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1.
Article in Chinese | WPRIM | ID: wpr-931102

ABSTRACT

Objective:To explore the effect of knockdown of the homeobox gene paired-box 6 ( Pax6) on the biological behavior and epithelial-mesenchymal transition (EMT) of human lens epithelial cells (LECs). Methods:The SRA01/04 human LECs were divided into small interfering RNA-Pax6 (siRNA-Pax6) group transfected with siRNA-Pax6 and siRNA negative control (siRNA-NC) group transfected with disordered siRNA.Cell survival rate was detected by cell counting kit-8 method at 24, 48 and 72 hours after transfection.Cell cycle distribution and apoptosis were analyzed by flow cytometry at 48 hours after transfection.Migratory capability of cells was examined by cell scratch test at 24 hours after transfection.The mRNA relative expression levels of Pax6, α-crystallin A (CRYAA), α-crystallin B (CRYAB), Sox2, α-smooth muscle actin (α-SMA) and E-cadherin were detected by quantitative real-time PCR at 48 hours after transfection.The relative expression of Pax6 protein was detected by Western blot at 48 hours after transfection.Results:There was a significant difference in cell survival rates at different time points between the two groups ( Fgroup=4.776, P<0.05; Ftime=13.535, P<0.05). The cell survival rate of siRNA-Pax6 group was obviously lower than that of siRNA-NC group at 48 and 72 hours after transfection, and the differences were statistically significant (both at P<0.05). Compared with siRNA-NC group, the proportion of cells in G 0/G 1 phase was significantly increased and the proportion of cells in S phase was significantly reduced in siRNA-Pax6 group ( t=9.971, -5.063; both at P<0.05). The cell migration rate of siRNA-Pax6 group was (19.73±6.07)%, which was lower than (70.56±2.97)% of siRNA-NC group, showing a statistically significant difference ( t=-7.245, P<0.05). The relative expressions of Sox2 mRNA and α-SMA mRNA were lower, and the relative expression of E-cadherin mRNA was higher in siRNA-Pax6 group than siRNA-NC group, with statistically significant differences between them ( t=-23.254, -5.294, 6.062; all at P<0.01). The relative expression of CRYAA mRNA and CRYAB mRNA was significantly higher in siRNA-Pax6 group than siRNA-NC group, and the differences were statistically significant ( t=5.521, 8.270; both at P<0.01). The relative expressions of Pax6 mRNA and protein in siRNA-Pax6 group were 0.27±0.01 and 0.24±0.05, respectively, which were both lower than 1.00±0.05 and 1.14±0.10 in siRNA-NC group, showing statistically significant differences ( t=-14.456, -4.458; both at P<0.001). Conclusions:Silence of Pax6 can suppress the proliferation and EMT of human LECs and enhance the expression of crystallin.

