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1.
Article in Chinese | WPRIM | ID: wpr-965254

ABSTRACT

@#The chemical constituents of solid rice culture of the endophytic fungus Aspergillus sp.Dq-25 from barnacle were isolated and purified by silica gel, Sephadex LH-20, C18 reversed silica gel column chromatography and recrystallization.Their structures were identified by the physical and chemical properties, and by various spectroscopic methods.Six compounds were isolated and identified as: demethyldihydropenicillic acid (1), dihydropenicillic acid (2), penicillic acid (3), fortisterol (4), 22E, 24R-3P, 5a-dihydroxyerogosta-7, 22-diene-6-one (5), and (22E, 24R)-ergosterol-7, 22-diene-3β, 5α, 9α-triol-6-one (6).Compound 1 was a new butyrolactone.MTT method was used to analyze cytotoxicity, and the result showed that compound 3 exhibited inhibitory activity on five cell lines, including K562, HeLa, SGC-7901, A542 and BEL-7402, with IC50 values of 38.0 ~ 105.0 μmol/L.

2.
China Pharmacy ; (12): 677-680, 2019.
Article in Chinese | WPRIM | ID: wpr-817073

ABSTRACT

OBJECTIVE: To analyze the difference of volatile oil components from the leaves of Clausena lansium and Clausena excavata. METHODS: The volatile oil was extracted from the leaves of C. lansium and C. excavata by steam distillation. GC-MS method was adopted to analyze volatile oil to obtain TIC. After mass spectra scanning of the chromatographic peaks in the TIC diagram by HPMSD chemical workstation, chemical components of volatile oil in 2 kinds of samples were identified by retrieving and comparing mass spectrum database NIST Version 1.7. The peak area normalization method was used to calculate the relative mass fraction of each component. RESULTS: A total of 43 and 31 kinds of components were identified in volatile oil from the leaves of C. lansium and C. excavata; total relative mass fractions were 97.59% and 98.57%. Relative mass fractions of 19 and 18 components in volatile oil from the leaves of C. lansium and C. excavata were more than 1%, mainly being sesquiterpenoids. Relative mass fractions of 7 and 5 components in volatile oil from the leaves of C. lansium and C. excavata were more than 5%; the volatile components in volatile oil from the leaves of C. lansium were mainly (-)-spatol (12.35%) and (E)-5-{(1R,3R,6S)-2,3-dimethyltricyclic [2.2.1.02,6] heptane-3-yl}-2-methyl pentane-2-enol (14.70%); those from the leaves of C. excavata were mainly (E)-sesquihydrated betuline (24.94%) and 1-(1, 5-dimethy-4-hexenyl)-4-methyl-benzene (16.15%). A total of 4 components were found in volatile oil from the leaves of C. lansium and C. excavata, mainly being α-humulene, (E)-5-{(1R,3R,6S)-2,3-dimethyltricyclic [2.2.1.02,6] heptane-3-yl}-2-methylpentaeryl-2-enol, caryophyllene oxide and (-)-spatol; the content differences of them were not significant. CONCLUSIONS: The components of volatile oils from the leaves of C. lansium and C. excavata are basically similar However, the composition and comtent of specific components are quiet different and can not substituted for each other.

