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2.
Article in Chinese | WPRIM | ID: wpr-928153

ABSTRACT

An UPLC-Q-TOF-MS method was employed to characterize and classify the chemical components of the standard decoction of Yiguanjian, a classical famous recipe. Chromatographic separation was performed on an Acquity HSS T3(2.1 mm ×100 mm, 1.8 μm) column with a mobile phase of 0.1% formic acid water-0.1% formic acid acetonitrile using gradient elution. The flow rate was 0.4 mL·min~(-1) and the column temperature was 40 ℃. Mass spectrometry was performed on electrospray ionization source(ESI) with positive and negative ion scanning modes. The potential compounds were identified by comparing the reference compounds, analyzing the mass spectrometry data and matching the published articles on Masslynx 4.1 software and SciFinder database. Finally, a total of 113 compounds, including 11 amino acids, 19 terpenoids, 13 phthalides, 11 steroidal saponins, 10 coumarins, 9 alkaloids, 7 flavonoids, 8 phenylethanoid glycosides, 8 organic acids and 17 other categories were identified. The established method systematically and accurately characterized the chemical components in Yiguanjian, which could provide experimental evidences for the subsequent studies on the pharmacodynamical material basis and quality control of Yiguanjian.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/chemistry , Flavonoids/analysis , Formates , Glycosides/analysis , Prescriptions
3.
Article in Chinese | WPRIM | ID: wpr-928057

ABSTRACT

This paper aims to study the effect of Xiangqin Jiere Granules(XQ) on lipid metabolism and chronic inflammation in different obesity model mice. The monosodium glutamate(MSG) obese mouse model was established by subcutaneous injection of MSG in newborn mice, and the high fat diet(HFD) obese mouse model was established by feeding adult mice with HFD. The normal mice were assigned into the control group; the MSG obese mice were assigned into MSG model group, XQ4.5 group(Xiangqin Jiere Granu-les, 4.5 g·kg~(-1)), XQ22.5 group(Xiangqin Jiere Granules, 22.5 g·kg~(-1)); the HFD obese mice were assigned into HFD model group, XQ4.5 group, and XQ22.5 group. The mice were intragastrically administrated with saline or XQ for 5 weeks. After that, the body weight, visceral fat mass, liver and thymus weight, and the organ indexes in each group were measured. The levels of triglyceride(TG), total cholesterol(TC), and low-density lipoprotein cholesterol(LDL-c) in serum and liver tissue were detected by the kits. The mRNA expression levels of acetyl CoA carboxylase 1(ACC1), fatty acid synthetase(FAS), diacylgycerol acyltransferase 1(DGAT1) and hepatic lipase(HTGL) involved in lipid metabolism in mouse liver tissue were detected by quantitative real-time PCR(qPCR). The protein levels of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in serum were detected by ELISA, and the mRNA levels of TNF-α and IL-6 in liver tissue were detected by qPCR. Compared with the control group, MSG and HFD mice showed increased body weight, abdominal circumference, Lee index and visceral fat mass as well as elevated levels of TG, TC, and LDL-c in serum. The model mice had up-regulated gene levels of ACC1, FAS and DGAT1 while down-regulated gene level of HTGL in the liver. Furthermore, the mRNA and protein levels of IL-6 increased in the model mice. Compared with the model mice, XQ treatment decreased the body weight, abdominal circumference, Lee index, and visceral fat mass, lowered the levels of TG, TC, and LDL-c in se-rum, down-regulated the gene levels of ACC1, FAS, and DGAT1 in liver tissue, up-regulated the gene level of HTGL, and down-regulated the mRNA and protein levels of IL-6. To sum up, XQ has good therapeutic effect on different obesity model mice. It can improve lipid metabolism and reduce fat accumulation in obese mice by regulating the enzymes involved in lipid metabolism, and alleviate obesity-related chronic low-grade inflammation.


