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1.
Article in English | WPRIM | ID: wpr-879669

ABSTRACT

Traumatic brain injury (TBI), a growing public health problem, is a leading cause of death and disability worldwide, although its prevention measures and clinical cares are substantially improved. Increasing evidence shows that TBI may increase the risk of mood disorders and neurodegenerative diseases, including Alzheimer's disease (AD). However, the complex relationship between TBI and AD remains elusive. Metabolic dysfunction has been the common pathology in both TBI and AD. On the one hand, TBI perturbs the glucose metabolism of the brain, and causes energy crisis and subsequent hyperglycolysis. On the other hand, glucose deprivation promotes amyloidogenesis via β-site APP cleaving enzyme-1 dependent mechanism, and triggers tau pathology and synaptic function. Recent findings suggest that TBI might facilitate Alzheimer's pathogenesis by altering metabolism, which provides clues to metabolic link between TBI and AD. In this review, we will explore how TBI-induced metabolic changes contribute to the development of AD.

2.
Article in Chinese | WPRIM | ID: wpr-885155

ABSTRACT

Objective:To explore the clinical characteristics of patients with pituitary thyrotropin-secreting adenoma and evaluate the effect of preoperative short-acting octreotide treatment on hyperthyroidism.Methods:A retrospective analysis was performed in 40 patients with pituitary thyrotropin adenoma diagnosed in Beijing Tiantan Hospital from January 2008 to January 2018. The general data, laboratory examinations and imaging findings were reviewed and analyzed. The clinical effect of preoperative octreotide on hyperthyroidism was evaluated.Results:The age of onset year of the 40 patients (male: female = 24∶16) was (30.5±5.1) years. Among them, 35 patients (87.5%) were with macroadenoma. The most common symptoms were thyroid hypermetabolism syndrome, followed by headache, dizziness, visual field damage and hypogonadism. The thyroid function of 30 patients (75%) recovered to normal within 3-5 days after the octreotide treatment. The total effective rate of the octreotide was 90.0%. The level of free thyroxine (FT 4) before treatment in patients with more than 10 times of effective cumulative dose was significantly higher than that in patients with less than 10 doses. Conclusions:Thyroid hypermetabolism syndrome and pituitary occupying effect are the most common clinical manifestations of thyrotropin-secreting adenoma. Preoperative octreotide treatment can effectively control hyperthyroidism. The level of FT 4 is a crucial factor affecting the efficacy of octreotide.

3.
Article in Chinese | WPRIM | ID: wpr-873582

ABSTRACT

@#To investigate the effect of ethanolic extract from Artemisia Argyi Folium on blood glucose and blood lipids in diabetic mice, ICR mice were induced by intraperitoneal injection of 35 mg/kg streptozotocin (STZ) and a high-carbohydrate/high-fat diet to construct type 2 diabetes mellitus model. Diabetic mice were randomly divided into three groups: the model group (5 mL/kg 0.5% CMC-Na), the Artemisia Argyi Folium ethanolic extract low-dose group (100 mg/kg ) and high-dose group (400 mg/kg ). During the treatment for 6 weeks, the amount of drinking water and food intake of mice were recorded every day. Blood glucose and body weight were recorded every week. After treatment for 6 weeks, serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C),and oral glucose tolerance (OGTT) were measured. The results showed that the amount of drinking water and food intake of mice significantly decreased (P < 0.01) in the Artemisia Argyi Folium ethanolic extract high-dose group; oral glucose tolerance was significantly improved (P < 0.01) and the contents of TC, TG and LDL-C were significantly decreased in the Artemisia Argyi Folium ethanolic extract low-dose group (P < 0.01). The ethanolic extract from Artemisia Argyi Folium could significantly improve the glucose and lipid metabolic disorder in T2DM mice in a dose-dependent manner.

