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1.
Chinese Journal of School Health ; (12): 417-420, 2022.
Article in Chinese | WPRIM | ID: wpr-923141

ABSTRACT

Objective@#To understand the current situation of psychological and behavioral development of Tibetan children aged 0-5 years in Tibet, and to provide basic data for analyzing the relationship between stunning and children s psychological and behavioral development.@*Methods@#A total of 2 788 Tibetan children aged 0-71 months were randomly selected from seven districts and counties in Tibet from September to November 2020 by stratified PPS method and cluster sampling method for questionnaire survey and physical examination. Multivariate Logistic model was used to analyze the effect of stunting on psychological and behavioral development.@*Results@#The detection rate of psychological and behavioral development deviation of Tibetan children aged 0-5 years in Tibet was 24.68%, among which the detection rates of development deviation in language, gross motor skill, fine motor skill, and social emotional were 8.07%, 9.15%, 12.02% and 12.73%, respectively. After adjusting for potential mixed factors, the OR value (95% CI ) of the risk of psychological and behavioral development deviation of stunted children was 1.34(1.05-1.70). There was positive association with the three dimensions of language, fine motor skill, and social [ OR value (95% CI ) was 1.44(1.01-2.05 ), 1.50(1.06-2.12) and 1.54(1.14-2.07)], respectively. Subgroup analysis showed significant differences in age (0-35 months old: OR =1.16, 95% CI =0.88-1.53; 36-71 months old: OR =2.28, 95% CI =1.44-3.62) and gender (boys: OR =1.69, 95% CI =1.23-2.32; girls: OR =0.98, 95% CI =0.67-1.42) in the association between stunting and psychological and behavioral development.@*Conclusion@#The detection rate of psychological and behavioral development deviation of 0-5 year old Tibetan children is high. Stunting is associated with the risk of psychological and behavioral development. Early screening and comprehensive intervention of children s nutritional and neurodevelopmental should be carried out, especially among older age boys.

2.
Article in English | WPRIM | ID: wpr-922091

ABSTRACT

Development of thoracolumbar vertebra (TLV) and rib primordium (RP) is a common evolutionary feature across vertebrates, although whole-organism analysis of the expression dynamics of TLV- and RP-related genes has been lacking. Here, we investigated the single-cell transcriptome landscape of thoracic vertebra (TV), lumbar vertebra (LV), and RP cells from a pig embryo at 27 days post-fertilization (dpf) and identified six cell types with distinct gene expression signatures. In-depth dissection of the gene expression dynamics and RNA velocity revealed a coupled process of osteogenesis and angiogenesis during TLV and RP development. Further analysis of cell type-specific and strand-specific expression uncovered the extremely high level of HOXA10 3'-UTR sequence specific to osteoblasts of LV cells, which may function as anti-HOXA10-antisense by counteracting the HOXA10-antisense effect to determine TLV transition. Thus, this work provides a valuable resource for understanding embryonic osteogenesis and angiogenesis underlying vertebrate TLV and RP development at the cell type-specific resolution, which serves as a comprehensive view on the transcriptional profile of animal embryo development.

3.
Article in Chinese | WPRIM | ID: wpr-911568

ABSTRACT

Objective:To explore superior mediastinal lymph node zoning of thyroid cancer for accurate anatomical definition as a reference for surgical access. Methods:A method for zoning superior mediastinal lymph nodes for thyroid cancer was proposed. From Sep 2018 to Nov 2019, 36 cases of thyroid cancer with superior mediastinal lymph nodes metastases were reviewed. The diagnosis, surgical approaches, pathology, characteristics of lymph node metastasis, complications and follow-up results were analyzed.Results:The superior mediastinal lymph nodes were grouped into 10 areas: 1, 2Ra, 2Rb, 2La, 2Lb, 3A, 3P, 4R, 4La and 4Lb. According to the location of the superior mediastinum metastatic lymph nodes, direct vision approach through the neck incision, laparoscope-assisted approach, thracoscepy, laparoscope-assisted combined with thrascopy approach or conventional median sternotomy was performed for regional lymph node dissection. The average follow-up time was (10±4) months. No residual or recurrence of tumor in superior mediastinal area was found.Conclusions:The zoning of the superior mediastinal lymph nodes in thyroid cancer can be used as a guide for surgical approach to lymph node dissection .