3.
China Pharmacy ; (12): 518-522, 2019.
Article in Chinese | WPRIM | ID: wpr-817098

ABSTRACT

OBJECTIVE: To isolate and identify the coumarins from the seeds of Clausena lansium, and to study their inhibitory activity of α-glucosidase and nematicidal activity against Panagrellus redivivus. METHODS: Column chromatography, reversed phase silica gel column chromatography and HPLC method were used to separate and purify the coumarins from the seeds of C. lansium. The structures of compounds were identified according to physicochemical properties, 1H-NMR and 13C-NMR spectral data. Using acarbose and avermectin as positive control, PNPG and Berman funnel methods were used to investigate the α-glucosidase inhibitory activity and nematicidal activity against P. redivivus, respectively. RESULTS: Seven coumarins compounds were isolated from the seeds of C. lansium, and were identified as 7-hydroxy-1-benzopiran-2-one (Ⅰ), Wampetin (Ⅱ), Lansiumarin-C (Ⅲ), Claucoumarin A (Ⅳ), Clausenalansimin A (Ⅴ), (E,E)-8-(7-hydroxy-3,7-dimethylocta-2,5-dienyloxy) psoralen (Ⅵ), Dihydroindicolactone (Ⅶ). Under 0.25 mg/mL, the α-glucosidase inhibitory rates of compounds Ⅰ, Ⅲ, Ⅴ were (32.4±1.9)%,(37.1±6.0)%, (39.5±1.1)%, respectively. Under 2.5 mg/mL, corrected mortality of compounds Ⅰ, Ⅳwere 50.5% and 47.9%. CONCLUSIONS: Compounds Ⅰ, Ⅲ, Ⅴ show α-glucosidase inhibitory activity, and compounds Ⅰ,Ⅳ display nematicidal activity against P. redivivus.  α-Glucosidase inhibitory activity of compounds Ⅲ, Ⅴ, and nematicidal activity of compound Ⅳ are found for the first time.

4.
Chinese Journal of Neurology ; (12): 21-27, 2018.
Article in Chinese | WPRIM | ID: wpr-710928

ABSTRACT

Objective To explore the relationship between asymmetrically hypointense veins (AHVs) on susceptibility-weighted imaging (SWI) and collateral circulation.Methods We retrospectively enrolled acute ischemic stroke patients with severe stenosis or occlusion of M1 segment of the middle cerebral artery ± intracranial internal carotid artery.All the patients underwent diffusion-weighted imaging (DWI),SWI,and computed tomography angiography (CTA) of intracranial and cervical arteries within 72 hours from symptom onset.We explored the association of the level of AHVs with the degree of the regional leptomeningeal score (rLMC) on baseline CTA and other clinical and image data.The factors that might influence the prognosis of stroke were also analyzed.Results Fifteen patients with mild AHVs and 15 with extensive AHVs were enrolled in our study.The level of AHVs was positively correlated with CTA rLMC (r =0.481,P =0.007) and the degree of collateral circulation (r =0.402,P =0.028).Patients with extensive AHVs had better collateral status,smaller DWI infarction lesion ((11.62 ± 9.07) ml vs (95.77 ± 91.12) ml,t =3.559,P =0.001),and lower NIHSS scores on admission (6.47 ± 4.34 vs 12.33 ± 7.60,t =2.595,P =0.015)and at discharge (4.80 ± 4.69 vs 9.60 ± 7.03,t =2.200,P =0.036).The high degree of rLMC,small DWI lesion,young age and lower NIHSS scores,but not extensive AHVs were related with favorable outcome at 3 months after stroke.Conclusion Extensive AHVs can reflect good collateral circulation to some extent,but cannot be equivalent to or replace the collateral status.

5.
Chinese Journal of Neurology ; (12): 712-716, 2018.
Article in Chinese | WPRIM | ID: wpr-711010

ABSTRACT

Objective To investigate the frequency and location of cerebral microbleeds in cerebral autosomal dominant arteriopathy with subcortical infarct and leukoencephalopathy (CADASIL) to understand the imaging and clinical features of the disease.Methods Cranial magnetic resonance imaging and susceptibility-weighted imaging were assessed in seven symptomatic CADASIL patients in People's Hospital of Zhengzhou University from 2014 to 2017.Imaging features and clinical significance of these patients were analyzed retrospectively.Results The seven patients were diagnosed by Notch3 gene detection.Mutations were found in exon 11 in four cases,and in exon 4 in three cases.All the seven patients with CADASIL had cerebral microbleeds,the number of which was 108 (4-36).The number of cerebral microbleeds was found to be higher in cortico-subcortical region than in any other regions.One of CADASIL patients with cerebral microbleeds had intracerebral hemorrhage located in external capsule.The patient with intracerebral hemorrhage had hypertension and multiple cerebral microbleeds.Conclusions Cerebral microbleeds are common imaging characteristics in symptomatic CADASIL,most of which locate in cortico-subcortical region.Cerebral hemorrhage is one of the clinical manifestations of CADASIL patients.

