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1.
Article in English | WPRIM | ID: wpr-928244

ABSTRACT

Background Ultrasound-guided continuous thoracic paravertebral block can provide pain-relieving and opioid-sparing effects in patients receiving open hepatectomy. We hypothesize that these effects may improve the quality of recovery (QoR) after open hepatectomy. Methods Seventy-six patients undergoing open hepatectomy were randomized to receive a continuous thoracic paravertebral block with ropivacaine (CTPVB group) or normal saline (control group). All patients received patient-controlled intravenous analgesia with morphine postoperatively for 48 hours. The primary outcome was the global Chinese 15-item Quality of Recovery score on postoperative day 7, which was statistically analyzed using Student's t-test. Results Thirty-six patients in the CTPVB group and 37 in the control group completed the study. Compared to the control group, the CTPVB group had significantly increased global Chinese 15-item Quality of Recovery scores (133.14 ± 12.97 vs. 122.62 ± 14.89, P = 0.002) on postoperative day 7. Postoperative pain scores and cumulative morphine consumption were significantly lower for up to 8 and 48 hours (P < 0.05; P = 0.002), respectively, in the CTPVB group. Conclusion Perioperative CTPVB markably promotes patient's QoR after open hepatectomy with a profound analgesic effect in the early postoperative period.


Subject(s)
Anesthetics, Local/therapeutic use , Double-Blind Method , Hepatectomy/adverse effects , Humans , Morphine/therapeutic use , Pain Measurement , Pain, Postoperative/etiology , Ultrasonography, Interventional
2.
Acta Physiologica Sinica ; (6): 125-133, 2022.
Article in English | WPRIM | ID: wpr-927588

ABSTRACT

Captopril can have nephrotoxic effects, which are largely attributed to accumulated renin and "escaped" angiotensin II (Ang II). Here we test whether angiotensin converting enzyme-1 (ACE1) inhibition damages kidneys via alteration of renal afferent arteriolar responses to Ang II and inflammatory signaling. C57Bl/6 mice were given vehicle or captopril (60 mg/kg per day) for four weeks. Hypertension was obtained by minipump supplying Ang II (400 ng/kg per min) during the second 2 weeks. We assessed kidney histology by periodic acid-Schiff (PAS) and Masson staining, glomerular filtration rate (GFR) by FITC-labeled inulin clearance, and responses to Ang II assessed in afferent arterioles in vitro. Moreover, arteriolar H2O2 and catalase, plasma renin were assayed by commercial kits, and mRNAs of renin receptor, transforming growth factor-β (TGF-β) and cyclooxygenase-2 (COX-2) in the renal cortex, mRNAs of angiotensin receptor-1 (AT1R) and AT2R in the preglomerular arterioles were detected by RT-qPCR. The results showed that, compared to vehicle, mice given captopril showed lowered blood pressure, reduced GFR, increased plasma renin, renal interstitial fibrosis and tubular epithelial vacuolar degeneration, increased expression of mRNAs of renal TGF-β and COX-2, decreased production of H2O2 and increased catalase activity in preglomerular arterioles and enhanced afferent arteriolar Ang II contractions. The latter were blunted by incubation with H2O2. The mRNAs of renal microvascular AT1R and AT2R remained unaffected by captopril. Ang II-infused mice showed increased blood pressure and reduced afferent arteriolar Ang II responses. Administration of captopril to the Ang II-infused mice normalized blood pressure, but not arteriolar Ang II responses. We conclude that inhibition of ACE1 enhances renal microvascular reactivity to Ang II and may enhance important inflammatory pathways.


Subject(s)
Angiotensin II/pharmacology , Animals , Arterioles/metabolism , Captopril/pharmacology , Hydrogen Peroxide/pharmacology , Kidney , Mice
3.
Organ Transplantation ; (6): 317-2022.
Article in Chinese | WPRIM | ID: wpr-923576

ABSTRACT

In recent years, the science and technology of organ transplantation have developed rapidly, which has been widely applied worldwide. However, multiple challenges remain to be resolved by clinicians, such as functional damage and immune rejection of transplant organs, immune deficiency caused by extensive use of immunosuppressants, chronic allograft dysfunction and adverse reactions. This article introduced relevant key research results published in 2021, taking the function and mechanism of immune cell subsets in the process of organ transplantation rejection or immune tolerance, and the research and application of new materials and drugs in organ transplantation as the main clues. The latest research progresses on regional immune response, especially the application of tissue-resident memory T cell in organ transplantation, were briefly summarized, and the future development of transplantation immunology was prospected.

