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1.
Chinese Journal of Nephrology ; (12): 739-748, 2021.
Article in Chinese | WPRIM | ID: wpr-911897

ABSTRACT

Objective:To explore the effect and involved mechanism of naringenin on acute kidney injury (AKI) induced by ischemia-reperfusion (IR).Methods:The IR-AKI rat model was constructed using the classic bilateral renal pedicle clamping method, then renal function and pathological change were assessed, as well as inflammation-associated genes were detected by quantitative real-time PCR. The hub genes were selected through differential gene analysis and protein-protein interaction network analysis, and their transcription factors were predicted, which constructed a protein library together. The proteins binding to naringenin were selected by reverse molecular docking analysis and further their binding patterns were predicted to explore the mechanism of naringenin. Finally, the results of bioinformatics were verified by experimental methods.Results:Compared with the AKI group, the kidney pathology of the rats in the naringenin pretreatment group was significantly improved, and the renal tubular injury score was reduced ( P<0.01); meanwhile the serum creatinine level and the mRNA expression of the kidney injury molecule 1 (KIM-1) were significantly decreased (both P<0.05). Compared to sham group, IR-AKI increased the level of nuclear factor κB (NF-κB), tumor necrosis factor-α and interleukin-1β (all P<0.05), which reversed by naringenin indicated that naringenin inhibited inflammation in IR-AKI. Differential gene analysis was performed on the GSE98622 data set, and 359 differential genes were obtained. In reverse molecular docking, the proteins with smallest binding energy including NFKBIA, BCL3, NFKB2 and RELA were considered to be related to the preventive effect of naringenin, which were mainly enriched in NF-κB-related inflammation pathways. Domain functional analysis of NF-κB-related genes showed that naringenin could stably bind to its key domain. According to quantitative real-time PCR results, naringenin increased BCL3 level after AKI ( P<0.05), and further decreased the expression level of RELA and NFKB2 (both P<0.05). Conclusion:Naringenin protects IR-AKI by alleviating inflammation, and its mechanism is related to increasing BCL3 and thereby inhibiting the NF-κB pathway.

2.
Asian Journal of Andrology ; (6): 80-84, 2021.
Article in English | WPRIM | ID: wpr-879731

ABSTRACT

This study investigated the correlation between periprostatic fat thickness (PPFT) measured on magnetic resonance imaging and lower urinary tract symptoms, erectile function, and benign prostatic hyperplasia (BPH) progression. A total of 286 treatment-naive men diagnosed with BPH in our department between March 2017 and February 2019 were included. Patients were divided into two groups according to the median value of PPFT: high (PPFT >4.35 mm) PPFT group and low (PPFT <4.35 mm) PPFT group. After the initial evaluation, all patients received a combination drug treatment of tamsulosin and finasteride for 12 months. Of the 286 enrolled patients, 244 completed the drug treatment course. Patients with high PPFT had larger prostate volume (PV; P = 0.013), higher International Prostate Symptom Score (IPSS; P = 0.008), and lower five-item version of the International Index of Erectile Function (IIEF-5) score (P = 0.002) than those with low PPFT. Both high and low PPFT groups showed significant improvements in PV, maximum flow rate, IPSS, and quality of life score and a decrease of IIEF-5 score after the combination drug treatment. The decrease of IIEF-5 score was more obvious in the high PPFT group than that in the low PPFT group. In addition, more patients in the high PPFT group underwent prostate surgery than those in the low PPFT group. Moreover, Pearson's correlation coefficient analysis indicated that PPFT was positively correlated with age, PV, and IPSS and negatively correlated with IIEF-5 score; however, body mass index was only negatively correlated with IIEF-5 score.