2.
Chinese Journal of Orthopaedics ; (12): 872-880, 2021.
Article in Chinese | WPRIM | ID: wpr-910669

ABSTRACT

Objective:To analyze the prevalence and distribution characteristics of diffuse idiopathic skeletal hyperostosis (DISH) in hospitalized patients with thoracic ossification of the ligamentum flavum (TOLF).Methods:The clinical records of 132 consecutive TOLF patients from January 2018 to June 2019 were retrospectively studied. DISH was identified by the preoperative X-ray and CT and its prevalence was calculated. The prevalence of patients with different genders, different age groups and different ossification types was compared. The segmental distribution of DISH and the distribution in the upper (T 1-T 4), middle (T 5-T 8), and lower thoracic spine (T 9-T 12) were analyzed. Ossification degree of DISH was evaluated based on the Meta scoring system. The demographic characteristics (age, gender, BMI, etc.) were compared between DISH and non-DISH group. Results:Forty-nine patients was diagnosed as DISH with the prevalence of 37.1% in all included cases. The prevalence was about twice as high in male (46.3%) than in female (23.1%) ( χ2=8.806, P=0.003). The prevalence in the age groups of <40, 40-49, 50-59, 60-69 and ≥70 years was 20.0%, 28.0%, 34.4%, 44.0%, and 66.7%, respectively. The prevalence in long-segment TOLF patients (45.1%) was significantly higher than that in short-segment TOLF patients (24.0%) ( χ2=5.937, P=0.015). DISH most frequently affected T 8,9 levels (91.8%). The total number and mean number of ossified segments were 365 and 7.4, respectively. Ossification lesions in the upper, middle, lower thoracic spine accounted for 26.03%, 40.54%, and 33.15%, respectively. Grade I, grade II, and grade III ossification accounted for 21.4%, 28.5% and 50.1%, respectively. The mean age of the DISH group was older than the non-DISH group ( t=2.024, P=0.045). The proportion of male patients in the DISH group was significantly higher than that in the non-DISH group ( χ2=8.806, P=0.003). The average height and weight in the DISH group were significantly greater than those in the non-DISH group ( t=2.564, P=0.012; t=2.191, P=0.030), whereas no significant differences in BMI and constituent ratio of concurrent diabetes, cardiac disease, hypertension between two groups were observed. Conclusion:The prevalence of DISH in patients with TOLF is 37.1%. Male, elderly and long-segment TOLF patients are associated with higher prevalence. DISH frequently occurs in the middle and lower thoracic spine, and T 8,9 is the common affected segment. Ossification lesions may develop with age. Demographic characteristics of DISH group differ, to some extent, from those of non-DISH group.

3.
Article in Chinese | WPRIM | ID: wpr-908290

ABSTRACT

Objective:To explore the peer support experience of newly graduated nurses during standardized training period.Methods:Using phenomenological qualitative research methods and purposive sampling, from April to December 2020, 32 new nurses who participated in the standardized training peer support program in the Affiliated Hospital of Yangzhou University were selected. Semi-structured interview was conducted and Claizzi content analysis method was used to analyze the data.Results:Peer support during the standardized training of new graduated nurses could be summarized into four major themes and eleven sub-themes. Theme 1: cognition and attitude (emotional security, adaptive guidance, recognition of the value of peer support, positive intention to support). Theme 2: peer support needs (department-related information needs; knowledge and skills experience needs; emotional communication needs; career growth support needs). Theme 3: peer support experience (adaptive behavior changes, cognitive and emotional changes, differences in perceived expectations of supporters and support effects).Conclusions:During the standardized training, we should attach importance to the important supporting role of peer support for new nurses. By building a peer support platform for new nurses and broadening experience exchange channels, we can enhance the positive experience of peer support, so as to promote the role adaptation and career growth of new nurses.

4.
Article in Chinese | WPRIM | ID: wpr-911443

ABSTRACT

Objective:To study clinical characteristics and pregnancy outcomes under anti-coagulation therapy of non-criteria obstetric antiphospholipid syndrome.Methods:Patients suspected of obstetric antiphospholipid syndrome(OAPS) were recruited through Chinese Rheumatism Data Center from 2015 to 2019 consecutively. Patients fulfilling 2006 Sydney revised antiphospholipid syndrome criteria were classified as OAPS. Patients fulfilling definition of non-criteria OAPS(NCOAPS) by expert consensus on diagnosis and management of obstetric antiphospholipid syndrome of China were classified as NCOAPS. Clinical characteristics and laboratory results of two groups were compared. Live birth rates and pregnancy outcomes under anti-coagulation therapy were studied.Results:A total of 88 patients were enrolled, including 56 patients (63.6%) as OAPS, 32(36.4%) as NCOAPS. Live births were only reached in 16.1% (9/56) in OAPS patients and 12.5%(4/32) in NCOAPS. Fetal losses after 10 weeks of gestation and pre-eclampsia before 34 weeks were more common in OAPS group compared to NCOAPS group [78.6%(44/56) vs. 18.8%(6/32), P<0.001; 25.0%(14/56) vs. 3.1%(1/32), P=0.020, respectively]. After enrollment, 15 pregnancies were recorded in OAPS, 10 in NCOAPS, all of whom were treated with low-dose aspirin (LDA) combined with low-molecular weight heparin (LMWH). Live birth rates saw dramatic improvements compared to baseline levels in OAPS [16.1% (9/56) vs. 11/15] along with NCOAPS [12.5% (4/32) vs. 7/10]. Conclusion:Though NCOAPS and OAPS patients differ in antiphospholipid antibody spectrum and pattern of pregnancy morbidities, both groups benefit from LDA combined with LWMH treatment, as live birth rates improve. Non-criteria OAPS patients are recommended to receive anti-coagulation therapy during pregnancy.

