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1.
Article in Chinese | WPRIM | ID: wpr-662428

ABSTRACT

Objective To explore the application of A-V impulse foot pump in patients with lumbar spine surgery to prevent lower extremity deep venous thrombosis. Methods A total of 139 patients with spinal lesions who took spinal surgery treatment from April 2015 to March 2016 were divided into experimental group (69 cases) and control group (70 cases) by random digits table method. Control group was given normal nursing instruction, and the person who had negative results of B ultrasonic examination in experimental group was accepted physical therapy with A-V impulse foot pump. The patients got secondary ultrasound or CT examination who had the deep venous thrombosis symptoms and/or highly suspected deep venous thrombosis in order to further confirmed, then calculated the incidence rate of two groups with deep venous thrombosis. Results There were 4 cases of 139 patients who got deep venous thrombosis in 2-6 days of postoperative, 1 case in experimental group, 3 case in control group, compared with control group, patients who used A-V impulse foot pump had lower risk of deep venous thrombosis, but there was no significant difference (P>0.05). The operation time, body mass index were (5.00 ± 0.82) h , (30.54 ± 3.93) kg/m2 in patients with deep venous thrombosis and (3.16 ± 0.66) h, (24.90 ± 2.62) kg/m2 in patients without deep venous thrombosis, and there were significant differences(t=5.44, 4.19, P<0.01). Conclusions The application of A-V impulse foot pump has positive significance to prevent the happening of deep venous thrombosis for patients who took spinal surgery, and it is worth to be popularized and applied in clinic.

2.
Article in Chinese | WPRIM | ID: wpr-660021

ABSTRACT

Objective To explore the application of A-V impulse foot pump in patients with lumbar spine surgery to prevent lower extremity deep venous thrombosis. Methods A total of 139 patients with spinal lesions who took spinal surgery treatment from April 2015 to March 2016 were divided into experimental group (69 cases) and control group (70 cases) by random digits table method. Control group was given normal nursing instruction, and the person who had negative results of B ultrasonic examination in experimental group was accepted physical therapy with A-V impulse foot pump. The patients got secondary ultrasound or CT examination who had the deep venous thrombosis symptoms and/or highly suspected deep venous thrombosis in order to further confirmed, then calculated the incidence rate of two groups with deep venous thrombosis. Results There were 4 cases of 139 patients who got deep venous thrombosis in 2-6 days of postoperative, 1 case in experimental group, 3 case in control group, compared with control group, patients who used A-V impulse foot pump had lower risk of deep venous thrombosis, but there was no significant difference (P>0.05). The operation time, body mass index were (5.00 ± 0.82) h , (30.54 ± 3.93) kg/m2 in patients with deep venous thrombosis and (3.16 ± 0.66) h, (24.90 ± 2.62) kg/m2 in patients without deep venous thrombosis, and there were significant differences(t=5.44, 4.19, P<0.01). Conclusions The application of A-V impulse foot pump has positive significance to prevent the happening of deep venous thrombosis for patients who took spinal surgery, and it is worth to be popularized and applied in clinic.

3.
Article in Chinese | WPRIM | ID: wpr-494403

ABSTRACT

Objective: To use autophagy inhibitors combined with radiation to treat the oral squamous cell carcinoma CAL-27 and KB cells,and to explore the influence of autophagy in the oral cancer radiation sensitivity and its mechanisms. Methods:The human oral squamous cell carcinoma CAL-27 and KB cells were divided into control group,CQ group,3-MA group,IR group,CQ+IR group,and 3-MA+IR group. The survival rate was detected by MTT method and the autophagy of CAL-27 cells was observed by immunofluorescence method and laser scanning confocal microscope.The expression levels of LC3 and beclin-1 were detected by Western blotting method. The apoptotic rate was determined by flow cytometry with Annexin Ⅴ/PI doulde staining. Results:Compared with IR group,the survival rates in 3-MA + IR and CQ+ IR groups were signifcantly decreased (P < 0.05 ).The autophagy levels of cells in IR group were significantly higher than those in control group, CQ group, 3-MA group,CQ+IR group,and 3-MA+IR group (P <0.05).The expression levels of LC3 and beclin-1 proteins in IR group were significantly higher than those in control group,CQ+ IR group,and 3-MA+ IR group (P < 0.05). The apoptotic rates in IR,3-MA+ IR,and CQ+ IR groups were markedly higher than those in control group. Compared with IR group,the apoptotic rates in CQ+IR and 3-MA+IR groups were significantly increased (P <0.05).Conclusion:Radiotherapy can induce the increase of autophagy level of oral squamous cell carcinoma cells. Inhibiors of autophagy can increase the radio-sensitivity of oral squamous cell carcinoma cells by inhibiting the proliferation and inducing the apoptosis.