3.
Article in Chinese | WPRIM | ID: wpr-875672

ABSTRACT

Objective To establish the chemical synthesis of the active ingredient rosavin of Rhodiola rosea. Methods β-D-pentaacetylglucose, 1-hydroxy-2,3,4-triacetylarabinose and cinnamyl alcohol were used as starting materials. The target compound was prepared by 1-position selective of β-D-pentaacetylglucose deacetylation, glycosylation reaction, glucose 6-OH selective protection and deprotection and other 8-step reactions. Results The target product, rosavage, was successfully obtained with high yield. The structure was confirmed by ESI-MS, 1H-NMR and 13C-NMR. The protection of 6-OH with high selectivity and high yield of tert-butyldiphenyl chlorosilane played a vital role in the synthesis process,. Conclusion The synthetic route has the advantages of simple operation, high yield, and good safety.

4.
Article in Chinese | WPRIM | ID: wpr-862478

ABSTRACT

Signal transducer and activator of transcription 3 (STAT3) is a signal transcription protein that exists in the cytoplasm. The abnormal activation of STAT3 is closely related to cell proliferation, differentiation, and canceration. It has abnormal expression in cancer stem cells such as breast cancer, pancreatic cancer, lymphoma, and lung cancer. Therefore, inhibiting the abnormal expression of STAT3 has become a new approach for antitumor therapy.

5.
Article in Chinese | WPRIM | ID: wpr-826667

ABSTRACT

OBJECTIVE@#To compare the clinical therapeutic effect of long-snake moxibustion and ginger-partitioned moxibustion at point on nonspecific low back pain (NLBP) with symptom of cold and dampness.@*METHODS@#A total of 120 patients were randomized into a long-snake moxibustion group, an ashi point group and a waiting for treatment group, 40 cases in each one. Ginger-partitioned moxibustion was applied from Dazhui (GV 14) to Yaoshu (GV 2) of governor vessel in the long-snake moxibustion group, and was applied at point of affected area in the ashi point group, 40 min each time, once every other day and totally 8 times were required. No intervention was adopted in the waiting for treatment group, and after the trial, long-snake moxibustion was applied. Before and after treatment, the visual analogue scale (VAS) scores of rest and activity, the Oswestry disability index (ODI) score and the score of cold and dampness symptom were observed in the 3 groups.@*RESULTS@#Compared before treatment, the VAS scores of rest and activity, the ODI scores and the scores of cold and dampness symptom after treatment were decreased in the long-snake moxibustion group and the ashi point group (<0.05). After treatment, the variations of the above indexes in the long-snake moxibustion group and the ashi point group were larger than those in the waiting for treatment group (<0.05), and the variations of the above indexes in the long-snake moxibustion group were larger than those in the ashi point group (<0.05).@*CONCLUSION@#Long-snake moxibustion can effectively improve the pain, dysfunction and the symptom of cold and dampness in patients with nonspecific low back pain, and the improvement is superior to the ginger-partitioned moxibustion at point.


Subject(s)
Acupuncture Points , Humans , Low Back Pain , Therapeutics , Medicine, Chinese Traditional , Moxibustion
6.
Chinese Journal of Nephrology ; (12): 526-534, 2020.
Article in Chinese | WPRIM | ID: wpr-870989