5.
Chinese Journal of Orthopaedics ; (12): 1493-1502, 2020.
Article in Chinese | WPRIM | ID: wpr-869098

ABSTRACT

Ossification of posterior longitudinal ligament (OPLL) and ossification of ligamentum flavum (OLF) are clinically common heterotopic ossification diseases, which are the main causes of cervical and thoracic spinal stenosis and spinal cord injury. In some cases, OPLL and OLF may involve multiple spinal sites, individually or concurrently, increasing the difficulty of clinical diagnosis and treatment. This review initially attempts to define this specific ossification phenomenon as Tandem ossification of intraspinal ligaments (TOIL). It refers to a kind of severe spinal ligament ossification diseases caused by multiple factors, that OPLL and OLF occur alone or in combination at two or more sites of the cervical, thoracic, or lumbar spine with five or more ossified segments, progressively compressing the spinal cord or nerves, thereby resulting in a series of complex clinical symptoms. The prevalence of TOIL remains unknown, but its clinical detection rate is relatively high, and the most common TOIL is cervical OPLL combined with thoracic OPLL or/and OLF. Moreover, occurrence and development of TOIL involve many factors and its definitepathogenesis is not clear. Damage of upper and lower motor neurons can occur simultaneously in TOIL patients so that their clinical symptoms and signs often interfere with each other. TOIL has various forms, complex imaging features, and no uniform diagnostic and localization diagnostic criteria, which may easily lead to missed diagnosis and misdiagnosis. It is recommended that TOIL is identified by the typical symptoms and signs as the basis, CT three-dimensional reconstruction and MRI signs as the reference, and combination of the possible risk factors and clinical experience. Currently, surgical decompression for responsible lesions is the only effective treatment for symptomatic TOIL. However, domestic and foreign scholars still have great arguments on the optimal surgical strategy of TOIL. No consensus has been reached on how to determine the operating segment and the extent of decompression, which surgical approach and technique to be adopted, and when to select staged or one-stage combined surgery. Therefore, this review summarizes and discusses the current status and progress of the clinical characteristics and surgical strategies of TOIL through searching the relevant literature, to provide a reference for clinicians to diagnose and treat TOIL.

6.
Chinese Journal of Orthopaedics ; (12): 962-970, 2020.
Article in Chinese | WPRIM | ID: wpr-869047

ABSTRACT

Thoracic ossification of ligament flavum (TOLF) is a pathological process of heterotopic bone formation from ligamentum flavum tissue, which is the most common cause of thoracic spinal stenosis and thoracic myelopathy. Imaging examination is the predominant measure for localization and qualitative diagnosis for TOLF. Various TOLF classification systems have been reported currently, based on its morphology, distribution, configuration of compressed spinal cord or its association with peripheral tissue structures. However, there has been no unified classification due to confusing clinical applications. Therefore, we conducted a review to summarize TOLF classification systems and to evaluate the reliability of these classification systems and the diagnostic accuracy of various imaging modalities. As demonstrated in literatures, the diagnostic accuracy of radiography and the reliability of related classification were low. CT-based Sato classification (lateral, extended, enlarged, fused, tuberous) and Kuh classification (unilateral, bilateral, bridging), MRI-based Kuh classification (round, beak) and Chen Zhongqiang classification (isolated, skipping, continuous), Muthukumar classification combined with dural ossification (Tram track sign, Comma sign) elaborated ossification characteristics from different angles and dimensions. These classification systems were clinically significant in pathogenesis implication, risk assessment, treatment guidance and prognosis judgement, which showed high diagnostic accuracy and reliability. Combining multi-dimension and multi-level parameters of CT and MRI will play an important role in the diagnosis, treatment and new classification research of TOLF.