4.
Article in Chinese | WPRIM | ID: wpr-504802

ABSTRACT

Objective:To investigate the effect of poly (lactic acid)(PLA)electrospun membranes loaded with cisplatin and chloroquine on the oral squamous cell carcinoma CAL-27 cells,and to explore the method to prevent the recurrence of oral cancer.Methods: The DDP/PLA membranes, CQ/DDP/CQ/PLA membranes and CQ/DDP/PLA membranes were prepared by electrospinning.Then the micro morphology of three kinds of membranes were observed by scanning electron microscope (SEM);the degradation rate of PLA membrane was measuredby UV spectrophotometric.The LC3-Ⅱ expression level in CAL-27 cells was detected by laser scanning confocal microscope.The survival rate of CAL-27 cells was detected by MTT method.Results:The SEM results showed that the nanofibers of DDP/PLA,CQ/DDP/PLA and CQ/DDP/CQ/PLA membranes were continuous and smooth with uniform diameters.The degrated time of membranes was about 21 d.The MTT result showed that compared with control group,at first,the effects of cell killing of DDP/PLA membranes,CQ/DDP/CQ/PLA membranes and CQ/DDP/PLA membranes were not obvious;as the extension of time,the survival rates of CAL-27 cells in DDP/PLA membranes group,CQ/DDP/CQ/PLA membranes group and CQ/DDP/PLA membranes group were decreased (P <0.05).The immunofluorescence results showed that the fluorescence intensity of LC3-Ⅱ in CQ/DDP/CQ/PLA membranes group and CQ/DDP/PLA membranes group were lower than that in DDP/PLA membranes group.Conclusion:CQ/DDP/PLA membranes with sustained-release effect can increase the sensitivity of CAL-27 cells to DDP and enchance the killer effect of DDP on the CAL-27 cells.

5.
Article in Chinese | WPRIM | ID: wpr-326066

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the association between occupational stress, social support, and occupational unintentional injuries.</p><p><b>METHODS</b>A 1:1 matched case-control study was conducted in 151 cases of occupational unintentional injuries who were admitted to 6 occupational injury-admitted hospitals in Zhongshan City from October 2013 to December 2013 and 151 matched controls without unintentional injuries in the last year who had matched age, sex, and occupation. Their demographic characteristics, occupational stress (by the effort-reward imbalance questionnaire), and social support were investigated with a structured questionnaire.</p><p><b>RESULTS</b>Analysis of the data showed that there were significant differences in the score of each dimension of occupational stress, the ratio of effort to reward, and the score of superior support between the case group and the control group (P < 0.05). The Cox regression analysis results showed that more extrinsic efforts (OR = 1.47, 95%CI = 1.20∼1.80) and over commitment (OR = 1.30, 95%CI = 1.08∼1.55) were the risk factors for occupational unintentional injuries, while more superior supports (OR = 0.64, 95%CI = 0.48∼0.84) and higher earnings (>3 000 yuan each month) (OR = 0.67, 95%CI = 0.54∼0.84) were protective factors.</p><p><b>CONCLUSION</b>Occupational stress and social support have an influence on the occurrence of occupational injuries.</p>


Subject(s)
Accidents, Occupational , Case-Control Studies , China , Humans , Occupational Diseases , Epidemiology , Occupational Injuries , Epidemiology , Reward , Risk Factors , Social Support , Stress, Psychological , Epidemiology , Surveys and Questionnaires
6.
China Pharmacist ; (12): 1711-1714, 2015.
Article in Chinese | WPRIM | ID: wpr-670068