6.
Chinese Journal of Neuromedicine ; (12): 1218-1224, 2017.
Article in Chinese | WPRIM | ID: wpr-1034712

ABSTRACT

Objective To explore the predictive value of susceptibility vessel sign (SVS) in clinical outcomes of patients with acute anterior circulation ischemic stroke. Methods The clinical and imaging data of patients with acute anterior circulation ischemic stroke, admitted to our hospital from January 2013 to April 2015, were prospectively collected. All of them underwent diffusion-weighted imaging (DWI), susceptibility-weighted imaging (SWI), and magnetic resonance angiography (MRA) within 72 h of symptom onset. According to the results of the examination, the presence, location and length of SVS, DWI lesion volumes, degrees of vascular stenosis, perfusion status of distal blood flow and vascular stenosis sites were assessed and recorded. The prognoses of the patients were evaluated by modified Rankin scale (mRS) 3 months after stroke. The univariate analysis and multivariate Logistic regression analyses were used to explore the independent risk factors for poor prognosis. Results One hundred and sixteen patients were enrolled in this study. There were 43 patients in the SVS(+) group and 73 patients in the SVS(-) group. As compared with patients from the SVS(-) group, patients from SVS(+) group had higher systolic pressure, lower thrombolysis in cerebral infarction scale (TICI) scores, larger infarction volume, higher NIHSS scores on admission and lower percentage of favorable outcome, with statistically significant differences (P<0.05). Of the 116 patients, 40 had poor outcome while 76 achieved favorable prognosis;as compared with the patients with favorable outcome, those with poor outcome had higher percentage of the elderly, higher percentages of atrial fibrillation and SVS, lower TICI scores, larger infarction volumes, and higher NIHSS scores on admission, with statistically significant differences (P<0.05). Multivariate Logistic regression analysis revealed that NIHSS on admission (OR=1.278, 95%CI:1.145-1.426, P=0.000), age (OR=1.085, 95%CI: 1.036-1.136, P=0.001), and presence of SVS (OR=3.390, 95%CI: 1.122-10.240, P=0.030) were independent parameters to predict poor prognosis. For patients with SVS(+) status, 18 patients achieved favorable outcome, while 25 had poor outcome;patients with poor outcome had larger infarction volumes on DWI, higher NIHSS scores on admission, higher rate of SVS located at the proximal M1 and longer SVS, with statistically significant differences (P<0.05). On multivariate Logistic regression analysis, NIHSS score on admission was an independent predictor of poor outcome (OR=1.341, 95%CI: 1.123-1.602, P=0.001). Conclusion The presence of SVS is an independent predictor for poor clinical outcome in patients with acute anterior circulation ischemic stroke.