ABSTRACT

Objective:To explore the development and clinical application value of Nomogram model, a noninvasive early diagnosis model, in IgA nephropathy.Methods:The clinical data of 712 patients with primary glomerular disease diagnosed by renal histopathological examination in Affiliated Hospital of Qingdao University during October 1, 2010 to August 31, 2019 were collected retrospectively, including 241 cases of IgA nephropathy and 471 cases of non-IgA nephropathy. According to the time of case inclusion, the patients were divided into the training set ( n=426, 156 cases of IgA nephropathy and 270 cases of non-IgA nephropathy) and the validation set ( n=286, 85 cases of IgA nephropathy and 201 cases of non-IgA nephropathy). Univariate and multivariate logistic regression equations were used to analyze the risk factors for diagnosing IgA nephropathy in patients of training set. Nomogram model for noninvasive diagnosis of IgA nephropathy was established according to the akichi information criteria (AIC) and applied to the validation set for validation. The discriminant degree, calibration degree and clinical practicability of the model were verified and evaluated by receiver operating characteristic curve (ROC), calibration curve and decision curve analysis (DCA), respectively. Results:Multivariate logistic regression results showed that the age ( OR=0.966, 95% CI 0.947-0.985, P=0.001), IgA/C3 ratio ( OR=1.889, 95% CI 1.468-2.432, P<0.001), serum albumin ( OR=1.091, 95% CI 1.047-1.136, P<0.001), total cholesterol ( OR=0.810, 95% CI 0.694-0.946, P=0.008), and gross hematuria ( OR=6.858, 95% CI 1.867-25.189, P=0.004) of patients with primary glomerular disease were independent factors for the diagnosis of IgA nephropathy. Nomogram diagnostic model was constructed based on the above indicators, and the areas under ROC curve were 0.880 and 0.887 respectively in the training set and the validation set. The calibration curve showed that the predicted probability of the model was in good agreement with the actual probability. DCA showed that the safety and clinical net benefit of the model were higher. Conclusions:The Nomogram model has high accuracy and clinical practicality in diagnosing IgA nephropathy, and can be used for noninvasive and early diagnosis of IgA nephropathy to enable patients to receive early treatment.

7.
Article in Chinese | WPRIM | ID: wpr-865694

ABSTRACT

Objective:To analyze the risk factors for severe acute pancreatitis (SAP) complicated with infection and the effects on immune level.Methods:A total of 150 SAP patients admitted to Deyang People′s Hospital from February 2018 to April 2019 were divided into the infected group ( n=90) and the uninfected group ( n=60) according to whether SAP was complicated with infection or not; the changes of pathogenic bacteria in the infection focus, infection risk factors, blood inflammatory cytokines levels and T-lymphocyte subgroups were analyzed. Results:A total of 105 pathogenic bacteria were detected in 90 SAP patients with infection, among which 74(70.5%) were gram-negative bacteria, mainly escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa. There were 27 strains (25.7%) of gram-positive bacteria, mainly staphylococcus aureus, and 4 strains (3.81%) of fungi. Biliary causes, total parenteral nutrition time≥1 week, APACHEⅡ score≥11, surgical intervention, and respiratory mechanical ventilation were all independent factors for SAP infection (all P<0.05). At 24 hours after onset, blood IL-4(59.1±6.2)ng/L, IL-6(134.1±12.2) ng/L, IL-10(146.4±13.2)ng/L, TNF-ɑ(76.3±5.2)ng/L in infected group were all significantly higher than those in the uninfected group (all P values <0.05); at 30 days after the onset, blood IL-4(33.6±5.8)ng/L, IL-6(49.2±6.8)ng/L of the infected group, IL-10(80.7±8.8)ng/L, TNF-ɑ(28.7±5.5)ng/L in infected group were significantly lower than those in the uninfected group (all P values <0.05). At 24 hours after onset, the proportion of CD 4+ T lymphocytes in the infected group was significantly higher than that in the uninfected group [(45.3±5.5)% vs (32.3±5.2)%], and the proportion of CD 8+ T lymphocytes was significantly lower than that in the uninfected group [(20.6±4.2)% vs (29.7±4.8)%]; at 30 days after onset, the proportion of CD 4+ T lymphocytes in the infected group was significantly lower than that in the uninfected group [(21.6±3.7)% vs (40.2±2.5)%], and the proportion of CD 8+ T lymphocytes was significantly higher in the uninfected group [(48.4±4.1)% vs (32.8±4.0)%]; and all the differences were statistically significant (all P <0.05). Conclusions:The strains of concurrent infection with SAP were mainly gram-negative bacteria. Biliary causes, total parenteral nutrition time, surgical intervention and respiratory mechanical ventilation were all risk factors for concurrent infection with SAP. SAP infection may cause excessive inflammatory response and lead to immune cell damage, which should be paid attention to in clinical treatment.