4.
Article in Chinese | WPRIM | ID: wpr-884309

ABSTRACT

Objective:To evaluate the effects of left ventricular remodeling on systolic synchronization in patients with severe preeclampsia(SPE) by full-volume imaging technology.Methods:One hundred and nine patients with SPE were randomly selected as SPE group in the First Hospital of Shanxi Medical University from December 2016 to December 2019, which were further divided into systolic synchrony(SS) group ( n=35) and systolic dyssynchrony(SD) group( n=74). And 34 healthy pregnant women during the same period were selected as normal pregnancy(NP) group. The clinical datas were collected. Parameters including left ventricular end diastolic volume(LVEDV), left ventricular end systolic volume(LVESV), left ventricular ejection fraction(LVEF), spherical index(SpI), left ventricular mass index(LVMI) and systolic dyssynchrony index(SDI) were obtained by full-volume imaging technology. The effects of left ventricular remodeling on systolic synchronization in patients with SPE were analyzed by bivariate correlation, multiple linear stepwise regression analysis and binary Logistic regression analysis, respectively. Results:①Bivariate correlation analysis showed that LVEDV, LVESV, SpI and LVMI were positively correlated with SDI( r=0.335, 0.361, 0.635, 0.680; all P<0.01). ②After adjustment for age, body mass index, systolic blood pressure, course of hypertension, antihypertensive and antispasmodic treatments, gestational diabetes mellitus, subclinical hypothyroidism, LVEF, multiple linear regression analysis showed that SpI and LVMI were independent predictors of SDI (β=0.228, 0.319; all P<0.01). ③Binary Logistic regression analysis showed that SpI and LVMI were independently correlated with left ventricular systolic dyssynchrony [ OR(95% CI)=1.288(1.039-1.598), 1.102(1.019-1.192); all P<0.05]. Conclusions:Left ventricular remodeling in patients with SPE leads to the decrease of left ventricular systolic synchronization, which can reflect subclinical myocardial dysfunction early. Full volume imaging technology can accurately evaluate left ventricular systolic synchronization in patients with SPE.

5.
Acta Physiologica Sinica ; (6): 347-360, 2020.
Article in Chinese | WPRIM | ID: wpr-827053

ABSTRACT

Interactions among the nervous, the endocrine and the immune systems enable the gut to respond to the dietary products, pathogens and microbiota, which maintains the homeostasis of the body. However, dysbiosis may induce or aggravate the gastrointestinal (GI) and extra-GI diseases through changing the activities of enteric nervous system (ENS), enteroendocrine cells and enteric immune cells. Here we review recent advances in the understandings on how intestinal flora may impact the enteric neuro-endocrine-immune system in the gut, thereby contributing to the regulation of pathophysiological processes.


Subject(s)
Enteric Nervous System , Gastrointestinal Diseases , Gastrointestinal Microbiome , Humans , Immune System
6.
Chinese Journal of Geriatrics ; (12): 287-290, 2020.
Article in Chinese | WPRIM | ID: wpr-869382