4.
Article in Chinese | WPRIM | ID: wpr-879436

ABSTRACT

OBJECTIVE@#To explore the treatment strategy and clinical efficacy for os odontoideum complicated with atlantoaxial dislocation.@*METHODS@#The clinical data of 17 patients with os odontoideum complicated with atlantoaxial dislocation surgically treated from January 2006 to January 2015 were retrospectively analyzed, including 7 males and 10 females, aged 17 to 53 (43.1±11.3) years old;course of disease was 3 to 27(10.2±6.9) months. All patients received cranial traction before operation, 12 of 14 patients with reducible dislocation were treated by posterior atlantoaxial fixation and fusion, and 2 patients with atlantooccipital deformity were treated by posterior occipitocervical fixation and fusion;3 patients with irreducible alantoaxial dislocation were treated by transoral approach decompression combined with posterior atlantoaxial fixation and fusion. The operation time, intraoperative blood loss and perioperative complications were recorded. Visual analogue scale (VAS) and Japanese Orthopaedic Association (JOA) score were used to evaluate the change of neck pain and neurological function. Atlantoaxial joint fusion rate was evaluated by CT scan.@*RESULTS@#The operation time of posterior fixation and fusion ranged from 86 to 170 (92.2±27.5) min, and the intraoperative blood loss was 200-350 (250.7±65.2) ml. No vertebral artery injury and spinal cord injury were recorded. Among the patients underwent atlantoaxial fixation and fusion, 1 patient with reducible dislocation fixed by C@*CONCLUSION@#Surgical treatment of os odontoideum complicated with atlantoaxial dislocation can achieve satisfactory results, improve the patient's neurological function and improve the quality of life, however the surgical options needs to be individualized.


Subject(s)
Adolescent , Adult , Atlanto-Axial Joint/surgery , Axis, Cervical Vertebra , Female , Humans , Joint Dislocations/surgery , Male , Middle Aged , Quality of Life , Retrospective Studies , Spinal Fusion , Treatment Outcome , Young Adult
5.
Article in Chinese | WPRIM | ID: wpr-879420

ABSTRACT

OBJECTIVE@#To investigate the clinical efficacy and superiority of direct lateral interbody fusion combined with posterior percutaneous screw fixation in the treatment of lumbar tuberculosis.@*METHODS@#From June 2013 to August 2016, the clinical data of 83 patients with lumbar tuberculosis were retrospectively analyzed, including 55 males and 28 females, aged from 27 to 72 (49.5±13.5) years. These 83 patients were divided into two groups according to different operation methods, 35 cases in group A were treated with direct lateral interbody fusion combined with posterior percutaneous screw fixation;48 cases in group B were treated with anterior traditional extraperitoneal debridement combined with posterior internal fixation. After operation, regular quadruple antituberculosis drugs were continued for 18 months. The operation time, intraoperative blood loss, hospital stay, bone graft fusion time and complications were compared between the two groups. Visual analogue score (VAS) of lumbar pain, Oswestry Disability Index (ODI), sagittal Cobb angle, erythrocyte sedimentation rate (ESR) and C-reactive protein(CRP) values before and after operation were analyzed.@*RESULTS@#The operation was successfully completed in both groups, and the operation mode was not changed during operation. The operation time, intraoperative blood loss and hospital stay were (149.4±13.3) min, (354.3±69.0) ml, (9.4±1.6) d in group A and(116.8±10.0) min, (721.9±172.3) ml, (11.8±1.7) d in group B, respectively, with significant difference between the two groups (@*CONCLUSION@#The two kinds of operation can obtain satisfactory clinical effect. Direct lateral interbody fusion combined with posterior percutaneous screw fixation can reduce intraoperative blood loss and hospital stay, which is conducive to early rehabilitation of patients.


Subject(s)
Aged , Bone Transplantation , Debridement , Female , Humans , Lumbar Vertebrae/surgery , Male , Pedicle Screws , Retrospective Studies , Spinal Fusion , Thoracic Vertebrae , Treatment Outcome , Tuberculosis, Spinal/surgery
6.
Article in Chinese | WPRIM | ID: wpr-879391

ABSTRACT

OBJECTIVE@#To investigate relationship between cold pain of knee joint and subchondral bone marrow edema (BME).@*METHODS@#From May 2018 to August 2019, 92 patients with knee osteoarthritis (KOA) associated with cold pain of knee were admitted, all patients were underwent MRI examination. The patients were divided into observation group (47 patients with BME) and control group(45 patients without BME). In observation group, there were 6 males and 41 females aged from 36 to 87 years old with an average of (63.2±12.3) years old. In control group, there were 10 males and 35 females, aged from 48 to 84 years old with an average of (62.7±8.3) years old. All patientswere treated with drugs. The degree of joint degeneration was evaluated by Kellgren-Lawrence (K-L) grading. Degree of cold pain of knee was evaluated by knee cold pain score, and degree of BME was evaluated according to WORMS. The correlation between cold pain of knee and K-L grading and BME was analyzed.@*RESULTS@#Score of cold pain in observation group (15.55±7.68) was higher than that of control group (9.42± 5.50), which had significant difference (@*CONCLUSION@#The cold pain of KOA patients is not related to K-L grading, but corelate with BME grading. The Cold pain of knee was more pronounced in KOA patients with BME, and the severity of BME is often related to degree of cold pain. It seemed to be a tendency:the more serious BME, the heavier coldpain.


