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1.
Chinese Critical Care Medicine ; (12): 494-496, 2021.
Article in Chinese | WPRIM | ID: wpr-883914

ABSTRACT

Oral feeding of water cannot be accomplished in some critical patients due to coma, mouth-breathing or long-term indwelling of gastric tube, causing dry mouth which results in oral pain, ulcer, or infection, aggravating patients' discomfort and even affecting the prognosis. To solve this problem, the medical staff of the First Hospital of Jiaxing developed a novel oral humidifier and obtained the National Utility Model Patent of China (ZL 2019 2 0066979.0). The oral humidifier consists of a spraying device and a fixation device. The spraying device is a spraying housing with a water tank, and a spraying port where nanometer water mist can be emitted. The spraying housing is also equipped with a flashlight with a switch, and a schematic diagram of pupil size, which can be used to assess the pupil size conveniently. The fixing device is composed of a fixing frame, an adjustable rod and a clamp. The two ends of the adjustable rod are connected to the fixing frame and the clamp respectively, and can be adjusted to proper shape. The spraying housing can be placed on the fixing frame and installed on the bed and adjusted to the proper position quickly. This novel oral humidifier is a simple device which combines a spraying system with a pupil evaluation tool, implying significant clinical application in improving the convenience of nursing and alleviating dry mouth of critical patients.

2.
Article in Chinese | WPRIM | ID: wpr-872129

ABSTRACT

Objective:To observe the clinical effect of the improved eyebrow lifting operation through the incision under the eyebrow to improve the upper eyelid skin relaxation and explore its application scope.Methods:From March to October 2019, 32 female patients in the outpatient department of Huangsi Plastic Surgery Hospital underwent the improved eyebrow lifting operation through the incision under the eyebrow.Results:After the improved eyebrow lifting procedure, the blepharoptosis of the upper eyelid in 32 patients was significantly improved, and there was no operative complication, and the eyebrow shape and eyebrow arch fullness were satisfactory.Conclusions:The eyebrow arch is full, and the eyebrow shape and the distance between eyebrow and eye are not changed significantly after the operation.

3.
Chinese Critical Care Medicine ; (12): 1125-1127, 2020.
Article in Chinese | WPRIM | ID: wpr-866979

ABSTRACT

Objective:To explore the application of folding transfer shelf in the transportation of critically ill patients.Methods:Patients transferred from the emergency department to the intensive care unit (ICU) admitted to the First Hospital of Jiaxing from January 1st to December 31st in 2019 were enrolled. The patients were divided into study group and control group by whether or not using the self-developed folding transfer shelf. The incidence of adverse events, the stability rate of vital signs and the transport time were compared between the two group.Results:A total of 437 patients were enrolled in the study, with 222 in the study group (which used the self-developed folding transfer shelf) and 215 in the control group (which used the conventional stretcher). The baseline data such as gender, age, disease status and disease severity were balanced between the two groups. The stability rate of vital signs in the study group was higher than that in the control group (89.19% vs. 82.33%, P < 0.05). The transfer time in the study group was shorter than that in the control group (minutes: 6.39±1.35 vs. 7.61±1.34, P < 0.01). The total incidence of adverse transport events in the study group was lower than that in the control group (2.25% vs. 10.23%, P < 0.01). The incidence of miscarriage of emergent materials and instrument falling in the study group were lower than those in the control group (0% vs. 2.79%, 0% vs. 2.33%, both P < 0.05). Conclusions:The folding transfer shelf could reduce the transport risk of critical ill patients, especially the risk of miscarriage and falling of rescue instrument. The application of folding transfer shelf could regulate the management of transport, keep the vital signs of patients stable during transport, shorten the transport time, and facilitate an efficient and high-quality transport.

