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BACKGROUND:Photothermal therapy is a novel tumor treatment strategy that uses photothermal agents to transform light energy into heat energy to accomplish non-invasive tumor ablation.The rise of photothermal therapy and nanotechnology has provided a new perspective on breast cancer treatment.OBJECTIVE:To prepare a new type of near-infrared biomimetic nanoprobe that has been modified by breast cancer cell membrane,to investigate the effect of near-infrared fluorescence/ultrasound imaging in vitro,and to observe its targeting ability and photothermal therapy effect on homologous tumor cells in vitro.METHODS:Organic small molecule ITIC-4CI with A-D-A structure was used as photothermal agents;polylactic acid/glycolic acid copolymer as nanocarrier;4T1 cell membrane of mouse breast cancer cells as a surface modifier of nanoparticles;perfluorohexane(PFH)was loaded.A novel near-infrared biomimetic nanoprobe(4T1m/ITIC-4CI/PFH)was prepared by the double emulsion evaporation method and sonication method.The basic characterization of the nanoprobe and the homologous targeting ability were detected.The photothermal properties and photothermal stability of the probe were investigated,and the near-infrared fluorescence/ultrasound imaging effect of the probe under laser irradiation was observed.The CCK-8 assay and calcein/propidium iodide staining were used to assess the efficacy of photothermal therapy.RESULTS AND CONCLUSION:(1)The prepared 4T1m/ITIC-4CI/PFH nanoprobes had uniform size,high stability,and an average particle size of(92.7±2.3)nm.The probe's protein composition was identical to that of the 4T1 cell membrane.The nanoprobe's ability to target homologous 4T1 cells was validated by an in vitro cell uptake assay.(2)The nanoprobe had a red-shift absorption spectrum and tail emission extending to the near-infrared-Ⅱ,which emitted a bright near-infrared-Ⅱ fluorescence signal under laser irradiation.(3)After laser irradiation,the nanoprobe 4T1m/ITIC-4CI/PFH could be turned into microbubbles and enhanced ultrasound imaging.The results of CCK-8 assay and calcein/propidium iodide staining showed that the nanoprobe 4T1m/ITIC-4CI/PFH had an obvious photothermal killing effect on 4T1 cells.(4)The results show that the nanoprobe 4T1m/ITIC-4CI/PFH has the ability to target homologous tumors and enhance near-infrared-Ⅱ fluorescence imaging/ultrasound imaging and photothermal therapy effects.
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By reviewing ancient materia medica, medical books, prescription books and modern literature, this paper conducted a systematic research on name, origin, medicinal parts, producing area, quality, harvesting and processing methods, functions and toxicity of Polygoni Multiflori Radix(PMR) and Polygoni Multiflori Caulis(PMC) in famous classical formulas. It was found that the name of Heshouwu was first found in the Biography of Heshouwu and originated from its discoverer, and then still in use today. Since the Song dynasty, Heshouwu has been included in the materia medica with Fallopia multiflora as the mainstream origin. Since the Ming dynasty, in addition to F. multiflora, Cynanchum bungei, C. wilfordii, C. auriculatum have been used as the origin of Heshouwu. Heshouwu is widely distributed, the production areas recorded in ancient times are mainly Luchuan, Guangxi and Ganzhou, Jiangxi, and in modern times, Deqing, Guangdong and other places are respected as the geo-authentic habitats. Its origin processing is mostly dug out, washed, sliced and dried in the sun. Modern literature concludes that the quality of PMR is better in terms of weight, solid quality and powder, while PMC is better in terms of uniformity and purplish-red skin. In the Qing dynasty and before that, it was recorded in the materia medica that PMR was harvested mainly in late spring, mid-summer and early autumn, in modern times, it was harvested mainly in spring and autumn, while PMC was harvested in autumn. In Song dynasty, the processing method of PMR was mainly soaked with rice-washed water, the Ming dynasty for black bean steaming method, the Qing dynasty for steaming followed by black bean juice maceration, while in modern times, it is mostly sliced and steamed with black bean juice and yellow rice wine until the juice is absorbed. PMC is prepared by removing impurities, washing or moistening, and cutting into sections to dry. During the five dynasties, PMR was used to treat infertility as well as gastrointestinal diseases and blood in the stool caused by wind chill, and during the Song dynasty, it was mostly used raw products for the treatment of scrofula and carbuncles, and in the Yuan and Ming dynasties, raw and processed products were used, and Polygoni Multiflori Radix Praeparata was used to treat hemorrhoids, waist and knee soreness. PMC is mainly used for treating insomnia, fatigue and sweating, wind sores and scabies, etc. In the Song dynasty, the ancients considered that PMR was non-toxic, but its toxicity was gradually discovered after the Ming dynasty, and the toxicity mechanism has not been clearly analyzed yet. Based on the results of the textual research, it is recommended that F. multiflora be used as the base for development of famous classical formulas containing PMR, and from the safety perspective, it is recommended to use raw products with caution, oral administration must be used processed products. PMC is recommended to use raw products.
