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1.
Chinese Journal of Endemiology ; (12): 273-277, 2020.
Article in Chinese | WPRIM | ID: wpr-866103

ABSTRACT

Objective:To understand the temporal and spatial characteristics of fluoride in rural drinking water in Jinan City.Methods:From 2015 to 2018, the factory water and terminal water samples of centralized rural water supply system were collected from all townships (streets) in 7 agriculture-related districts (counties) in Jinan City during dry season and high water season. Water samples were collected, stored, transported and tested in accordance with the "Standard Examination Methods for Drinking Water" (GB/T 5750-2006). The time, region, endemic areas (Zhangqiu, Jiyang, Shanghe, Licheng, Changqing) and non-endemic areas (Pingyin, Tianqiao) of drinking water type fluorosis, water periods (dry season and high water season), water samples (factory water and terminal water), water sources (shallow well water, deep well water, river water and reservoir water) of water fluorine were analyzed descriptively.Results:A total of 1 638 water samples were collected from 2015 to 2018, the median of water fluorine was 0.400 mg/L, ranging from 0.002 to 2.700 mg/L. The differences in water fluorine of different year (0.400, 0.430, 0.490, 0.360 mg/L) were statistically significant ( H = 33.907, P < 0.01). Total water fluorine excess rate was 5.49% (90/1 638), there were 3 districts with water fluorine excess, namely Jiyang [26.11% (59/226)], Zhangqiu [6.12% (30/490)] and Licheng [0.53% (1/188)]. The median of water fluorine in endemic areas (1 398 samples) was 0.420 mg/L, the non-endemic areas (240 samples) was 0.320 mg/L, and the water fluorine in endemic areas was significantly higher than that in non-endemic areas ( Z =-6.490, P < 0.01). The water fluorine excess rate in endemic areas was 6.44% (90/1 398), and there was no water fluorine excess in non-endemic areas. The median of water fluorine in both dry season (819 samples) and high water season (819 samples) was 0.400 mg/L. The median of water fluorine of factory water (392 samples) was 0.320 mg/L, the terminal water (1 246 samples) was 0.450 mg/L, and the water fluorine of terminal water was significantly higher than that of factory water ( Z =-6.881, P < 0.01). The water fluorine excess rates of shallow well water and deep well water were 31.76% (47/148) and 3.82% (43/1 126), respectively, and there were no water fluorine excess in river water and reservoir water. Conclusions:Fluoride of drinking water in rural areas of Jinan City exceeds the standard, mainly concentrated in Jiyang District. The water fluorine in endemic areas is higher than that in non-endemic areas, water fluorine of terminal water is higher than that of factory water, shallow well water and deep well water as the sources of water has excessive water fluoride, and the general survey and detection of fluoride content in residents' drinking water should be continuously carried out.

2.
Acta Pharmaceutica Sinica ; (12): 1837-1844, 2019.
Article in Chinese | WPRIM | ID: wpr-780314

ABSTRACT

Calcium-binding protein S100A9 is closely related to inflammation and tumor invasion, and is one of the specific markers of myeloid-derived suppressor cells (MDSC). In this study, a recombinant polypeptide vaccine CTB-S100A9 targeting mouse calcium-binding protein S100A9 was constructed by fusion cholera toxin B subunit (CTB) with S100A9 gene. The CTB-S100A9 fusion protein was expressed in E coli. and purified by Ni+ affinity chromatography. Vaccinate the purified recombinant CTB-S100A9 protein supplemented with aluminum hydroxide adjuvant can break the autoimmune tolerance and produce high titer of S100A9 antibody in mice. Moreover, the S100A9 antibody produced by CTB-S100A9 vaccination is more specific and does not cross-react with S100A8. In the mouse 4T1 breast cancer model, CTB-S100A9 vaccination not only has significant tumor prevention effects, but also has significant tumor therapeutic effects. In addition, CTB-S100A9 significantly inhibited lung metastasis in 4T1 mice breast cancer model. Further analysis by flow cytometry showed that CTB-S100A9 vaccination can significantly reduce the tumor induced Treg cells and granulocyte-derived MDSC in 4T1 mice model, and reverse the tumor immunosuppressive environment, thereby promote the anti-tumor efficacy. The animal experiments in this study were carried out under the animal care guidelines approved by the Animal Ethics Committee of the Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine. This study shows that CTB-S100A9 is a good recombinant vaccine that targets the tumor immune-suppression environment and has great potential for the future clinical application.