7.
Chinese Journal of Orthopaedics ; (12): 381-388, 2020.
Article in Chinese | WPRIM | ID: wpr-868979

ABSTRACT

Distal junctional problem (DJP) is one of the severe complications after spinal correction, fixation and fusion. As the number of patients receiving spinal surgery increased recently, the incidence of DJP also increased dramatically. Compared with proximal junctional problem, the incidence of DJP is low. However, the clinical symptoms are severe, and the rate of surgical revision is high in patients with distal junctional problems. DJP include distal junctional kyphosis (DJK) and distal junctional failure(DJF). The definition of DJK is confusing, however, and the most commonly used was that the distal junction Angle at the last follow-up was greater than 10° and increased by 10° compared with that before surgery. There are 6 DJF modes: progressive loss of lumbar lordosis,acute wedging in the disc below the instrumentation, fracture of LIV, osteoporotic fracture below the long rigid fixation, failure of the instrumentation at LIV, spinal stenosis and or segmental instability underneath the instrumentation. Possible risk factors for DJP include weight, age, type of spinal deformity, osteoporosis, choice of LIV, hip disease, deformity location, surgical approach, surgical procedure, fusion segments, fixation devices, LIV at L5, fixed to S1 with no iliac screws, poor restoration of spinal alignment, et al. Currently, there are some controversies in DJP, mainly including the incidence, risk factors whether needs to and how to revise. The review intends to conduct a simple literature review of the current DJP diagnostic criteria, incidence, risk factors, and other research progress, in order to improve the understanding of the distal junction problem.

8.
Chinese Journal of Plastic Surgery ; (6): 1226-1229, 2019.
Article in Chinese | WPRIM | ID: wpr-800213

ABSTRACT

Objective@#To investigate the clinical outcomes of snuffbox perforator flap of radial artery to repair large soft tissue defects of the thenar and dorsal thumb.@*Methods@#From June 2012 to March 2017, a total of 18 cases with large soft tissue defects of the thenar and dorsal thumb were hospitalized in the Second Hospital of Tangshan. There were 13 males and 5 females, with an average age of 39 years (range, 17 to 64 years). The right hand was involved in 14 cases and the left in 4 cases. There were 5 cases of thenar defect, 7 cases of dorsal thumb defect and 6 cases of thenar combined with dorsal thumb defect. The tendon and / or bone were exposed in all 18 cases. The size of the wounds after debridement ranged from 2.5 cm × 3.5 cm to 4.5 cm × 6.0 cm. The snuffbox perforator flap was designed to repair the defect based on the pivot point of snuff box and the axis line from styloid process of radius to capitulum of radius. Four cases with extensor tendon defects of the thumbs were grafted with a section of brachial radial tendon. The dimension of the flaps ranged from 2.8 cm × 3.8 cm to 5.0 cm × 6.8 cm. The observation indexes included blood supply and appearance of the flap, the total activity of flexion and extension (TAM) of the injured thumb, the angle of the first web span and the satisfaction of the patients.@*Results@#All flaps survived uneventfully. The donor area of forearm was directly closed in 16 cases, and the remaining wound in 2 cases was repaired by free skin graft. All the cases were followed up for 8 to 16 months (mean, 12 months). The good appearance of the flaps with soft texture was obtained. The average values of TAM of the injured thumbs and the first webs were 139°and 96°, respectively. They were excellent in 15 cases and good in three cases for TAM, and excellent in all cases for the first webs. 10 patients were very satisfactory with flap appearance and thumb function and the rest was satisfactory.@*Conclusions@#The snuffbox perforator flap of radial artery provides a reliable and simple technique with constant blood supply and minimal donor-site cost, which could be an ideal option for repairing large soft tissue defects of the thenar and dorsal thumb with extensor tendon defects of the thumbs.