Subject(s)
Animals , Inflammation/metabolism , Lipid Metabolism , Mice , Mice, Inbred C57BL , Mice, Obese , Obesity/genetics
4.
Chinese Medical Journal ; (24): 697-706, 2022.
Article in English | WPRIM | ID: wpr-927556

ABSTRACT

BACKGROUND@#: Breast cancer with low-positive human epidermal growth factor receptor 2 (HER2) expression has triggered further refinement of evaluation criteria for HER2 expression. We studied the clinicopathological features of early-stage breast cancer with low-positive HER2 expression in China and analyzed prognostic factors.@*METHODS@#: Clinical and pathological data and prognostic information of patients with early-stage breast cancer with low-positive HER2 expression treated by the member units of the Chinese Society of Breast Surgery and Chinese Society of Surgery of Chinese Medical Association, from January 2015 to December 2016 were collected. The prognostic factors of these patients were analyzed.@*RESULTS@#: Twenty-nine hospitals provided valid cases. From 2015 to 2016, a total of 25,096 cases of early-stage breast cancer were treated, 7642 (30.5%) of which had low-positive HER2 expression and were included in the study. After ineligible cases were excluded, 6486 patients were included in the study. The median follow-up time was 57 months (4-76 months). The disease-free survival rate was 92.1% at 5 years, and the overall survival rate was 97.4% at 5 years. At the follow-up, 506 (7.8%) cases of metastasis and 167 (2.6%) deaths were noted. Multivariate Cox regression analysis showed that tumor stage, lymphvascular invasion, and the Ki67 index were related to recurrence and metastasis (P < 0.05). The recurrence risk prediction model was established using a machine learning model and showed that the area under the receiving operator characteristic curve was 0.815 (95% confidence interval: 0.750-0.880).@*CONCLUSIONS@#: Early-stage breast cancer patients with low-positive HER2 expression account for 30.5% of all patients. Tumor stage, lymphvascular invasion, and the Ki67 index are factors affecting prognosis. The recurrence prediction model for breast cancer with low-positive HER2 expression based on a machine learning model had a good clinical reference value for predicting the recurrence risk at 5 years.@*TRIAL REGISTRATION@#: ChiCTR.org.cn, ChiCTR2100046766.


Subject(s)
Breast Neoplasms/metabolism , Female , Humans , Ki-67 Antigen , Mastectomy , Receptor, ErbB-2/metabolism
5.
Article in Chinese | WPRIM | ID: wpr-927350

ABSTRACT

OBJECTIVE@#To observe the effect of conventional ovulation induction protocol and acupuncture combined with conventional ovulation induction protocol on pregnancy outcomes of frozen embryo transfer (FET) in patients with anovulatory infertility.@*METHODS@#A total of 60 patients with anovulatory infertility were randomized into an observation group and a control group, 30 cases in each group. In the control group, conventional ovulation induction protocol was applied to prepare endometrium. On the basis of the control group, acupuncture was started on the 2nd day of menstrual cycle in the observation group,Baihui (GV 20), Mingmen (GV 4), Geshu (BL 17), Guanyuan (CV 4), Qihai (CV 6), etc. were selected, once every other day, until 1 day before transplantation. The clinical pregnancy rate, embryo implantation rate, endometrial morphology on HCG trigger day, ovulation rate and cycle cancellation rate were compared in the two groups. The endometrial thickness before treatment and on HCG trigger day, TCM symptom score before and after treatment were observed in the two groups.@*RESULTS@#In the observation group, the embryo implantation rate and clinical pregnancy rate were higher than the control group (P<0.05), endometrial thickness and endometrial morphology on HCG trigger day were superior to the control group (P<0.05). After treatment, the TCM symptom score in the observation group was decreased compared with before treatment (P<0.05), and the variation was greater than the control group (P<0.01).@*CONCLUSION@#On the basis of the conventional ovulation induction protocol, acupuncture could enhance the embryo implantation rate and clinical pregnancy rate of FET, improve the endometrial receptivity of patients with anovulatory infertility.


Subject(s)
Acupuncture Therapy , Embryo Transfer , Female , Humans , Infertility, Female/therapy , Pregnancy , Pregnancy Outcome , Pregnancy Rate
6.
Article in Chinese | WPRIM | ID: wpr-908846

ABSTRACT

Objective:To explore the application effect of mind mapping in the experimental teaching of complete denture for the undergraduates of stomatological technology.Methods:In this study, the undergraduates of stomatological technology in Chongqing Medical University were divided into three groups. They were taught with traditional experimental teaching of complete denture, mind mapping teaching and modified mind mapping teaching respectively. The students' mastery degree were evaluated by experiment examination, theoretical examination and comprehensive examination (a weight to experiment and theoretical examination). SPSS 22.0 was used for statistical analysis.Results:The results of one-way ANOVA showed that there were significant differences in experimental examination ( F=99.471, P<0.001) and comprehensive examination ( F=22.909, P<0.001). Dunnett T3 analysis found that the comprehensive examination score of mind mapping group and modified mind mapping group were significantly improved than that of the traditional experimental group ( P<0.001). Conclusion:For the undergraduates who lack of clinical experience, mind mapping can effectively improve students' understanding and mastery of relevant knowledge in complete denture making.