3.
Article in Chinese | WPRIM | ID: wpr-743696

ABSTRACT

Objective: To construct a novel tissue engineering complex, BMSCs sheet-RADA16 scaffold, by combining cell sheet and self-assembled peptides.. Methods: The self-assembled peptide RADA16 scaffold was wrapped with the BMSCs cell sheet. The morphology of the cells and the complex were observed by SEM and confocal laser microscopy, and the proliferation of cells was assessed by CCK-8. The osteogenic differentiation of BMSCs was examined by detection of related gene expression with RT-PCR. Results: Compared with the BMSCs cell sheet, the numbers of cells on RADA16 scaffold growth rapidly at 3 rd-8 th day, and BMSCs were more on the scaffold than those on the cell sheet (P<0. 05) . RT-PCR results showed that the expression level of osteogenesis-related genes was higher in the complex (P<0. 05) . Conclusion: BMSCs Sheet-RADA16 Scaffold may promote proliferation and osteogenic differentiation of BMSCs.

4.
Article in Chinese | WPRIM | ID: wpr-697489

ABSTRACT

Objective: To evaluate the effect of minimally invasive surgery on acceleration orthodontic tooth movement by meta-analysis. Methods: All literatures about minimally invasive surgery accelerating tooth moment were searched from the database in general. The literatures were screened according to the correlation and the inclusion criteria, included literatures were analyzed by RevMan 5. 3. Results: 5 articles including 85 patients were included in this review, 4 of which reported that minimally invasive surgery can accelerate orthodontic tooth movement, but 1 reported no statistical difference in alleviating mandibular anterior crowding between minimally invasive surgery acceleration orthodontic group and conventional orthodontic group. Higher tooth movement rate was found with the minimally invasive surgical procedures by a weighted mean difference of 0. 70 mm in 1 month of canine retraction (WMD = 0. 7: 95% CI(0. 57, 0. 82); P< 0. 001) and by a weighted mean difference 1. 31 mm in 2 months (WMD = 1. 31: 95% CI (0. 69, 1. 92), P< 0. 001). No obvious adverse effects were observed in periodontal condition, pain, satisfaction, root resorption and anchorage control. Conclusion: According to current studies, minimally invasive surgery can accelerate single tooth movement, but the evidence is insufficient to prove that the entire orthodontic treatment time can be shortened.

5.
Article in Chinese | WPRIM | ID: wpr-703144

ABSTRACT

Objective To investigate the association between the level of polymorphism of APOE gene and cognitive impairment in patients with CNS demyelinating diseases. Methods 56 patients with central nervous system demyelinating disease were applied APOE genotyping,MoCA and expanded disability status (EDSS) scale score. Patients with MOCA scores <26 were divided into cognitive impairment group, and those with MOCA scores ≥26 were divided into normal cognitive preserved group. Results The probability of cognitive dysfunction in patients with central nervous system demyelinating diseases was 53.57%. There was no significant difference in age, gender, and disease duration between the CI group and the CP group(P>0.05), the difference in age and education among groups is statistically significant (P<0.05). There was no statistical significance in the difference in age, sex, education years and EDSS score between APOEε4 gene positive group and APOEε4 gene negative group (P<0.05). The difference of visual space and attention between different cognitive domains is statistically significant(P<0.05). Years of schooling is a risk factor for cognitive dysfunction in patients with central nervous system demyelinating disease(P<0.01). Conclusion The central nervous system demyelinating disease is impaired cognitive function. Patients with APOEε4 gene positive are more severely impaired in visual space and attention than patients with negative APOEε4 gene.Years of education are the risk factors of cognitive dysfunction in patients with central nervous system demyelinating disease. The course of disease and disabled function may not be significant related to cognitive impairment.