4.
Chinese Critical Care Medicine ; (12): 131-138, 2021.
Article in Chinese | WPRIM | ID: wpr-883845

ABSTRACT

The epidemic of coronavirus disease 2019 (COVID-19) puts higher demands on critical care medicine. Lots of studies have been conducted to solve COVID-19-related problems. Therefore, we reviewed the annual progress for COVID-19-related issues including antivirals threapies, respiratory support and immunomodulatory therapies and other critical issues, including the effect of antibiotic on mitochondrial damage and its relationship with sepsis, the goal and direction of antimicrobial de-escalation, drug prophylaxis of constipation, bleeding in gastrointestinal disorders and management of critical illness in the informalization era and so on. We hope to provide reference for clinical and scientific research work of the intensivists.

5.
Journal of Chinese Physician ; (12): 1429-1433, 2021.
Article in Chinese | WPRIM | ID: wpr-909717

ABSTRACT

In the past, it was thought that insulin autoantibodies (IAA) produced by exogenous insulin and its analogues rarely had clinical significance. However, in recent years, with the increase in the incidence of diabetes and the use of insulin, more and more exogenous IAA produced by insulin causes serious clinical harm, and clear detection methods and treatment methods are limited. A large proportion of such patients are therefore missed, which seriously affects the blood glucose control and quality of life of patients with type 2 diabetes who receive insulin therapy. This article reviews the current diagnosis and treatment progress of exogenous insulin antibody syndrome in patients with type 2 diabetes.

6.
Chinese Journal of Nephrology ; (12): 519-525, 2020.
Article in Chinese | WPRIM | ID: wpr-870986

ABSTRACT

Objective:To assess the bacterial profiles and antimicrobial susceptibility patterns in uropathogens, and help to inform the empiric treatment decisions for urinary tract infection in outpatient settings.Methods:A single institutional retrospective analysis was performed on positive urine cultures from outpatient settings between January 1998 and December 2018. To analyze changes over time, trends analysis were undertaken on bacterial profiles, antimicrobial susceptibility and resistance.Results:A total of 1.172 pathogenic bacteria were isolated after exclusion of duplicate strains originated from the same patient, including 991(84.6%) Gram-negative bacterial strains and 181(15.4%) Gram-positive strains. The most common Gram-negative uropathogens were Escherichia coli (60.8%) and Klebsiella pneumonia (8.1%). Enterococcus faecalis (4.6%) was the predominant Gram-positive strain. The detection rate of Escherichia coli increased significantly, from 50.8% to 63.2% ( χ2=7.978, P=0.046), and no significant difference was observed in the distribution of major uropathogenic bacteria over the 20 years (all P>0.05). The proportion of extended-spectrum β-lactamase (ESBLs) producing strains increased significantly across the 20 years ( P<0.05). The resistance rates of Escherichia coli to amoxicillin and clavulanate potassium, aztreonam, ceftazidime, ciprofloxacin and sulbactam + cefoperazone increased significantly (all P<0.05). All the isolates sustained high susceptibility to tazobactam + piperacillin, amikacin, imipenem and nitrofurantoin (95.0%, 95.7%, 97.9% and 91.1%). Similar to those of Escherichia coli, Klebsiella pneumoniae remained a high and stable sensitivity to tazobactam+piperacillin, amikacin and imipenem during the 20 years (79.1%, 88.0% and 80.3%). However, the proportion of ESBLs producing strains increased significantly ( P<0.05). Among Gram-positive bacteria isolates, the sensitivity rates of Enterococcus faecalis to ampicillin, nitrofurantoin and penicillin G were 100.0%. No vancomycin resistant strain was detected in Gram-positive bacteria. Conclusions:From 1998 to 2018, Escherichia coli and Klebsiella pneumoniae are the most common Gram-negative bacteria uropathogens obtained in outpatient settings. Significant increases of resistance to some antimicrobial agents such as second- and third-generation cephalosporins and fluoroquinolones are observed during the 20 years and high susceptibilities to tazobactam+piperacillin, amikacin, imipenem and nitrofurantoin sustain over time. Local treatment strategies of urinary tract infections on outpatient basis should be made according to epidemiology of drug resistance and individual characteristics to control the spread and curb the prevalence of drug resistant.