7.
International Eye Science ; (12): 540-544, 2021.
Article in Chinese | WPRIM | ID: wpr-873466

ABSTRACT

@#AIM: To study the characteristics and related influencing factors of subfoveal choroidal thickness(SFCT)changing with time in children with myopia. <p>METHODS: Totally 60 eyes of 60 children with myopia aged 6-15 years old who were admitted to the Eye Hospital of Nanchang University from July 2018 to September 2018 were included. IOP, axial length, equivalent spherical degree and SFCT were measured at baseline and 6mo of follow-up, respectively. Analyze the changes of the above-mentioned eye parameters and related influencing factors of SFCT. <p>RESULTS: Compared with baseline, follow-up for 6mo, myopic children's SFCT decreased by 8±14μm(<i>P</i><0.001), the axial length increased by 0.15±0.16mm(<i>P</i><0.001), and the equivalent spherical degree increased by -0.34±0.33D(<i>P</i><0.001), and there is a significant correlation between the SFCT change value and the axial length and equivalent spherical power change value(<i>r</i>s=-0.701, 0.633, both <i>P</i><0.001), indicating faster axial growth and myopia increasing is related to more thinning of the membrane thickness. Multiple linear regression analysis shows that SFCT is significantly correlated with axial length(<i>P</i>=0.001).<p>CONCLUSION: The SFCT of myopic children becomes thinner over time, and the axial length is negatively correlated with SFCT, and children with faster axial growing and greater myopia increasing are usually accompanied by more SFCT thinning.

8.
International Eye Science ; (12): 549-552, 2021.
Article in Chinese | WPRIM | ID: wpr-873465

ABSTRACT

@#AIM: To investigate the vision-related quality of life after corneal refraction surgery of the new recruits in a navy unit. <p>METHODS:New recruits in a navy unit during 2014-2016 were interviewed druing recheck ophthalmic examination through a questionnaire, including basic data and the Visual Function Questionnaire-25(VFQ), and also checking the visual acuity, contrast sensitivity(CS), optometry.<p>RESULTS: Among 2 760 new recruits, 340 persons(12.32%)had undergone corneal refractive surgeries, which included corneal stroma surgery(89.71%)and corneal surface surgery(10.29%). According to the preoperative myopia spherical equivalent, the subjects were divided into three groups: mild myopia group(≤-3.00D, 134 cases), moderate myopia group(>-3.00 D to < -6.00D, 159 cases), and high myopia group(≥-6.00D, 47 cases). The postoperation spherical equivalent range ±1.0D is 128 cases(95.52%), 153 cases(96.23%), 38 cases(80.85%)in mild myopia group, moderate myopia group and high myopia group respectively. The contrast sensitivity(CS)were statistically significant among three different diopter groups under natural light and glare conditions(<i>P</i><0.05), the CS of the low myopia group and moderate myopia group were higher than in high myopia group(<i>P</i><0.05). The visual-related quality of life scores were statistically significant among three group(<i>P</i><0.05). The scores of low(6.25±8.61)points and moderate myopia groups(6.87±8.51)points were significantly better than that of high myopia group(9.84±9.76)points(<i>P</i><0.05).<p>CONCLUSION:The vision-related quality of life in new recruits after corneal refractive surgery in the unit is related with preoperative diopter of refractive error. The vision-related quality of life in recruit with lower diopter are better than in recruit with higher dioper.

9.
International Eye Science ; (12): 540-544, 2021.
Article in Chinese | WPRIM | ID: wpr-873464

ABSTRACT

@#AIM: To study the characteristics and related influencing factors of subfoveal choroidal thickness(SFCT)changing with time in children with myopia. <p>METHODS: Totally 60 eyes of 60 children with myopia aged 6-15 years old who were admitted to the Eye Hospital of Nanchang University from July 2018 to September 2018 were included. IOP, axial length, equivalent spherical degree and SFCT were measured at baseline and 6mo of follow-up, respectively. Analyze the changes of the above-mentioned eye parameters and related influencing factors of SFCT. <p>RESULTS: Compared with baseline, follow-up for 6mo, myopic children's SFCT decreased by 8±14μm(<i>P</i><0.001), the axial length increased by 0.15±0.16mm(<i>P</i><0.001), and the equivalent spherical degree increased by -0.34±0.33D(<i>P</i><0.001), and there is a significant correlation between the SFCT change value and the axial length and equivalent spherical power change value(<i>r</i>s=-0.701, 0.633, both <i>P</i><0.001), indicating faster axial growth and myopia increasing is related to more thinning of the membrane thickness. Multiple linear regression analysis shows that SFCT is significantly correlated with axial length(<i>P</i>=0.001).<p>CONCLUSION: The SFCT of myopic children becomes thinner over time, and the axial length is negatively correlated with SFCT, and children with faster axial growing and greater myopia increasing are usually accompanied by more SFCT thinning.