Subject(s)
Adult , Aged , Aged, 80 and over , Bone Marrow , Edema , Female , Humans , Knee Joint/diagnostic imaging , Magnetic Resonance Imaging , Male , Middle Aged , Osteoarthritis, Knee/diagnostic imaging , Pain/etiology
7.
Article in English | WPRIM | ID: wpr-879307

ABSTRACT

Objective To assess the effects of vascular endothelial growth factor-C (VEGF-C)/vascular endothelial growth factor receptor-3 (VEGFR-3) signaling on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in human osteosarcoma MG63 cells and the subsequent impact on the proliferation of human umbilical vein endothelial cells (HUVECs). MethodsMG63 cells were treated with VEGF-C alone (VEGF-C group), VEGF-C + iNOS inhibitor aminoguanidine (AG; AG group), and VEGF-C + VEGFR-3 inhibitor MAZ51 (MAZ51 group); untreated MG63 cells were used as controls. NO production was evaluated by a colorimetric method involving nitrate reductase. Meanwhile, mRNA and protein levels of iNOS were examined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. To explore the effect of VEGF-C/VEGFR-3/iNOS signaling of MG63 cells on proliferation of HUVECs, we set up six groups: HUVECs, HUVECs+MG63, HUVECs+VEGF-C, HUVECs+MG63+VEGF-C, HUVECs+MG63+VEGF-C+AG, and HUVECs+MG63+VEGF-C+MAZ51 groups. The proliferation of HUVEC cells was assessed by Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay, and proliferating cell nuclear antigen (PCNA) expression quantitation. ResultsVEGF-C treatment enhanced iNOS expression at both gene and protein levels (mRNA: LSD-

8.
Article in English | WPRIM | ID: wpr-879306

ABSTRACT

Objective To characterize Chinese families in which both parents and at least one child are diagnosed with malignant diseases and provide reference for cancer screening or early detection in people whose both parents are diagnosed with cancer. Methods Medical records of all clients to the center of cancer screening and prevention of the National Cancer Center/Cancer Hospital between January 2008 and February 2018 were screened to select families in which both parents and at least one child were diagnosed with malignant diseases. The cancer profiles of fathers, mothers, sons and daughters, their age distribution at diagnosis, and similarity of cancers between two generations were analyzed. The proportions of each cancer in males and females of the cohort were compared with corresponding data from the National Cancer Center Registry of China (NCCRC) in 2013. Results Totally 135 families were identified from records of 33 200 clients. Proportion of lung cancer in fathers (40/135, 29.6%) and in mothers (38/135, 28.1%) were higher than the national data (23.9% in males and 14.9% in females, respectively). The proportion of breast cancer in daughters (35/109, 32.1%) was higher than that of mothers (14/135, 10.4%) and the national data (17.1%). In 71 father-son pairs of cancer, 46.5% (33/71) were of the same systematic disease, and 16.9% (12/71) were of the same cancer. These two indexes were 31.2% (

9.
Organ Transplantation ; (6): 77-2021.
Article in Chinese | WPRIM | ID: wpr-862779

ABSTRACT

Objective To investigate the clinical characteristics and the experience of multi-disciplinary team (MDT) on recurrence of primary hyperoxaluria (PH) type I after renal transplantation. Methods One case presenting with unexplained rapid decline of renal allograft function after allogeneic renal transplantation was discussed by MDT. The role of MDT in diagnosing rare hereditary diseases and improving the long-term survival of renal transplant recipients was summarized. Results After MDT consultation, the patient was diagnosed with recurrence of PH type I. Routine immunosuppressive regimen was initiated after the exclusion of rejection. The patient was instructed to drink a large quantity of water, and given with high-quality protein and low-phosphorus diet, vitamin B6, calcium and other conservative therapies to actively prevent and treat postoperative complications. The deterioration of renal graft function was delayed. Nevertheless, regular hemodialysis was resumed at 5 months after renal transplantation until the submission date of this manuscript. Conclusions Recurrence of PH type I after renal transplantation is relatively rare. The main clinical manifestations are recurrent kidney stones and decreased renal function with multiple complications and poor prognosis. The condition of the patient is consulted by MDT for confirming the diagnosis, determining the optimal treatment scheme, delaying the progression and improving the clinical prognosis.