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By reviewing ancient materia medica, medical books and modern literature, the name, origin, quality evaluation, producing area and processing methods of Huoxiang herbs were systematically investigated and researched, so as to provide reference and basis for the development and utilization of famous classical formulas containing Huoxiang herbs. Through the herbal textual research, it can be seen that most of materia medica in past dynasties have taken Huoxiang as the nominal rectification, and the mainstream base used is Pogostemon cablin. In order to distinguish another plant of the same family, Agastache rugosa, which has been widely used in Chinese folk since the Ming dynasty, and respect geo-authentic region, Pogostemonis Herba is also named Guanghuoxiang. Pogostemonis Herba is native to Vietnam and other Southeast Asian countries, and was introduced to China as a spice through Guangdong and other places in the early days, and has been successfully cultivated in the south of China since the Song dynasty. The medicinal parts are mostly dried aboveground parts, and the leaves and stems are also separated for medicine sometimes. The geo-authentic region of Pogostemonis Herba is Guangdong in the past dynasties, and it is currently cultivated in Guangzhou, Zhaoqing, Zhanjiang of Guangdong province and Hainan province, with the most famous one cultivated in Shipai. Pogostemonis Herba is mainly planted by cutting propagation. It usually sprouts in February and is harvested in June. The main processed method in region is stuffy dry, which is placed in the sun and repeatedly suffocated until it has an aromatic smell and the color turns yellow. The processing method is mostly to use the raw product as medicine after being selected. Based on the research results, it is suggested that the leaves of P. cablin are used in Yangweitang, for Huopo Xialingtang, it is recommended to choose the raw product of A. rugosa that is removed the roots and old stems.
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OBJECTIVE To study main way and target of Euphorbia kansui after stir-frying with vinegar. METHODS Twenty-four SPF grade SD rats were randomly divided into blank group, E. kansui group (850 mg/kg) and vinegar stir-fried E. kansui group (850 mg/kg), with 8 rats in each group. Blank group was given 0.5% sodium carboxymethyl cellulose solution intragastrically, and E. kansui group and vinegar stir-fried E. kansui group were given relevant test sample for consecutive 20 d. The rats’ urine of 12 hours was collected on the 20th day. The urine samples of rats in each group were determined by UPLC-Q- Exactive-MS. The data was pre-processed by Compound Discoverer 3.0 software, and the metabolite structure was identified by BioCyc, HMDB and other databases. Whether different groups presented their own clustering phenomenon was observed by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), etc. Based on the pathway analysis of MetaboAnalyst, the potential targets of detoxification mechanism of E. kansui after stir-frying with vinegar were predicted. RESULTS Twenty significantly differential endogenous metabolites were identified, of which 10 target metabolites, such as N-acetyl-L-aspartate and 3-phosphonooxypyruvic acid, were targets of detoxification mechanism of E. kansui after stir- frying with vinegar. The main metabolic pathways included arginine biosynthesis, alanine, aspartic acid and glutamic acid metabolism, cysteine and methionine metabolism, and arginine and D-ornithine metabolism. The biological significance of all related metabolites in the pathways was analyzed and speculated; after stir-frying with vinegar, E. kansui may alleviate neurotoxicity by reducing the level of N-acetyl-L-aspartic acid; E. kansui had a protective effect on cardio-cerebrovascular system by increasing the level of L-high arginine. CONCLUSIONS After stir-frying with vinegar, E. kansui can significantly improve the adverse factors in terms of nervous system, cardio-cerebrovascular system, immune system and energy metabolism. The most concentrated metabolic pathway related to its detoxification mechanism is arginine biosynthesis.