6.
Chinese Journal of Immunology ; (12): 866-871, 2018.
Article in Chinese | WPRIM | ID: wpr-702833

ABSTRACT

Objective:To explore effects and mechanism of resveratrol on apoptosis of vascular smooth muscle cell in diabetic rats. Methods: The mRNA level of monocyte chemoattractant protein 1 (MCP1),macrophage migration inhibitory factor (MIF) and in-terleukin 18 (IL-18) was detected by quantitative real-time reverse transcription PCR (qRT-PCR). Myocardial fibrosis was analyzed by hematoxylin-eosin (HE) staining and Masson staining. Apoptosis was tested by flow cytometry. The expression of Cleaved caspase-3, Cleaved caspase-9,B cell lymphoma 2 (Bcl-2),Bcl-2-associated X protein (Bax),PI3K,p-PI3K,AKT and p-AKT was measured by Western blot. Results: The mRNA levels of MCP1,MIF and IL-18 in STZ-induced diabetic were reduced by resveratrol (P<0. 05). The aggravation of myocardial fibrosis in STZ-induced diabetic rats was ameliorated by resveratrol. Apoptosis of vascular smooth muscle cell in STZ-induced diabetic rat group was higher than control group (P<0. 05 ) . Compared with STZ-induced diabetic rat group, apoptosis of vascular smooth muscle cell in STZ+ resveratrol group was decreased (P<0. 05). What′s more,the expression of Cleaved caspase-3,Cleaved caspase-9 and Bax in STZ-induced diabetic rats were inhibited by resveratrol(P<0. 05). And the expression of Bcl-2 in STZ-induced diabetic rats was elevated by resveratrol(P<0. 05). Compared with control group,the rate of p-PI3K/PI3K and p-AKT/AKT in STZ-induced diabetic rat group was decreased (P<0. 05). The rate of p-PI3K/PI3K and p-AKT/AKT in STZ+ resveratrol group was higher than STZ-induced diabetic rat group ( P<0. 05). Conclusion: Resveratrol represses apoptosis of vascular smooth muscle cell in STZ-induced diabetic rats through activating of PI3K/AKT signal pathway.

7.
Chinese Journal of Immunology ; (12): 531-536,548, 2018.
Article in Chinese | WPRIM | ID: wpr-702769

ABSTRACT

Objective:To explore effects and mechanism on glucolipid metabolism of liver of ginsenoside Rb1 in diabetic rats.Methods:Diabetic rat model was induced by streptozotocin (STZ).Hepatic pathological changes were observed by hematoxylin-eosin (HE) staining.Apoptosis was analyzed through Terminal deoxynucleotidyl transferase-mediated dUTP nick labeling (TUNEL) staining.Blood glucose was determined with glucometer.The level of insulin and C peptide was tested by radioimmunoassay.Total cholesterol,low density lipoprotein cholesterol,high-density lipoprotein cholesterol,triglyceride was detected by full automatic biochemical analyzer.The expression of JNK signal pathway related proteins JNK1 and c-Jun was measured by Western blot.The expression of inflammatory factor IL-6,IL-1β and TNF-α was detected by immunohistochemistry.Results:Liver injury was ameliorated by ginsenoside Rb1 in diabetic rats.Compared with control group,the level of blood glucose in STZ-induced diabetic rat group was increased with attenuated level of insulin and C peptide (P<0.05).Compared with STZ-induced diabetic rat group,the level of blood glucose in STZ+ginsenoside Rb1 group was decreased with enhancive level of insulin and C peptide(P<0.05).Compared with control group,the level of total cholesterol,triglyceride and low density lipoprotein cholesterol in STZ-induced diabetic rat group was elevated with attenuated level of high-density lipoprotein cholesterol(P<0.05).Compared with STZ-induced diabetic rat group,the level of total cholesterol,triglyceride and low density lipoprotein cholesterol in STZ+ginsenoside Rb1 group declined with elevated level of high-density lipoprotein cholesterol(P<0.05).The relative expression of JNK1 and c-Jun in STZ-induced diabetic rat group was higher than control group (P<0.05).The relative expression of JNK1 and c-Jun in STZ+ginsenoside Rb1 group was lower than STZ-induced diabetic rat group(P<0.05).Compared with control group,the expression of IL-6,IL-1β and TNF-α in STZ-induced diabetic rat group was enhanced (P<0.05).The expression of IL-6,IL-1β and TNF-α in STZ+ginsenoside Rb1 group was lower than STZ-induced diabetic rat group(P<0.05).Conclusion:Ginsenoside Rb1 ameliorates liver injury,abnormal glucolipid metabolism and inflammatory response of liver through inhibition of JNK signal pathway in diabetic rats.