10.
International Eye Science ; (12): 540-544, 2021.
Article in Chinese | WPRIM | ID: wpr-873463

ABSTRACT

@#AIM: To study the characteristics and related influencing factors of subfoveal choroidal thickness(SFCT)changing with time in children with myopia. <p>METHODS: Totally 60 eyes of 60 children with myopia aged 6-15 years old who were admitted to the Eye Hospital of Nanchang University from July 2018 to September 2018 were included. IOP, axial length, equivalent spherical degree and SFCT were measured at baseline and 6mo of follow-up, respectively. Analyze the changes of the above-mentioned eye parameters and related influencing factors of SFCT. <p>RESULTS: Compared with baseline, follow-up for 6mo, myopic children's SFCT decreased by 8±14μm(<i>P</i><0.001), the axial length increased by 0.15±0.16mm(<i>P</i><0.001), and the equivalent spherical degree increased by -0.34±0.33D(<i>P</i><0.001), and there is a significant correlation between the SFCT change value and the axial length and equivalent spherical power change value(<i>r</i>s=-0.701, 0.633, both <i>P</i><0.001), indicating faster axial growth and myopia increasing is related to more thinning of the membrane thickness. Multiple linear regression analysis shows that SFCT is significantly correlated with axial length(<i>P</i>=0.001).<p>CONCLUSION: The SFCT of myopic children becomes thinner over time, and the axial length is negatively correlated with SFCT, and children with faster axial growing and greater myopia increasing are usually accompanied by more SFCT thinning.

11.
International Eye Science ; (12): 549-552, 2021.
Article in Chinese | WPRIM | ID: wpr-873462

ABSTRACT

@#AIM: To investigate the vision-related quality of life after corneal refraction surgery of the new recruits in a navy unit. <p>METHODS:New recruits in a navy unit during 2014-2016 were interviewed druing recheck ophthalmic examination through a questionnaire, including basic data and the Visual Function Questionnaire-25(VFQ), and also checking the visual acuity, contrast sensitivity(CS), optometry.<p>RESULTS: Among 2 760 new recruits, 340 persons(12.32%)had undergone corneal refractive surgeries, which included corneal stroma surgery(89.71%)and corneal surface surgery(10.29%). According to the preoperative myopia spherical equivalent, the subjects were divided into three groups: mild myopia group(≤-3.00D, 134 cases), moderate myopia group(>-3.00 D to < -6.00D, 159 cases), and high myopia group(≥-6.00D, 47 cases). The postoperation spherical equivalent range ±1.0D is 128 cases(95.52%), 153 cases(96.23%), 38 cases(80.85%)in mild myopia group, moderate myopia group and high myopia group respectively. The contrast sensitivity(CS)were statistically significant among three different diopter groups under natural light and glare conditions(<i>P</i><0.05), the CS of the low myopia group and moderate myopia group were higher than in high myopia group(<i>P</i><0.05). The visual-related quality of life scores were statistically significant among three group(<i>P</i><0.05). The scores of low(6.25±8.61)points and moderate myopia groups(6.87±8.51)points were significantly better than that of high myopia group(9.84±9.76)points(<i>P</i><0.05).<p>CONCLUSION:The vision-related quality of life in new recruits after corneal refractive surgery in the unit is related with preoperative diopter of refractive error. The vision-related quality of life in recruit with lower diopter are better than in recruit with higher dioper.