10.
China Pharmacy ; (12): 158-163, 2021.
Article in Chinese | WPRIM | ID: wpr-862637

ABSTRACT

OBJECTIVE:To establish fingerprint of Huafengdan yaomu ,and to determine the contents of 7 nucleosides in samples of different fermentation time. METHODS :HPLC method was adopted. The determination was performed on Pntulips BP-C18 column with mobile phase consisted of methanol-water (gradient elution )at the flow rate of 0.8 mL/min. The detection wavelength was set at 260 nm,and column temperature was 35 ℃. The sample size was 10 μL. Using xanthine as reference, HPLC fingerprint of 12 batches of Huafengdan yaomu was drawn. The similarity of samples were evaluated with Similarity Evaluation System of TCM Chromatographic Fingerprints (2012 edition). Common peaks were confirmed. The contents of uracil , hypoxanthine,xanthine,uridine,inosine,guanosine and thymidine were determined in samples of different fermentation time (0, 1,2,3,4 weeks)by the same method. RESULTS :There were 8 common peaks in 12 batches of Huafengdan yaomu ,with similarities ranging from 0.712 to 0.954;7 components were identified ,namely uracil ,hypoxanthine,xanthine,uridine,inosine, guanosine and thymidine. The linear ranges of mass concentrations of above 7 components in samples at different fermentation time were 0.87-8.7 μ g/mL (r=0.999 6), 4030 1.51-15.1 μg/mL(r=0.999 7),6.08-60.8 μg/mL(r=0.999 5), 号) 1.52-15.2 μg/mL(r=0.999 6),1.82-18.2 μg/mL(r=0.999 6), 1.48-14.8 μg/mL(r=0.999 6),1.63-16.3 μg/mL(r=0.999 3). The limits of quantification were 0.027 4,0.076 3,0.250 4,0.172 3,0.101 1,0.078 3,and 0.084 2 μ g/mL,and the detection limits were 0.008 7,0.025 5,0.007 9,0.084 1,0.035 7,0.026 9,0.027 5 μg/mL,respectively. RSDs of precision , repeatability and stability tests (12 h)were all less than 3%. The sample recovery rates were 94.16%-100.16%(RSD=2.24%,n= 6),93.87%-100.65%(RSD=2.67%,n=6),93.52%-99.66%(RSD=2.30%,n=6),93.67%-98.24%(RSD=1.89%,n=6), 96.00%-102.18%(RSD=1.96%,n=6),94.62%-101.54%(RSD=2.82%,n=6),97.72%-104.56%(RSD=2.97%,n=6). After fermentation for 0-4 weeks,the contents of the above 7 components and total nucleosides were 0.042-0.232,0.027-0.181, 0.039-0.651,0.026-0.225,0.034-0.111,0.009-0.124,0.079-0.099,0.647-1.292 mg/g,respectively. After fermentation for 1-4 weeks,the contents of uracil ,hypoxanthine,xanthine and total nucleosides were significantly increased ,compared with 0 week of fermentation;the contents of uridine ,inosine and guanosine were significantly lower than those in 0 weeks. CONCLUSIONS :The established fingerprint has strong characteristics and simple to operate ,which can be used for the quality control of Huafengdan yaomu;the content determination method is accurate and reliable ,and can be used to simultaneously determine the contents of 7 active nucleosides ;the content of nucleosides in Huafengdan muyao is affected by fermentation time.

11.
Article in Chinese | WPRIM | ID: wpr-847592

ABSTRACT

BACKGROUND: Computer navigation system and orthopedic surgery robot have been developed rapidly in spine surgery in recent years, but the operation effect of computer navigation or navigation robot is still questioned. OBJECTIVE: To compare the difference in robot-assisted and fluoroscopy-guided pedicle screw placement. METHODS: The study included clinical trials published in and outside China from August 2008 to August 2019. The retrieval was performed in the online databases including Embase, PubMed, CNKI, and Wanfang Data. Key words in Chinese were: robot assisted, fluoroscopy guided, pedicle screw, pedicle nail, pedicle screw rod, pedicle internal fixation. Search strategy was: pedicle screw AND robot assisted OR fluoroscopy guided. Key words in English were: Robot assisted, Fluoroscopy guided, Pedicle screw, Pedicle stick, Pedicle screw fixation. Search strategy was: “Pedicle screw” OR “Pedicle stick” OR “Pedicle screw fixation” AND “Fluoroscopy guided” OR “Robot assisted”. After data extraction, statistical software Review Manager 5.3 was used for data analysis. RESULTS AND CONCLUSION: (1) Based on the above search strategy, 357 studies were retrieved. A total of 19 trials were included, containing 17 English studies and 2 Chinese studies. (2) Meta-analysis results displayed that the accuracy of placement of the robot-assisted group was superior to that of the fluoroscopy-guided group [95%CI(1.82, 2.52), P < 0.001]. Number of surgical complications [95%CI(0.25, 0.69), P=0.0006] and revision number [95%CI(0.23, 0.71), P=0.002] were less in the robot-assisted group than in the fluoroscopy-guided group. (3) When evaluating pedicle screw placement, robot-assisted has higher pedicle screw placement accuracy, fewer complications and fewer revisions, and is superior to traditional fluoroscopy-guided technique in accuracy and safety. Considering the expensive price and complex operation steps of navigation robot technology, the selection of specific nail placement method should still follow the principle of individualized treatment.

