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Objective To test the inhibitory effect of Corynebacterium cell wall extract on bladder cancer cells. Methods The bladder RNA was extracted from bladder cancer rats, and concentration and purity of RNA was detected. The extracted RNA was reversely transcribed into cDNA, then the primers were designed and the Grim19 gene, β-actin gene, Stat3 gene was amplified. Finaly the PCR product was subjected to agarose gel electrophoresis. Results OD260 was 0.07, and OD260/OD280 was 2.03 in the group of Corynebacterium parvm extract(3-5# ); OD260 was 0.12, and OD260/OD280 was 2.07 in the group of MNU( 6-5#);OD260 was 0.08, and OD260/OD280 was 2.07 in the group of physiological saline(7-5#) . The results of agarose gelelectrophoresis showed that Grim19 gene and Stat3 gene was expressed highly in the bladder of rats from the group of CP cell wall extract (3-5# ). Grim19 gene was expressed lowly, while Stat3 gene was expressed highly in the bladder of rats from the group of MNU (6-5#). Grim19 gene and Stat3 gene was expressed normally in the bladder of rats from physiological saline(7-5). Conclusions The expression situation of antitumor gene Grim19 and tumor gene Stat3 in the bladder of rats was inhibited by the cell wall extract of Corynebacterium parvm, which indicates that the cell wall extract of Corynebacterium parvm has inhibitory effect on bladder cancer cells.
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To facilitate noninvasive diagnosis of anemia, high-performance and portable near infrared (NIR) spectrometer for human blood constituents was designed and fabricated based on linear variable filter (LVF).Meanwhile, the performance of support vector regression (SVR) model for quantitative analysis of human hemoglobin (Hb) was investigated.Spectral data were collected noninvasively from 100 volunteers by self-designed LVF-NIR spectrometer, then divided into calibration set, validation set 1 and 2.To establish a robust SVR model, grid search method was applied to optimize the penalty parameter and kernel function parameter c=5.28, g=0.33.Then, Hb levels in the validation 1 and 2 sets were quantitatively analyzed.The results showed that the root mean square error of prediction (RMSEP) were 10.20 g/L and 10.85 g/L, respectively, and the relative RMSEP (R-RMSEP) were 6.85% and 7.48%, respectively.The results indicated that the SVR model had high prediction accuracy to Hb level and adaptability to different samples, and could satisfy the requirements of clinical measurement.Based on the SVR algorithm, the self-designed LVF-NIR spectrometer has a wide application prospect in the field of non-invasive anemia diagnosis.
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To analyze the curative effects of the transvaginal myomectomy [TVM] and the traditional transabdominal myomectomy [TAM]. The group with traditional TAM received routine preoperative nursing guidance and postoperative care. While the group with TVM received preoperative strengthened psychological care, vaginal preparation and postoperative strengthened vagina nursing, paying attention to the prevention and treatment of complications, and maintaining effective pelvic draining and other nursing measures. After that, the statistical processing and analysis were used to compared the intraoperative blood loss, operating time, postoperative infusion time, hospitalization time, postoperative eating, postoperative ambulation, the usage rate of postoperative analgesic, postoperative morbidity and postoperative complications between the two groups Comparison between TVM group and TAM group showed that the difference of the operating time, postoperative infusion time, hospitalization time, postoperative eating, postoperative ambulation, the usage rate of postoperative analgesic, postoperative morbidity and postoperative complications between the two groups were statistically significant [P<0.05]. While the difference of intraoperative blood loss between the two groups were not statistically significant [P>0.05]. The operating time in TVM group was shorter than TAM group; the postoperative eating, postoperative ambulation were earlier than the TAM group; the duration of postoperative transfusion and hospitalization in TVM were shorter than the TVM group; and the usage rate of postoperative analgesic and postoperative morbidity and complications in TVM group were less than the TAM group. TVM have the advantages of minimal invasion, quick recovery, less pain, shorter hospitalization duration and notable curative effect etc. Strengthening nursing care is the key to the success of treatment
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Aim To investigate the effect of Zhizi Baipi soup and its disassembled prescription containing Zhizi on protecting concanavalin (ConA)-induced immuno-logical liver injury in mice and explore the possible protective mechanism.Methods The model of mouse immunological liver injury induced by (ConA,20 mg ·kg -1 )was used to observe the effects of Zhizi Baipi soup and its disassembled prescription containing Zhizi by gavage administration.Results Zhizi Baipi Soup and its disassembled prescription containing Zhizi were able to reduce the level of serum ALT,AST and MDA content,improve liver tissue SOD activity and reduce the expression of NF-κB-p65 and NF-κB-p-p65,in which all parties of Zhizi Baipi Soup Group had best effect on immunological liver injury in mice.Conclu-sion Zhizi Baipi Soup and its disassembled prescrip-tion containing Zhizi have remarkably protective effect on ConA-induced immunological liver injury in mice, and the potential mechanism may be related to the reg-ulation of the immune response pathway.