ABSTRACT

Objective:To investigate the association of serum protein kinase Cε(PKCε)activity with insulin resistance in elderly patients with concurrent type 2 diabetes mellitus(T2DM)and non-alcoholic fatty liver disease(NAFLD).Methods:Clinical data of 229 patients with T2DM admitted to the Department of Geriatric Endocrinology of the Second Affiliated Hospital of Xi'an Jiaotong University from October 2017 to October 2018 were retrospectively analyzed.Patients were divided into the T2DM group(T2DM patients without NAFLD, n=112)and the T2DM+ NAFLD group(T2DM patients with NAFLD, n=117). Healthy elderly subjects who underwent physical examination during the same period served as the control group(n=110). Clinical data and laboratory results were compared between the three groups.Serum PKCεactivity was measured by enzyme-linked immunosorbent assay, and the correlation between serum PKCεactivity and insulin resistance was analyzed statistically.Results:Serum PKCεactivity and HOMA-IR were higher in the T2DM+ NAFLD group than in the T2DM group and the control group[PKCε: (195.5±62.1) μg/L vs.(188.7±61.2 )μg/L and (89.1±20.2 )μg/L, F=9.76, P=0.010; HOMA-IR: 12.5±7.9 vs. 14.1±5.7 and 5.8±4.1, F=10.21, P=0.010]. Pearson correlation analysis revealed that serum PKCεactivity, HOMA-IR and TG were positively correlated with T2DM+ NAFLD( r=0.339, P=0.01; r=0.305, P=0.01; r=0.329, P=0.01)and that serum PKCεactivity was positively correlated with HOMA-IR and triglyceride( r=0.339, 0.305 and 0.329, P=0.01). Logistic regression analysis showed that serum PKCε activity, HOMA-IR and triglycerides were risk factors in the T2DM+ NAFLD group, and the regression coefficients were 0.849, 0.022 and 0.710, respectively.Increased serum PKCεactivity was an independent influencing factor for T2DM+ NAFLD. Conclusions:Elevated serum PKCεactivity is positively correlated with insulin resistance in elderly T2DM patients with NAFLD.Reducing serum PKCεactivity and improving insulin sensitivity will help to delay or reverse the development of T2DM and NAFLD.

7.
Article in Chinese | WPRIM | ID: wpr-846600

ABSTRACT

Objective: The extraction process optimization method of chemometrics combined with information entropy weight was established and applied to the water extraction process optimization of Banxia Baizhu Tianma Decoction (BBTD), in order to fully ensure the effectiveness and quality consistency of classical prescription. Methods: Taking BBTD as the model drug, the fingerprint was established by HPLC method. The common peak area was analyzed by principal component analysis (PCA), the total factor score of PCA, the similarity of fingerprint and the yield of dry paste were used as evaluation indexes. L9(34) orthogonal design was used to investigate the effects of adding water, soaking time and boiling time on the extraction effect, and the Objective: weight of each index was determined by information entropy weighting method. The technological parameters of water extraction were optimized. Results: Twenty-six common peaks and seven compounds were identified by the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine. According to the results of comprehensive score, the optimum extraction process of the preparation was determined to be 12 times the amount of water, decoction twice, 1 hour each time. The average comprehensive score of the three batches of verification was 0.418 0 with RSD of 3.32%. Conclusion: The optimized process has high extraction rate, good stability and repeatability, and is suitable for the industrial production of BBTD.

8.
International Eye Science ; (12): 1418-1421, 2020.
Article in Chinese | WPRIM | ID: wpr-822971

ABSTRACT

@#AIM: To evaluate the difference and consistency of pupillary offset measured by the Pentacam anterior segment analyzer and Keratron Scout corneal topographer.<p>METHODS: Three hundred and eleven patients(604 eyes)who underwent excimer laser in situ keratomileusis from November 2017 to February 2018 were randomly selected. Before surgery, the pupillary offset values were measured by Pentacam and Keratron, and the differences and consistency of the values between the two instruments were compared.<p>RESULTS: The pupillary offset values of the right eyes, left eyes, and both eyes between Pentacam and Keratron were statistically different(<i>P</i><0.05). There were no significant statistical differences in the offset orientations of right eyes, left eyes and both eyes between the two instruments(<i>P</i>>0.05). The 95% consistent line(<i>LoA</i>)of offset value and offset orientation in the right eyes, left eyes and both eyes between the two instruments were -0.11-0.19mm and -157.01°-135.35°, -0.12-0.18mm and -150.16°-158.22°, -0.11-0.19mm and -154.30°-147.10°, respectively. <p>CONCLUSION: The pupillary offset measured by the Pentacam was smaller than that measured by the Keratron, but the difference was within the clinically acceptable range. The accurate pupillary offset can be obtained, and be the mutual reference, correction and supplement in the both instruments.