12.
Article in Chinese | WPRIM | ID: wpr-837834

ABSTRACT

Objective To investigate the in vitro and in vivo relative recovery rates of levofloxacin from microdialysis probes. Methods High-performance liquid chromatography (HPLC) was established for determination of levofloxacin concentrations. Column was Kromasil C18 (150 mm×4.6 mm, 5 μm), the mobile phase consisted of acetonitrile and 10 mmol/L of potassium dihydrogen phosphate solution (15:85), with column temperature being 30 °C, detection wavelength being 294 nm, and injection volume being 10 μL. The relative recovery rates of vascular microdialysis probes in vitro were measured by dialysis and reverse dialysis. The effects of drug concentration and flow rate on probe deliveries were investigated. The in vivo recovery rates of the probe in the jugular vein and prostate were measured by reverse dialysis, and the stability of 12 h recovery rate in vivo was investigated. Results When the concentration of levofloxacin was 0.1-50.0 μg/mL, the linear relationship was good in the HPLC analysis (r = 0.998 7), and its specificity was good. The precision and accuracy of high-, medium-, low-concentration quality control samples, and lower limit of quantitation all met the analysis requirements. In the in vitro relative recovery experiments, there was no statistical difference between the recovery rate and reverse recovery rate of the vascular microdialysis probes (P>0.05). Drug concentrations did not affect in vitro recovery rate. The recovery rate decreased with the increase of flow rate. In vivo, the relative recovery rate of the probe was (48.46±1.94)% in the jugular vein and (13.23±1.44)% in the prostate. The relative recovery rates were stable during 12 h. Conclusion The established HPLC can be used for the determination of levofloxacin microdialysis probe. Reverse dialysis can be used to detect the relative recovery rate of levofloxacin microdialysis probe in vivo.

13.
Article in Chinese | WPRIM | ID: wpr-879925

ABSTRACT

Polycystic ovary syndrome (PCOS) is a common endocrine disease of child-bearing period women and one of the main causes of infertility in women. Pentraxin 3 (PTX3) is a multifunctional protein with a series of biological activities. PTX3 participates in the regulation of insulin secretion and glucose metabolism, ovarian cumulus cell function, inflammatory factor activity, androgen metabolism, lipid absorption and transport, and endothelial cell function, thereby improving insulin resistance, promoting follicular development and ovulation, reducing chronic inflammation, inhibiting androgen levels, improving lipid metabolism abnormalities and preventing atherosclerosis and cardiovascular diseases, thus participating in the occurrence of PCOS and its complications. This article reviews the mechanism of PTX3 in PCOS and its complications, trying to provide new ideas and directions for the study of PCOS pathogenesis and its clinical diagnosis and treatment.


Subject(s)
C-Reactive Protein/metabolism , Child , Female , Humans , Insulin Resistance , Polycystic Ovary Syndrome/physiopathology , Serum Amyloid P-Component/metabolism
14.
China Pharmacy ; (12): 1961-1966, 2020.
Article in Chinese | WPRIM | ID: wpr-825009