8.
Article in Chinese | WPRIM | ID: wpr-863919

ABSTRACT

Objective:To assess the impact of timing of percutaneous catheter drainage (PCD) application (ANC and WON) on outcomes and complications in severe acute pancreatitis (SAP) treatment.Methods:The clinical data of 75 SAP patients treated with PCD from Jan. 1, 2018 to Dec.31, 2018 in Pancreatitis Treatment Center of Deyang People’s Hospital were retrospectively analyzed. The clinical manifestations, application period and curative effect of PCD were analyzed.Results:Among the 75 patients, 51 (68%) were ANC patients, (32%) were WON patients, 0 (0%) died, 70 (93.3%) were cured, and 5 (6.7%) underwent further surgical treatment. There was no significant difference between the average time of PCD application, or complications (such as pancreatic fistulae, catheter obstruction or falling and haemorrhage) ( P>0.05) . Conclusion:Percutaneous catheter drainage in different stages of acute necrotizing pancreatitis does not affect its safety and efficacy, and delayed use don’t have better curative effect.

9.
Article in Chinese | WPRIM | ID: wpr-744194

ABSTRACT

Education of professional postgraduate medical students is an important way for the cultivation of high-level medical and health talents in China.This article discusses the current status and problems in the training of professional postgraduate medical students,with thoughts on how to train qualified professional postgraduate medical students majoring in urology in the context of the expansion of enrollment scale.This article also puts forward the following detailed suggestions:pay attention to the quality of students and formulate corresponding training strategies;continuously improve the teaching level of postgraduate tutors and teachers and attach importance to the quality of teaching;improve the rotation and assessment system for postgraduates;actively apply new training methods.

10.
Article in Chinese | WPRIM | ID: wpr-772676

ABSTRACT

OBJECTIVE@#This study aims to evaluate the effect of Angelica sinensis polysaccharide (ASP) on the osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) of rats with high glucose levels.@*METHODS@#Rat BMSCs were isolated and identified by osteogenic and adipogenic differentiation. Then, the BMSCs were divided into three groups as follows: normal control group (5.5 mmol·L⁻¹ glucose), high glucose group (25.5 mmol·L⁻¹ glucose), and ASP+high glucose group (25.5 mmol·L⁻¹ glucose +40 mg·L⁻¹ ASP). The proliferation activities of the BMSCs were detected by CCK8. Alizarin red staining, and alkaline phosphatase activity were used in the examination of osteogenic activity. Quantitative real time-polymerase chain reaction was used to detect the expression levels of the osteogenic genes (Runx2, Osx, OCN, Col-Ⅰ) and the key factors of Wnt/β-catenin signal pathway (CyclinD1, β-catenin). In vivo, a type 2 diabetes rat model was established. The rats were divided into three groups, namely, the normal control group (normal rats), diabetes group (diabetic rats), diabetes+ASP group (diabetic rats, ASP feeding). Then, the tibia bone defect was established. The repair of bone defects in each group was observed through histological examination.@*RESULTS@#The proliferation of BMSCs was higher in the high glucose group and ASP+high glucose group than in the normal control group (P0.05). The number of calcium nodules of BMSCs; alkaline phosphatase activity; and the mRNA expression of Runx2, OCN, Osx, Col-Ⅰ, CyclinD1, β-catenin in the high glucose group were lower than those in the normal control and ASP+high glucose groups (P0.05). The bone mass was significantly lower in the bone defect of the diabetes group than in the bone defect of the normal control or diabetes+ASP group (P0.05).@*CONCLUSIONS@#ASP can promote the osteogenic differentiation of rat BMSCs under high glucose culture and induce bone regeneration in rats with type 2 diabetes. These features may be related to the activation of the Wnt/β-catenin signaling pathway.