4.
Chinese Critical Care Medicine ; (12): 110-111, 2020.
Article in Chinese | WPRIM | ID: wpr-866759

ABSTRACT

Multifunctional monitors including electrocardiograph monitor, oxygen saturation monitor, blood pressure monitor, temperature monitor, arterial pressure monitor are often used for critically ill patients in hospitals. Each function monitoring part is equipped with multiple data monitoring wires. Due to the disorderly distribution, monitoring wires are often intertwined with each other. The medical staff will spend much time for careful identification and energy in emergency use and the improper operation of the equipment leads to its damage, which could affect data monitoring result, delay the diagnosis and treatment, and cause certain medical risks. In addition, the data line is often exposed to the external environment, which has a certain impact on its service life. Moreover, the distribution of the data line is disorderly, affecting the hospital environment and appearance. Thus, a kind of medical wire storage box was designed, which could realize the monitoring data cables in proper storage and neat distribution, reducing the workload of medical staff. The wire storage box has won the National Utility Model Patent of China (ZL 2017 2 1826195.0). The introduction goes as following. The storage box includes upper box body, lower box body and winding roller. The receptacle on the upper box body and the lower box body cooperate to form a cylindrical receptacle chamber for the winding roller. The side wall of the winding roller is designed with spiral winding groove which could help wrap the excessively long wires in the winding groove for storage. When multiple boxes need to be used simultaneously, the strip and slot on both sides of the lower box are used to splice the cable boxes together which improves the uniformity with less space use. The medical storage box is easy to make and can effectively avoid line friction and damage, prolong the line service life, and save department cost. In addition, it avoids the involvement between the lines, and improves the work efficiency. It also helps to keep the department environment tidy, which complies with "6S" management requirements, with good clinical application value.

5.
Article in Chinese | WPRIM | ID: wpr-864846

ABSTRACT

Objective:To understand the reasons for non-adherence of pulmonary rehabilitation exercise in patients with acute exacerbation of COPD, and in order to provide theoretical and practical basis for improving patient adherence.Methods:Using qualitative phenomenological research methods, eighteen patients of non-adherence with exercise were conducted deeply semi-structured interviews, and the Colaizzi analysis was used for data analysis and extracting themes.Results:Seven themes were extracted: effect of subjective and objective symptoms, limited by comorbidity, negative mood disturbance, lack of perception effect, material environment in ward, cultural environment in ward and inappropriate form of exercise.Conclusions:Health care providers should value these obstacles and implement targeted measures, in order to achieve the goal of improving exercise adherence, so that more patients would complete pulmonary rehabilitation exercise.

6.
Article in Chinese | WPRIM | ID: wpr-879505

ABSTRACT

OBJECTIVE@#To explore the clinical and genetic characteristics of a child featuring developmental delay.@*METHODS@#The child was subjected to whole exome sequencing. Candidate variant was verified by Sanger sequencing.@*RESULTS@#Whole genome sequencing revealed that the child has carried compound heterozygous variants c.2607-1G>C and c.899 + 2dupT of the RAB3GAP1 gene, which were respectively derived from her mother and father.@*CONCLUSION@#A rare case of Warburg micro syndrome type 1 was diagnosed. The phenotype of the child was consistent with the literature, in addition with dysplasia of palatine arch, prominent high palatal arch and tooth dysplasia. Above finding has provided a basis for genetic counseling and prenatal diagnosis for the family.


Subject(s)
Abnormalities, Multiple/genetics , Adult , Cataract/genetics , Child , Cornea/abnormalities , Female , Humans , Hypogonadism/genetics , Intellectual Disability/genetics , Male , Microcephaly/genetics , Mutation , Optic Atrophy/genetics , Whole Exome Sequencing , rab3 GTP-Binding Proteins/genetics
7.
Chinese Journal of Pathology ; (12): 127-131, 2019.
Article in Chinese | WPRIM | ID: wpr-810451