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OBJECTIVE To screen the active ingredient with estrogenic effect from total flavonoids of Cuscutae Semen. METHODS The estrogenic effect of total flavonoids from 10 batches of Cuscutae Semen was evaluated with mouse uterus coefficient and endometrial thickness as evaluation indexes, establish its fingerprint and calibrate the common peak. Common peak and spectrum-effect relationship of the above two indicators were analyzed by bivariate relationship analysis and grey correlation analysis to screen active components with estrogenic effect. UPLC-Q-TOF-MS technology was used to characterize the active components. RESULTS The estrogenic effect of total flavonoids from 10 batches of Cuscutae Semen was good. Twenty-eight and thirty-three common peaks of total flavonoids in Cuscutae Semen were obtained in the positive and negative ion modes respectively. The constituents represented by peaks 7,10,12-16,26 in positive ion mode and peaks 2,5,8,9,12,16,19,22-26 in negative ion mode were highly correlated with the estrogenic effect of total flavonoids from Cuscutae Semen. Further identification showed that the active substances with estrogenic effect from the total flavonoids of Cuscutae Semen were 5,7,3′, 4′-tetramethoxyflavone, 6- O-(trans) p-coumarin-furanfructose-(2→1)-glucopyranoside, rutin, kaempferol-3,7-diglucoside, apigenin-7-O-glucoside, hyperoside, baicalin, quercitin, quercetin, apigenin, kaempferol, isorhamnetin, rhododendron, isoquercetin, kaempferol-3-furan arabinoside, 2,6-octadecanediacetylic acid. CONCLUSIONS A total of 16 chemical components with estrogenic effect are screened from total flavonoids of Cuscutae Semen in the study, which can provide reference for the development of phytoestrogens.
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ABSTRACT OBJECTIVE:To study the metabolic transformation of total glycosides of Cistanche deserticola in artificial gastric and intestinal juice,and to speculate its metabolic transformation pathway in vivo. METHODS:UPLC/Q-TOF-MS was adopted. The determination was performed on ACQUITY UPLC BEH column with mobile phase consisted of 0.2% formic acid water-acetonitrile(gradient elution)at the flow rate of 0.2 mL/min. The detection wavelength was set at 330 nm,and column temperature was 25 ℃. The ion source was electrospray ion source,and mass to charge ratio(m/z)was 50→1 000. In the positive and negative ion mode,the metabolic components of the total glycosides of C. deserticola in artificial gastric and intestinal juice were identified analysis,and combined with the literature,the metabolic pathway of total glycosides of C. deserticola in artificial gastric and intestinal juice was speculated. RESULTS:After the total glycosides of C. deserticola were metabolized by artificial gastric juice,and a total of 69 components were estimated,including 14 prototype components (such as Mustard aldehyde glucoside,daucosstorol) and 55 metabolic components (such as Methyl-O-Kankanoside J,Methyl-O-Kankanoside E),it is speculated that its metabolic pathways were methylation,demethylation,hydroxylation,methoxylation,acetylation,sulfation,and glucuronidation. After the total glycosides of C. deserticola were metabolized by artificial intestinal juice,a total of 90 components were estimated,including 4 prototype components(such as Kankanoside M,Kankanoside L)and 86 metabolic components(such as Methyl-O-Kankanoside, Methyl-O-Kankanoside E). It was speculated that its metabolic pathways were methylation, demethylation,hydroxylation,dehydroxylation,methoxylation,acetylation,sulfation and glucuronidation. CONCLUSIONS:This study preliminarily speculates that the total glycosides of C. deserticola may be metabolized by methylation,demethylation, hydroxylation and other metabolic pathway in artificial gastrointestinal juice,which may provide reference for the in vivo metabolic transformation of total glycosides of C. deserticola.