ABSTRACT

Objective:To establish an HPLC method for the determination of related substances in enalapril maleate dispersible tablets.Methods:AKromasil100-SC8(250mm×4.6mm,5μm)columnwasused. ThemobilephaseAwas0.01mol·L-1potassi-um dihydrogen phosphate (adjusting pH to 2. 2 with phosphoric acid) and mobile phase B was acetonitrile with gradient elution,and the flow rate was 1. 0 ml· min-1 . The detection wavelength was 215nm,the column temperature was 40℃,and the injection volume was 20 μl. Results:The linear range of enalaprilat,enalapril maleate and eanlapril diketopiperazine was 14.64-43.92 μg·ml-1(r =0. 999 8), 9. 29-27. 87 μg·ml-1(r=0. 999 1)and 10. 80-32. 40 μg·ml-1(r=0. 999 5), respectively. Enalaprilat was not detec-ted out and enalapril diketone was 0. 2% using the method described in Chinese pharmacopeia, and the content of enalaprilat was 0. 1% and that of enalapril diketone was 0. 2% using the gradient elution. The related substances and enalapril maleate could be sepa-rated well. Conclusion:The method is sensitive, accurate and highly specific in the quality evaluation and control.

7.
China Pharmacy ; (12): 2567-2568,2569, 2015.
Article in Chinese | WPRIM | ID: wpr-605112

ABSTRACT

OBJECTIVE:To establish a method for the content determination of Aciclovir cream. METHODS:Centrifugal parti-tion chromatography(CPC)was conducted. The solvent system was hexane-acetonitrile-water(2∶1∶1,V/V/V),the injection vehicle was an aqueous solution of 5%Tween 80 and the volume was 1.0 ml;the flow rate was 5 ml/min;the wavelength was 254 nm. RE-SULTS:There was a good linear relationship between quality concentration and peak area in the range of 0.012 7-0.126 7 mg/ml (r=0.998 7). The RSD of precision,stability and reproducibility tests was all no more than 2.0% and the average recovery was 97.34%(RSD=0.90%,n=9). CONCLUSIONS:The method is with high precision and accuracy,and can be used for the content determination of principal components of Aciclovir cream.

8.
China Pharmacist ; (12): 520-522, 2015.
Article in Chinese | WPRIM | ID: wpr-669664

ABSTRACT

Objective:To establish an HPLC method for the determination of gabapentin and the related substances in gabapentin capsules. Methods:A Kromasil C8 column (250 mm × 4. 6 mm,5μm)was used. The mobile phase was methanol-0. 01 mol·L-1 po-tassium dihydrogen phosphate solution (adjusting pH to 6. 9 with 2 mol·L-1 potassium hydroxide) (40∶60). The detection wave-length was 210 nm. The column temperature was 40℃ and the injection volume was 20μl. The main component and the known impuri-ty were determined by an external standard method. The unknown impurities were determined by a self-control method. Results: The calibration curve of gabapentin was linear within the range of 2.5-20.0 mg·ml-1(r=0.999 9). The average sample recovery was 100. 74% with RSD of 0. 24%(n=5). Conclusion:The method is simple, accurate,specific and applicable in the determination of gabapentin and the related substances in gabapentin capsules.

9.
Chinese Medical Journal ; (24): 691-695, 2014.
Article in English | WPRIM | ID: wpr-317916

ABSTRACT

<p><b>BACKGROUND</b>Infertility is one of the most important and underappreciated reproductive health problems in developing countries. However, epidemiological data in the Chinese population are still sparse. The aim of the present study was to determine the current prevalence and prevalence trend of infertility in Beijing, and to identify the risk factors associated with infertility.</p><p><b>METHODS</b>This cross-sectional study was conducted in Beijing in 2012. A stratified cluster sampling method was used to select 12 448 couples of whom the female partners were born between 1955 and 1985. All subjects were interviewed face to face. Infertility was defined as the failure to achieve a clinical pregnancy after 12 months of regular unprotected sexual intercourse.</p><p><b>RESULTS</b>Of the 12 448 couples, 12 342 (99.1%) answered the questions appropriately. The prevalence of infertility was 4.2% (3.1% as primary and 1.1% as secondary infertility). An increase in the prevalence of infertility according to the age of the female partner was found: 1.3% infertility for married females born in the 1950s and 11.4% for married females born in the 1980s. The increase was found in both urban and suburban areas. In addition, a Logistic regression showed that for the female partner, higher education levels, an older age at first marriage, adverse occupational conditions, mental labor and pre-pregnancy contraception after marriage were all significantly associated with a higher risk of infertility.</p><p><b>CONCLUSION</b>The prevalence of infertility has increased significantly among couples in Beijing, possibly because of an interaction among multiple factors.</p>