ABSTRACT

Objective@#To observe the clinicopathologic features of oropharyngeal squamous cell carcinoma associated with human papilloma virus (OPSCC-HPV) and discuss the role and value of different in situ hybridization (ISH) detection methods for HPV in pathologic diagnosis.@*Methods@#Fifteen cases of OPSCC-HPV were collected from Department of Pathology, Beijing Tongren Hospital, Capital Medical University from January 2016 to August 2018. These cases were diagnosed in accordance with the WHO classification of head and neck tumors. The histopathologic features and the clinicopathologic data were retrospectively analyzed. Immunohistochemistry (two-step EnVision method) was done to evaluate the expression of p16, Ki-67 and p53. ISH was used to detect HPV DNA (6/11 and 16/18). RNAscope technology was used to evaluate the presence of HPV mRNAs (16 and 18).@*Results@#The mean age for the 15 patients (8 males, 7 females) was 47 years (range from 30 to 69 years). OPSCC-HPV typically presentedat an advanced clinical stage, six patients had cervical lymphadenopathy (large and cystic), seven had tonsillar swelling, one had tumor at base of tongue, and one had odynophagia. Microscopically the tumors exhibited distinctive non-keratinizing squamous cell carcinoma morphology. Cervical nodal metastases were large and cystic, with thickening of lymph node capsules. OPSCC-HPV raised from crypt epithelium and extended beneath the tonsillar surface epithelial lining as nests and lobules, often with central necrosis. Tumor cells displayed a high N: C ratio, and high mitotic and apoptotic rates. Tumor nests are often embedded within lymphoid stroma, and may be infiltrated by lymphoid cells.Fifteen cases (15/15) were strongly positive for p16; Ki-67 index were 60%-90%; they were focally positive or negative for p53. Ten cases (10/10) were negative for HPV 6/11 DNA, and one case(1/10) was focally positive for HPV16/18 DNA. Eleven cases (11/11) were strongly positive for HPV16 mRNA, one case was focally positive for HPV18 mRNA.@*Conclusions@#OPSCC-HPV is a pathologically and clinically distinct form of head and neck squamous cell carcinoma. OPSCC-HPV is associated with high-risk HPV (type 16) in all cases. Detection of high-risk HPV16 mRNA by RNAscope is of great significance in the final diagnosis and pathogen identification.

8.
Article in Chinese | WPRIM | ID: wpr-773471

ABSTRACT

OBJECTIVE@#To construct a recombinant lentiviral expression vector pCDH-Daxx-EGFP to investigate the effect of Daxx on the proliferation of vascular smooth muscle cells (VSMCs).@*METHODS@#The recombinant lentiviral expression vector pCDHDaxx-EGFP was constructed using PCR-based accurate synthesis method. After identification by sequencing and enzyme digestion, the recombinant lentiviral vector was contransfected into 293T cells with lentivirus packaging vector. The recombinant lentivirus particles were collected and purified to infect VSMCs, whose expression of Daxx was detected with Western boltting. The cells infected with the empty vector pCDH-EGFP or pCDH-Daxx-EGFP were incubated in serum-free medium or in the presence of angiotensin Ⅱ (AngⅡ). The cell viability was determined with MTT assay, and the cell cycle changes were analyzed with flow cytometry. The cell migration ability was assessed using a scratch wound healing assay. The expression of p-Akt protein in the cells was detected using Western blotting.@*RESULTS@#Double enzyme digestion and sequencing confirmed successful construction of the recombinant plasmid. Compared with the cells infected with the empty vector, the cells infected with pCDH-Daxx-EGFP exhibited significantly increased expressions of Daxx protein ( < 0.05). AngⅡ treatment of the cells infected with the pCDH-Daxx-EGFP, as compared with the cells infected with the empty vector, significantly lowered the cell viability, S phase cell ratio and cell migration ability ( < 0.05), and significantly decreased the expression level of p-Akt protein ( < 0.05).@*CONCLUSIONS@#We successfully constructed the recombinant lentiviral vector pCDH-Daxx-EGFP and overexpressed Daxx in primary cultured VSMCs using this vector. Daxx overexpression can inhibit AngⅡ-induced proliferation and migration in VSMCs probably by regulating p-Akt protein.