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OBJECTIVE:To study the prepar ation technology of gastric floating tablets of Schisandra chinensis total lignans (SCTL),and evaluate the quality of prepared tablets. METHODS :Based on single factor test ,the orthogonal experiment was conducted to optimize the formulation of SCTL gastric floating tablets with the contents of hydroxypropylmethylcellulose (HPMC) K15M,NaHCO3 and microcrystalline cellulose as the factors ,using starting time ,holding time and cumulative release rate of gastric floating tablets as evaluation indexes. The properties ,weight difference ,floatability and accumulative release rate of the prepared SCTL gastric floating tablets were determined. The gastric floating tablets were qualitatively identified by TLC ,and the contents of schisandrin A and total lignans were determined by HPLC and UV spectrophotometry. RESULTS :The optimal formulation of SCTL gastric floating tablets was made up of 23% SCTL extract ,20% HPMC K 15M,40% microcrystalline cellulose,15% sodium bicarbonate ,1% octadecyl alcohol and 1% polyvinylpyrrolidone. The results of detection of this preparation were in line with the related provisions of “0101 tablet”stated in 2015 edition of Chinese Pharmacopoeia (part Ⅳ). TLC indicated that the chromatogram of the test sample showed the main spots of same color as the corresponding positions of the chromatogram of schizandrol A control ,Schisandra chinensis reference substance and raw material ,while the negative control has no interference. Content determination results shows that the average content of schizandrol A and total lignans in SCTL gastric floating tablets is 3.187,19.617 mg. It was preliminarily formulated that the content limitation of schizandrol A in one tablet should not be less than 2.50 mg,and the content of total lignans (calculated by schizandrol A )should not be less than 15.50 mg. CONCLUSIONS:The preparation technology of SCTL gastric floating tablets is stable ,feasible and controllable in quality.
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Carotid artery vulnerable plaque is prone to rupture and thrombosis,which is closely related to the occurrence of stroke.The detection of carotid plaque vulnerability is particularly important for early risk assessment of cerebrovascular disease.Carotid ultrasound is noninvasive,less expensive and with high reproducibility,therefore has become the important method for detecting the vulnerability of carotid artery plaque.The progresses of ultrasound technique for detection of carotid vulnerable plaque in recent years were reviewed in this article.
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Objective To evaluate the role of tumor necrosis factor alpha-inducible protein 8 like-2 ( TIPE2) in macrophage pyroptosis in mice. Methods Mouse macrophages J774A. 1 were seeded in 6-cm culture dishes (5 ml∕dish) at the density of 2×105 cells∕ml and divided into 4 groups (n=18 each) using a random number table method: blank vector control group ( C group) , blank vector plus lipopolysaccharide ( LPS)∕ATP group ( C+LPS∕ATP group) , TIPE2 overexpression group ( T group) and TIPE2 overexpres-sion plus LPS∕ATP group ( T+LPS∕ATP group) . Cells were infected with lentivirus without TIPE2 in C and C+LPS∕ATP groups. TIPE2 overexpression stable cell line was constructed in T group and T+LPS∕ATP group. LPS 1. 0 μg∕ml was added and cells were incubated for 5 h, and then ATP 5. 