Subject(s)
Adult , China , Epidemiology , Cross-Sectional Studies , Female , Humans , Infertility , Epidemiology , Male , Middle Aged , Prevalence , Risk Factors
10.
Chinese Journal of Cardiology ; (12): 510-514, 2014.
Article in Chinese | WPRIM | ID: wpr-316423

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the relationship between normal high blood pressure and carotid artery atherosclerosis.</p><p><b>METHODS</b>This epidemiological survey was performed in two communities of Shijingshan District of Beijing from 2007 to 2008. A total of 3 324 people were enrolled in the study and 2 895 people with carotid ultrasound survey results were analyzed. Blood lipids, blood pressure, hs-CRP, body mass index (BMI) and other cardiovascular risk factors were obtained. Carotid mean intima-media thickness (IMT) , maximum carotid IMT and carotid plaque were measured by ultrasound. A multiple logistic regression model was used to evaluate the relationship between normal high blood pressure and carotid artery atherosclerosis.</p><p><b>RESULTS</b>The carotid mean IMT, maximum IMT, incidence of carotid artery plaque increased in proportion to blood pressure level (all P < 0.01) . After adjusting for diabetes, hypercholesterolemia, hypertriglyceridemia, increased hs-CRP, smoking, sex, age, overweight and obesity, multiple logistic regression analysis showed that the OR values of carotid mean IMT and maximum IMT thickening, carotid plaque in high normal blood pressure group were 4.50 (95% CI: 1.04-19.49, P < 0.05), 1.73 (95% CI: 1.35-2.22, P < 0.01), 1.64 (95% CI:1.26-2.13, P < 0.01) compared to normal blood pressure group.</p><p><b>CONCLUSION</b>High normal blood pressure is related with higher risk of carotid artery atherosclerosis in this cohort.</p>


Subject(s)
Adult , Aged , Blood Pressure , Carotid Artery Diseases , Epidemiology , Pathology , Carotid Intima-Media Thickness , Female , Humans , Logistic Models , Male , Middle Aged , Risk Factors
11.
Article in Chinese | WPRIM | ID: wpr-448019

ABSTRACT

Objective To investigate the prediction value of ultrasound endometrial pattern at the day of hCG administration on clinical outcome in patients undergoing in vitro fertilization-embryo transfer (IVF-ET).Methods 679 cycles of IVF-ET conducted between January 2011 and December 2012 in Beijing Obstetrics and Gynecology Hospital were reviewed retrospectively.All cycles were divided into three groups based on the endometrial pattern measured on the hCG day:group A (n =411) with triple line endometrium,group B (n =228) with no obvious triple line endometrium,group C (n =40) with homogeneous hyperechoic endometrium.Clinical outcomes such as implantation rate,pregnancy rate and miscarriage rate were analyzed.Results Among three groups,the implantation rates were 27.05%,20.33%,19.57%,respectively (x2 =9.04,P =0.01); the pregnancy rates were 34.31%,25.00%,12.50%,respectively (x2 =12.20,P =0.00).The implantation rate and pregnancy rate in group A were higher than the other two groups (P <0.05).The miscarriage rates were 16.31%,19.30%,40.00%,respectively(P > 0.05),there were no significant difference among them.Conclusions The ultrasound endometrium pattern on the day of hCG administration has predictive value on clinical outcome in patients undergoing in vitro fertilization-embryo transfer.

12.
Article in Chinese | WPRIM | ID: wpr-499953

ABSTRACT

Objective To analyze the relationship between obesity and arterial stiffness among population of different glucose tolerance status. Methods A cross-sectional study recruited the population aged 40 years or older from ShiJingShan district in Beijing. 9080 subjects were included by measured weight, waist circumference (WC), body mass index (BMI), waist/hip ratio (WHR) and WC/height ratio (WHtR) and hemodynamic indexes and the aortic stiffness (using brachial-ankle pulse wave velocity(baPWV). They were divided into 3 groups based on the results of OGTT and diabetes history: normal glucose tolerance group ( NGT group) ,impaired glucose regulation group ( IGR group) and diabetes mellitus group ( DM group) . The association between baPWV and different obese indexes was analyzed by multi-ple linear regression. Results According to the criterion of WC, WHR and WHtR, baPWV of central obesity group was significantly higher than the normal group(P0. 05), but it was of sta-tistically significant differences in NGT group and IGR group. Central obese indexes( WC、WHR、WHtR) showed a positive correlation to PWV in the studied groups(P0. 05). After adjusting for age, gender and cardiovascular risk factors, the multiple regression analysis found that for every 0. 1 point increase in WHR and WHtR, the PWV increased 40. 6 cm/s and 55. 3cm/s respectively. Conclusion There is a positive correlation between central obese indexes (WC、WHR、WHtR) and arterial stiffness, and the central obese indexes correlated with arterial stiffness better than BMI.