9.
Chinese Journal of Stomatology ; (12): 725-729, 2018.
Article in Chinese | WPRIM | ID: wpr-807594

ABSTRACT

Objective@#To investigate and compare the sensitivities of early childhood caries detection by using international caries detection and assessment system (ICDAS)-Ⅱ and WHO criteria.@*Methods@#A total of 449 3-year-old children from four day care kindergartens in Beijing were enrolled in this study. Both ICDAS-Ⅱ and WHO criteria were used to assess the prevalence of caries in the given subjects. The decayed, missing, and filled teeth (dmft) index scores were calculated. In ICDAS-Ⅱ system, four cut-off points (D1, D2, D3 and D4) were employed to differentiate sound and decayed teeth: D1 (score 0 as sound, scores 1-6 as caries); D2 (0-1 sound, 2-6 caries); D3 (0-2 sound, 3-6 caries) and D4 (0-3 sound, 4-6 caries). SPSS software was used to analyze the data to decide the significance of differences.@*Results@#The caries prevalence using ICDAS-Ⅱ were 76.6% (344/449), 71.3% (320/449), 52.8% (237/449) and 46.1% (207/449) for D1 to D4, respectively; the corresponding mean dmft scores were 4.95±4.85, 4.41±4.77, 2.54±3.69 and 1.97±3.10. The sites with highest caries prevalence were occlusal surface of mandibular molars in groups of D1 and D2 and proximal surface of maxillary anterior teeth in groups D3 and D4. In contrast, the caries prevalence was 48.8% (219/449) and the mean dmft was 2.27±3.54 when using WHO criteria, significantly lower than the detection rates by using ICDAS-Ⅱ (D1-D2) (P=0.00). This suggested that ICDAS-Ⅱ system was a more sensitive method in detecting early childhood caries.@*Conclusions@#ICDAS-Ⅱ system might be superior in detection of incipient caries and be of specific value in prevention of early childhood caries.

10.
Article in Chinese | WPRIM | ID: wpr-711546

ABSTRACT

Objective To investigate the safety and efficacy of endoscopic submucosal dissection ( ESD) for early stage colorectal cancer and precancerous lesions. Methods Clinical data of 108 patients who received ESD for early stage colorectal cancer and precancerous lesions from December 2016 to June 2017 in Renmin Hospital of Wuhan University were analyzed. The lesion characteristics, postoperative pathological features, intraoperative and postoperative complications and postoperative follow-up outcomes were analyzed. Results The 108 patients all underwent ESD successfully with median operation time of 45 min. The rate of intraoperative perforation and postoperative delayed bleeding was 2. 8% ( 3/108) and 2. 8% (3/108), respectively. No postoperative delayed perforation occurred. Postoperative pathology showed that there were 41 cases ( 38. 0%) of tubular adenoma, 4 ( 3. 7%) villous adenoma, 39 ( 36. 1%) villous tubular adenoma [ including 41 ( 38. 0%) low-grade intraepithelial neoplasia and 16 ( 14. 8%) high-grade intraepithelial neoplasia] , 19 ( 17. 6%) adenocarcinoma, and 5 ( 4. 6%) other types. Among the 19 cases of adenocarcinoma, there were 11 cases of well-differentiated, 5 median-differentiated and 3 low-differentiated. The complete resection rate was 100. 0% and the en bloc resection rate was 92. 3% ( 100/108) . The mean follow-up time was 8. 1 months, and no recurrence was found during this period. Conclusion ESD is safe and effective in the treatment of early stage colorectal lesions. It is important to improve preoperative assessment, strengthen surgical skills, analyze postoperative pathological features and regularly follow up to guarantee the treatment quality of ESD.

11.
The Journal of Practical Medicine ; (24): 3216-3219, 2017.
Article in Chinese | WPRIM | ID: wpr-658467

ABSTRACT

Objective To analyze the diagnostic value of dynamic digital radiography of hysterotubography combined with hydrotubation in utero-tubal factors in infertility. Methods Totally 480 female patients with infertil-ity caused by utero-tubal factors from June 2015 to March 2017 were divided into group A and group B randomly. After the two groups were all examined by dynamic digital radiography of hysterotubography ,group A received man-ual push and group B hydrotubation. Results and safety of radiography of two groups were analyzed. Results Im-age definition of group B was higher than that of group A. Number of dots and adverse reaction were less in group B than those in group A(P<0.05),but procedural time was longer than that of group A(P<0.05). The difference in the diagnostic rates of the disease of two groups was not statistically significant(P>0.05). Conclusion Dynamic digital radiography of hysterotubography combined with hydrotubation has good diagnostic value in infertility caused by utero-tubal factors and less adverse reactions