9.
Chinese Journal of Hematology ; (12): 650-655, 2019.
Article in Chinese | WPRIM | ID: wpr-805798

ABSTRACT

Objective@#To investigate the safety and efficacy of allogeneic CAR-T cells in the treatment of relapsed/refractory multiple myeloma (RRMM) .@*Methods@#CAR-T cells were prepared from peripheral blood lymphocytes of HLA mismatch healthy donors. Median age was 55 (48-60) . Allogeneic cells were derived from 3 HLA haploidentical donors and 1 HLA completely mismatch unrelated donor. Four patients with RRMM were conditioned with FC regimen followed by CAR-T cell transfusion. They were infused into CART-19 (1×107/kg on day 0) and (4.0-6.8) ×107/kg CART-BCMA cells as split-dose infusions (40% on day 1 and 60% on day 2) . The adverse reactions and clinical efficacy were observed during follow-up after infusion, and the amplification and duration of CAR-T cells in vivo were monitored by PCR technique.@*Results@#CAR-T cells were successfully infused in 3 of the 4 RRMM patients according to the study plan, and the infusion in one patient was delayed by 1 day due to high fever and elevated creatinine levels on day 3. The side effects included hematological and non-hematological toxicity, grade 3 hematological toxicity in 2 patients, grade 3 CRS in 1 one, grade 1 CRES in 1 one, prolonged APTT in 3 ones, tumor lysis syndrome in 1 one, mixed chimerism detected STR and clinical GVHD manifestation in 1 one. According to the efficacy criterias of IMWG, 2 patients acquired PR, 1 MR, and 1 SD respectively. Progression-free survival was 4 (3-5) weeks and overall survival was 63 (3-81) weeks. CAR T cells were amplified 2.2 (2-14) times in the patients with a median survival time of 10 (8-36) days.@*Conclusions@#Small sample studies suggested that GVHD may be present in the treatment of RRMM with allogeneic CAR-T cells. There were early clinical transient events after transfusion. Low amplification and short duration of CAR-T cells in vivo may be the main factors affecting the efficacy.

10.
Article in Chinese | WPRIM | ID: wpr-802440

ABSTRACT

Objective: To study on the molecular mechanism of anti-platelet aggregation and anti-thrombosis of Sparganii Rhizoma. Method: Based on the data information and analysis functions of traditional Chinese medicine(TCM) database,TCM composition database and target database in TCM platform for integration of pharmacology,information of chemical compositions in Sparganii Rhizoma was retrieved,interaction network between potential targets of Sparganii Rhizoma and disease targets was built,and the key targets were enriched and calculated,the gene functions and pathways were analyzed,multidimensional relationship network of " herbal medicines-chemical components-key targets-key pathways" was built. Result: Active ingredients of Sparganii Rhizoma mainly included flavonoids and phenolic acids,such as mountain kaempferol,sanleng acid,linoleic acid,etc.Anti-thrombosis involved 202 key targets,including protein kinase Cδ(PRKCD),glucose kinase(GCK),(PRKAA2),adenosine ribonucleotide activated protein kinase α-2 catalytic subunit,etc;and it was related to the endocrine system,circulatory system,neurodegenerative diseases and other related biological processes and signal pathways.Anti-platelet aggregation involved 136 key targets,including PRKCD,cytochrome C oxidase protein 7C(COX7C),GCK,etc;and it was involved in Parkinson's disease,circulatory system,neurodegenerative diseases and other related biological processes and signal pathways. Conclusion: Sparganii Rhizoma regulates vascular endothelial cell adhesion molecule expression and platelet mitochondrial function mediated apoptosis of platelets mainly through regulating neurotransmitter activity in the central nervous system,in order to exert antithrombotic effect and anti-platelet aggregation effect.