0 mmol∕L was added and cells were incubated for 1 h in C+LPS∕ATP group and T+LPS∕ATP group. Cells were collected for de-tection of the expression of TIPE2, NOD-like receptor protein 3 ( NLRP3) , interleukin-1beta ( IL-1β) and interleukin-8 ( IL-18) by Western blot. Flow cytometry was used to detect the pyroptotic cells, and the per-centage of pyroptotic cells was calculated. The concentrations of tumor necrosis factor-alpha ( TNF-α) , IL-6, IL-1β and IL-18 in cell culture media were determined by enzyme-linked immunosorbent assay. Results Compared with group C, the expression of TIPE2 was significantly down-regulated, the expression of NL-RP3, IL-1β and IL-18 was up-regulated, and the percentage of pyroptotic cells and concentrations of TNF-α, IL-6, IL-1β and IL-18 in cell culture media were increased in group C+LPS∕ATP (P<0. 05). Com-pared with group T, the expression of TIPE2 was significantly down-regulated, the expression of NLRP3, IL-1βand IL-18 was up-regulated, and the percentage of pyroptotic cells and concentrations of TNF-α, IL-6, IL-1β and IL-18 in cell culture media were increased in group T+LPS∕ATP ( P<0. 05) . Compared with group C+LPS∕ATP, the expression of TIPE2 was significantly up-regulated, the expression of NLRP3, IL-1β and IL-18 was down-regulated, and the percentage of pyroptotic cells and concentrations of TNF-α, IL-6, IL-1β and IL-18 in cell culture media were decreased in group T+LPS∕ATP ( P<0. 05) . Conclusion TIPE2 can inhibit macrophage pyroptosis, and the mechanism may be related to inhibiting activation of NL-RP3 inflammasome in mice.
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Objective To investigate distribution of carotid plaques,degree of stenosis and risk factors of unstable plaques in patients with acute ischemic stroke.Methods Totally 909 patients with acute ischemic stroke (AIS group) confirmed with head MRI were enrolled,while 885 patients without stroke in the same period hospitalization were selected as controls (control group).The distribution of carotid plaques and the degree of stenosis were compared between the 2 groups.Patients in AIS group were divided into stenosis rate ≥50% subgroup and <50% subgroup according to the degree of carotid artery stenosis,and the detectable rate of stable plaques and unstable plaques were compared between the 2 groups.Clinical data of patients with unstable plaques and non-unstable plaques in AIS group were compared.Logistic regression models were established with parameters with significantly differences among those features.Results The detectable rates of multiple plaques and unstable plaques of carotid artery and the degree of stenosis in AIS group were statistically higher than those in control group (all P<0.001).The detectable rate of unstable plaque in carotid artery stenosis rate ≥50% group was statistically higher than that in stenosis rate <50% group (P<0.001).Age,male,diabetes,dyslipidemia,smoking and drinking were significantly risk factors (all P<0.05).Age,diabetes,dyslipidemia and smoking were independent risk factors (all P<0.05).Conclusion Carotid unstable plaque and stenosis are closely related to the occurrence of AIS.Age,diabetes,dyslipidemia and smoking are independent risk factors for carotid artery plaque.
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Aim The TRPV1 plasmid was transiently transfected into human embryonic kidney HEK 293T cells to establish the heterologous expression system of TRPV1-channel. Methods The transfection efficiency was confirmed under fluorescence mi-croscope and the TRPV1 protein expression was identified by u-sing Western blot, and the functional characteristics of the chan-nel were studied by using the method of confocal microscopy. Results The transfection rate could reach 40% ~50%; the transfected cells were found to have a clear band at the corre-sponding position that TRPV1 should be, which indicated that TRPV1 channel protein was expressed in the transfected cells. The confocal microscopy imaging result showed that the trans-fected HEK 293T cells were activated by TRPV1 channel ago-nist. Conclusion Transient transfection of HEK 293T cells with TRPV1 channel is successfully constructed and the heterol-ogous TRPV1 channel is verified to have normal calcium-media-ting function.