7.
Chinese Journal of Neurology ; (12): 822-825, 2017.
Article in Chinese | WPRIM | ID: wpr-665131

ABSTRACT

Objective To analyze the clinical presentation , the mutation of the pathogenic genes and imaging features in a Chinese Han early-onset Alzheimer's disease pedigree.Methods A pedigree of Alzheimer's disease was collected.The DNA sequence of presenilin 1 (PSEN1), presenilin 2, micro-tubule associated protein tau ,β-amyloid precursor protein gene was analyzed , the clinical presentation , results of accessory examination , neuropsychological evaluation of the proband were investigated and the point mutations of some members of the family , 50 sporadic Alzheimer's disease patients , 50 normal controls were verified.Results The proband of the family appeared as language impairment , memory loss, personality change, repeated language, visuospatial impairment, mental and behavior disorder.The gene detection showed p.L226R mutation in the condon 226 in the exon 7 of PSEN1 gene of the proband and five other family members (Ⅲ1 ,Ⅲ2 ,Ⅲ4 ,Ⅲ6 ,Ⅲ7 ).The mother of the proband had the suspicious symptoms , and the sister and the brother of the proband had the similiar symptoms with the proband , all of whom died.Fifty sporadic Alzheimer'disease patients and 50 unrelated normal subjects did not have the mutation .The computed tomographic angiography showed that the brain blood vessels were normal and 18 F-fludeoxyglucose positron emission tomography (18F-FDG-PET) showed brain atrophy and hypometabolism in frontotemporal regions, parietal regions, hippocampal areas, however, the MRI, MRA and 18F-FDG-PET of the two mutation carriers (Ⅲ6 ,Ⅲ7 ) were all normal.Conclusion We reported a novel mutation in an early-onset Alzheimer's disease family presented as language impairment in the early stage of the disease , the p.L226R mutation of PSEN1, which may be a pathogenic mutation to cause the family's dementia.

8.
Acta Pharmaceutica Sinica ; (12): 1688-91, 2013.
Article in English | WPRIM | ID: wpr-445451

ABSTRACT

To investigate the chemical constituents of the endophytic fungus Penicillium sp. FJ-1 of Ceriops tagal, the chemical constituents were isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were elucidated on the basis of spectroscopic analysis. Their antibacterial activity was tested by paper disco diffusion method. Two compounds were isolated and identified as 7-hydroxy-deoxytalaroflavone (1), and deoxytalaroflavone (2). Compound 1 is a new compound, and compounds 1 and 2 showed weak activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.

9.
Article in English | WPRIM | ID: wpr-819492

ABSTRACT

OBJECTIVE@#To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a hospital.@*METHODS@#Phenotype of the isolate was investigated by conventional microbiological methods, including Gram-staining, colony morphology, tests for haemolysis, catalase, coagulase, and antimicrobial susceptibility test. The mecA and 16S rRNA genes were amplified by the polymerase chain reaction (PCR) and sequenced. The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by phylogenetic analysis and multiple sequence alignment.@*RESULTS@#The isolate in this study was a gram positive, coagulase negative, and catalase positive coccus. The isolate was resistant to oxacillin, methicillin, penicillin, ampicillin, cefazolin, ciprofloxacin erythromycin, et al. PCR results indicated that the isolate was mecA gene positive and its 16S rRNA was 1 465 bp. Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus, and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the GenBank.@*CONCLUSIONS@#16S rRNA gene sequencing is a suitable technique for non-specialist researchers. Laboratory animals are possible sources of lethal pathogens, and researchers must adapt protective measures when they manipulate animals.


Subject(s)
Animals , Mice , Base Sequence , Drug Resistance, Multiple, Bacterial , Microbiology , Microbial Sensitivity Tests , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S , Genetics , Staphylococcus haemolyticus , Classification
10.
Article in Chinese | WPRIM | ID: wpr-586395

ABSTRACT

Objective To investigate the composition and cytotoxic activity of the organic acids extracted from a red alga Gracilaria lemaneiformis.Methods Growth inhibition rate of the extract on HeLa cell was determined by MTT assay.The composition and contents of the organic acids were analysed by GC-MS after methylied.Results The organic acids from Gracilaria lemaneiformis showed potent cytotoxic activity against HeLa cell,IC_(50)=6.5?g? mL~(-1).Thirteen compounds were characterized from methylied organic acids.The major constituent was palmitic acid methyl ester (61.34%).Their relative acids were mainly C_(16)or C_(18)fatty acids and phthalates.Of them,palmitic acid,stearic acid,palmitoleic acid,oleic acid and linoleic acid have been reported to show antitumor activity.Conclusion The fatty acids mentioned above maybe a part of antitumor components of Gracilaria lemaneiformis.

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