11.
Article in Chinese | WPRIM | ID: wpr-798502

ABSTRACT

Objective:To explore the molecular mechanism of Banxia Baizhu Tianma Tang in treating hypertension. Method:Based on an internet-based computation platform for integrated pharmacology of traditional Chinese medicine(TCM),TCM prescription database,TCM database,TCM component database and disease/symptoms target database,information on the chemical compositions contained in TCM was retrieved,an interaction network between potential targets and disease targets of Banxia Baizhu Tianma Tang was built,then core target was enriched and calculated,gene function and pathway analysis was carried out,the multi-dimensional relationship network of "Chinese herbs-ingredients-critical targets-key pathways" of Banxia Baizhu Tianma Tang was further constructed. Result:A total of 90 active ingredients in Banxia Baizhu Tianma Tang were obtained,including alkaloids,nucleosides,flavonoids,saponins,organic acids and other ingredients;they involved 287 core targets,including 13 direct targets,such as adenylate cyclase,G protein coupled with β1 receptor,glucose kinase,etc;they also involved nervous system,endocrine system,circulatory system,estrogen signaling pathway,chemokine signaling pathway and other related biological processes and signaling pathways. Conclusion:Banxia Baizhu Tianma Tang can regulate neurotransmitter concentration and activity abnormalities,improve the protective effect of vascular endothelial cells by improving insulin resistance,regulating the production of inflammatory factors,and inhibiting inflammatory reactions,thereby exerting pharmacological effects on the treatment of hypertension.This study can provide a scientific basis for comprehensive interpretation of mechanism of Banxia Baizhu Tianma Tang.

12.
Article in Chinese | WPRIM | ID: wpr-851241

ABSTRACT

Objective To establish a combinative method based on HPLC fingerprint, multi-component assay, and pattern recognition for quality evaluation of Sparganii Rhizoma. Methods The similarity analyzed with “Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A”, Nine kinds of components were identified by comparison with reference substances and their content in samples were determined and hierarchical clustering analysis (HCA) and principal component analysis (PCA) were performed by SPSS 20.0. Results There were 12 common peaks, and the similarity degrees of 12 batches of samples were more than 0.800, which showed that all the samples from different origins were of good consistency. Nine of them were identified as 5-hydroxymethylfurfural, vanillic acid, ferulic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, vanillin, protocatechuic acid, p-coumaric acid, and isoferulic acid; The samples were divided into four clusters by HCA. The result of PCA showed, the quality of samples could be evaluated basically. According to the composite score, the quality of Sparganii Rhizoma from different origins, Henan Xinxiang, Jiangsu Zhenjiang, Hebei Cangzhou, Hunan Yueyang, Zhejiang Jinhua, and Henan Zhengzhou were better than others. There was a correlation between the scores of the main components of the 12 batches of decoction pieces and the results of the content determination. Conclusion The method is simple, reproducible, and reliable, it can be used for quality control and evaluation of Sparganii Rhizoma from different origins.

13.
Article in Chinese | WPRIM | ID: wpr-850806

ABSTRACT

Objective: To identify the quality markers (Q-marker) for Baizhu Tianma Decoction from different batches based on multivariate statistical analysis. Methods: The fingerprint data of 18 batches of Banxia Baizhu Tianma Decoction were processed by the Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine (2012A Edition). SPSS 20.0 was used for cluster analysis and principal component analysis (PCA). According to PCA results, cluster analysis was carried out to screen out the markers that might affect the quality difference of Banxia Baizhu Tianma Decoction and determine its content in the samples. Results: A total of 12 chromatographic peaks were selected as common peaks of fingerprint, and the similarity of 18 batches of samples was greater than 0.900, which indicated that each batch had good consistency. Eighteen batches of samples were clustered into three groups by cluster analysis. Five marker peaks were screened out from PCA results. Two of them were identified as gastrodin and naringin. The five marker peaks were further clustered and the results were basically the same as before. There was a certain correlation between the comprehensive score of principal components and the determination results of gastrodin and naringin in 18 batches of samples. Conclusion: Through multivariate statistical analysis, the Q-markers that may affect the difference of Banxia Baizhu Tianma Decoction were better identified, which can lay the foundation for the comprehensive quality control of Banxia Baizhu Tianma Decoction and its compound preparations, and provide reference for the further study of the correlation between the different components and pharmacodynamics of Banxia Baizhu Tianma Decoction.