12.
Article in Chinese | WPRIM | ID: wpr-906496

ABSTRACT

Objective:To explore the difference in antibacterial mechanism between <italic>Coptis chinensis</italic> and<italic> </italic>its<italic> </italic>flower stalk based on secondary metabolites and network pharmacology. Method:Based on the ultraperformance liquid chromatography mass spectrometry(UPLC-MS/MS) detection platform,the secondary metabolites database of <italic>C. chinensis</italic> and its flower stalk(MWDB) was built. The common database of metabolites information and the multivariate statistical analysis were used to study the differences of secondary metabolites between <italic>C. chinensis</italic> and its flower stalk and screen out 18 metabolites of<italic> </italic>the<italic> </italic>flower stalk and 11 metabolites of <italic>C. chinensis</italic> with a high content. BATMAN-TCM database was used to obtain the targets of component action,and their corresponding genes were inquired in the UniProt database. GeneCards was retrieved for antimicrobial genes,and the intersection genes of components and antimicrobials were obtained on Venny platform. Through DAVID gene ontology(GO) enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis,the mechanism of its action was predicted,and the results were visualized through histogram and advanced bubble diagram drawn by GraphPad Prism software and OmicShare database. The protein-protein interaction(PPI) network was constructed by STRING, database and the component-target-pathway network was constructed by Cytoscape 3.7.2 software. The antibacterial differences were compared based on the results of network pharmacology analysis. Result:Through network pharmacology,the antibacterial active components of <italic>C. chinensis</italic> were 5 fewer than that of the flower stalk,55 more antibacterial targets than that of the flower stalk; quercetin and berberine were predicted to be the common components of the antagonistic action of <italic>C. chinensis </italic>and the flower stalk. Key genes involved in antimicrobial action were p38 Mitogen-activated protein kinase 14(MAPK14),catalase(CAT); malaria and Toll-like receptor signaling pathway were different key pathways involved in antimicrobial activity. Conclusion:<italic>C. chinensis </italic>and the flower stalk mainly exert the antibacterial effect in a multi-target and multi-pathway manner,which can offer new ideas and clues for the study of antibacterial mechanism of<italic> C. chinensis</italic> and the flower stalk,and provide a new development direction for the comprehensive development and rational application of the flower stalk resources.

13.
Article in Chinese | WPRIM | ID: wpr-905838

ABSTRACT

Objective:To observe the clinical efficacy of Qingzhenfang for plasmoby (chronic urticaria), and to investigate its effect on cellular immune function. Method:One hundred and thirty-two cases patients were divided into control group and observation group evenly according to random number table. The 60 patients in control group finished the study because of 6 cases of dropout, loss of follow-up and withdrawal, and 62 patients in observation group finished the study. Patients in both groups got Yiebastine tablets, 10-20 mg/time, 1 time/day. Patients in control group additionally got Piminxiao capsule, 4 grains/time, 3 times/day, while patients in observation additionally got Qingzhenfang, 1 dose/day. The treatment continued for 8 weeks in both groups. Before the treatment, and at the second, fourth, and eighth week after treatment, scores of urticaria activity for 7 days (USA7) and total symptom score (TSS) were graded. Before and after treatment, scores of chronic urticaria quality of life scale (CU-Q2oL) and syndrome of rheumatic fever were graded. A follow-up of 3 months was conducted for the patients whose score of USA7 was less than 7 to record the recurrence. Complement 3 and 4 (C3, C4), CD4<sup>+</sup>, CD8<sup>+</sup> cells were detected, and Th17/ CD4<sup>+</sup> and Treg/ CD4<sup>+</sup>, CD4<sup>+</sup>/CD8<sup>+</sup> and Th17/Treg were calculated. Levels of peripheral blood interleukin-10 (IL-10), IL-17 and IL-23 were detected, and safety was evaluated after the treatment. Result:At the second, fourth and eighth week after the treatment, scores of USA7, TSS, CU-Q2oL and syndrome of rheumatic fever in observation group were lower than those in the control group (<italic>P</italic><0.01). Levels of C3, C4, CD4<sup>+</sup>, Treg, CD4<sup>+</sup>/CD8<sup>+</sup>and IL-35 in observation group were higher than those levels detected in control group (<italic>P</italic><0.01), while levels of CD8<sup>+</sup>, Th17, Th17/Treg, IL-10, IL-17 and IL-23 were lower than those in the control group (<italic>P</italic><0.01). Recurrence rate was 25.58% (11/43) in observation group, lower than 48.48% (16/33) in control group (<inline-formula><alternatives><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:msup><mml:mrow><mml:mi>χ</mml:mi></mml:mrow><mml:mrow><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math><graphic specific-use="big" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="alternativeImage/19F4CEA3-4719-4fe6-AFE8-81E481AA497E-M002.jpg"><?fx-imagestate width="3.30199981" height="3.64066648"?></graphic><graphic specific-use="small" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="alternativeImage/19F4CEA3-4719-4fe6-AFE8-81E481AA497E-M002c.jpg"><?fx-imagestate width="3.30199981" height="3.64066648"?></graphic></alternatives></inline-formula>=4.276, <italic>P</italic><0.05), and the clinical efficacy in observation group was superior to that in control group (<italic>Z</italic>=2.021, <italic>P</italic><0.05). Conclusion:Yaoyi Qingzhenfang can control the degree of disease and improve the quality of life for patients with chronic urticaria, with superior clinical efficacy. In addition, it can reduce recurrence rate, increase the levels of C3, C4, regulate cellular immune function, and reduce immune inflammatory response, so it is worthy of further clinical research and use.