7.
Journal of Clinical Hepatology ; (12): 775-777, 2020.
Article in Chinese | WPRIM | ID: wpr-819186

ABSTRACT

The epidemic of coronavirus disease 2019 (COVID-19) has become a severe and complicated situation. As of February 23, 2020, there have been more than 77,038 confirmed cases of new coronavirus infection nationwide. COVID-19 is highly infectious and has a long incubation period and a variety of clinical manifestations, which has a great impact on society and economy and also seriously affects the daily operation of hepatobiliary surgery. This article discusses and recommends the medical protection measures required for outpatient, ward, and operation of hepatobiliary surgery, in order to reduce the risk of nosocomial infection in hepatobiliary surgery during the COVID-19 epidemic.

8.
Article in Chinese | WPRIM | ID: wpr-805376

ABSTRACT

Objective@#To study infection of coxsackievirus A6 (CV-A6) in Mongolian gerbils.@*Methods@#To screen the optimal ages of Mongolian gerbils, five groups with different ages were infected with 1×105 TCID50 dose of CV-A6 XS45 strain by intraperitoneal, and symptom scores of Mongolian gerbils were collected. Then to estimate the dose-effect, three doses of virus were injected to the Mongolian gerbils. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry(IHC) were used to determine virus load and tissues infection in muscle, brain and intestinal tract.@*Results@#Mongolian gerbils infected with 1×105 TCID50 dose CV-A6 consistently exhibited clinical signs, and the morbidity (death) rates of five age groups were up to 100%. There was a positive correlation between the trend of symptom scores changes and ages. The morbidity (death) rates of three doses (1×103 TCID50, 1×104 TCID50, 1×105 TCID50) also were up to 100% in 28 days Mongolian gerbils. The correlation between the trend of symptom scores changes and doses were negative. Virus loads were detected in muscle, brain and intestinal tract of pathogenesis animal. The virus loads of muscle were higher than others. IHC results showed virus infection and cytopathic effects in three tissues.@*Conclusions@#Mongolian gerbils had high susceptibility to CV-A6, and were best for animal model of CV-A6 infection.

