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1.
Chinese Journal of Immunology ; (12): 554-557, 2017.
Article in Chinese | WPRIM | ID: wpr-515353

ABSTRACT

Objective:To study performed of develop a mice model of allergic diseases induced by crude extractings from populus pollen.Methods: A total of 60 BALB/c mice were divided randomly into there groups:normal control group,Albumin Egg(OVA) group and populus pollen model group with 20 in each.Mices were repeatedly sensitized by intraperitoneal injections of OVA or crude populus pollen extract every 5 d for four doses.Five days after the last sensitization,mices were repeatedly challenged by once daily antigen from 21-25 d.The changes of inflammatory cells in bronchoalveolar lavage fluid(BALF) were stained with hematoxylin and eosin(HE) to evaluate the degree of allergic inflammation.PAS staining was used to observe the secretion of airway mucus;The changes of the nasal mucosas and lungs of mice were stained with HE to evaluate the degree of allergic inflammation.And the average optical density of IL-4 and IFN-γ positive cells in lung tissue was measured by immunohistochemistry.The total IgE in the serum was also measured by enzyme-linked immunoassay(ELISA).Results: Compared with the mice in normal control group,those in OVA group and model group developed obvious allergic inflammation in the nasal mucosas and lungs,and increased airway mucus secretion.The number of inflammatory cells including eosinophil and neutrophils markedly increased in BALF smear.The average optical density of IL-4 positive cells in lung tissue was all increased in OVA group and model group compared with those in normal control group,and the average optical density of IFN-γ in lung tissue was on the contrary.The total IgE in the serum were all increased in OVA group and model group compared with those in normal control group,and the IFN-γ in the serum was significantly reduced in OVA group and model group compared with those in normal control group.Conclusion: Taken together,crude populus pollen extract can successfully induce a mice model of allergic diseases.This model is a useful tool in studying the mechanisms of allergic disease.

2.
Article in Chinese | WPRIM | ID: wpr-503112

ABSTRACT

Objective To discuss the effects of different drying methods on composition and antioxidative activities of the volatile oil fromCymbopogon citrates; To optimize the best drying method for Cymbopogon citrates. MethodsCymbopogon citrates was dried by drying in the sun, drying in the shade and oven drying at 40℃. Volatile oil was extracted by steam distillation. Chemical constituents in the volatile oil were analyzed by GC-MS and the antioxidative activities were determined by ferric reducing antioxidant power (FRAP method).Results Extraction rate of the volatile oil fromCymbopogon citratesunder the environment of freshness, sun drying, shade drying and oven drying at 40℃ were 0.25%, 1.21%, 1.19% and 1.17%, respectively; after dried by different methods, main constituents and antioxidative activities of the volatile oil fromCymbopogon citrates were basically same. Conclusion Different drying methods have little influence on composition and antioxidative activities of the volatile oil fromCymbopogon citrates. Oven drying at 40℃ was the best way to dryCymbopogon citrates.

3.
Article in Chinese | WPRIM | ID: wpr-685720

ABSTRACT

AIM:To observe the effect of supplementing bilirubin on the anti-oxidative stress ability of red cell membrane in training rats through establishing animal models of high-intensity training. METHODS:The experiment was performed in the physiological laboratory of Physical Institute of Jiangxi Normal University from December 2004 to January 2005.①After one week of adaptive feeding,24 male SD rats were randomly divided into quiet control group,high intensity sports training and physiological saline group(training group),high intensity sports training and bilirubin group(training and supplementing group)with 8 animals in each group.②The training group and the training and supplementing group did the running on the platform with the slope of 0?at the first three weeks and 5?at the fourth week,6 days every week expect Sunday.The training and supplementing group was intragastrically infused with bilirubin by 40mg/kg body mass at 17:00-18:00 after training every day,and the other groups were given physiological saline,six times every week.Anticoagulatory blood about 2mL of heparin was harvested to prepare red cell membrane sample and measure the relevant indexes on the 4~(th)Sunday.③The serum total bilirubin level was determined using automatic biochemical analysis instrument;the malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)of red cell membrane by TBA;the activity of superoxide dismutase(SOD)of red cell membrane using the method of Xanthine oxidase.All the treatment for animals was based on the ethical standards. RESULTS:All 24 rats were involved in the result analysis.①The serum total bilirubin level in training group was lower than quiet control group(P0.05).②The MDA of the red cell membrane in the training group was higher than the control and training and supplementing group(P0.05).③The activity of SOD in training group was lower than quiet control group(P0.05),but the training and supplementing group was higher than the control group and the training group(P

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