ABSTRACT

OBJECTIVE:To c ompare the difference of volatile oil and fatty oil constituents from Cinnamomum migao in different sources. METHODS :The steam distillation method and Soxhlet extraction mothod were used to extract volatile oil and fatty oil from C. migao in different sources respectively ,and the extraction rates were calculated ;GC-MS was used to analyze volatile oils and fatty oils constituents from C. migao in different sources. The compounds were searched and matched through NIST 17,WILEY 275 databases and mass spectrometry computer date system. The relative percentage content of each constituent was calculated by peak area normalization method. RESULTS :The extraction rates of the volatile oils from 4 batches of C. migao in different sources were 3.1%,4.5%,6.2% and 5.5%,respectively;the extraction rates of the fatty oils from C. migao were 6.2%,8.3%,10.5% and 9.4%,respectively. A total of 87 constituents were identified in 4 batches of volatile oils of C. migao in different sources ,of which 104 constituents were separated from S 1,67 were identified ,and the relative percentage content was 90.172%;102 constituents were separated from S2,73 were identified ,and the relative percentage contentwas 88.836%;77 constituents were separated from S 3,57 were identified , with a relative percentage content of 93.972%;87 constituents were separated from S 4,60 were m identified,with a relative percentage content of 95.247% . Among above 87 constituents,48 were monotyloids and their derivat ives,33 were sesquiterpenoids and their derivatives ,4 were aliphatic and 2 were ketones. There were 44 common constituents from the volatile oil of C. migao in different sources ,all of which were terpenoids. The relative percentage content of S 1-S4 were 38.556%,66.776%,88.886% and 90.115%,respectively. Among 44 common constituents ,the relative percentage content of which were all greater than 1% were 1,8-cineole(S2: 6.518%;S4:3.850%;S3:1.655%;S1:1.475%;),4-terpineol(S2:1.591%;S4:1.384%;S3:1.193%;S1:1.182%), α-terpinenol(S3:8.662%;S4:7.173%;S2:6.503%;S1:4.839 %),δ-cadinene(S3:8.597%;S4:5.329%;S2:2.677%; S1:2.547%),elemol(S3:4.781%;S2:4.113%;S1:2.568%;S4:1.897%)and γ-eudesmol(S2:4.061%;S3:2.167%;S1: 1.575%;S4:1.197%). A total of 37 constituents were identified in the 4 batches of fatty oil of the C. migao in different sources , of which 87 constituents were separated from S 1,34 were identified ,and the relative percentage content was 91.072%;69 constituents were separated from S 2,28 were identified ,and the relative percentage content was 90.527%;63 constituents were separated from S 3,23 were identified ,the relative percentage content was 85.297%;71 constituents were separated from S 4,24 were identified ,with relative percentage content of 91.527%. Among above 37 constituents,there were 21 monoterpenes and their derivatives,2 sesquiterpenes,13 aliphatics,and 1 alkane. There were 20 common constituents in fatty oil from C. migao of different sources ,and the relative percentage content in S 1-S4 were 89.667%,89.595%,84.651% and 90.972%,respectively. Among 20 common constituents ,the constituents with relative percentage content greater than 1% were methyl caprate (S4: 59.498%;S1:58.733%;S2:57.552%;S3:26.423%)and methyl dodecanoate (S3:31.434%;S2:26.990%;S1:25.095%; S4:24.334%). CONCLUSIONS :There are differences in volatile oil and fatty oil constituents of C. migao from different sources , and the contents of the same constituent were also different.

15.
Organ Transplantation ; (6): 449-2020.
Article in Chinese | WPRIM | ID: wpr-822922

ABSTRACT

Objective To analyze the incidence and risk factors of de novo malignant tumors in renal transplant recipients. Methods Clinical data of 1 549 renal transplant recipients were retrospectively analyzed, including the basic status, pathological type and incidence rate of patients with de novo malignant tumors after renal transplantation. The survival situation of these patiensts was assessed. And the risk factors of de novo malignant tumors after renal transplantation were identified. Results The incidence rate of de novo malignant tumors in renal transplant recipients was 3.03%(47/1 549). The 47 recipients were (48±12) years old when undergoing renal transplantation, and they were (55±12) years old when diagnosed malignant tumors. The time interval between transplantation and diagnosis was 66 (36, 100) months. Among the de novo malignant tumors, colorectal cancer was the most common, with a cumulative incidence rate (CIR) of 0.58%. The survival time of 47 recipients with de novo malignant tumors after renal transplantation was 59 (2, 135) months, and the 5-year survival rate was 50%. The recipients with the age > 45 years old when undergoing renal transplantation was a risk factor for de novo malignant tumors after renal transplantation (P < 0.05). Conclusions The incidence rate of de novo malignant tumors is relatively high in renal transplant recipients. The recipients with the age > 45 years old when undergoing renal transplantation is a risk factor for de novo malignant tumors.