9.
Chinese Journal of Nephrology ; (12): 481-487, 2018.
Article in Chinese | WPRIM | ID: wpr-711129

ABSTRACT

Objective To investigate the clinico-pathological features and renal outcomes of primary IgA nephropathy (IgAN) with glomerular IgM deposition.Methods Primary IgAN diagnosed with biopsy from January 2006 to December 2011 were recruited.Patients were divided into groups according to IgM deposition (Group A) and without IgM deposition (Group B).In addition,Group A was subdivided into two groups based on the position of IgM deposits as the mesangium (Group A1) and both mesangium and capillary wall (Group A2).Renal outcomes were defined as end stage renal disease (ESRD) and/or the doubling of baseline serum creatinine.Clinico-pathological features were retrospectively compared.Kaplan-Meier was conducted for renal outcomes,and Cox regression model was used to analyze the prognostic value of IgM deposition and the position of IgM deposition in the progression of nephropathy in IgAN patients.Results 939 patients were enrolled with 422 (44.9%) having IgM deposition (Group A).Of the 422 patients,382 patients were divided as Group A 1,whereas 40 patients were noted as Group A2.Compared to Group B,hemoglobin,serum protein,albumin and serum IgG levels in group A were significantly lower,and the cholesterol and serum IgM levels were significantly higher (all P < 0.05).There was no significant difference in serum creatinine,estimated glomerular filtration rate (eGFR),urinary protein,blood pressure and uric acid between group A and B.In terms of pathological manifestations,patients in Group A exhibited more severe histological lesions including glomerular sclerosis,S1,M1 and interstitial inflammatory cell infiltration (all P<0.05).Immunofluorescence showed that the proportion of IgG,C1q and Fg deposition in group A was significantly higher than that in group B (all P < 0.05).By Kaplan-Meier,cumulative renal survival rate has no significant difference between Group A and B (Log-rank test x2=0.019,P=0.891).Univariate and muhivariable Cox regression analysis showed that IgM deposition had no significant effect on the renal progression in IgAN patients.Subgroup analysis showed that patients in Group A2 exhibited higher urine protein,creatinine and blood pressure,and lower eGFR and serum albumin,also had worse histological lesions including M1,E1 and T1-2 of Oxford classification (all P<0.05),Immunofluorescencc showed that the proportion of IgG,C1q and Fg deposition in group A2 was significantly higher than that in group A1 (all P < 0.05).By Kaplan-Meier,renal survival rates calculated from outcomes were lower in Group A2 (Log-rank test x2=1 8.207,P < 0.001).In addition,IgM deposited both in the mesangium and capillary wall was a risk factor for renal progression of IgAN patients with IgM deposition by a univariate Cox hazards regression mode and multivariable-adjusted Cox models (HR=3.621,95%CI 1.924-6.814,P< 0.001;HR=2.309,95%CI 1.176-4.533,P=0.015respectively).Conclusions The IgAN patients with IgM deposition relatively had more severe clinicopathological changes,especially those with IgM deposited both in the mesangium and capillary wall.In this study,IgM deposition was not found to be an independent risk factor for the prognosis of kidney in IgAN patients.However,IgM deposited both in the mesangium and capillary wall was an independent risk factor for renal prognosis in IgAN patients with IgM deposition.

10.
China Pharmacist ; (12): 112-114, 2018.
Article in Chinese | WPRIM | ID: wpr-705464

ABSTRACT

Objective:To observe the clinical effect of Xuefuzhuyu decoction in the treatment of cancerous fever .Methods:Ac-cording to the admission order , 62 cases of patients with cancerous fever were divided into observation group (32 cases) and control group(30 cases).The observation group was treated with Xuefuzhuyu decoction , while the control group was treated with naproxen . The treatment course was 7 days.The changes of body temperature and KPS score were compared between the groups before and after the treatment,and the clinical efficacy of the two groups were evaluated .Results:After the 5-7-day treatment, the average body tem-perature in the observation group was significantly lower than that in the control group (P<0.05).After the treatment, the total effec-tive rate of the observation group was 75%, which was much higher than that (50%) of the control group (P<0.05).The rate of KPS score keeping stable or ≥10 in the observation group was much higher than that in the control group (P<0.05).Conclusion:Xuefuzhuyu decoction is effective and safe in the treatment of cancerous fever with better efficacy than naproxen , which can improve pa-tients' quality of life with important clinical significance .