12.
The Journal of Practical Medicine ; (24): 3216-3219, 2017.
Article in Chinese | WPRIM | ID: wpr-661386

ABSTRACT

Objective To analyze the diagnostic value of dynamic digital radiography of hysterotubography combined with hydrotubation in utero-tubal factors in infertility. Methods Totally 480 female patients with infertil-ity caused by utero-tubal factors from June 2015 to March 2017 were divided into group A and group B randomly. After the two groups were all examined by dynamic digital radiography of hysterotubography ,group A received man-ual push and group B hydrotubation. Results and safety of radiography of two groups were analyzed. Results Im-age definition of group B was higher than that of group A. Number of dots and adverse reaction were less in group B than those in group A(P<0.05),but procedural time was longer than that of group A(P<0.05). The difference in the diagnostic rates of the disease of two groups was not statistically significant(P>0.05). Conclusion Dynamic digital radiography of hysterotubography combined with hydrotubation has good diagnostic value in infertility caused by utero-tubal factors and less adverse reactions

13.
Article in Chinese | WPRIM | ID: wpr-512035

ABSTRACT

Objective To preliminarily explore the feasibility of tree shrew as a new kind of animal model in research of amblyopia,to discuss the primary visual cortex plasticity mechanism of form deprivation in tree shrew,and to provide a theoretical basis for further understanding the mechanism of amblyopia formation and recovery.Methods Sixty 30-days old tree shrews were divided into five groups,12 in each group:the group A had the right eye sutured for 1 month;the group B had the right eye sutured for 2 months;the group C had the left eye sutured for 1 month and then opened and the righ eye was sutured for 1 month,in other words,the group C was performed by alternating suture;the tree shrews of control group 1(D1) were in the same age as the the group A,but fed in normal breedingenvironment;the tree shrews of control group 2(D2) were at the same age of groups B and C,but fed with a normal diet.Samples of the visual cortex were taken after the completion of modeling,and were processed to observe the histology and ultrastructure of the visual cortex,the neuron apoptosis,and the c-fos protein expression in the tree shrews of different groups.Results Damages to different degrees were found by histological and electron microscopic examination of the visual cortex in each experimental group,and they were more obvious in the group sutured for 2 months.TUNEL staining showed that there were no significant differences between the apoptosis in the experimental and control groups.The expression of c-fos mRNA and protein in the experimental groups was decreased,and it was the lowest in the group sutured for 2 months.There was a small increase in the c-fos expression after the alternate suture,and no significant difference of c-fos expression was found in the control groups.Conclusions Different degrees of deprivation amblyopia lead to different histopathological changes.There is a plasticity in the neurons affected by amblyopia.Tree shrew can be used as an ideal animal model for the studies of form deprivation amblyopia.

14.
Chinese Circulation Journal ; (12): 54-57, 2017.
Article in Chinese | WPRIM | ID: wpr-508137

ABSTRACT

Objective: To observe the impact of cardiac resynchronization therapy (CRT) on ventricular remodeling in patients with III°atrio-ventricular block (AVB) combining systolic dysfunction. Methods: A total of 49 III °AVB patients received CRT in our hospital from 2009-01 to 2014-10 were studied. Echocardiography was conducted at pre-operation and 6, 12 months post-operation to measure left ventricular ejection fraction (LVEF), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic volume (LVEDV), left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD) and mitral regurgitation (MR) grade in order to observe the changes of cardiac structure and function in relevant patients. Results: Compared with pre-operative condition, at 6 months post-operation, LVEF was increased (4.92±5.24)%and at 12 months post-operation, it was further increased (5.02±6.52)%, both P Conclusion:CRT may reverse ventricular remodeling and improve cardiac function in patients with III°AVB combining systolic dysfunction.

15.
Article in Chinese | WPRIM | ID: wpr-614799

ABSTRACT

BACKGROUND:Volar locking plate is the domin Pedicle screw placement for spinal fractures with X-ray and CT assistant can optimize the accuracy of screw placement.Additionally,X-ray is cheap,repetitive and can reduce radiation injury,but has not been confirmed.OBJECTIVE:To evaluate the effect and imaging evaluation of X-ray in pedicle screw placement for spinal fractures.METHODS:Twenty patients with spinal fractures at T1-8 levels in our hospital from November 2014 to December 2016 were enrolled.The needles were placed along the pedicle axis at the middle of the pedicle,vertebral posterior and vertebral anterior,and its position was recorded by C-arm X-ray,100 pedicle screws were placed under C-arm X-ray,and then evaluated by CT to analyze the application effect of X-ray.RESULTS AND CONCLUSION:The screw length between the actual placement at T1,T2,T3,T4,T5,T6,T7,T8 screw length under C-arm X-ray and virtual ones had no significant differences (P > 0.05),suggesting that X-ray can optimize the accuracy of screw placement.A total of 100 screws were placed,94 screws were excellent,which were placed into the vertebral body completely,and the direction and depth were in accordance with the requirements.Six screws were good,some screws slightly worn out,but the cortical bone was less,and none was poor.No accident of pedicle violations was found.To conclude,C-arm X-ray-assisted screw placement for spinal fractures exhibits ideal outcomes.