13.
Article in Chinese | WPRIM | ID: wpr-306329

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the occupational safety climate in different types of enterprises and its relationship with occupational accidental injury.</p><p><b>METHODS</b>A cross-sectional survey based on self-report questionnaires was performed among 3311 front-line workers from 54 medium and small-sized manufacturing enterprises of different types in Zhongshan, China to investigate the socio-demographic characteristics, safety climate experience in workplace, and incidence of occupational accidental injury in the past year.</p><p><b>RESULTS</b>Analysis of the data revealed that employees in different types of companies perceived different levels of safety climate, according to the scores on four subscales; the European and American enterprises had significantly better safety climate than the Hong Kong and Chinese private enterprises (P < 0.01). The self-reported rates of occupational injury were 3.38%, 4.76%, and 6.72%, respectively, for European and American, Hong Kong, and Chinese private enterprises (χ(2) = 6.78, P < 0.05). After control of such factors as age, sex, income, education level, and marriage, the logistic regression analysis showed that the risk of occupational accidental injury in the European and American enterprises was significantly lower than that in the Chinese private enterprises (OR = 0.57, 95%CI = 0.35-0.91).</p><p><b>CONCLUSION</b>The type of enterprise influences the occupational safety climate and incidence of occupational injury among workers.</p>


Subject(s)
Accidents, Occupational , Adolescent , Adult , China , Cross-Sectional Studies , Europe , Female , Humans , Male , Middle Aged , Occupational Health , Occupational Injuries , Epidemiology , Surveys and Questionnaires , United States , Workplace , Young Adult
14.
Article in Chinese | WPRIM | ID: wpr-306282

ABSTRACT

<p><b>OBJECTIVE</b>To determine the prevalence of occupational musculoskeletal disorders (OMSD) and its risk factors among workers in three manufacturing industries in Zhongshan, China by cross-sectional epidemiological investigation.</p><p><b>METHODS</b>A total of 2 035 workers from the industries of metals (1001 persons), electrical appliances (455 persons), and furniture (579 persons), including 1 402 males and 633 females, were selected; the mean age was 32.9 ± 8.2 years, and the mean length of service was 6.4 ± 5.6 years. A revised Northern Europe Standardized Questionnaire was used for cross-sectional epidemiological investigation of OMSD.</p><p><b>RESULTS</b>The results showed that OMSD in these workers was primarily located in the neck, waist, and shoulder, with annual prevalence rates of 23.1%, 20.1%, and 15.8%, respectively. The overall prevalence of OMSD was 43.1% in metal industry, 44.0% in electrical appliance industry, and 26.6% in furniture industry. OMSD prevalence showed significant differences between different industries (χ(2) = 54.2, P < 0.01). The prevalence of OMSD in the shoulder and back increased with working years (P < 0.05). Logistic regression analysis showed that the risk factors for OMSD were working age >10 years, safety behavior such as "bending down when lifting heavy things from the ground", and different types of industries.</p><p><b>CONCLUSION</b>OMSD is mainly manifested by neck pain, waist pain, and shoulder pain among front-line manufacturing workers in Zhongshan, and working age, poor labor posture, and different types of industries were risk factors for waist pain.</p>


Subject(s)
Adult , China , Epidemiology , Cross-Sectional Studies , Female , Humans , Male , Musculoskeletal Diseases , Epidemiology , Occupational Diseases , Epidemiology , Prevalence , Risk Factors
15.
Chinese Journal of Biotechnology ; (12): 1278-1289, 2013.
Article in Chinese | WPRIM | ID: wpr-242482