14.
Chinese Journal of Traumatology ; (6): 214-220, 2021.
Article in English | WPRIM | ID: wpr-888411

ABSTRACT

PURPOSE@#Previous studies usually examine the associations between psychological distresses and quality of life (QOL) with a variable-centred approach, while little is known about the effect of the individual variance in time-varying changes of psychological distresses on QOL. Therefore, this study aimed to examine whether individual variance in psychological distresses during the early phases post-earthquake would develop different QOL's levels among adolescent survivors 10-year after the Wenchuan earthquake.@*METHODS@#Data were extracted from the Wenchuan Earthquake Adolescent Health Cohort Study. The current study included 744 adolescent survivors who effectively completed surveys at 6 months, 24 months, and 10 years after the earthquake. Self-report questionnaires were administered to collect information on socio-demographic characteristics, earthquake exposure, life events, anxiety symptoms, depressive symptoms, posttraumatic stress symptoms (PTSS), and QOL. Data were analysed using hierarchical multiple regression.@*RESULTS@#Trajectories of psychological distresses were classified as follow: resistance (anxiety 40.73%; depression 54.70%; PTSS 74.46%), recovery (anxiety 17.20%; depression 9.27%; PTSS 10.35%), delayed dysfunction (anxiety 10.35%; depression 18.15%; PTSS 6.18%), and chronicity (anxiety 31.72%; depression 17.88%; PTSS 9.01%). After controlling covariates, hierarchical multiple regression only revealed that the anxiety trajectory with delayed dysfunction remained significantly predictive for four domains of QOL (physical health, psychological health, social relationships, and environment).@*CONCLUSION@#The current study highlights the importance of focusing on the variations in trajectories of anxiety symptoms among disaster survivors and providing individualized mental health services to improve survivors' QOL.

15.
Journal of Clinical Hepatology ; (12): 1806-1810., 2021.
Article in Chinese | WPRIM | ID: wpr-886335

ABSTRACT

ObjectiveTo establish a droplet digital PCR (ddPCR) method for detecting hepatitis B virus (HBV) covalently closed circular DNA (cccDNA). MethodsHBV cccDNA standard substance was constructed, and HBV cccDNA primers and probes were designed based on the structural differences between HBV cccDNA and relaxed circular DNA (rcDNA). HBV plasmid was amplified to obtain HBV cccDNA standard substance, and a ddPCR detection method was established with the standard substance after gradient dilution as the template for HBV cccDNA detection; the limit of detection and repeatability of this method were analyzed. Liver tissue samples were collected from 20 patients who attended Beijing YouAn Hospital, Capital Medical University, from June 2017 to October 2020, all of whom were diagnosed with HBV infection, and DNA of the samples was extracted and digested with plasmid-safe ATP-dependent DNA enzyme to obtain HBV cccDNA template; the ddPCR detection method was evaluated in clinical samples and was compared with the quantitative real-time PCR (qPCR) detection method. The chi-square test was used for comparison of categorical data between the two groups. ResultsThe HBV cccDNA detection method based on ddPCR was established, which accurately detected HBV cccDNA in standard substance after gradient dilution, with a limit of detection of 1 copy/μl, and the coefficients of variation of 1×103, 1×102, and 1×101 copies/μl standard substances were 441%, 3.98%, and 5.09%, respectively. HBV cccDNA was detected in the samples of 20 patients with HBV infection; the ddPCR detection method detected HBV cccDNA in 17 patients, with a positive rate of 85%, while the qPCR detection method detected HBV cccDNA in 11 patients, with a positive rate of 55%, and there was a significant difference between the two methods (χ2=4.286, P=0038). ConclusionThe established ddPCR method for detecting HBV cccDNA has a low limit of detection and good repeatability, which provides an effective tool for further clinical detection.