8.
Chinese Journal of Geriatrics ; (12): 854-857, 2017.
Article in Chinese | WPRIM | ID: wpr-611147

ABSTRACT

Objective To investigate the incidence and outcome of hyponatremia in patients with severe dementia.Methods Dementia patients were retrospectively analyzed in 80 cases,including 45 patients with severe dementia complicating 30 cases of hyponatremia,and 35 patients with mild and moderate dementia complicating 5 cases of hyponatremia.The incidence rate of dementia patients complicating hyponatremia and its fatality rate were analyzed by x2 tests.The effect of dementia severity on hyponatremia and fatality rate was studied.Results The incidence rate of hyponatremia was higher in severe dementia patients (30 cases,66.7 %) than in mild-moderate dementia patients (5 cases,14.3 %) (x2 =21.95,P < 0.01).The fatality rate of hyponatremia in severe dementia patients was higher(43.3%)than in patients with normal serum sodium(33.3 %)(x2=0.25,P>0.05).Conclusions Hyponatremia is more likely to occur in patients with severe dementia.However,hyponatremia does not affect survival time in the severe dementia patients.

9.
Article in Chinese | WPRIM | ID: wpr-508104

ABSTRACT

Objective Inflammation is a defensive reaction of body , but excessive inflammatory response can lead to physi-cal injury.The aim of this study was to explore the effects of luteolin on the secretion of inflammatory cytokines from lipopolysaccharide (LPS) and interferon-g(IFN-γ) activated RAW264.7 cells. Methods RAW264.7 cells were divided into 5 groups: control group (without any medicine), M1 group (polarized M1 cells activated by final concentration of 10 ng/mL LPS+20 ng/mL IFN-γ), M1+5L group (simultaneous activation of LPS and IFN-γplus final concentration of 5μmol/L luteolin), M1+10L group(simultaneous activa-tion of LPS and IFN-γplus 10μmol/L luteolin), M1+20L group(simultaneous activation of LPS and IFN-γplus 20μmol/L luteolin). The cell morphological transformation was observed by laser confocal microscope ;the mRNA levels of iNOS , IL-1βand IL-6 were test-ed by real-time quantitative PCR respectively;the secretion levels of TNF-αand IL-6 in culture supernatant were detected by ELISA;the changes of p-STAT3 (ser727) protein pathways were examined by western blot. Results Cellular morphology of activated RAW 264.7 cells changed obviously .Compared with the control group , the mRNA levels of iNOS, IL-1βand IL-6 decreased significantly in the other 4 groups(P<0.05).The iNOS level in M1+20L group significantly de-creased compared with M1 group[(29.52±3.07) vs (98.91±10.65), P<0.01].As to IL-1βlevel, it decreased significantly in M1+10L group(78.38±8.65) and M1+20L group(41.59±6.80) compared with M1 group(110.69±4.12)(P<0.05).While the IL-6 levels decreased significantly in M1+5L group(177.51±19.28), M1+10L group (106.14±5.63), M1+20L group(27.15±1.26), compared with M1 group(394.10±33.47)(P<0.05).LPS+IFN-γcould induce in-creased p-STAT3 (ser727) expression in M1 phenotype of RAW264.7 cells which was proved by its significant increase in M 1 group, M1+5L group and M1+10L group compared with control group (P<0.05).In comparison to M1 group, p-STAT3-ser expression in M1 phenotype downregulated in M1+5L group, M1+10L group, M1+20L group(P<0.05), along with dose-dependent characteristic.Com-pared with control group, the levels of IL-6 and TNF-αincreased significantly in M1 group, M1+5L group and M1+10L group.Com-pared with M1 group, the levels of IL-6 and TNF-αdecreased significantly in M1+5L group, M1+10L group and M1+20L group(P<0.05) , in which IL-6 showed concentration independence and TNF-αshowed no concentration independence . Conclusion Luteolin inhibits the secretion of pro-inflammatory cytokines through the down-regulation of p-STAT3 so as to exert anti-inflammatory effects .