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Objective To observe the effect of homemade ankle-foot orthopedic band on motor function of lower extremities in children with foot drop post spastic hemiplegic cerebral palsy (CP). Methods 24 spastic hemiplegic CP children were randomly divided into observa-tion group (n=12) and control group (n=12). Both groups accepted roution rehabilitation training. The control group accepted walking train-ing, and the observation group accepted walking training wearing the homemade ankle-foot orthopedic band, for 12 weeks. They were as-sessed with modified Ashworth Scale (MAS) of gastrocnemius, range of motion (ROM) of ankle active dorsiflexion, and D and E domains of Gross Motor Function Measure (GMFM-88) before and after treatment. Results The score of MAS significantly decreased in both groups after treatment (P<0.01), and decreased more in the observation group than in the control group (P<0.05). ROM and scores of D and E do-mains of GMFM-88 significantly increased in both groups after treatment (P<0.01), and increased more in the observation group than in the control group (P<0.05). Conclusion Walking training with the homemade ankle-foot orthopedic band may further decrease the muscle tone of gastrocnemius, increase the active range of motion of ankle and improve the motor function of lower extremities in children with foot drop post spastic hemiplegic CP.
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Objective To observe the effect of nerve mobilization on shoulder pain in hemiplegic patients after stroke. Methods 50 pa-tients with hemiplegic shoulder pain after stroke were randomly divided into control group (n=25) and observation group (n=25). Both groups were given conventional rehabilitation training, and the observation group received nerve mobilization additionally. Number Ana-logue Scale (NAS), Fugl-Meyer Assessment (FMA) and modified Barthel Index (MBI) were used to evaluate the shoulder pain, the the up-per limb motor function and activities of daily living (ADL) before and 4 weeks after treatment. Results The score of NAS decreased in both groups after treatment (P<0.001), and was lower in the observation group than in the control group (P<0.01). The score of FMA and MBI increased in both groups after treatment (P<0.001), and was higher in the observation group than in the control group (P<0.05). Con-clusion Nerve mobilization could further relieve the shoulder pain and improve the motor function of upper limb and ADL in patients with hemiplegia after stroke.
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@#Objective To observe the effect of homemade ankle-foot orthopedic band on motor function of lower extremities in children with foot drop post spastic hemiplegic cerebral palsy (CP). Methods 24 spastic hemiplegic CP children were randomly divided into observation group (n=12) and control group (n=12). Both groups accepted roution rehabilitation training. The control group accepted walking training, and the observation group accepted walking training wearing the homemade ankle-foot orthopedic band, for 12 weeks. They were assessed with modified Ashworth Scale (MAS) of gastrocnemius, range of motion (ROM) of ankle active dorsiflexion, and D and E domains of Gross Motor Function Measure (GMFM-88) before and after treatment. Results The score of MAS significantly decreased in both groups after treatment (P<0.01), and decreased more in the observation group than in the control group (P<0.05). ROM and scores of D and E domains of GMFM-88 significantly increased in both groups after treatment (P<0.01), and increased more in the observation group than in the control group (P<0.05). Conclusion Walking training with the homemade ankle-foot orthopedic band may further decrease the muscle tone of gastrocnemius, increase the active range of motion of ankle and improve the motor function of lower extremities in children with foot drop post spastic hemiplegic CP.
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@#Objective To observe the effect of homemade ankle-foot orthopedic band on motor function of lower extremities in children with foot drop post spastic hemiplegic cerebral palsy (CP). Methods 24 spastic hemiplegic CP children were randomly divided into observation group (n=12) and control group (n=12). Both groups accepted roution rehabilitation training. The control group accepted walking training, and the observation group accepted walking training wearing the homemade ankle-foot orthopedic band, for 12 weeks. They were assessed with modified Ashworth Scale (MAS) of gastrocnemius, range of motion (ROM) of ankle active dorsiflexion, and D and E domains of Gross Motor Function Measure (GMFM-88) before and after treatment. Results The score of MAS significantly decreased in both groups after treatment (P<0.01), and decreased more in the observation group than in the control group (P<0.05). ROM and scores of D and E domains of GMFM-88 significantly increased in both groups after treatment (P<0.01), and increased more in the observation group than in the control group (P<0.05). Conclusion Walking training with the homemade ankle-foot orthopedic band may further decrease the muscle tone of gastrocnemius, increase the active range of motion of ankle and improve the motor function of lower extremities in children with foot drop post spastic hemiplegic CP.