ABSTRACT

To enhance biohydrogen production of Klebsiella sp. HQ-3, the global transcriptional factor (Fnr), formate dehydrogenase H (FDH1) and the pncB gene encoding the nicotinic acid phosphoribosyltransferase (NAPRTase) were for the first time over-expressed in Klebsiella sp. HQ-3. The fnr, fdhF, pncB genes were cloned from the genomic DNA of Klebsiella sp. HQ-3 by 3 pairs of universal primers, and introduced into the corresponding sites of the modified pET28a-Pkan, resulting in the plasmids pET28a-Pkan-fnr, pET28a-Pkan-fdhF and pET28a-Pkan-pncB. The 4 plasmids were then electroported into wild Klebsiella sp. HQ-3 to create HQ-3-fnr, HQ-3-fdhF, HQ-3-pncB and HQ-3-C, respectively. Hydrogen production was measured using a gas chromatograph and the metabolites were analyzed with a high-performance liquid chromatograph (HPLC). The results indicate that over-expression of fnr, fdhF and pncB significantly enhanced hydrogen production in the three recombinant strains. Hydrogen production per mol glucose for HQ-3 fnr, HQ-3 pncB, HQ-3 fdhF was 1.113, 1.106 and 1.063 mol of hydrogen/mol glucose, which was respectively increased by 12.26%, 11.62% and 7.28% compared with that of the control strain HQ-3-C (0.991 mol of hydrogen/mol glucose). Moreover, the analysis of HPLC showed that the concentrations of formate and lactate were markedly decreased, but succinate remained unchanged in culture media compared with those of the control strain HQ-3-C.


Subject(s)
Fermentation , Formate Dehydrogenases , Genetics , Hydrogen , Metabolism , Iron-Sulfur Proteins , Genetics , Klebsiella , Genetics , Metabolism , Metabolic Engineering , Methods , Metabolic Networks and Pathways , Pentosyltransferases , Genetics
16.
Article in Chinese | WPRIM | ID: wpr-746733

ABSTRACT

Neural plasticity is one of the most important research area of developmental neurobiology. NMDA(N-Methyl-D-aspartate) receptor is one of the glutamate receptors in nervous system, which palys an important role in many biological and pathological changes, such as development of neural network, neural plasticity, learning and memory, degeneration of the neurons and so on. The studies on NMDA receptor with auditory plasticity and learning and memory were reviewed in order to make early intervention for hearing impaired children and provide a theoretical basis.


Subject(s)
Auditory Perception , Humans , Learning , Memory , Nervous System , Neuronal Plasticity , Receptors, N-Methyl-D-Aspartate
17.
Chinese Journal of Nephrology ; (12): 753-757, 2010.
Article in Chinese | WPRIM | ID: wpr-383171

ABSTRACT

Objectives To investigate the relationship between albuminuria and all-cause mortality and cardiovascular mortality in middle-to-old-aged Chinese population. Methods A total of 2500 residents aged more than 40 years old were selected using random cluster sampling in Shougang community, Beijing, and 2315 of them took part in the survey finally. Morning urinary samples were collected. Urinary albumin and creatinine were measured. Albumin to creatinine ratio (ACR) was calculated and used as an index of albuminuria. The subjects were grouped according to ACR: normoalbuminuria (NO, ACR< 30 mg/g), microalbuminuria (MI, ACR 30-299 mg/g), and macroalbuminuria (MA, ACR ≥ 300 mg/g). Albuminuria (AL) group consisted of MI group and MA group. Cardiovascular risk factors were also investigated. Then all-cause mortality and cardiovascular mortality were collected after 4 years. The Cox model was used to analyze the relationship between albuminuria and all-cause mortality after adjusting for confounders. Results The prevalence of microalbuminuria and macroalbuminuria was 7.6% and 1.4% respectively. After 4 years follow-up,the cardiovascular mortality was 2.7/1000 person-years in NO group, 19.9/1000 person-years in MI group, and 11.5/1000 person-years in MA group and the all-cause mortality was 6.6/1000,25.9/1000 and 57.5/1000 person-years respectively. After adjusting for age, gender, smoking, body mass index, serum lipids, hypertension, diabetes mellitus, cardiovascular disease at baseline and serum creatinine, the hazard ratio (HR) of cardiovascular mortality in AL group was 5.26 [95% confidence intervals (CI) 2.26-12.24] compared with NO group; the HR of all-cause mortality was 3.34 (95% CI 1.82-6.15). Among patients without cardiovascular disease at baseline, the corresponding HRs were 6.92 (95%CI 1.80-26.58) and 2.85 (95%CI 1.22-6.65) respectively.Conclusion In the population studied, albuminuria is an independent risk factor for all-cause mortality and cardiovascular mortality.