Subject(s)
Angelica sinensis , Chemistry , Animals , Bone Marrow Cells , Cell Differentiation , Cells, Cultured , Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 2 , Glucose , Mesenchymal Stem Cells , Osteogenesis , Plant Extracts , Pharmacology , Polysaccharides , Pharmacology , Rats
11.
Article in Chinese | WPRIM | ID: wpr-789159

ABSTRACT

Objective To observe the clinical effect of "palace bone-setting" Dieda-Wanying cream combined with Yuan Shu Zhi Pai Zi fixation in the treatment of acute ankle sprain.Methods A total of 60 patients with acute ankle sprain were collected and randomly divided into observation group and control group according to the random number table method,with 30 cases in each group.The observation group was treated with "Palace Bone-setting" Dieda-Wanying cream combined with Yuan Shu Zhi Pai Zi for fixation.The control group was treated with external application of diclofenac diethylamine emulsion and fixation of elastic bandage.Two groups were treated for 14 days.The patients pain disappeared time and the swelling subsided,the VAS scale for assessment of ankle pain observed.The American Orthopedic Foot and Ankle Society (AOFAS) Ankle-after scoring system was used to assess the Ankle function,evaluation of clinical curative effect.Results The pain disappear time (9.7 ± 1.6 d vs.13.4 ± 3.5 d,t=5.638),regression and time (3.5 ± 1.3 d vs.6.7 ± 1.1 d,t=10.292) in the observation group were significantly earlier than those in the control group (P<0.01).After treatment,VAS score (1.9 ± 1.1 vs.3.3 ± 1.3,t=4.503) of the observation group significantly lower than that of the control group (P<0.01);AOFAS score (93.6 ± 3.9 vs.84.2 ± 5.1,t=8.019) significantly higher than the control group (P<0.01).Total effective rate in the observation group was 96.7% (29/30),and the control group was 80.0% (24/30),which the difference was statistically significant (x2=4.043,P=0.044).Conclusions The "Palace Bone-setting" Dieda-Wanying cream combined with Yuan Shu Zhi Pai Zi fixation has definite curative effect on acute ankle sprain,relieve pain,swelling and promote the ankle function.

12.
Chinese Journal of Rheumatology ; (12): 543-547, 2019.
Article in Chinese | WPRIM | ID: wpr-791345

ABSTRACT

Objective This study aims to investigate the variation of neutrophil extracellular traps (NETs) in animal model of staphylococcus aureus (SA) infected systemic lupus erythematosus (SLE) MRL/Lpr mouse.Methods The infection model was developed by injecting the Staphylococcus Aureus in the mouse tail vein.The Staphylococcus Aureus load of the serum and kidney was detected by enzyme-linked immuno sorbent assay (ELISA).The level of NETs complex in the serum was detected by Cell-Death-Elisa-Plus-Kit and anti-myeloperoxidase (MPO) antibody.The kidney NETs formation was tested with the immunofluorescence.Statistical program for social sciences (SPSS) 17.0 software and Image Pro Plus statistical software were used for analysis.Comparisons between groups were made using unpaired t test for normally distributed numerical data,nonparametric test for non-normally distributed numerical data,and single factor analysis (ANOVA) for variance.Results The incidence of walking instability,purulent exudate in eyes and death was 2/6,1/6,1/6 respectively in Staphylococcus Aureus-infected MRL/Lpr mice,However,The control group had no death and related symptoms.The Staphylococcus Aureus load of the serum was higher in MRl/lpr mice when compared to the control group [(106.79±23.39) ng/ml vs (48.82±11.49) ng/ml,U=2.739,P<0.05] after Staphylococcus Aureus infection.The control group had a higher NETs level [(2.24±0.15) vs (1.64±0.08),U=2.882,P<0.05],however,all the MRL/Lpr mice had no significant change of the NETs level [(2.63±0.61) vs (2.65 ±0.260),U=0.548,P>0.05] after Staphylococcus Aureus infection.Conclusion After infected by the Staphylococcus Aureus,more symptoms,higher death rate and higher Staphylococcus Aureus load of the serum present in the MRlL/lpr mice than the control group.While,there's none significant change of the NETs level in Staphylococcus Aureus-infected MRLL/lpr mice.This suggests that the dyspoiesis of NETs is present in Staphylococcus Aureus-infected MRL/lpr mice.