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Objective To explore the genetic association of C1GALT1 gene polymorphisms with susceptibility and prognosis of IgA nephropathy (IgAN) on Uyghur population in Xinjiang Uyghur Autonomous Region.Methods Ninety Uighur patients with IgAN and ninety geographically and age matched healthy controls were recruited.Peripheral blood was collected from recruited individuals for DNA extracting.After amplified by polymerase chain reaction (PCR),genotyping of the four single nucleotide polymorphisms (SNPs) in C1GALT1 gene,which were rs9639031,rs5882115,rs1008898,-527A/G,were detected by direct sequencing analysis.Differences of allele and genotype frequency were analyzed between IgAN and healthy controls.Moreover,the association between these SNPs and the risk and progress in IgAN patients were further analyzed.Results (1) The I allele frequency of rs5882115 in C1GALT1 gene was significantly higher in IgAN than that in healthy controls (x2 =7.788,P =0.015),no difference in allele frequencies of rs9639031,rs1008898,-527A/G between IgAN and healthy controls was found.Under the dominant mode,the DI+ Ⅱ genotype frequencies of rs5882115 was significantly higher in IgAN than that in healthy controls (x2 =8.563,P =0.009),no difference in genotype frequencies of rs9639031,rs1008898,-527A/G between IgAN and healthy controls was found.Under the hidden mode,no difference in genotype frequencies of rs5882115,rs9639031,rs1008898,-527A/G between IgAN and healthy controls was found.Logistic single factor regression analysis showed that the risk to IgAN of whom carry I allele of rs5882115 was 2.469 times than the D allele (OR =2.469),and the risk to IgAN of whom carry DI+ Ⅱ genotype was also higher (OR =2.852).(3) No association was found between these SNPs in C1GALT1 gene and serum creatinine in IgAN.Conclusion Association between rs5882115 in C1GALT1 gene and Uighur IgAN susceptibility suggests that there may be variants in C1GALT1 gene or its linked genetic region,which needs further exploration.
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BACKGROUND: Intravascular stent implantation is one of the main methods for intra-extracranial artery stenosis. The safety and efficacy of stent implantation has been elevated, and symptoms or ischemic advert events with artery stenosis has been reduced. However, risks of thrombosis, cerebralhemorrhage, restenosis, and hyperperfusion still existed. OBJECTIVE: To investigate the influence of atorvastatin on ischemic adverse events following cerebral middle artery stent implantation.METHODS: Twenty-four patients received cerebral middle artery stent implantation were randomly divided into 2 groups. In the treatment group, patients were received atorvastatin 40 mg + plavix 75 mg + aspirin enteric-coated tablet 300 mg, once per day; in the control group, patients were received plavix 75 mg + aspirin enteric-coated tablet 300 rag, once per day. Ischemic adverse events, such as in-stent restenosis, transient ischemic attack, cerebral infarction, or re-interventional therapy were observed by transcranial Doppler, digital subtraction angiography, nuclear magnetic resonance imaging, diffusion weighted imaging, blood lipid level and C-reactive protein level examinations prior to and at months 1, 3, 6 and 12 after stent implantation.RESULTS AND CONCLUSION: Compared with the control group, the levels of blood lipid and C-reactive protein were obviously decreased at months 1, 3, 6 and 12 after treatment (P<0.05, P<0.01 ), with dramatically declined ischemic adverse events (P<0.05, P<0.01 ). Compared with before operation, the levels of blood lipid and C-reactive protein were decreased after operation in the treatment group (P<0.05, P<0.01). No server adverse events occurred in patients treated by atorvastatin, which showed 40 mg was safe for patients. The results revealed that atorvastatin combined with antiplatelet therapy can prevent ischemic adverse events following cerebral middle artery stent implantation.