10.
Article in Chinese | WPRIM | ID: wpr-491793

ABSTRACT

Objective To real?timely monitor the environment in the forestry schistosomiasis control project(CFSCP)area and to early warn the status of Oncomelania hupensis snails in the schistosomiasis endemic area. Methods Based on ArcGIS Engine 10.1 software,the Geographic Information System(GIS)platform of the forestry schistosomiasis project of real?time mon?itoring,early warning and emergency management in Renshou County,Sichuan Province,was designed and established. Re?sults The functions of the platform mainly included real?time monitoring of the environment in CFSCP area,and early warning of the crisis status of O. hupensis snails,as well as editing the map of snail distribution,query,spatial analysis and other GIS functions. Conclusion This platform could provide the scientific support to the forestry administrative department of the CF?SCP area.

11.
Chinese Journal of Immunology ; (12): 354-357, 2015.
Article in Chinese | WPRIM | ID: wpr-460406

ABSTRACT

Objective:To study B cells proliferation and activation induced by tumor derived-autophagosomes ( TDA). Methods:Splenocytes of mice were incubated with 10μg/ml TDAs in vitro ( with the 10μg/ml whole cell lysate as the control).At the 3rd day,the expression of MHCⅠ,Ⅱand co-stimulate molecules including CD86,CD40 were detected by flow cytometry,and B cells proliferation was detected at the 5th day.B cells were selected from spleen of mice using anti-CD43 dynabeads,and incubated with 10μg/ml TDA in vitro ( with the same concentrated whole tumor cell lysate as the control).At the 7th day,IgM in the supernatant were tested by ELISA.Results:When compared with whole cell lysate stimulated B cells,TDA efficently stimulated B cells division in vitro ( TDA group:28.6%, Whole cell lysate group:4.4%) , and significantly up-regulated the expression of MHC class Ⅰ,Ⅱ and co-stimulatory molecules (CD86 and CD40) on B cells,and enhanced the levels of total IgM secretion in vitro.Conclusion:Tumor-derived autophagosomes ( TDA) ,as a vacuole antigen vector, could stimulate B cells proliferation, activation, and increased IgM secretion in vitro.

12.
Journal of Chinese Physician ; (12): 1328-1332, 2014.
Article in Chinese | WPRIM | ID: wpr-465977

ABSTRACT

Objective To investigate the effects of alpha lipoic acid on patients with diabetic retinopathy (DR),explore the possible mechanisms,and provide experimental evidence for the treatment of DR with lipoic acid.Methods Totally 75 DR patients were randomly divided into control group and treatment group.Patients in control group received the standard hypoglycemic treatment,antihypertensive therapy,and other comprehensive treatment.Patients from the treatment group were treated with alpha lipoic acid 600 mg + 250 ml 0.9% sodium chloride injection additionally,avoiding light intravenous therapy,for a total of 4 weeks.Before and after treatment,fundus fluorescein angiography and visual inspection were detected in two groups.In addition,serum level of asymmetric dimethylarginine (ADMA) were detected with high performance liquid chromatography,serum lipid peroxidation product malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were detected to reflect the in vivo oxidative stress before and after treatment in two groups.Results Compared to the control group,visual acuity was improved significantly (P < 0.05),and total efficiency of ocular lesions-improved was increased significantly (84.21% vs 40.54%,x2 =15.28,P < 0.01).Serum levels of endogenous inhibitor of nitric oxide synthase ADMA [(0.32 ±0.13) μmol/L vs (0.51 ± 0.26) μmol/L,P < 0.05] and MDA [(3.22 ± 0.90) nmol/L vs (5.13 ± 0.42) nmol/ml,P < 0.05] were significantly decreased,while SOD levels [(72.68 ± 1.31) U/ml vs (53.16 ± 1.54) U/ml,P <0.05] were significantly increased in DR patients after alpha lipoic acid treatment.Conclusions Alpha lipoic acid had some therapeutic effects for DR patients,which mainly lied in the improvement of visual acuity and eye disease,might be related to the decrease of oxidative stress and serum level of endogenous nitric oxide synthase (NOS) inhibitor ADMA.

