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Article in Chinese | WPRIM | ID: wpr-270192


<p><b>OBJECTIVE</b>To investigate type I collagen metabolism in patients with arsenic poisoning in Dazhuyuan town, Ankang area in southern Shaanxi province known for high-level exposure to arsenic (As), fluoride (F) and selenium (Se).</p><p><b>METHODS</b>This case-control study involved 29 patients with arsenic poisoning from Dazhuyuan, a town in Ankang area of Shaanxi province affected by coal-borne endemic arsenicosis. Thirty individuals without arsenic poisoning from Chang'an county of Xi'an city were included as the control group. The serum concentrations of As and Se were determined using a graphite furnace atomic absorption spectrophotometer, and the levels of F detected with a fluoride ion-selective electrode. Enzyme-linked immunosorbent assay (ELISA) was used for assessing serum contents of PICP and PINP, and the sequence of COL1alpha1 gene was analyzed with ABI sequence analyzer.</p><p><b>RESULTS</b>The contents of PICP and PINP were similar between the two groups, which had also identical COL1alpha1 DNA sequences. After controlling for all the other factors, an inverse correlation was noted between PICP and serum F contents (r=-0.4, P<0.05) and between PINP and hair F content (r=-0.5, P<0.05), but a positive correlation was found between PINP and hair Se content (r=0.5, P<0.05).</p><p><b>CONCLUSION</b>Hair F and Se levels are the major factors affecting PINP synthesis, and increased hair F level is associated with elevated PINP content, whereas hair Se level is inversely correlated to PINP content. But in the patients with arsenic poisoning from Dazhuyuan town, the PICP, PINP contents and COL1alpha1 DNA sequence show no significant alterations, suggesting that hair Se increment abolishes the effect of hair F increment in inducing the decrease in PINP content.</p>

Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Arsenic Poisoning , Blood , Genetics , Case-Control Studies , China , Collagen Type I , Genetics , Peptide Fragments , Blood , Procollagen , Blood , Sequence Analysis, DNA
Chinese Journal of Endemiology ; (6): 416-418, 2008.
Article in Chinese | WPRIM | ID: wpr-642662


Objective To monitor the prevalence of Keshan disease in Huangling County, Shaanxi Previnee in 2007. Methods All local residents in surveinance area of Keshan disease in Diantou and Yaoping Township of Huangling County, were surveyed and given overall examinations by electrocardiography. The patients of latent and chronic Keshan disease were examined by X-ray. The selenium contents in blood and hair were monitored by fluorospectrophotometry in surveillance and non-surveillance areas. Results No new ease of acute or sub-acute patient was found. Latent and chronic patients detected accounted for 3.92% (35/893). Only 1 ease of new latent Keshan disease was newly found. Major feature of electrocardiographic abnormality was ST-T change. By X-ray, there were 27 eases with a normal heart, 20 eases with mild enlargement, 20 eases with medium enlargement, and 16 eases with marked enlargement. Serological selenium eoncentrations were (38.67±19.58), (48.55±22.36), (67.29±17.32)μg/L, respectively in patients, internal and external eontrols(F=16.291 ,P<0.01). Selenium contents of children's hair were (0.376±0.101), (0.372±0.085), (0.436±0.085)μg/L, respectively(F= 17.032, P<0.01). Conclusions Cases of diagnosed Keshan disease were decreased compared with the previous years. There were new eases of latent Keshan disease in Huangling County, Shaanxi Province.

Article in Chinese | WPRIM | ID: wpr-283129


<p><b>OBJECTIVE</b>To investigate the effect of organic gallium and gallium chloride on bone metabolism and their therapeutic effect against tretinoin-induced osteoporosis in rats.</p><p><b>METHODS</b>Rat models of osteoporosis was established with intragastric administration of tretinoin at the daily dose of 85 mg/kg for 15 days and randomized into control, organic gallium and gallium chloride groups. After administration of the corresponding treatments (none for the control group) for 4 weeks, the changes of the indices for osteoporosis were evaluated through biochemical and pathological approaches.</p><p><b>RESULTS</b>Tretinoin induced obvious changes in bone structure and contents of bone calcium and other elements, causing also significantly increased tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (AKP), which suggested the development of osteoporosis. Administration of organic gallium and gallium chloride treatments increased the bone density, bone cortex thickness and the percentage of bone trabecula, and Ga, Ca, P contents in the femur and teeth, but lowered the activity of TRAP and AKP, suggesting decreased bone conversion rate. Compared with gallium chloride, organic gallium required smaller dose with better safety to produce better therapeutic effect.</p><p><b>CONCLUSION</b>Organic gallium can be safe and effective for treatment of tretinoin-induced osteoporosis in rats.</p>

Animals , Female , Rats , Cell Line, Tumor , Femur , Metabolism , Pathology , Gallium , Chemistry , Pharmacology , Therapeutic Uses , Hemodynamics , Organometallic Compounds , Chemistry , Pharmacology , Therapeutic Uses , Osteoporosis , Drug Therapy , Metabolism , Rats, Sprague-Dawley , Tooth , Metabolism , Trace Elements , Metabolism , Tretinoin , Pharmacology