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Objective To investigate the effect of radix paeoniae rubra (RPR) on expressions of p38MAPK, NF-κB and iNOS in ltpopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its protective mechanism. Methods Forty Wistar rats with ALI were divided randomly into five groups: saline control group (Group A) , LPS group (Group B), RPR for treatment group (Group C), RPR for prevention group (Group D) and SB203580 group (Group E). The effects of RPR on protein content and the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), contents of malondidehyde (MDA) and serum NO in lung tissue were observed. Arterial blood was collected for blood gas analysis. Expressions of p38MAPK, NF-κB and iNOS in rat lung tissues were detected. Results Compared with Group A, expressions of p38MAPK, NF-κB and iNOS were significantly increased (P <0.01 orP< 0.05) , the protein content and the ratio of neutrophiles in BALF, contents of MDA and serum NO were significantly higher (P<0.01 or P<0.05) in Groups B, C, D and E. There was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in Groups B, C, D and E (P<0.01 or P<0.05). Compared with Group B, the expressions of p38MAPK, NF-κB and iNOS were significantly lower, and the protein content and the ratio of neutrophiles in BALF, contents of MDA and serum NO were significantly decreased, while the levels of arterial bicarbonate and partial pressure of oxygen were significantly higher in Groups C, D and E (P<0.05 or P<0.01). Compared with Group B, the lunginjury of Groups C, D and E was significantly alleviated, while there was no statistical difference among Groups C, D and E in the indices of lung injury. Conclusion Protective effect of RPR on LPS-induced ALI is closely related to the inhibited expressions of p38MAPK, NF-κB and iNOS.
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<p><b>OBJECTIVE</b>To investigate the absorption of gnsenoside Rg1 and Rb1 in Radix Gngseng at different intestine segments of rats and the influence of the drug solution concentration, pH, P-gp inhibitor.</p><p><b>METHOD</b>The intestine cannulation was performed for in situ recirculation. Gnsenoside Rg1, Rb1 and phenol red concentration in the flux were separately measured by the reversed phase HPLC and UV.</p><p><b>RESULTS</b>When the concentration was raised from 0.075-0.75 g L(-1) and 0.03-0.3 g L(-1), the uptake of ginsenoside Rg1 and Rb1 was separately linearly increased (r >0.999), and no changes of K(a) absorption fraction and t(1/2) are found. The pH of flux has no effect on drug absorption. Ginsenoside Rg1 can be absorbed in the whole intestine and no changes of K(a), absorption fraction and t(1/2) refound and all the parameters of ginsenoside Rb1 at jejunum are higher than that at ileum and duodenum (P <0. 5). Further more, P-gp inhibitor verapamil has obvious effect on the intestinal absorption of ginsenoside Rb1 (P <0.5) while it has no effect on ginsenoside Rg1.</p><p><b>CONCLUSION</b>The absorption of ginsenoside Rg1 and Rb1 in intestine of rat are first-order kinetics, the absorption mechanism is infered the passive diffusion. Ginsenoside Rg1 has no specific absorption locus in intestine of rat and ginsenoside Rb1 has specific absorption locus of jejunum. Meanwhile, ginsenoside Rb1 is the P-gp substrate, and could increase its fraction of bioavailability by corporation with P-gp inhibitor.</p>
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Animals , Female , Rats , Drugs, Chinese Herbal , Pharmacokinetics , Ginsenosides , Pharmacokinetics , Intestinal Absorption , Intestines , Physiology , Models, Animal , Panax , Chemistry , Rats, WistarABSTRACT
OBJECTIVE:To probe into effect of atorvastatin on Intima-media thickness and endothelial function.METHODS:60 patients with mild and moderate angiostegnosis of internal carotid were randomized into control group (n=30,0.1 g aspirin enteric-coated tablet) and experiment group (n=30,0.1 g aspirin enteric-coated tablet and 20 mg atrovastatin).Intima-media thickness and endothelial function were compared in 3 and 6 months before vs.after treatment.RESULTS:As compared with before treatment,intima-media thickness and endothelial function in experiment group were improved significantly after treatment (P0.05).CONCLUSION:Atorvastatin shows good effect on,improvement of intima-media thickness endothelial function,stabilization of atheromatous plaque,delay and prevention of angiostegnosis.