11.
China Oncology ; (12): 353-358, 2017.
Article in Chinese | WPRIM | ID: wpr-618815

ABSTRACT

Background and purpose: Short tandem repeats (STR) multiplex PCR fluorescence detection technology is the most widely used DNA technology in individual identity and genetic identification. It's the most direct method to obtain accurate conclusions. However, some studies have indicated that the rate of STR mutations in tumor tissue is significantly higher than that in normal tissues or blood. This study aimed to investigate the tendency of genetic instability in 20 STR loci on autosomal and Amel loci in tumor tissue samples from lung cancer. Methods: This study, collected 75 cases of human lung cancer tissues and the adjacent normal tissues. DNA samples were extracted by tissue DNA extraction kit, amplified using MicroreaderTM 21 Direct ID System PCR amplification kit. Capillary electrophoresis was performed using API 3130 analyzer, and results were analyzed by genetic analysis software (Gene Mapper ID V3.2). Results: STR alterations were detected in 24 specimens from 75 lung cancer tissues (32%). Fifty-five alterations were detected in the frequently used 21 STR loci in total, including additional alleles 10 times, loss of heterozygosity 10 times, partial loss of heterozygosity 35 times. Partial loss of heterozygosity was the most common genetic alteration types accounting for 63.64% of the total alteration frequency. And multiple genetic alteration types could occur in the same lung cancer tissue. Among them, the highest alteration frequency occurred on D5S818 (7 times), secondly on D3S1358 and D12S391 (both 5 times), and no alterations on D2S441 and Penta E. Combining the experimental results and analysis on clinical data, this study found the statistical differences between the staging of lung cancer and the age of the patients with the STR loci alterations (P0.05). Conclusion: STR loci of the lung cancer tissue were not stable, and the alteration occurred in the aged or high malignant degree lung cancer tissue more frequently. Meanwhile, no alteration was detected on D2S441 and Penta E. In the future research the two STR loci should be verified to determine whether they can be used as the stable STR loci in such cases by increasing the sample size.

12.
Article in Chinese | WPRIM | ID: wpr-618701

ABSTRACT

Objective To study the clinical course and clinicopathological features of para-aortic lymph node metastases in patients with gallbladder cancer.Methods Forty-two patients with gallbladder cancer who underwent radical resection combined with para-aortic lymphadenectomy at the Mianyang Hospital of Traditional Chinese Medicine from January 2001 to December 2013 were retrospectively studied.The survival rates of the para-aortic lymph node metastasis group were compared with the negative para-aortic lymph node group of patients.Para-aortic lymph node metastasis as well as clinical features were correlated with survival.Results No one died within the perioperative period.The total complication rate was 24.0%,and there was no significant difference between the positive para-aortic lymph node group and the negative group (P >0.05).The rate of para-aortic lymph node metastasis on histopathology was 21.4% (9/42),which was positively correlated with tumor depth of invasion and negatively correlated with the degree of differentiation (P < 0.01).The 1-,2-,and 3-year survival rates of the positive para-aortic lymph node group were significantly inferior to the negative group (P < 0.05).Conclusions Dissection of para-aortic lymph nodes in patients with gallbladder cancer was safe and feasible.Lymphadenectomy did not improve the longterm survival rates of patients with para-aortic lymph node involvement metastases.The extent of lymph node dissection for gallbladder cancer should be decided by intraoperative biopsy.

13.
Chongqing Medicine ; (36): 4633-4635,4638, 2017.
Article in Chinese | WPRIM | ID: wpr-668540

ABSTRACT

Objective To analyze the current status of bacterial spectrum and drug resistance in community-acquired biliary tract infection to provide a basis for clinical medication .Methods The patients with community-acquired biliary tract infection (ex-periment group) and the patients with biliary tract diseases without biliary tract infection (control group) derived from the native ar-ea treated in this hospital from September 2014 to January 2016 were selected .The bile juice was intraoperatively extracted for con-ducting the bacterial culture and drug susceptibility test .Results Thirteen specieses (60 strains) of bacteria were isolated in the ex-periment group .The top 3 specieses were Escherichia coli (35 .0% ) ,Klebsiella pneumonia (21 .7% ) and Enterobacter cloacae (10 .0% ) .Eight specieses (13 strains) of bacteria were isolated in the control group .The top 3 specieses were Escherichia coli (30 .8% ) ,Klebsiella pneumonia(15 .4% ) and Lactococcus garvieae (15 .4% ) .The proportions of drug resistant strains in the two groups were 95 .0% and 84 .6% respectively (P>0 .05) .The proportions of multiple drug resistant strains in the two groups were 30 .0% and 7 .7% respectively(P>0 .05) .The occurrence rates of multiple drug resistance in the top 3 specieses of bacteria in the experiment group were 61 .9% ,7 .7% and 16 .7% respectively .Conclusion The bacterial spectra of community-acquired acute bili-ary tract infection in the native area are dominated by Gram negative bacteria .The total bacterial drug resistance is serious ,but the drug resistance situation in different bacteria pathogens is different .

