratory. Diabetes model was established in 48 male Wister rat by intra-peritoneal injection of STZ at the dose of 60 mg/kg body-weight. Cutaneous wounds exposed with mixed colonies of bacteria like Staphylococcus aureus, E. coli and Pseudomonas aeruginosa were prepared in all rat. The animals equally divided in 4 groups with 12 rats each being presented as treatment group of control, antibiotic, maggot and maggot with antibiotic in combination. All treatments were done once and hold for 24 hours. Wound kinetics and bacterial bio burden were measured at weekly interval to till complete healing. Significant reduction in wound area with maximum contraction was found (>95%) in maggot treated group when compared to antibiotic treated (79%) and control (72%). In maggot as well as maggot and antibiotic in combination group showed early elimination of bacterial bio-burden 7.88±0.03log CFU/ml to 1.12±0.65log CFU/ml and 7.86±0.04) log CFU/ml to 1.54±0.52log CFU/ml respectively in three weeks of time. Early healing indication was also experienced on histomorphological examination of wounded tissue of maggot treated groups by early and better epithelialization, collagenation and neovascularization with complete healing of wound in three weeks in comparison to antibiotic and control respectively. However, the present study did not show any difference in healing of wound with use of maggot alone or in antibiotic combination. Live maggot of Lucilia sericata effectively lower bacterial bioburden and and accelerate healing of infected cutaneous wound in diabetic conditions.

2.
Indian J Ophthalmol ; 2020 Mar; 68(3): 516-517
Article | IMSEAR | ID: sea-197843
4.
Tropical Biomedicine ; : 627-638, 2018.
Article in English | WPRIM | ID: wpr-750431

ABSTRACT

@#Delayed wound healing due to extraneous bacterial contamination, antibacterial resistance and other associated factors are of great concern in dealing patients having chronically infected wound. Medicinal properties of certain maggots of Calliphoridae family are known for its effective wound debridement therapy. The objective of the study was to evaluate the wound healing potential of maggots of Lucilia sericata in an experimentally infected cutaneous wound model in Wistar rat. The study was carried out by using male Wistar rats (n=48) by creating excisional wounds and later contaminated with mixed population of gram positive and gram-negative bacteria. Animals were divided randomly in to four groups with 12 individuals each, being denominated as control, antibiotic treated, maggot treated, and antibiotic plus maggot combination treated group. Ten pre-sterilized maggots were applied per centimetre square wound bed for 24 hours. Different wound kinetics in L. sericata maggot treated wounds revealed significant reduction in wound area with maximum contraction, early elimination of bacterial bioburden as compared to group of infected control and group of rats receiving only antibiotic treatment. The histopathological examination of wounded tissue of maggot treated groups showed early and better epithelialization, collagenation and neovascularization with complete healing of wound in two weeks. The maggot effects on healing when used singly or in combination with antibiotic were recorded to be similar. The results of the present study clearly demonstrate that the maggots of L. sericata possesses a definite antibacterial action along with removal of dead tissues and effectively reduced the bacterial bio-burden in infected wound and induced wound healing quickly.

5.
Indian J Exp Biol ; 2007 Aug; 45(8): 744-8
Article in English | IMSEAR | ID: sea-59880

ABSTRACT

Phenolic extract of leaves of Basilicum polystachyon (L) Moench was tested for in vitro antimicrobial activity against five bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Micrococcus leuteus) and three fungi (Fusarium oxysporum, Aspergillus niger, Helminthosporium oryzae). Efficacy of organic solvents, methanol and ethanol, as agents for extraction was compared with acidic water (2M; HCl). High-pressure liquid chromatographic (HPLC) data showed that acidic extraction (2M; HCl) resulted in higher yield of caffeic acid (0.437 mg g(-1)) and rosmarinic acid (0.919 mg g(-1)). Acidic extract showed high activity against Gram (+) ve bacteria, but was less active against Gram (-) ve bacteria. Amongst the tested fungi, maximum activity was exhibited against Aspergillus niger. This is the first report on the phenolic constituents and bioactivity of B. polystachyon.


Subject(s)
Anti-Infective Agents/isolation & purification , Bacteria/drug effects , Caffeic Acids/isolation & purification , Chromatography, High Pressure Liquid , Cinnamates/isolation & purification , Depsides/isolation & purification , Fungi/drug effects , Lamiaceae/chemistry , Microbial Sensitivity Tests , Plant Extracts/chemistry , Plant Leaves/chemistry , Solvents/chemistry
8.
J Indian Med Assoc ; 2000 Jul; 98(7): 394-5
Article in English | IMSEAR | ID: sea-105217

ABSTRACT

Schwannomas are slow growing benign tumours arising from the nerve sheath. Two cases of schwannoma arising from the vagus nerve are presented here. One case was found in a 6-year-old boy and another one in a 30-year-old man. In the first case the tumour was detected on the left side of the neck just below the angle of the mandible, and in the second case it was found on the lower part of the right side of the neck. Histopathology confirmed the diagnosis as first one to be of Antoni-A type and the second one to be of Antoni-B type. Both the tumours were excised and on follow-up the patients were doing well.