16.
Chinese Circulation Journal ; (12): 766-770, 2017.
Article in Chinese | WPRIM | ID: wpr-614228

ABSTRACT

Objective: To analyze the predictors of left ventricular reverse remodeling in patients with III? atrio-ventricular block (AVB) combining left ventricular systolic dysfunction after cardiac re-synchronization therapy (CRT). Methods: A total of 65 III? AVB patients received CRT in our hospital from 2009-01 to 2015-05 were enrolled. Clinical information before and after the operation were recorded. Left ventricular reverse remodeling was deifned by left ventricular end systolic volume (LVESV) decreased 15% or left ventricular ejection fraction (LVEF) increased≥5% at 12 months after CRT. The patients were divided into 2 groups: Reversal group,n=36 and No reversal group,n=29. Clinical condition was compared between 2 groups, predictors for CRT reversing left ventricular remodeling were evaluated by two classiifcation Logistic regression analysis. Results: The patients' average age was (62±14) years and 36/65 (55.4%) with reverse remodeling. In Reversal group, the ratios of female (P=0.011), baseline QRS width>120ms (P=0.001), inter-ventricular mechanical delay (IVMD)≥40 ms (P=0.027) and standard deviation of time-to-minimum systolic volume of 16 segments [Tmsv16-SD (%R-R)≥8.3%, (P=0.001)] were higher than those in No reversal group. Two classiifcation Logisitic regression analysis indicated that female (OR=6.228, 95%CI 1.561-24.842, P=0.01), QRS duration>120 ms (OR=7.778, 95% CI 1.996-30.769,P=0.003) and Tmsv16-SD (%R-R)≥8.3% (OR=8.134, 95% CI 2.064-32.057,P=0.003) were the independent predictors for ventricular reverse remodeling . Conclusion: Female, QRS>120ms and Tmsv16-SD (%R-R)≥8.3% could be used as the predictors for CRT reversing left ventricular remodeling in III? AVB patients combining left ventricular systolic dysfunction.

17.
Chinese Circulation Journal ; (12): 151-155, 2016.
Article in Chinese | WPRIM | ID: wpr-487000

ABSTRACT

Objective: To explore the inlfuence of diastolic iflling pattern on cardiac resynchronization therapy (CRT) in patients with ischemic cardiomyopathy. Methods: A total of 61 patients with ischemic cardiomyopathy received CRT in our hospital from 2012-03 to 2014-03 were studied. According to pre-CRT diastolic iflling pattern, the patients were divided into 2 groups:Non-restrictive iflling (NRF) group, n=36 and RF group, n=25. All patients were followed-up for 12 months, based on NYHA classiifcation, CRT efifcacy was assessed by echocardiography;the endpoints included re-hospitalization for heart failure or cardiac death. Kaplan-Meier survival curve was used to assess the prognosis. Results: ①NRF group had CRT response rate at 66.7%(24/36) which was higher than RF group 28.0%(7/25), (χ2=8.826, P=0.003);the post-operative NYHA classiifcation, LVEF, FS, LVEDV and LVESV were signiifcantly improved, all P Conclusion: Diastolic iflling pattern affected CRT efifcacy in patients with ischemic cardiomyopathy;NRF patients were more beneifciary for CRT, while RF patients had lower response to CRT which associated to poor prognosis.