16.
Chinese Medical Journal ; (24): 318-325, 2021.
Article in English | WPRIM | ID: wpr-878045

ABSTRACT

BACKGROUND@#Methylene blue is the most commonly used tracer for sentinel lymph node (SLN) biopsy (SLNB) in China. This study aimed to investigate the feasibility of clinical application of SLNB using methylene blue dye (MBD) for early breast cancer and the prognosis of patients with different SLN and non-SLN statuses.@*METHODS@#We retrospectively analyzed the clinicopathological data of patients with early breast cancer treated at the Peking University First Hospital between 2013 and 2018. We calculated the SLN identification rate (IR) in SLNB with MBD and the false-negative rate (FNR), and analyzed the prognosis of patients with different SLN and non-SLN statuses using Kaplan-Meier curves.@*RESULTS@#Between January 2013 and December 2018, 1603 patients with early breast cancer underwent SLNB with MBD. The SLN IR was 95.8% (1536/1603). Two SLNs (median) were detected per patient. There were significant differences in FNR between patients with SLN micrometastasis and macrometastasis (19.0% vs. 4.5%, χ2 = 12.771, P < 0.001). Chi-square test showed that there were significant differences in SLN successful detection rates among patients with different vascular tumor embolism status (96.3% vs. 90.8%, χ2 = 9.013, P = 0.003) and tumor (T) stages (96.6% vs. 94.1%, χ2 = 5.189, P = 0.023). Multivariate analysis showed that vascular tumor embolism was the only independent factor for SLN successful detection (odds ratio: 0.440, 95% confidence interval: 0.224-0.862, P = 0.017). Survival analysis showed a significant difference in disease-free survival (DFS) between patients with non-SLN metastasis and patients without non-SLN metastasis (P = 0.006).@*CONCLUSION@#Our single-center data show that, as a commonly used tracer in SLNB in China, MBD has an acceptable SLN IR and a low FNR in frozen sections. This finding is consistent with reports of dual tracer-guided SLNB. Positive SLNs with non-SLN metastasis are associated with DFS.


Subject(s)
Breast Neoplasms/surgery , China , Humans , Lymph Nodes , Methylene Blue , Retrospective Studies , Sentinel Lymph Node Biopsy
17.
Journal of Experimental Hematology ; (6): 1595-1600, 2021.
Article in Chinese | WPRIM | ID: wpr-922301

ABSTRACT

OBJECTIVE@#To investigate the procedure of pre-transfusion testing and transfusion strategy of patients with multiple myeloma (MM) treated by daratumumab (DarA).@*METHODS@#The blood samples of MM patients before and after DarA treatment from the Fifth Affiliated Hospital of Sun Yat-sen University were collected, and the ABO/Rh blood group antigen identification and DAT test results were compared. The results of antibody screening and cross matching of the patients before and after inactivation of red blood cells with 0.2 mol/L dithiothreitol (DTT) were compared and analyzed.@*RESULTS@#ABO/Rh blood group antigen typing showed no affecting in patients after treated by DarA; the result of DAT test showed negative. Irregular antibody screening showed that all the three cells(Ⅰ, Ⅱ and Ⅲ) were positive(1+~2+) and the self-control was negative. By microcolumn agglutination method, the main side of the multi-bag of blood showed no matched, while the secondary side showed all identical. After treated by DTT solution, the cross matching results in reagent red blood cells and the red blood cells of blood donors were both consistent, and the irregular antibody screening was negative. The K(+)O type erythrocytes used in parallel control were transformed into K(-)O type erythrocytes after DTT treatment. However, there was no significant changes in E(+) O type erythrocytes before and after DTT treatment. There was no condensation on the primary and secondary side of the condensed amine method. The primary and secondary sides of blood matching by saline method showed negative.@*CONCLUSION@#After treated by DarA, cross matching results from microcolumn agglutination method can be interfered by the residual drug antibody in MM patients, while the interference was eliminated in the presence of 0.2 mol/L DTT solution. However, no disturbance was observed when using condensed amine method or saline method. Therefore, corresponding transfusion procedures should be selected according to the emergency degree of blood transfusion to ensure the safety and timeliness of blood transfusion.