18.
Article in Chinese | WPRIM | ID: wpr-396606

ABSTRACT

Objective To investigate the outcome and related risk factors of integrated intensive intervention in participants with impaired glucose regulation (IGR) after two years by the criteria of American Diabetes Association 2003. Methods The subjects who remained to be IGR at the end of first year following 75 g oral glucose tolerance test were randomly assigned to either a routine care control group or to an intensive integrated intervention group. The control group received general dietary and exercise advice at baseline and was followed up. In addition to dietary control and exercise advice, mefformin or acarbose were administrated in the intervention group. The latter group was also advised to take antihypertensive agents, lipid-regulating agents if necessary, as well as aspirin. Results The proportion of patients who fulfilled the assigned goals of blood glucose, blood pressure, body mass index or triglycerides was significantly higher in the intensive group than those in the control group. None in the intensive group developed overt diabetes mellitus, while 8 (9.3%) in the control group did. The proportion of patients who reverted to normal glucose tolerance (NGT) was slightly higher in the intensive group than in the control group (29.5% vs 22.1%, P>0.05). Logistic analysis showed that increase of waist circumference and systolic blood pressure was positively while the improvement of islet β-cell function was negatively correlated with the development of diabetes mellitus. Conclusions The intensive integrated intervention could significantly decrease the conversion rate of IGR to diabetes mellitus, and increase the chance of reversion to NGT. The increase of waist circumference or systolic blood pressure, the deterioration of islet β-ccll function were the influencing factors of the conversion of IGR to diabetes mellitus.

19.
Article in Chinese | WPRIM | ID: wpr-394003

ABSTRACT

Objective To evaluate the application of domestic probe fluorescence in situ hybridization (FISH) in prenatal diagnosis on uncultured amniocytes aneuploid. Methods One thousand three hundred and sixty-nine uncultured amniocytes (16-24 gestational weeks) from 37 hospitals in China were selected for prenatal diagnosis. 5 chromosomes (21, 13, 18, X and Y) were detected with muhicolor FISH. In the mean time, cytogenetic karyotype analysis was performed as control. Results Of all the samples, 1361 samples were successfully tested by FISH, the rate of successful detection was 99.42% (1361/1369). Thirty-five samples were shown with abnormal karyotypes by domestic FISH probe, the abnormal rate is 2. 57% (35/1361 ), including trisomy 21 (22 samples), trisomy 13 (4 samples), trisomy 18(6 samples), X0 (1 sample) and XXY (2 samples). Results of both FISH and cytogenetic karyotype analysis exhibited extreme concordance. Conclusion Domestic FISH probe used in prenatal diagnosis on uncultured aminiocytes showed the following advantages, such as highly efficient, low cost, small amounts of samples needed and reliable results.

20.
Chinese Journal of Dermatology ; (12): 525-528, 2009.
Article in Chinese | WPRIM | ID: wpr-393607

ABSTRACT

Objective To report a case of vaginal colonization due to Trichosporon inkin. Methods A 34-year-old female presented with increased vaginal discharge accompanied by abnormal odor for 2 months. Clinical laboratory examination was carried out. Cultures of vaginal discharge yielded yeast-like colony. Subsequently, the isolate underwent the following mycological examinations: purification, slide micro-culture, temperature test, urea enzyme test, biochemistry identification, antifungal susceptibility test, and gene sequencing. Results Gynecological examination revealed white homogeneous secretions attached to mucous membrane of the vagina. Nugent scores of vaginal discharge amounted to 5-6. Two rounds of culture of vaginal discharge resulted in stramineous, reductus and yeast-like colony. The isolate could grow in 42 ℃. Appressorium on the top of hypha and typical sarcinae formed in slide microculture of corn agar, and yeast malt agar was the optimal growth medium for it. Urea enzyme test was positive. API 20C AUX biochemical test and gene sequencing revealed that the isolate was consistent with Trichosporon inkin. The isolate was sensitive to amphotericin B and azoles such as clotrimazole and fluconazole, but resistant to flucytosine and caspofungin. Conclusions It is the first report of vaginal colonization due to T. Inkin in China. The accu-rate identification of T. Inkin relies on synthetic analysis of phenotype characteristics, biochemistry test and molecular sequencing.

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