14.
Article in Chinese | WPRIM | ID: wpr-815798

ABSTRACT

Respiratory tract infection;Multiplex PCR;Children@#Abstract:Objective The aim of the study was to evaluate a multiplex molecular real ⁃ time assay for the diagnosis of respiratory viruses of childhood acute respiratory virus infection,and analyze the symptoms associated with the pathogens which were detected from those samples. Methods From August 2018 to August 2019,a sample of 275 swallow swabs from children with acute respiratory infections in Chengdu Children′s Specialist Hospital was collected and tested using the RespiFinder®SMART 22 test kit. The kit is based on multiplex PCR technology and can detect and distinguish 22 pathogens related to respiratory infections at one time. Results Of the 275 samples,245(89.1%)detected positive viral infections, of which 160 were single virus infections,59 were multiple viral infections,17 were positive for Mycoplasma pneumoniae, and 9 were positive for Chlamydia pneumoniae. The most common pathogens were rhinovirus 77 cases(28.0%),adenovirus 45 cases(16.4%)and respiratory syncytial virus 34 cases(12.4%). Influenza virus was detected in 28(10.2%)samples, parainfluenza in 25 cases(9.1%),coronavirus in 15 cases(5.5%),Boca virus in 12 cases(4.4%),and human metapneumovirus in 9 cases (3.3%). Rhinovirus (28.0%) was the most common pathogen in upper respiratory tract infections,while respiratory syncytial virus(12.4%)was the most common pathogen in lower respiratory tract infections. It has been found that multiple viral infections in children were associated with severe respiratory symptoms in clinical manifestations. Conclusion Compared with ordinary PCR detection,the multiplex PCR could detect multiple pathogens at one time in experiment,which greatly shortens the detection time and had important clinical significance for rapid diagnosis of multiple respiratory pathogens. Thus,it could be useful for detecting several viruses causing respiratory tract disorders.

15.
Article in Chinese | WPRIM | ID: wpr-776034

ABSTRACT

Objective To investigate the effects of different inflammatory factors on hepatocyte kinase receptor(Eph)and ligand(ephrin)in human periodontal ligament fibroblasts(hPDLFs).Methods hPDLFs were stimulated with either 10 ng/ml tumor necrosis factor-α(TNF-α)or 10 ng/ml interleukin(IL)-1β,and then the expressions of Eph and ephrin at both mRNA and protein levels were determined at 0,1,2,6,12,and 24 hours.Results The levels of Eph receptors and ephrin ligand changed in a time-dependent manner in human periodontal ligament fibroblasts after treatment with TNF-α or IL-1β. The expression of ephrinA2 significantly increased in both groups within 24 hours(all <0.05). In the TNF-α group,the mRNA expression of ephrinA2 significantly increased at 1 h and was significant higher that in the IL-1β group at 24 h(<0.05). EphB4 showed a time-dependent decline after a short period of high expression.Conclusions Both TNF-α and IL-1β can cause changes in the expressions of Eph receptors and ephrin ligands in hPDLFs. The changes induced by both are consistent,although the effect of TNF-α is more pronounced.


Subject(s)
Cells, Cultured , Ephrins , Metabolism , Fibroblasts , Humans , Interleukin-1beta , Pharmacology , Ligands , Periodontal Ligament , Cell Biology , Receptors, Eph Family , Metabolism , Tumor Necrosis Factor-alpha , Pharmacology
16.
Article in Chinese | WPRIM | ID: wpr-775908