14.
Chongqing Medicine ; (36): 3912-3914, 2017.
Article in Chinese | WPRIM | ID: wpr-661482

ABSTRACT

Objective To investigate the autopsy clinicopathological characteristics of pulmonary thromboembolism(PTE)and to improve the understanding of PTE.Methods The clinicopathological data of 47 death cases of PTE verified by autopsy in the Chongqing Medical University during 2004? 2015 were retrospectively analyzed.Results A total of 784 cases of autopsy were completed during these 12 years,of which 47 cases(6.0%) were proved as PTE (2 cases complicating fat embolism) and 46 cases (97.9%) were fatal pulmonary embolism (FPE).The analysis on 47 cases of autopsy data found that each case had at least 1 risk factor and 43 cases (91.3%) had two or more risk factors,and the most common risk factors were traumatic fracture(55.3%) and surgery (53.2 %).The frequent clinical symptoms were dyspnea (40.4 %),cardiac arrest (31.9 %) and syncope(27.7 %);24 cases (51.1%) were complicated with deep venous thrombus (DVT) of lower limbs,8 cases (17.0%) of complicating right cardiac thrombus and 1ease (2.1%) of basilar artery embolism were found.The embolism location was dominated by main pulmonary artery (59.6 %),including 20 cases (42.6 %) of straddling type pulmonary embolism.Twenty-two cases (46.8 %) were suspiciously diagnosed as PTE before death,but none was given anticoagulation or thrombolytic therapy.Only 1 case had low molecular heparin to prevent thrombosis.Conclusion PTE is more likely to occur in those patients with high risk factors,especially traumatic fractures and surgeries.Its clinical symptoms have no specificity,lack triad syndrome,has higher misdiagnosis rate,missed diagnosis rate and fatality rate.The treatment on suspected PTE patients by clinicians is insufficient.

15.
Chongqing Medicine ; (36): 3912-3914, 2017.
Article in Chinese | WPRIM | ID: wpr-658563

ABSTRACT

Objective To investigate the autopsy clinicopathological characteristics of pulmonary thromboembolism(PTE)and to improve the understanding of PTE.Methods The clinicopathological data of 47 death cases of PTE verified by autopsy in the Chongqing Medical University during 2004? 2015 were retrospectively analyzed.Results A total of 784 cases of autopsy were completed during these 12 years,of which 47 cases(6.0%) were proved as PTE (2 cases complicating fat embolism) and 46 cases (97.9%) were fatal pulmonary embolism (FPE).The analysis on 47 cases of autopsy data found that each case had at least 1 risk factor and 43 cases (91.3%) had two or more risk factors,and the most common risk factors were traumatic fracture(55.3%) and surgery (53.2 %).The frequent clinical symptoms were dyspnea (40.4 %),cardiac arrest (31.9 %) and syncope(27.7 %);24 cases (51.1%) were complicated with deep venous thrombus (DVT) of lower limbs,8 cases (17.0%) of complicating right cardiac thrombus and 1ease (2.1%) of basilar artery embolism were found.The embolism location was dominated by main pulmonary artery (59.6 %),including 20 cases (42.6 %) of straddling type pulmonary embolism.Twenty-two cases (46.8 %) were suspiciously diagnosed as PTE before death,but none was given anticoagulation or thrombolytic therapy.Only 1 case had low molecular heparin to prevent thrombosis.Conclusion PTE is more likely to occur in those patients with high risk factors,especially traumatic fractures and surgeries.Its clinical symptoms have no specificity,lack triad syndrome,has higher misdiagnosis rate,missed diagnosis rate and fatality rate.The treatment on suspected PTE patients by clinicians is insufficient.