9.
Article in Chinese | WPRIM | ID: wpr-808506

ABSTRACT

Objective@#To compare the clinical effect of innervated reverse digital artery island flap and the digital artery island flap with dorsal branches in the repair of fingertip defects.@*Methods@#From January 2014 to June 2015, a total of 43 cases (50 fingers) with fingertip defects in 2-5 fingers were randomly treated with reverse digital artery island flaps (23 cases) or the digital artery island flaps with dorsal branches (20 cases). All cases underwent emergency surgery. The operative time, patient satisfaction of appearance for the reconstructed fingers, the static 2-point discrimination of the flaps, active motion of the injured finger joints and postoperative appearance on donor site were compared respectively.@*Results@#All flaps survived primarily. The follow-up period ranged from 12-18 months. No significant differences were found in operative time and active motion of the injured finger joints between the two groups (P>0.05). Patient satisfaction of appearance and the static 2-point discrimination of the flaps in reverse digital artery island flap group were better than those in the digital artery dorsal branch island flap group (P<0.05). For the donor site, the texture of donor site in reverse digital artery island flap was soft without significant Scar contracture and pigmentation. There were 5 cases with mild scar contracture in donor site at the digital artery island flap with dorsal branches.@*Conclusions@#The reverse digital artery island flap and the digital artery island flap with dorsal branches are all suitable for the repair of fingertip defects. Compared with the digital artery island flap with dorsal branch, the reverse digital artery island flap can provide better fingertip sense and appearance.

10.
Chinese Journal of Microsurgery ; (6): 540-543, 2017.
Article in Chinese | WPRIM | ID: wpr-665837

ABSTRACT

Objective To investigate the clinical effectiveness of the reversed flaps pedicled with lateral vas-cular chain of dorsal branch of digital artery and perforating branch of the dorsal metacarpal artery for relaying repair of soft tissue defects at middle-distal part of the 2nd ~ 5th finger and donor site. Methods From June, 2014 to May, 2016, a total of 18 cases (11 male and 7 female) with soft tissue defects at middle-distal part of 2nd~5th fin-ger were treated with the reversed flap pedicled with lateral vascular chain of dorsal branch of digital artery. The donor site was repaired with the reversed flap pedicled with the perforating branch of the dorsal metacarpal artery. The aver-age age at the time of reconstruction was 41 years. The right hand was involved in 12 cases and the left in 6 cases. The etiologies of injury were avulsion (n=5) , crush (n=6), and saw-cut injury (n=7). There were 15 cases with soft tissue defect at the distal phalanx and 3 cases with loss of distal and middle phalanx. All these cases were exposed with bone or tendon. The defect size ranged from 1.0 cm × 1.8 cm to 2.0 cm × 3.0 cm. And the dimension of the re-versed flaps pedicled with lateral vascular chain of dorsal branch of digital artery and perforating branch of the dorsal metacarpal artery ranged from 1.2 cm × 2.0 cm to 2.3 cm × 3.3 cm, and 1.4 cm × 2.3 cm to 2.6 cm × 3.6 cm, respec-tively. Postoperative follow-up was done termly. Results All flaps survived uneventfully with primary healing at donor site. The mean of followed-up time was 11 months (ranged from 6 to 15 months). The appearance and texture of the flaps were satisfactory and similar to the surrounding tissue. There was only some linear scar left at the dorsum of the palm. On the basis of the MHQ, 16 patients were strongly satisfied (score 5) with the appearance of the flap, and the remaining 2 patients was satisfied (score 4) with the appearance. The static 2-point discrimination of the flaps at the middle-distal part of 2nd~5th finger was 5-9 mm. Based on TAM assessment, the function of the injured finger was graded as excellent in 15 cases, good in 3 cases. Conclusion The relaying reversed flaps pedicled with lat-eral vascular chain of dorsal branch of digital artery and perforating branch of the dorsal metacarpal artery can be applied for repair of soft tissue defects at middle-distal part of 2nd~5th finger and donor site with easy procedure, reliable blood supply, satisfactory results, good appearance as well as less complications at donor sites.

