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1.
Article in English | WPRIM | ID: wpr-762458

ABSTRACT

BACKGROUND@#Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase (gyrA) and topoisomerase IV (parC) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect mutations in the gyrA and parC QRDRs associated with FQ resistance in A. baumannii.@*METHODS@#Based on the conserved sequences of A. baumannii gyrA and parC, two primer sets were designed for mismatched PCR-RFLP to detect mutations in gyrA (codons 83 and 87) and parC (codons 80 and 84) by introducing an artificial restriction enzyme cleavage site into the PCR products. This assay was evaluated using 58 A. baumannii strains and 37 other Acinetobacter strains that have been identified by RNA polymerase β-subunit gene sequence analysis.@*RESULTS@#PCR amplification of gyrA and parC was successful for all A. baumannii strains. In 11 FQ -susceptible strains, the gyrA and parC PCR products were digested by the selected restriction enzymes at the site containing gyrA (codons 83 and 87) and parC (codons 80 and 84). PCR products from 47 FQ-resistant strains containing mutations in gyrA and parC were not digested by the restriction enzymes at the site containing the mutation. As for the non-baumannii Acinetobacter strains, although amplification products for gyrA were obtained for 28 strains, no parC amplification product was obtained for any strain.@*CONCLUSIONS@#This assay specifically amplified gyrA and parC from A. baumannii and detected A. baumannii gyrA and parC mutations with FQ resistance.

3.
Article in Japanese | WPRIM | ID: wpr-371156

ABSTRACT

The actual measurement of the body surface area generally take much trouble. And the existance of some interrelation among the height, weight and the others now lead to the calculation of the body surface area using a way of multipling these factors by a constant coefficient. After much investigation about a variety of calculation formula which have desingned by now we reached a conculusion that a from A=K<SUB>1</SUB>X+K<SUB>2</SUB>Y+Q (Howland-Dana from) is more desirable than the others, above all by applying X for the height (Hcm) and Y for the weight (Wg) the most desirable approximation can be got. From this point of view we achieved a new calculation formula for Japanease adult man and woman as follows. A=0.0901W+62.49H+266 for man and A=0.1309W+ 37.33H + 1799 for woman come as the result.

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