16.
Chinese Journal of Nephrology ; (12): 881-886, 2017.
Article in Chinese | WPRIM | ID: wpr-711071

ABSTRACT

Objective To explore the clinicopathological features and renal outcomes of primary IgA nephropathy (IgAN) patients with chronic tonsillitis.Methods Patients with biopsyproven primary IgAN admitted to The First Affiliated Hospital,Sun Yat-sen University from January 2006 to December 2011 were enrolled.The clinicopathological features and renal outcomes of patients with and without chronic tonsillitis were retrospectively compared.The primary outcome was progression to end stage renal diseases and/or doubling of serum creatinine.Results A total of 981 primary IgAN patients were enrolled and 98 patients (9.99%) had a history of chronic tonsillitis.Compared with patients without chronic tonsillitis,IgAN patients with chronic tonsillitis exhibited significantly higher prevalence of acute episodes of tonsillitis as a predisposition (P < 0.001),higher serum IgA levels (P=0.012),and higher prevalence of macrohematuria (P=0.006).No significant difference in renal pathological features was observed in patients with and without chronic tonsillitis.Moreover,the renal outcomes were similar as regards IgAN patients with and without chronic tonsillitis.Conclusion IgAN patients with chronic tonsillitis had higher prevalence of acute episodes of tonsillitis and macrohematuria as well as higher serum IgA levels.However,IgAN patients with and without chronic tonsillitis showed no significant difference in renal pathological features and renal outcomes.

17.
Military Medical Sciences ; (12): 260-264,286, 2017.
Article in Chinese | WPRIM | ID: wpr-621514

ABSTRACT

Objective To investigate the protective and therapeutic effect of chalcone ketones compound(code:L2H17,hereinafter referred to as L2)on mice infected with influenza A virus.Methods BALB/c mice were randomly divided into six groups:normal group,model group,positive drug-treated group,L2 treated groups (3 different concentrations).The mice were adapted for 72 hours,before a model was established by intranasal infection.Mice in each group were given medicine by i.g once daily for 6 days starting 24 h before virus challenge.Survival was observed daily for 14 days.The mortality,median survival time,rate of death protection and rate of prolonging life were determined to observe the therapeutic effect of chalcone(L2) against influenza virus infection.The whole lungs were taken under aseptic conditions on days 3 and 5 post-infection to calculat lung indexes and lung index inhibition.The left lung was fixed with 4% formaldehyde for pathological biopsy,the right lung was soaked in RNAstore to detect lung tissue viral load,and the double antibody sandwich ELISA method was used to detect the expression of inflammatory cytokines IL-6 and TNF-α in order to observe the therapeutic effect of L2 on viral pneumonia caused by influenza virus infection.Results Compared with the model group,the L2 80 mg/kg treatment group exhibited significant increases in median survival time(11 d),the rate of death protection (50%),and the rate of prolonging life(24.1%)but a moderate 50% decrease in mortality.In addition,the lung index decreased significantly both on d 3 and 5 after virus infection (P<0.05).The pathological results also improved significantly.The L2 80 mg/kg dose group had a significantly lower viral load of lung tissue on d 3 and 5 post-infection(P<0.05).Compared with model group,the expression of inflammatory factor IL-6 became lower to different degrees.Conclusion L2 has a protective effect on mice infected with influenza virus by reducing the degree of pathological changes of pneumonia caused by influenza viruses.