18.
Chinese Pharmacological Bulletin ; (12): 1526-1530, 2016.
Article in Chinese | WPRIM | ID: wpr-501625

ABSTRACT

Aim To explore the anti-proliferation effects of curcumin trinicotinate ( CurTn ) on vascular smooth muscle cell ( VSMC ) and its mechanism. Methods The cells were cultured in DMEM supple-mented with 10% fetal bovine serum. MTT assay was used to examine cell proliferation. FCM was used to observe cell cycle. The expressions of PCNA, Cy-clinD1 and p-ERK1/2 were analyzed using Western blot. Results CurTn could inhibit the proliferation of VSMC and showed a certain amount-time relationship. What’ s more, CurTn could increase the G1 phase pro-portion of cell, decrease the S phase proportion and the expression level of PCNA protein. It was also found that CurTn significantly inhibit the protein expression of p-ERK1/2 and Cyclin D1 . Conclusion CurTn may inhibit the proliferation of VSMC via downregulating the expression of CyclinD1 and p-ERK1/2 .

19.
Article in Chinese | WPRIM | ID: wpr-494104

ABSTRACT

ObjectiveTo study the effects of 9 different drying methods on total saponin content and antioxidant capacity ofParis polyphylla var.yunnanensis.Methods UV-VIS spectrophotometry was used for measuring the total saponin content ofP. polyphylla var.yunnanensis, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiozoline-6)-sulphonic acid (ABTS+) radical scavenging methods were used for evaluating the antioxidant capacity ofP. polyphylla var. yunnanensisextracts.ResultsThe total saponin contents ofP. polyphylla var.yunnanensis dried by different methods existed significant difference. The sample dried at 35℃ showed highest total saponin content (17.557 mg/g). The sample dried in the shade naturally showed middle total saponin content (13.740 mg/g). Based on the results of antioxidant capacity detected by two methods, the samples ofP. polyphylla var. yunnanensis dried by 9 different methods all showed good antioxidant capacity and showed a certain concentration dependence.Conclusion The samples dried at 35℃ have the highest antioxidant capacity. Therefore, drying at 35℃ is a better drying method to obtain high quality and high activity ofP. polyphylla var.yunnanensis.

20.
Chinese Journal of Pathology ; (12): 900-904, 2015.
Article in Chinese | WPRIM | ID: wpr-278504

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the clinical and pathologic features of middle ear adenoma (MEA).</p><p><b>METHODS</b>Eight cases of MEA were collected from Beijing Tongren Hospital, Capital Medical University between 2004 and 2014, and immunohistochemical staining was performed.</p><p><b>RESULTS</b>The patients included five women and three men (mean age, 37.5 years; median 37 years; range, 21-51 years). All patients had unilateral lesions. Five MEA occurred on the left side, and three on the right. In seven patients the MEA was primary, and they presented with hearing loss (6 cases), tinnitus (5 cases), sense of ear blockage (3 cases), otalgia (1 case) and facial nerve paralysis (1 case). The remaining patient had recurrent MEA, and presented with otorrhea, aural fullness and tinnitus. Histologically, the tumor cells were arranged in a variety of patterns, including solid sheets, nests, glands, ribbons or trabeculae. Glandular structures were prominent in one case only. Immunohistochemically, the tumor cells were diffusely positive for keratin (8/8) and vimentin (8/8), and focally positive for CK 7(8/8) and CK5/6(8/8). CK7 and CK5/6 were predominantly positive in tumor cells with glandular growth pattern; CK7 was positive in the luminal cells while CK5/6 was positive in the abluminal cells. Both were also expressed focally in scattered tumor cells with non-glandular pattern. The tumor cells were also diffusely positive for synaptophysin(8/8), diffusely but weakly positive for NSE (5/8), and were diffusely or focally positive for chromogranin A (4/8). Both S-100 protein and calponin were negative in all cases. The proliferation rate was low, about 1%-2%. Six cases were followed up for one year and three months to ten years and six months, with an average follow-up period of four years and two months. Two patients developed recurrence, but there were no regional or distant metastases.</p><p><b>CONCLUSIONS</b>Diagnosis of MEA requires pathologic confirmation since the clinical symptoms are non-specific. MEA can show a variety of histologic patterns, and should be distinguished from other space-occupying lesions at this site. Immunohistochemical staining has greatly contributed to the diagnosis and differential diagnosis of MEA. The prognosis of this tumor is good. Patients with MEA require long-term follow-up for recurrences.</p>


Subject(s)
Adenoma , Pathology , Adult , Beijing , Diagnosis, Differential , Ear Neoplasms , Pathology , Ear, Middle , Pathology , Female , Humans , Male , Middle Aged , Neoplasm Recurrence, Local , Young Adult
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