ABSTRACT

' acupuncture and moxibustion physicians have a lot of talented people. Their academic theories promote the development of moxibustion theory. - used acupuncture and moxibustion together in clinic, and emphasized reinforcing and reducing method. Moxibustion was performed with unique matching acupoint according to different cases. proposed the theory "moxibustion can cure sores, and has reinforcing and reducing method"scars block the movement of and do not use moxibustion when there is no disease". - elaborated on the theory of moxibustion, which involved a wide range of ideas and advocated the idea of combining acupuncture with drugs. put forward the theory "moxibustion is mostly used at acupoints on the back, and can cure multiple syndromes" "focus on the use of miraculous acupoints for the treatment of emergency diseases" "moxibustion must be treated with enough moxibustion to cure the disease". - thonght that only by carefully identifying the types of diseases and using corresponding acupoints could have a very good curative effect; moxibustion had indications and contraindications, so be careful when used it; moxibustion was divided into and , also divided into reinforcing and reducing methods; sores were suitable for moxibustion and heat syndrome could also be used by moxibustion. He also believed that moxibustion was as important as acupuncture with the complementary relationship. Hence, the valuable significance of moxibustion in clinical practice is explored through the collection of the academic thoughts of -, , -, and -.


Subject(s)
Acupuncture Therapy , China , Humans , Moxibustion , Physicians
17.
Article in Chinese | WPRIM | ID: wpr-775855

ABSTRACT

OBJECTIVE@#To observe the effects of electroacupuncture (EA) at "Jiaji" (EX-B 2) points combined with nerve mobilization on protein and mRNA expression of RhoA in rabbits with sciatic nerve injury, and to provide theoretical basis for the treatment of peripheral nerve injury by EA at "Jiaji" (EX-B 2) points combined with nerve mobilization.@*METHODS@#A total of 180 New Zealand rabbits were randomly divided into a normal control group, a model control group, a nerve mobilization group, an EA group, an EA plus nerve mobilization group, 36 rabbits in each group. Each group was further divided into a 1-week subgroup, 2-week subgroup and 4-week subgroup, 12 rabbits in each subgroup. The sciatic nerve injury model was made by clamping method. The rabbits in the normal control group did not receive any intervention. The rabbits in the model control group was normally fed after operation. The rabbits in the nerve mobilization group were treated with nerve mobilization; the manipulation lasted for 1 s and relaxed for 5 s, 10 times per day, 6 days per week. The rabbits in the EA group were treated with EA at "Jiaji" (EX-B 2) points (L-L), once a day, 30 min each time, 6 times per week. The rabbits in the EA plus nerve mobilization group were treated with EA at "Jiaji" (EX-B 2) points, followed by nerve mobilization. The function of sciatic nerve on the injured side was evaluated by toe tension reflex and modified Tarlov score; the tissues of corresponding segments of spinal cord L-L and sciatic nerve were taken; the expression of RhoA gene was detected by real-time PCR and the expression of RhoA protein was detected by Western Blot.@*RESULTS@#① Toe tension reflex and modified Tarlov score: at 1, 2 and 4 weeks, the scores in the model control group were lower than those in the normal control group (all 0.05); at 2 weeks, the expression in the nerve mobilization group was higher than that in the EA group (all <0.01); at 4 weeks, the expression in the nerve mobilization group was lower than that in the EA group (all <0.01).@*CONCLUSION@#The nerve mobilization and EA at "Jiaji" (EX-B 2) points could both promote the repair of injured sciatic nerve, which may be related to the down-regulation of RhoA expression, and the combination of the two methods has better effects.


Subject(s)
Acupuncture Points , Animals , Chlorophenols , Electroacupuncture , Peripheral Nerve Injuries , RNA, Messenger , Metabolism , Rabbits , Sciatic Nerve , Wounds and Injuries , rhoA GTP-Binding Protein
18.
Article in Chinese | WPRIM | ID: wpr-773118