13.
Article in Chinese | WPRIM | ID: wpr-473998

ABSTRACT

Shanghai began to strengthen the community health service system in 1997 , and had officially en-tered the period of “connotation construction” with the core policy of family doctor system in 2011 after the period of“service framework and network establishment” and“operational mechanism reform”. Through summarizing the poli-cy files related the family doctor system and based on 2013 report on monitoring and evaluation of family doctor system in Shanghai, the paper presented the progress of the system from aspects of system coverage, signature relationship, service mode and operational mechanism, and analyzed the development bottlenecks of the system from aspects of the policy itself, service principal, service supervision and service objects. Finally, the paper proposed some suggestions in order to give some references for further development of national general practitioner system.

14.
Acta Pharmaceutica Sinica ; (12): 1336-1340, 2012.
Article in Chinese | WPRIM | ID: wpr-274657

ABSTRACT

To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups: control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.


Subject(s)
Animals , B-Cell Activating Factor , Allergy and Immunology , Metabolism , B-Lymphocytes , Cell Biology , Body Weight , Cell Differentiation , Cell Proliferation , Cells, Cultured , Female , Humans , Immunoglobulin Fc Fragments , Allergy and Immunology , Metabolism , Immunoglobulin G , Blood , Allergy and Immunology , Immunoglobulin M , Blood , Mice , Mice, Inbred ICR , Random Allocation , Recombinant Fusion Proteins , Allergy and Immunology , Metabolism , Single-Chain Antibodies , Allergy and Immunology , Metabolism , Spleen , Cell Biology
15.
Chinese Journal of Biotechnology ; (12): 901-911, 2012.
Article in Chinese | WPRIM | ID: wpr-342431

ABSTRACT

Saccharomyces cerevisiae is useful as a host for genetic engineering, since it allows the folding and glycosylation of expressed heterologous eukaryotic proteins and can be subjected to many genetic manipulations. Recent advancements in the yeast cell surface engineering developed strategies to genetically immobilize amylolytic, cellulolytic and xylanolytic enzymes on yeast cell surface for the production of fuel ethanol from biomass. We reviewed the basic principle and progress of S. cerevisiae cell-surface engineering and gave an insight into the recent technological developments in the production of bioethanol using surface engineered yeast.


Subject(s)
Biofuels , Microbiology , Ethanol , Metabolism , Fermentation , Metabolic Engineering , Methods , Recombinant Proteins , Genetics , Saccharomyces cerevisiae , Genetics , Metabolism
16.
Acta Pharmaceutica Sinica ; (12): 1336-40, 2012.
Article in Chinese | WPRIM | ID: wpr-431043

ABSTRACT

To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups: control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.

17.
Article in Chinese | WPRIM | ID: wpr-642340

ABSTRACT

Objective:To methodologically establish the lentivirus granule packaging, concentration and infection against CD34~+ cells from umbilical blood. Methods:The lentivirus system of the 3~(rd) generation was used to produce the virus. Ultrafiltration and ultracentrifugation were employed to concentrate virus. Several treatments were used to improve virus infection including in vitro amplification culture, facilitation of rest cells into cell cycle, promotion of cell adhesion and immobilization during infection, and repeat infection methods. Results:CD34~+ cells were not obviously changed by checking the expression level of CD34 marker on the cell surface after 48 h culture. After two-step concentration, virus titer was increased up to 5.06×10~7/ml, and the infection rate against CD34~+ cells from umbilical blood was increased up to 37.7%.Conclusion:Lentivirus supernatant with over 10~7/ml titer can be obtained using the above methods. Efficient infection against CD34~+ cells from umbilical blood can be achieved.