Subject(s)
Antibodies, Monoclonal , Blood Transfusion , Dithiothreitol , Humans , Multiple Myeloma/therapy
18.
International Eye Science ; (12): 540-544, 2021.
Article in Chinese | WPRIM | ID: wpr-923264

ABSTRACT

@#AIM: To study the characteristics and related influencing factors of subfoveal choroidal thickness(SFCT)changing with time in children with myopia. <p>METHODS: Totally 60 eyes of 60 children with myopia aged 6-15 years old who were admitted to the Eye Hospital of Nanchang University from July 2018 to September 2018 were included. IOP, axial length, equivalent spherical degree and SFCT were measured at baseline and 6mo of follow-up, respectively. Analyze the changes of the above-mentioned eye parameters and related influencing factors of SFCT. <p>RESULTS: Compared with baseline, follow-up for 6mo, myopic children's SFCT decreased by 8±14μm(<i>P</i><0.001), the axial length increased by 0.15±0.16mm(<i>P</i><0.001), and the equivalent spherical degree increased by -0.34±0.33D(<i>P</i><0.001), and there is a significant correlation between the SFCT change value and the axial length and equivalent spherical power change value(<i>r</i>s=-0.701, 0.633, both <i>P</i><0.001), indicating faster axial growth and myopia increasing is related to more thinning of the membrane thickness. Multiple linear regression analysis shows that SFCT is significantly correlated with axial length(<i>P</i>=0.001).<p>CONCLUSION: The SFCT of myopic children becomes thinner over time, and the axial length is negatively correlated with SFCT, and children with faster axial growing and greater myopia increasing are usually accompanied by more SFCT thinning.

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Article in Chinese | WPRIM | ID: wpr-873188

ABSTRACT

Objective:To investigate the effect of different dose of Realgar compatible with Indigo Naturalis on the transitional constituents of Indigo Naturalis in rat serum based on the compatibility of Qinghuangsan.Method:Indigo Naturalis test solution, the drug-containing serum of three different proportions of Qinghuangsan (10 g of Indigo Naturalis compatible with 52.5, 105, 210 mg of Realgar for group A, B and C, respectively) and blank serum were detected by UPLC-Q-TOF-MS/MS, in combination with the chemical components identified in Indigo Naturalis test solution, the differences of transitional constituents of Indigo Naturalis in rat serum from the group A, B and C were analyzed. HL-60 cells (human leukemia cells) were treated with the three groups of Qinghuangsan drug-containing serum and the effect of drug-containing serum on the activity of HL-60 cells was detected by cell counting kit-8 (CCK-8) assay.Result:A total of 19, 22, 25 of transitional constituents were detected in Qinghuangsan drug-containing serum from group A, B and C, respectively. The three groups of drug-containing serum all contained 5 prototype components from Indigo Naturalis test solution, including tryptanthrin, indigo, indirubin, 2-aminobenzoic acid and N-phenyl-2-naphthylamine, respectively. The results of CCK-8 assay showed that Qinghuangsan drug-containing serum of group C had the strongest inhibitory effect on HL-60 cells.Conclusion:After fixed Indigo Naturalis dose, with the increase of Realgar dose, the transitional constituents in rat serum increase and the inhibitory effect on HL-60 cells also gradually enhances, which indicates that Realgar may promote the absorption of active components in Indigo Naturali in vivo, thus enhance the efficacy, further explains the compatibility law and pharmacodynamic material basis of different proportions of Realgar and Indigo Naturalis.

20.
Article in Chinese | WPRIM | ID: wpr-846194

ABSTRACT

Objective: To study the chemical constituents from Sinopodophyllum hexandrum and their antitumor activities. Methods: The constituents were separated by chromatography of silica gel, ODS, Sephadex LH20 and pre-TLC. Their structures were elucidated by spectroscopic means. The in vitro cytotoxic activities of the isolated compounds were studied by MTT method. Results: Nine compounds were isolated and identified as 8,2'-diprenylquercetin 3-methyl ether-4'-O-β-D-glucoside (1), 8,2'-diprenyl quercetin-3- methylether (2), 5,7,4'-trihydroxy-3'-(3-methylbut-2-enyl)-3-methoxy flavone (3), 8-prenylkaempferol (4), sophoflavescenol (5), podoverine A (6), sinoflavonoid K (7), diosmetin (8) and acacetin (9). Conclusion: Compound 1 is a new compound named sinoflavonoid glycosides A, and compounds 5-9 are isolated from S. hexandrum for the first time. Compounds 1-5 show cytotoxicities against HeLa cells with IC50 of 42.6, 46.9, 26.9, 16.1 and 31.2 μmol/L, respectively.

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