ABSTRACT

The Shenghua Decoction recorded in Fu Qing Zhu's Gynaecology,is a commonly used postpartum prescription,widely used in treating postpartum and gynecological diseases. However,its mechanism of action in treating lower abdominal pain remains unclear. In this paper,network pharmacology was used to explore the mechanism of Shenghua Decoction in the treatment of lower abdomen pain,so as to provide data support for better clinical application of Shenghua Decoction. The drug targets of lower abdominal pain and Shenghua Decoction were retrieved in SymMap. String and Cytoscape were adopted for enrichment analysis to construct the disease-drug-target biological network. Relevant gene search results showed that there were 400 targets in Shenghua Decoction,11 of which coincided with the disease genes of lower abdomen pain. In String analysis,18 gene interactions were obtained. Gene modularizationbased analysis results indicated that one module containing six genes was obtained after modularization processing. Furthermore,there were 170 enrichment results of biological process,2 enrichment results of molecular function and 30 enrichment results of KEGG pathways in String enrichment analysis. Shenghua Decoction may play a role in treating lower abdomen pain through neuro-endocrine-immune,metabolism and other means. Its mechanism may be achieved by accelerating the repair and growth of endometrial tissue cells,improving microcirculation,promoting endometrial cell renewal and inflammation subsidence,and accelerating uterine involution; at the same time,it can regulate the autoimmunity,regulate and control the function of some natural immune cells in the process of antiinfection by using signaling pathway,supplement the vital energy,and induce elimination of pathogens from the body,thereby achieving the effect of treating lower abdomen pain.


Subject(s)
Abdominal Pain , Drug Therapy , Drugs, Chinese Herbal , Pharmacology , Female , Gynecology , Humans , Signal Transduction
19.
Chinese Journal of Biotechnology ; (12): 1901-1913, 2019.
Article in Chinese | WPRIM | ID: wpr-771744

ABSTRACT

To quickly and efficiently understand the intracellular metabolic characteristics of industrial microorganisms, and to find potential metabolic engineering targets, genome-scale metabolic network models (GSMMs) as a systems biology tool, are attracting more and more attention. We review here the 20-year history of metabolic network model, analyze the research status and development of GSMMs, summarize the methods for model construction and analysis, and emphasize the applications of metabolic network model for analyzing intracellular metabolic activity of microorganisms from cellular phenotypes, and metabolic engineering. Furthermore, we indicate future development trend of metabolic network model.


Subject(s)
Industrial Microbiology , Metabolic Engineering , Metabolic Networks and Pathways , Genetics , Models, Biological , Systems Biology
20.
Acta Physiologica Sinica ; (6): 883-893, 2019.
Article in Chinese | WPRIM | ID: wpr-781386

ABSTRACT

In this study, we improved the culture method of mouse hippocampal primary microglia to obtain hippocampal ramified microglia with high activity and purity, which were resemble to the resting status of normal microglia in healthy brain in vivo. Hippocampal tissue was excised from 2-4-week-old SPF C57BL/6J mice and cut into pieces after PBS perfusion, and then manually dissociated into the single-cell suspension by using Miltenyi Biotec's Adult Brain Dissociation Kit. The tissue fragments such as myelin in the supernatant were removed by debris removal solution in the kit. The cell suspension was incubated with CD11b immunomagnetic beads for 15 min at 4 °C. To obtain high-purity microglia, we used two consecutive cell-sorting steps by magnetic activated cell sorting (MACS). After centrifugation, the cells were resuspended and seeded in a 24-well culture plate. The primary microglia were cultured with complete medium (CM) or TIC medium (a serum-free medium with TGF-β, IL-34 and cholesterol as the main nutritional components) for 4 days, and then were used for further experiments. The results showed that: (1) The cell viability was (56.03 ± 2.10)% by manual dissociation of hippocampus; (2) Compared with immunopanning, two-step MACS sorting allowed for efficient enrichment of microglia with higher purity of (86.20 ± 0.68)%; (3) After being incubated in TIC medium for 4 d, microglia exhibited branching, quiescent morphology; (4) The results from qRT-PCR assay showed that the levels of TNF-α, IL-1β and CCL2 mRNA in TIC cultured-microglia were similar to freshly isolated microglia, while those were much higher in CM cultured-microglia after incubation for 4 d and 7 d (P < 0.05). Taken together, compared to the conventional approaches, this modified protocol of mouse hippocampal primary microglia culture by using MACS and TIC medium enables the increased yield and purity of microglia in the quiescent state, which is similar to normal ramified microglia in healthy brain in vivo.


Subject(s)
Animals , Cell Culture Techniques , Methods , Cell Separation , Methods , Cells, Cultured , Hippocampus , Magnetics , Mice , Mice, Inbred C57BL , Microglia , Cell Biology
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