18.
Chinese Journal of Stomatology ; (12): 533-536, 2007.
Article in Chinese | WPRIM | ID: wpr-359703

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro.</p><p><b>METHODS</b>Human periodontal ligament fibroblasts were cultured in vitro. The estrogen receptors (ER)-alpha, ER-beta were detected with immunocytochemistry staining; The mRNA of ER-alpha, ER-beta were measured by reverse transcription polymerase chain reaction; The expression of ER-alpha, ER-beta protein were detected by Western blot.</p><p><b>RESULTS</b>The mRNA and protein expression of two subtype of ER were observed on human periodontal ligament fibroblasts (HPLF). The intensity of the ER-beta bands were stronger than those of ER-alpha, indicating that ER-beta expression in HPLF was higher.</p><p><b>CONCLUSIONS</b>ER may play an important role in the function of human periodontal ligament fibroblasts.</p>


Subject(s)
Adolescent , Blotting, Western , Cells, Cultured , Estrogen Receptor alpha , Genetics , Metabolism , Estrogen Receptor beta , Genetics , Metabolism , Female , Fibroblasts , Metabolism , Humans , Immunohistochemistry , Periodontal Ligament , Cell Biology , RNA, Messenger , Genetics , Reverse Transcriptase Polymerase Chain Reaction
19.
Article in Chinese | WPRIM | ID: wpr-541291

ABSTRACT

Objective: To study stress distribution and displacement by different distract directioos on mandibular corpus with finite element method. Methods:A three-dimensional finite element model of mandibular distraction osteogenesis was established.Von Mises stress as well as the displac ement of gnathion and gonion under different loads were measured. Result s:The stress and displacement were positively related with distract forc e. Von Mises stress primarily accumulated in distracted areas and were higher un der bilateral distraction parallel to the occlusial plane. Gnathion and gonion w ere positively displaced on X and Z axes and negatively displaced on Y axis. Distraction parallel to the mandibular body induced positive displaceme nt of mandible on X and Y axes and negative on Z axis. Concl usion:Transversal displacement to the opposite side is greater during un ilateral distraction, while displacement on the saggittal plane is greater durin g bilateral distraction.

20.
Article in Chinese | WPRIM | ID: wpr-556383

ABSTRACT

Objective: To study the protective effect in CCl4-induced liver injury by organoselenium from Se-enriched lactobacillus. Methods: (1) In the first series, forty-five animals were randomly divided into control (C) group, CCl4 group, CCl4 plus organoselenium group (CCl4-Se group). The liver injury was induced by abdominal injection of CCl4 every other day for 4 w. Changes of GSH-Px, CAT and SOD activities as well as MDA content in liver were estimated in the 2nd and 4th week after CCl4 injection respectively. (2) In the second series, forty-eight mice were randomly divided into C group, CCl4 group, CCl4 plus low dose organoselenium group (CCl4-LSe group) and CCl4 plus high dose organoselenium group (CCl4-HSe group). Changes of hepatocyte [Ca2+]i in animals in every group were investigated by means of confocal laser microscope on the 4th and 8th day after CCl4 injection respectively. Results: During the entire experimental period, liver MDA of CCl4 group was markedly superior to that of C and CCl4-Se groups, and the level of latter two groups was very close. The GSH-Px and CAT activities were higher in CCl4-Se group than in CCl4 group,but lower than that of C group. There were higher SOD activities in C and CCl4-Se groups compared to that in CCl4 group though without obvious difference. Average fluorescence pixels of hepatocyte [Ca2+]i in CCl4 group was 2.8 and 5.5 times higher than that of group C in the 4th and 8th day respectively,while those in CCl4-Se groups were significantly lower than those of CCl4 group, and close to C group. Conclusions: Organoselenium from Se-enriched lactobacillus, can protect hepatocyte [Ca2+]i homeostasis by reducing lipid peroxidation after CCl4 exposure.

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