16.
Article | WPRIM | ID: wpr-831107

ABSTRACT

Purpose@#While numerous epidemiological studies have indicated that omega-3 polyunsaturated fatty acids have anticancer properties in various cancers, the effects and mechanisms of eicosapentaenoic acid (EPA) in ovarian cancer cell growth are poorly understood. @*Materials and Methods@#ES2 ovarian clear cell carcinoma cells and SKOV3 adenocarcinoma cells were treated with palmitic acid or EPA, followed by flow cytometry and cell counting to measure apoptosis and proliferation, respectively. A modified protein lipid overlay assay was used to further verify whether EPA was a ligand of G protein–coupled receptor 30 (GPR30) in ES2 cells. The levels of apoptosis-related genes, phosphorylated AKT, and phosphorylated ERK1/2 were detected to explore the underlying mechanism. Finally, inhibitory effect of EPA on tumor growth via GPR30 was determined in vitro and in vivo. @*Results@#EPA suppressed ES2 ovarian clear cell carcinoma cells growth via GPR30, a novel EPA receptor, by inducing apoptosis. As a ligand of GPR30, EPA activated the GPR30-cAMP– protein kinase A signaling pathway. When GPR30 was suppressed by siRNA or its inhibitor G15, the antiproliferative action of EPA was impaired. Furthermore, EPA inhibited tumor growth by blocking the activation of AKT and ERK. In the mouse xenograft model, EPA decreased tumor volume and weight through GPR30 by blocking tumor cell proliferation. @*Conclusion@#These results confirm that EPA is a tumor suppressor in human ovarian clear cell carcinoma cells and functions through a novel fatty acid receptor, GPR30, indicating a mechanistic linkage between omega-3 fatty acids and cancers.

17.
China Pharmacy ; (12): 2574-2578, 2020.
Article in Chinese | WPRIM | ID: wpr-829590

ABSTRACT

OBJECTIVE:To prepar e Celastrol oral u lcer film ,and to evalute its quality primarily. METHODS :The comprehensive scores of the appearance ,film formation and toughness of the drug film were used as indicators ,and the amount of celastrol was controlled to 0.05%. Orthogonal test was used to optimize the amount of excipients as starch ,sodium carboxymethyl cellulose,glycerol and condensed honey ,so as to optimize the formulation ;the validation test was performed. The adhesion force of the film prepared by the optimal formulation were determined. UV spectrophotometer was used to detect the content of celastrol in the film. RESULTS :The optimal dosage of each excipient in Celastrol oral ulcer film was starch 1.0 g,sodium carboxymethyl cellulose 0.2 g,glycerin 0.4 g,condensed honey 1.5 g. In 3 times of validation tests ,the appearance of the prepared film was good. The average adhesion of the film prepared by the optimal formulation was 4.2 g,and the average content of celastrol was 0.135 3 mg/cm2(RSD=1.90%,n=3). CONCLUSIONS :In this study ,the best formulation of Celastrol oral ulcer film was optimized,and the film forming ability of the prepared film is good and the quality is stable and uniform.

18.
China Pharmacy ; (12): 2497-2502, 2020.
Article in Chinese | WPRIM | ID: wpr-829357

ABSTRACT

OBJECTIVE:To establish fingerprint of Lonicera japonica ,and to study its anti-inflammatory spectrum-effect relationship. METHODS :HPLC was adopted. The determination was performed on Diamonsil C 18 column with mobile phase consisted of 0.1% formic acid solution-acetonitrile (gradient elution )at the flow rate of 1.0 mL/min. The column temperature was 30 ℃,and detection wavelength was 238 nm. The sample size was 10 μL. Using chlorogenic acid as reference,HPLC fingerprint of 10 batches of L. japonica from different production areas was established according to TCM Chromatographic Fingerprint Similarity Evaluation System (2012 edition). By comparing with reference substance ,chemical constituents corresponding to common peaks were identified ,and the similarity analysis was conducted. Acute and chronic inflammatory models of mice induced by xylene ,carrageenan and cotton ball were used to evaluate inhibition rate of 10 batches of L. japonica to ear,foot and granuloma swelling; the average value was calculated as the comprehensive pharmacodynamic index. The spectrum-effect relationship with HPLC fingerprint of L. japonica and anti-inflammatory effect was analyzed by grey relational analysis (GRA)and partial least squares regressiosn (PLSR)based on common peak area and comprehensive pharmacodynamic index . Chromatographic peaks with correlation>0.7 and regression coefficient of PLSR model >0 were characteristic peaks. The percentage of peak areas of characteristic peaks to peak areas of common peak was calculated in 10 batches of L. japonica (e.g.“peak ratio ”). RESULTS : There were 25 common peaks in HPLC fingerprints of 10 batches of L. japonica ,with similarity of 0.775-0.994. Totally 9 peaks were confirmed ,i.e. rutin (peak 18),hyperoside(peak 20),isochlorogenc acid B (peak 22),galuteolin(peak 21),chlorogenc acid(peak 9),loganin(peak 10),neochlorogenic acid (peak 2),isochlorogenic acid C (peak 25),isochlorogenic acid A (peak 23). All 10 batches of L. japonica had inhibitory effects on ear swelling ,foot swelling and granuloma ,with average inhibitory rate of 47.95%-56.52%. The correlation by GRA was peak 8>12>18>16>3>11>20>22>19>21>1>9>10>13>24>14>2> 17>25>23>5>4>15,and all of correlations were greater than 0.7. The regression coefficient of PLSR for peaks 2,4,5,7,8, 10,12,13,14,15,16,17,18,20,21,22,24 were all greater than 0;those peaks were positively correlated with anti-inflammatory effect and were characteristic peaks except for peak 7; among them ,VIP values of peaks 5,8,10,16,18, 20,24 were greater than 1. The peak ratio of 10 batches of L. japonica was 58.61%-71.19%. CONCLUSIONS :HPLC fingerprint of 10 batches of L. Japonica is successfully established. 10 batches of samples have similar components ,and the content of anti-inflammatory components is relatively high. The proportion of characteristic peaks to common peaks should not be less than 51.8%.

