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Tropical Biomedicine ; : 86-93, 2021.
Article in English | WPRIM | ID: wpr-886076


@#The study was aimed to evaluate the effectiveness of maggot therapy in healing of cutaneous infected wound in streptozotocin (STZ) induced diabetic Wistar rat. For live maggots, the sterilized eggs of Lucilia sericata were obtained from colonies established in laboratory. Diabetes model was established in 48 male Wister rat by intra-peritoneal injection of STZ at the dose of 60 mg/kg body-weight. Cutaneous wounds exposed with mixed colonies of bacteria like Staphylococcus aureus, E. coli and Pseudomonas aeruginosa were prepared in all rat. The animals equally divided in 4 groups with 12 rats each being presented as treatment group of control, antibiotic, maggot and maggot with antibiotic in combination. All treatments were done once and hold for 24 hours. Wound kinetics and bacterial bio burden were measured at weekly interval to till complete healing. Significant reduction in wound area with maximum contraction was found (>95%) in maggot treated group when compared to antibiotic treated (79%) and control (72%). In maggot as well as maggot and antibiotic in combination group showed early elimination of bacterial bio-burden 7.88±0.03log CFU/ml to 1.12±0.65log CFU/ml and 7.86±0.04) log CFU/ml to 1.54±0.52log CFU/ml respectively in three weeks of time. Early healing indication was also experienced on histomorphological examination of wounded tissue of maggot treated groups by early and better epithelialization, collagenation and neovascularization with complete healing of wound in three weeks in comparison to antibiotic and control respectively. However, the present study did not show any difference in healing of wound with use of maggot alone or in antibiotic combination. Live maggot of Lucilia sericata effectively lower bacterial bioburden and and accelerate healing of infected cutaneous wound in diabetic conditions.

Tropical Biomedicine ; : 627-638, 2018.
Article in English | WPRIM | ID: wpr-750431


@#Delayed wound healing due to extraneous bacterial contamination, antibacterial resistance and other associated factors are of great concern in dealing patients having chronically infected wound. Medicinal properties of certain maggots of Calliphoridae family are known for its effective wound debridement therapy. The objective of the study was to evaluate the wound healing potential of maggots of Lucilia sericata in an experimentally infected cutaneous wound model in Wistar rat. The study was carried out by using male Wistar rats (n=48) by creating excisional wounds and later contaminated with mixed population of gram positive and gram-negative bacteria. Animals were divided randomly in to four groups with 12 individuals each, being denominated as control, antibiotic treated, maggot treated, and antibiotic plus maggot combination treated group. Ten pre-sterilized maggots were applied per centimetre square wound bed for 24 hours. Different wound kinetics in L. sericata maggot treated wounds revealed significant reduction in wound area with maximum contraction, early elimination of bacterial bioburden as compared to group of infected control and group of rats receiving only antibiotic treatment. The histopathological examination of wounded tissue of maggot treated groups showed early and better epithelialization, collagenation and neovascularization with complete healing of wound in two weeks. The maggot effects on healing when used singly or in combination with antibiotic were recorded to be similar. The results of the present study clearly demonstrate that the maggots of L. sericata possesses a definite antibacterial action along with removal of dead tissues and effectively reduced the bacterial bio-burden in infected wound and induced wound healing quickly.

J Indian Med Assoc ; 2007 Jan; 105(1): 53-5
Article in English | IMSEAR | ID: sea-99051


A case of a 45-year-old lady presenting with symptoms suggestive of pancreatitis is described. Initially the findings on Imaging were ambiguous while the enzyme studies were against the diagnosis of pancreatitis. Recurrence of symptoms and with Increased intensity prompted repeated imaging and further investigations. Finally, by a combination of CT scan, MR imaging and muscle biopsy the rare diagnosis of pyomyositis was established. The fact that this case was difficult to diagnose because of its rarity and its mimicking pencreatitis is discussed.

Abdominal Abscess/diagnosis , Abdominal Muscles/microbiology , Biopsy , Diagnosis, Differential , Female , Humans , Magnetic Resonance Imaging , Middle Aged , Pyomyositis/diagnosis , Tomography, X-Ray Computed
J Indian Med Assoc ; 2003 Nov; 101(11): 672-4
Article in English | IMSEAR | ID: sea-96316


A young lady initially found to have hydatid cysts in the lung only, subsequently within a very short period was found to develop cysts in the liver. Soon after, she developed cysts in the subcutaneous tissue over the anterolateral chest wall, which, on investigation, revealed hepatic cysts herniating through the chest wall defects caused by previous operations. The unique features of this case include the degree of dissemination, the multiplicity of sites and the peculiar nature of herniation of the hepatic cysts into the parietes.

Adult , Echinococcosis, Hepatic/diagnosis , Echinococcosis, Pulmonary/diagnosis , Female , Humans
Indian J Exp Biol ; 2002 Jan; 40(1): 109-10
Article in English | IMSEAR | ID: sea-56609


A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.

Animals , Bacterial Toxins/genetics , Blotting, Southern , Cloning, Molecular , Clostridium perfringens/classification , DNA Primers/chemistry , DNA, Bacterial/analysis , Genes, Bacterial , Molecular Sequence Data , Polymerase Chain Reaction , Restriction Mapping , Sequence Analysis, DNA
J Indian Med Assoc ; 1971 Dec; 57(12): 464
Article in English | IMSEAR | ID: sea-96680