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Objective To investigate the effects of dynamic ventilatory factors on inflammatory response and function of extrapulmonary organs in acute respiratory distress syndrome (ARDS) dogs. Method Thirty-six healthy dogs were randomly divided into 6 groups: normal control group (N group), ARDS group ( M group) and ventilation groups (A~D groups)hased on a series of random number. The ARDS dog model was replicated by iutrawacheal instillation of hydrochloric acid and mechanical ventilation was carried out according to the following protocols. Group A:low VT, low inspiratory flow and high respiratory rate. Group B: large VT, high inspiratory flow and high respiratory rate. Group C: large VT, high inspiratory flow and low respiratory rate. Group D: large VT, low inspiratory flow and low respiratory rate. After 4 hours of mechanical ventilation, animals were sacrificed.Tumor necrosis factor (TNF)-α and intefleukin (IL)-8 levels in blood serum were measured by radioimmunoassay.Histopathological changes of liver and kidney were observed under light microscope. Results The level of IL-8 and TNF-u of group B and C were much higher than those of group D, A and M ( P<0.05), but there was no significant difference among group D, A and M ( P>0.05). The level of IL-8 and TNF-α of group N were much lower than other groups ( P<0.05). The change of histopathology was the most significant in group B and C while the change of histopathology on group C was better than group B. The change of histopathology on group A and D was better than group B and C. The change of group A was similar to group M. All the data were analyzed by statistical F test. There was statistical difference with P<0.05. Comclusions Large tidal volumes with high inspiratory flow and high respiratory rate may increase mediators of inflammation in blood sennn, and aggravate the irflamnnmtory response of extrapulmonary organs. Beducfion of inspiratory flow and respiratory rate ventilation may prevent the occurrence d multiple organ dysfunction syndrome.
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OBJECTIVE To analyze the bacterial distribution and drug-sensitivity in sputum bacterial culture of the hospitalized patients with respiratory tract infection in the department of respiratory medicine to help reasonably choose the antibiotics and reduce the nosocomial infection rate. METHODS The bacterial infection of all the hospitalized patients with lower respiratory tract infection in the department of respiratory medicine from 2004 to 2005 was analyzed retrospectively and compared with all the patients of the hospital. RESULTS The nosocomial infection rate of the department of respiratory medicine in 2005 was 10.18%,which was higher than the rate of nosocomial infection of the whole hospital(3.09%).The Gram-negative bacteria were the main ones infected through respiratory tract.What should be paid attention to was the comparably higher fungal infection rate in 2005.The main Gram-negative bacteria were dominated by Pseudomonas aeruginosa.The most sensitive drug was piperacillin+ tazobactam in 2004 and aminoglycoside antibiotics in 2005.The Gram-positive bacteria were most sensitive to vancomycin. CONCLUSIONS The nosocomial infection is dominated by lower respiratory tract infection in the department of respiratory medicine.The nosocomial infection rate,dominated by Gram-negative bacteria is high.In order to decrease nosocomial infection rate,cross infection should be avoided,sensitive antibiotics be chosen,body resistance be enhanced and hospital stay be reduced.