19.
Article in Chinese | WPRIM | ID: wpr-828948

ABSTRACT

OBJECTIVE@#To investigate the optimal dose range of immunosuppressants in patients with autosomal dominant polycystic kidney disease (ADPKD) after renal transplantation.@*METHODS@#A cohort of 68 patients with ADPKD who received their first renal transplantation between March, 2000 and January, 2018 in our institute were retrospectively analyzed, with 68 non-ADPKD renal transplant recipients matched for gender, age and date of transplant as the control group. We analyzed the differences in patient and renal survival rates, postoperative complications and concentrations of immunosuppressive agents between the two groups at different time points within 1 year after kidney transplantation. The concentrations of the immunosuppressants were also compared between the ADPKD patients with urinary tract infections (UTI) and those without UTI after the transplantation.@*RESULTS@#The recipients with ADPKD and the control recipients showed no significantly difference in the overall 1-, 5-, and 10- year patient survival rates (96.6% 96.0%, 94.1% 93.9%, and 90.6% 93.9%, respectively; > 0.05), 1-, 5-, and 10-year graft survival rates (95.2% 96.0%, 90.8% 87.2%, and 79.0% 82.3%, respectively; > 0.05), or the incidences of other post- transplant complications including acute rejection, gastrointestinal symptoms, cardiovascular events, pneumonia, and neoplasms ( > 0.05). The plasma concentrations of both tacrolimus and mycophenolate mofetil (MPA) in ADPKD group were significantly lower than those in the control group at 9 months after operation ( < 0.05). The incidence of UTI was significantly higher in ADPKD patients than in the control group ( < 0.05). In patients with ADPKD, those with UTI after transplantation had a significantly higher MPA plasma concentration ( < 0.05).@*CONCLUSIONS@#In patients with ADPKD after renal transplant, a higher dose of MPA is associated with a increased risk of UTI, and their plasma concentrations of immunosuppressants for long-term maintenance of immunosuppression regimen can be lower than those in other kidney transplantation recipients.


Subject(s)
Graft Survival , Humans , Immunosuppressive Agents , Kidney Transplantation , Polycystic Kidney, Autosomal Dominant , Retrospective Studies
20.
Article in Chinese | WPRIM | ID: wpr-828630

ABSTRACT

OBJECTIVE@#To study the clinical screening and genetic diagnosis of children suspected of Prader-Willi syndrome (PWS), as well as the differences in the scores of clinical diagnostic criteria among the children with a confirmed diagnosis of PWS.@*METHODS@#A total of 94 children suspected of PWS who were admitted from July 2016 to December 2018 were enrolled as subjects. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) was performed to confirm the diagnosis. For the children with a confirmed diagnosis of PWS, the scores of clinical diagnostic criteria were determined, and the perinatal characteristics were analyzed.@*RESULTS@#A total of 11 children with PWS were confirmed by MS-MLPA, with a detection rate of 12%, among whom there were 7 boys and 4 girls, with a median age of 3 years and 4 months (range 25 days to 6 years and 8 months) at the time of confirmed diagnosis. Among the 11 children with PWS, only 5 children (45%) met the criteria for clinical diagnosis. The main perinatal characteristics of the children with PWS were decreased fetal movement (9 cases, 82%), cesarean section birth (11 cases, 100%), hypotonia (11 cases, 100%), feeding difficulties (11 cases, 100%), and weak crying (11 cases, 100%).@*CONCLUSIONS@#Gene testing should be performed as early as possible for children suspected of PWS by clinical screening. PWS may be missed if only based on the scores of clinical diagnostic criteria.


Subject(s)
Cesarean Section , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Methylation , Muscle Hypotonia , Prader-Willi Syndrome , Pregnancy
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