Subject(s)
Adult , Biopsy, Needle , Child , Cysts/diagnosis , Diagnosis, Differential , Follow-Up Studies , Head and Neck Neoplasms/diagnosis , Humans , Male , Neurilemmoma/diagnosis , Treatment Outcome
9.
Article in English | IMSEAR | ID: sea-111992

ABSTRACT

Sixty strains of Escherichia coli, were isolated and identified from 94 samples of infantile diarrhoea and traveller's diarrhoea. Eleven strains were found to be colicinogenic and colicin 'H' was found to be predominant in conjunction with other colicins. Five strains were enteropathogenic of which 055 and one untypable strain were highly enteropathogenic. A positive correlation could be established between haemolytic character, dulcitol fermentation and the virulence of the strain involved.


Subject(s)
Adult , Colicins/biosynthesis , Escherichia coli/classification , Escherichia coli Infections/microbiology , Gastroenteritis/microbiology , Humans , Infant , Infant, Newborn , Virulence
10.
Hindustan Antibiot Bull ; 1995 Feb-Nov; 37(1-4): 37-43
Article in English | IMSEAR | ID: sea-2266

ABSTRACT

A streptomycete strain D1.5 capable of producing broad spectrum antiobiotic was isolated from soil. The morphological, cultural, physiological and biochemical characters were studied, compared to known species and identified as Streptmoyces hygroscopicus. Antibiotic activity of the strain was tested against both Gram positive and negative bacteria as well as fungi. It exhibited complete resistance to beta-lactum antibiotics.


Subject(s)
Anti-Bacterial Agents/biosynthesis , Culture Media , Drug Resistance, Microbial , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Lactams , Microscopy, Electron, Scanning , Soil Microbiology , Spores/ultrastructure , Streptomyces/growth & development
11.
Indian J Physiol Pharmacol ; 1993 Jul; 37(3): 241-3
Article in English | IMSEAR | ID: sea-107414

ABSTRACT

Effect of 75 mg/kg of body weight of cimetidine administered intraperitoneally daily for 14 days to two groups of experimental animals (one of the experimental group was having intact testis and another group was bilaterally orchidectomized) was observed on cell population & cell volume of gonadotrophs and lactotrophs in pituitary gland, as it has not been studied earlier. In the experimental group of intact testis, there was significant reduction in the cell population of FSH cells in the cephalomedian area (P < 0.001) and in the lateral lobe (P < 0.01); the volume of both FSH and LH cells was also significantly reduced. In group 4 and group 5 there was significant increase in the population of lactotrophs and also in the volume of LH cells, FSH cells & lactotrophs. The change in the gonadotrophs in group 2 was due to increased production of testosterone from hypertrophied Leydig cells of testis rather then it's direct effect on adenohypophysis; in group 4 and group 5 the changes were due to lack of testosterone as in those cases bilaterally orchidectomy already done.


Subject(s)
Animals , Cimetidine/pharmacology , Follicle Stimulating Hormone/biosynthesis , Leydig Cells/drug effects , Luteinizing Hormone/biosynthesis , Male , Mice , Orchiectomy , Organ Size/drug effects , Pituitary Gland, Anterior/cytology
12.
Article in English | IMSEAR | ID: sea-16840

ABSTRACT

The effects of phenobarbitone on gonadotrophs and lactotrophs of pituitary gland were studied in male albino mice which were administered phenobarbitone 50 mg/kg body weight intraperitoneally (ip) daily for 14 days. A group of control mice were administered saline ip. In the experimental group, there was significant reduction in the average cell population of FSH cells in the cephalomedian area (P < 0.001) and in the lateral lobe (P < 0.01). The average volume of both FSH and LH cells was also significantly reduced (P < 0.001). The changes in the gonadotrophs of the experimental group could be attributed to the negative feedback action of testosterone which is being secreted from hypertrophied Leydig cells of testis.


Subject(s)
Animals , Follicle Stimulating Hormone/metabolism , Luteinizing Hormone/metabolism , Male , Mice , Phenobarbital/pharmacology , Pituitary Gland/drug effects
13.
Indian J Pediatr ; 1993 Mar-Apr; 60(2): 257-63
Article in English | IMSEAR | ID: sea-81698

ABSTRACT

Diazepam used in the treatment of eclampsia crosses the placental barrier readily, and may cause various clinical effects in the neonates. Twenty-five (25) live born babies of eclamptic mothers receiving diazepam were studied and cord blood diazepam concentration was estimated. Effect of low dose of diazepam is minimal apart from lowering of rectal temperature and the effects lasted for a period of 12 hours. But high dose (> 30 mg) of diazepam and prolonged duration of diazepam therapy in mothers causes significant depression of the newborn and the effects lasted for a period of 36-48 hours. As the clinical condition of the newborn is not related to the diazepam concentration in cord blood, the cord blood estimation is not helpful in the assessment of clinical effects of the drug in newborn. The tissue storage of the drug in newborn appears to be responsible for the clinical effects.


Subject(s)
Adult , Apgar Score , Body Temperature/drug effects , Diazepam/administration & dosage , Dose-Response Relationship, Drug , Eclampsia/metabolism , Female , Fetal Blood/chemistry , Humans , Infant, Newborn , Maternal-Fetal Exchange , Pregnancy , Prospective Studies
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