18.
Recent Advances in Ophthalmology ; (6): 527-530,547, 2017.
Article in Chinese | WPRIM | ID: wpr-620125

ABSTRACT

Objective To investigate the protective effects of piceatannol on retinal ganglion cells (RGCs) in rats with glaucoma.Methods Forty rats were randomly divided into 4 groups:control group,model group,low dose piceatannol treatment group and high dose piceatannol treatment group.Photocoagulation method was used to establish the experimental glaucoma model in rats,and then rats were given 100 mg· kg-1 or 200 mg · kg-1 of piceatannol by gavage.The intraocular pressure was measured before and after the model was established.Rat retinal ganglion cells were labeled and counted using FG staining.Retinal tissue pathological morphology was observed by HE staining.The protein expression of p-JNK,p-c-Jun,p-ERK,p-p38 MAPK and TNF-α were measured by western blot.Results Compared with control group,the intraocular pressure,the protein expressions of p-JNK,p-c-Jun p-ERK,p-p38 MAPK and TNF-α were significantly increased in model group (all P < 0.05).However,the number of RGCs were lower in model group(P <0.05).Furthermore,there were cavitation and edema changes in retinal tissue of model group.Compared with model group,piceatannol treatment markediy increased the number of RGCs(P =0.003,0.002),improved the pathological morphology of retinal tissue,and reduced the protein expressions of p-JNK,p-c-Jun p-ERK,p-p38 MAPK and TNF-α (all P < 0.05),especially for the high concentration.Conclusion Piceatannol can protect against RGCs injury in glaucoma rats,and the mechanism may be associated with inhibition of MARK signaling pathway.

19.
Int. braz. j. urol ; 42(3): 546-549, graf
Article in English | LILACS | ID: lil-785718

ABSTRACT

ABSTRACT Objectives To determine whether spot urine pH measured by dipstick is an accurate representation of 24 hours urine pH measured by an electrode. Materials and Methods We retrospectively reviewed urine pH results of patients who presented to the urology stone clinic. For each patient we recorded the most recent pH result measured by dipstick from a spot urine sample that preceded the result of a 24-hour urine pH measured by the use of a pH electrode. Patients were excluded if there was a change in medications or dietary recommendations or if the two samples were more than 4 months apart. A difference of more than 0.5 pH was considered an inaccurate result. Results A total 600 patients were retrospectively reviewed for the pH results. The mean difference in pH between spot urine value and the 24 hours collection values was 0.52±0.45 pH. Higher pH was associated with lower accuracy (p<0.001). The accuracy of spot urine samples to predict 24-hour pH values of <5.5 was 68.9%, 68.2% for 5.5 to 6.5 and 35% for >6.5. Samples taken more than 75 days apart had only 49% the accuracy of more recent samples (p<0.002). The overall accuracy is lower than 80% (p<0.001). Influence of diurnal variation was not significant (p=0.588). Conclusions Spot urine pH by dipstick is not an accurate method for evaluation of the patients with urolithiasis. Patients with alkaline urine are more prone to error with reliance on spot urine pH.


Subject(s)
Humans , Urine/chemistry , Urinalysis/methods , Urolithiasis/urine , Hydrogen-Ion Concentration , Reference Standards , Reference Values , Time Factors , Logistic Models , Predictive Value of Tests , Reproducibility of Results , Retrospective Studies , Urinalysis/instrumentation , Electrodes
20.
Chinese Journal of Pathophysiology ; (12): 1323-1326, 2016.
Article in Chinese | WPRIM | ID: wpr-496545

ABSTRACT

[ ABSTRACT] AIM:To investigate the effect of Ginkgo biloba extract ( GBE) on diabetic retinopathy ( DR) and its possible mechanism in rats .METHODS:Goto-Kakizaki ( GK) rats were used as a DR model, and were treated with different doses of GBE.Normal Wistar rats were used as the control.Blood glucose and retina barrier injury were analyzed, respectively.Ganglion cell apoptosis was detected by TUNEL staining.Moreover, the protein expression of nuclear factor E2-related factor 2( Nrf2) , ERK, Bcl-2 and P53, and ERK phosphorylation were examined by Western blot.RESULTS:GBE reduced blood glucose in the DR rats, attenuated retina barrier injury, and decreased the apoptosis of ganglion cells. Furthermore, the expression of Nrf2 and Bcl-2, and phosphorylation of ERK were increased after GBE treatment, whereas P53 expression was decreased.CONCLUSION:GBE protects ganglion cells against apoptosis in DR rats, which may be through activation of Nrf2/ERK pathway and regulating Bcl-2 and P53 expression.

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