14.
Article in Chinese | WPRIM | ID: wpr-856684

ABSTRACT

Objective: To investigate the effectiveness of Ilizarov technique in treatment of rigid congenital clubfoot in older children. Methods: A clinical data of 55 patients (69 feet) with rigid congenital clubfoot (Diméglio grade Ⅳ) between September 2005 and September 2015 were retrospectively analyzed. There were 33 boys and 22 girls with an average age of 11 years (range, 6-14 years). There were 41 cases of unilateral clubfoot and 14 cases of bilateral clubfeet. The degree of foot deformity was rated as fair in 24 feet and as poor in 45 feet according to International Clubfoot Study Group (ICFSG) score before operation. Sixty-nine feet were treated by Ilizarov external fixator combined with limited soft tissue release, and 21 feet were combined with individualized V-shaped osteotomies. Results: All patients were followed up 2-10 years ( mean, 4.2 years). The incisions healed well after operation. Five cases of needle tract infection occurred and were cured by local dressing change. The effectiveness was excellent in 39 feet, good in 21 feet, and fair in 9 feet according to ICFSG score, with the excellent and good rate of 87.0%. During the follow-up, X-ray films showed that no blood supply disorder or necrosis of talus, scaphoid, cuneiform, metatarsal, calcaneus, and cuboid occurred. No osteomyelitis occurred. Conclusion: Ilizarov technique combined with limited soft tissue release or V-shaped osteotomy for the older children with rigid congenital clubfeet is safe and can obtain significant orthopedic effectiveness.

15.
Chinese Journal of Nephrology ; (12): 295-301, 2018.
Article in Chinese | WPRIM | ID: wpr-711112

ABSTRACT

Objective To investigate the possible effects of histone methyltransferase MLL1 on renal interstitial fibrosis and epithelial-mesenchymal transition(EMT).Methods Forty-two male SD rats were randomly divided into normal group,sham operation group and unilateral ureteral occlusion(UUO)group,and then UUO group was further divided into group 1 d,1 week,2 week,3 week and 4 week after operation.The expression of MLL1,E-cadherin,α-SMA,Vimentin and Col3α1 in UUO rat kidney tissue as well as TGF-β1 stimulated HK-2 cells were detected by real-time PCR and Western blotting.siRNA-MLL1 plasmids was used to inhibit the expression of MLL1 and the protein levels of MLL1,α-SMA,Vimentin,E-cadherin,Col3α1 and H3K4me3 induced by TGF-β1 stimulation were detected by Western blotting.The level of H3K4me3 in promoter region of EMT related genes was observed by chromatin immunoprecipitation(CHIP).Results Compared with normal and sham operated groups,the loss of renal function in UUO group was more obvious with the obstruction time(P < 0.05).The renal fibrosis was most obvious 1 week and 2 weeks after the rats underwent the UUO operation(all P < 0.05),with the highest protein expressions of MLL1,E-cadherin,α-SMA,Vimentin and Col3α1(all P < 0.05).Compared with the control group,3 ng/ml TGF-β1 induced the highest expression of MLL1 and the most obvious EMT in HK-2 cells(all P < 0.05).Moreover,the EMT and the high level of H3K4me3 in HK-2 triggered by TGF-β1 were all inhibited by siRNA-MLL1 plasmids transfection(all P < 0.05).Conclusions MLL1 can enhance the occurrence of EMT induced by TGF-β1 in HK-2 cells by increasing the level of H3K4me3 in the promoter region of α-SMA and Vimentin.

16.
Article in Chinese | WPRIM | ID: wpr-703562

ABSTRACT

Objective:To investigate the quality of 5-year medical undergraduates in China,analyze the influ-ence factors and put forward relevant policy suggestions. Method:By stratified random sampling,there are 24 medi-cal colleges or universities in northeastern, eastern, central, southern and southwestern China were included. Ac-cording to access the official websites,the enrollment data of 5-year medical undergraduates were collected. Result:The percentile of medical students'average score in entrance examination was around 33.46% ~56.32% in the first batch. There are significant difference among areas about the quality of medical undergradnates. Conclusion:At pres-ent,the quality of the 5-year medical undergraduates is good. Because high quality students unwilling to apply for clinical medicine,the quality of students declined. For this question,the suggestions are to improve enrollment sta-tus,school finical support,medical environment and doctors' welfare.