11.
Article in Chinese | WPRIM | ID: wpr-467807

ABSTRACT

Objective To investigate the effect of Euryale ferox seeds ethanol extract on renal funtion of diabetic nephropathy (DN) induced by strepotozotocin (STZ) in rats and the antioxidant ability of ethanol extract,water extract supernatant and precipitation. Methods Ethanol extract of Euryale ferox seeds were prepared by ethanol extraction and followed by evaporation and freeze-dry. The seeds residue was further extracted with water and precipitated with ethanol to obtain water extract supernatant and precipitation. Sprague Dawley male rats were given intraperitoneal injection of STZ at a dose of 60 mg/kg to induce DN. STZ-induced DN rats were administrated by gavage with different dose of Euryale ferox seeds ethanol extract(75, 150, 300 and 600 mg/kg body weight) once daily for 12 weeks. 24 h urine was collected for examining urinary protein and microalbumin every four weeks. Blood serum was also collected for examining of serum creatinine (Cr) and blood urea nitrogen (BUN) level after rats sacrificed at the end of 12 weeks after administration, and the pathological changes of kidney were observed. Antioxidant capacity of the three extracts were determined by Fe3+ reduction, and radical scavenging rate to 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazl(DPPH), O2-and OH-free radicals. Results Compared with model group, the content of 24 h urine protein and urine microalbumin of DN rats were reduced in all Euryale ferox seeds ethanol extract administration groups, and 24 h proteinuria were significantly reduced in the group of 150 and 600 mg/kg administration(P<0.05);BUN level was also reduced in all dose of Euayale ferox seeds ethanol extract administration, and more remarkably reduced in the group of 300mg/kg (P<0.01). 12 weeks after administration, kidney damage amelioration was observed in pathological sections of rats in Euryale ferox seeds ethanol extracts 300 and 600 mg/kg groups. Meanwhile, Euryale ferox seeds ethanol extract showed a certain antioxidant abilities in reducing of Fe3+ and scavenging DPPH, O2- and OH- at the concentrations of 0.6, 0.8 and 1.0 mg/ml. Conclusion Euryale ferox seeds ethanol extract could decrease proteinuria of STZ-induced DN rats,this may be associated with its antioxidant capacity.

12.
Article in Chinese | WPRIM | ID: wpr-464569

ABSTRACT

The seed, coat, petiole and peduncle of Euryale ferox(belonging to Nymphaeaceae) are used as medicinal potions through solvent extraction, reflux extraction and ultrasonic extraction, and purification on macroporous resin. The quality controls are performed by near infra-red fingerprint spectrum, HPLC, X-ray diffraction Fourier spectrum, and ITS2 barcode. The nutrients of E. ferox contain large amount of amino acids, fatty acids and various microelements; medicinal ingredients are identified to be polyphenol, sesquineolignan, tocopherol, cerebroside, and cyclic dipeptide. The pharmacological effects of E. ferox extracts include antioxidant, free radical scavenging, hypoglycemic activity, decreasing urine protein, bacteriostasis and prevention of gastric mucosal injury. Treatment with E. ferox preparations for diabetes and chronic nephritis are performed in clinics. This review summarizes the study of E. ferox in recent years for further research and development.

13.
Chinese Journal of Orthopaedics ; (12): 1368-1373, 2011.
Article in Chinese | WPRIM | ID: wpr-423303

ABSTRACT

ObjectiveTo establish a new biomechanical test for evaluating biomechanical properties of femoral metaphysis in ovariectomized rats.MethodsTwenty-five-month-old female Sprague-Dawley rats were randomly divided into ovariectomized (OVX) group and the sham-operated (Sham) group (n=10).Twelve weeks after operation,femur BMD and histomorphometry of distal femur were detected.Furthermore,femur biomechanical parameters were determined by diaphysis three-point bending test and a new designed metaphysis bending test.The relationships between the biomechanical parameters and BMD or bone histomorphometry were assessed with Pearson correlations.ResultsThe femur BMD,bone histomorphometrical indexes(%Th.Ar,Tb.N,Tb.Th),and biomechanical parameters(maximum load,yield load and stiffness) of OVX group significantly decreased compared with Sham group.In metaphysis bending test,the mean difference of the maximum load(Fmax),yield load(Fy) between group OVX and Sham were significant higher than that in diaphysis three-point bending test.Positive correlations between biomechanical parameters and femur BMD or bone histomorphometry indexes were observed in both the diaphysis bending and metaphysis bending test.The biomechanical parameters in the metaphysis bending test showed stronger correlations with BMD and bone.ConclusionFemur metaphysis bending test could be used to evaluate the biomechanical properties of osteoporosis.When compared with diaphysis bending test,femur metaphysis bending test is more sensitive in evaluating the change of biomechanical properties of femur in osteoporotic rats.

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