17.
Article in Chinese | WPRIM | ID: wpr-695810

ABSTRACT

Objective To analyze the imaging findings in MiT family translocation renal cell carcinoma (RCC) for the better understanding on the rare tumor combined with its clinicopathological features.Methods Fifteen patients with MiT family translocation RCC were analyzed retrospectively including 14 cases of Xp11.2 RCC and 1 case of t(6;11) RCC.Tumor location,size,capsule sign,hemorrhage,cystic change,calcification,density,signal features and enhancement pattern were evaluated.Results Four men and ten women aged from 14 to 42 years old (mean age of 26 years old) were diagnosed as Xp1 1.2 RCC.Most of the tumors (n =12) were located in renal medulla and most of the tumors (n =11) were 4 cm or more in diameter.There were 8 cases with hemorrhage.They were always well-defined (n =11) and all heterogeneous.Additionally,we could find calcification (a half of the number) typically distributed on CT.The tumor density was grater on plain CT (n =8) and the signal intensive was slightly high on T1WI (n-6) and low or slightly low on T2WI (n =9) than renal cortex.All of the cases were slightly or moderately persistent enhancement on DCE CT and MRI.The imaging features of the male patient with t(6;11)RCC was similar with Xp11.2 RCC apart from the diameter of 16 cm.Conclusions MiT family translocation RCC should be considered when a young patient presents with a relatively huge and well-defined renal tumor with an obviously heterogeneous features,annular calcification and persistent enhancement.It is difficult to distinguish Xp11.2 RCC and t(6,11) RCC,but size maybe play a hinting role in discriminate them.

18.
Article in Chinese | WPRIM | ID: wpr-695569

ABSTRACT

Objective To investigate the protection of parathyroid glands and their functions during reoperation of thyroid carcinoma.Methods The clinical data of 39 patients who underwent reoperation of thyroid carcinoma from Jan.2012 to Mar.2017 in our hospital were studied retrospectively.They were divided into two groups (group A:17 patients who underwent reoperation within 3 months from initial operation;group B:22 patients who underwent reoperation 3 months or longer since the first operation).The numbers of parathyroid including inadvertent parathyroidectomy (IPE) and ischemic parathyroid,and the dyeing conditions in the two groups were observed and recorded respectively.Serum calcium and parathyroid hormone (PTH) levels were determined after operation.Results In group A,10 cases with IPE(7 parathyroid glands were found intraoperative dissection specimens,3 parathyroid glands postoperatieve pathology confirmed),4 parathyroid glands in situ were found ischemia;In group B,4 cases with IPE (4 parathyroid glands were found intraoperative dissection specimens),3 parathyroid glands in situ were found ischemia.All of IPE found through intraoperative dissection and ischemic parathyroid glands were transplanted.A total of 11 parathyroid glands were transplanted in group A(7 cases with 1 parathyroid transplantation and 2 cases with 2 parathyroid transplantation).The number of parathyroid gland transplants in group B was 7 (5 cases with 1 parathyroid transplantation and 1 case with 2 parathyroid transplantation).The rate of IPE in the group A was 58.8%(10/17),significantly higher than 18.2%(4/22) in the group B (P<0.05);The rate of temporary hypoparathyroidism in the group A was 52.9% (9/17),significantly higher than in the group B 18.2%(4/22) (P<0.05);With the follow-up of 3~6 months after surgery,no case with permanent hypoparathyroidism.Conclusion Select the appropriate reoperation time combined the nano-carbon negative development during the reoperation of thyroid carcinoma,can reduce IPE;necessary parathyroid transplantations are effective measures to avoid the postoperative permanent hypoparathyroidism.

19.
Article in Chinese | WPRIM | ID: wpr-695069

ABSTRACT

Purpose To observe the expressions of LC3, NY-ES0-2, MAGE-D4, CD4+, CD8+, CD68+ in colorectal cancer and normal tissues and analysis the correlation of autophagy related gene LC3 and tumor surface antigen NY-ES0-2, MAGE-D4, immune cells CD4+, CD8+, CD68 +. To investigate the effect of the change of autophagy on immune cells function and its clinical significance. Methods Immunohistochemistry and Western blot were used to detect the expressions of LC3, NY-ESO-2, MAGE-D4, CD4, CD8, CD68 in 128 cases of colorectal cancer and normal tissues. The correlation among each factors and the patients' clinicopathological features were analyzed. Results (1 ) The expression of LC3 in colorectal cancer tissue was higher than in normal tissues. The expression of NY-ESO-2 was low while the expression of MAGE-D4 was high in colorectal cancer and both almost not express in normal tissues(P<0.05). The infiltration of CD4+, CD8 +, CD68+ immune cells in colorectal cancer were higher than in normal tissues(P<0.05). (2)The expression of LC3 protein in colorectal cancer was correlated positively with the expressions of tumor surface antigen NY-ESO-2, MAGE-D4 protein and the infiltration of CD8 +, CD68 + immune cells (P< 0.05 ), but had no correlation with the infiltration of CD4 + immune cells (P>0.05). (3 ) The expression of NY-ESO-2 was correlated positively with the infiltration of CD4+, CD8 +, CD68 + immune cells. The expression of MAGE-D4 was correlated positively with the infiltration of CD8 +, CD68+ immune cells. (4) The expressions of NY-ESO-2, MAGE-D4, the infiltration of CD4+, CD8 + immune cells and lymph node metastasis were negatively correlated (P<0.05). The expressions of NY-ESO-2, MAGE-D4, the infiltration of CD4+, CD8+ immune cells and TNM stage were negatively correlated (P< 0.05). The infiltration of CD8 + immune cells and grade was positively correlated (P<0.05). Conclusion The expression of autophagy-related gene LC3 was related to the expressions of tumor surface antigen NY-ESO-2, MAGE-D4 and the infiltration of immune cells CD8 + and CD68+ in colorectal cancer. Therefore the autophagy key factor LC3 may participate in the immune of colorectal cancer.

20.
Article in Chinese | WPRIM | ID: wpr-694239

ABSTRACT

Objective To analyze the risk factors that affect the early recurrence (recurrence occurring within 3 months after surgical resection) of hepatocellular carcinoma (HCC), and to discuss the risk factors influencing the survival after hepatectomy. Methods The clinical data of 257 HCC patients, who were admitted to authors' hospital during the period from January 1, 2007 to March 31, 2014 to receive cTACE within 3 months after surgical resection of hepatocellular carcinoma, were retrospectively analyzed. According to DSA findings (lipiodol CT scan was performed in part of patients with undetermined diagnosis), the patients were divided into recurrence group and non-recurrence group. By using univariate analysis and multiple logistic regression analysis, the correlation of the clinical and pathological data with the early recurrence was analyzed. The patients were followed up, the survival time was recorded. The relationship between patient's clinical data and postoperative survival was evaluated. Results ① Of the 257 patients, early recurrence was detected in 150 patients (58. 4%, recurrence group) and no recurrence was observed in 107 patients (41. 6%, non-recurrence group). ②The presence of satellite nodules and the integrity of tumor encapsulation were two independent factors associated with the postoperative residual lesions. ③The maximum diameter of the tumor, Edmondson grade and the vascular cancer thrombus were the independent risk factors affecting survival. ④The median survival time of patients in recurrence group was markedly shortened than that of patients in non-recurrence group (39 months vs. 93 months). Conclusion The early recurrence (within 3 months after resection) of hepatocellular carcinoma is associated with the presence of satellite nodules and the integrity of tumor encapsulation. The survival of patients after hepatectomy is related to the maximum diameter of the tumor, Edmondson grade and the vascular cancer thrombus. The median survival time in patients having early recurrence is significantly shortened than that in patients having no early recurrence. (J Intervent Radiol, 2018, 27: 215-222)

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