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Article in Chinese | WPRIM | ID: wpr-710684


Objective Through studying the extracorporeal membrane oxygenation (ECMO)alleviating kidney damage caused by unstable circulation function following brain deaths to improve the utilization rate of donation after brain death (DBD) for renal transplantation.Methods Randomly selected 60 DBD patients with unstable circulatory function were randomly divided into 3 groups according to the time of perfusion by ECMO.The first group used ECMO perfusion for 2 h,group 24 h and group 36 h.At the same time,20 cases of stable DBD were randomly selected as the control group.The incidence of primary graft failure,delayed graft function and acute rejection,renal function recovery time,urine volume on the first day after surgery,creatinine and urea nitrogen one year after surgery were compared between two groups.Results The incidence of delayed recovery of renal function in experimental groups 1,2,3 and control group was 10% (2/20),15% (3/20),5%(1/20) and 10% (2/20) respectively.The incidence of acute rejection in experimental groups 1,2,3 and control group was 30% (6/20),25% (5/20),35% (7/20) and 25% (5/20) respectively.There were no statistically significant differences between the experimental groups and the control group (P >0.05).The mean renal function recovery time in the experimental groups 1,2,3 and control group was (9.6 ± 2.0),(9.7 ± 2.7),(9.5 ± 1.9) and (7.3 ± 3.1) days respectively.The average urine volume in experimental groups 1,2,3 and control group was (3 405 ± 755),(3 300 ±600),(3 810±825),and (3 600 ± 80) mL respectively.In experimental groups 1,2,3 and control group,the average creatinine level was (92 ± 28),(92 ± 28),(101 ± 31),and (98 ± 30) μmol/L respectively.The average urea nitrogen in experimental groups 1,2,3 and control group was (4.2 ± 2.2),(5.6 ± 2.6),(5.2 ± 2.6) and (5.5 ± 2.5) mmol/L respectively.The recovery time of renal function in the experimental groups was longer than that in the control group (P<0.05),but there was no significant difference among the experimental groups (P>0.05).There was no statistically significant difference between the experimental groups and the control group in urine volume one day after surgery and kidney function one year after transplantation (P>0.05).Only recovery time after operation of the DBD with the unstable circulation function assisted by EMCO was slightly longer than that with stable circulation function,while the rest indexes showed no statistically significant difference.Conclusion DBD with unstable circulation function with ECMO auxiliary circulatory function can protect the renal function and increase the utilization rate of donor organs.

Article in Chinese | WPRIM | ID: wpr-923645


@#Objective To investigate the effect of Ski on the secretion of inflammatory cytokines from activated astrocytes. Methods Astrocytes were obtained from cerebral cortex of a three-day old Sprague-Dawley rat and cultured in vitro. They were divided into blank group, control group and siRNA group. The Ski gene was silenced in siRNA group. The expression of Ski was tested with Western blotting and immunofluorescence 48 hours later. Then the astrocytes were stimulated with lipopolysaccharide for 24 hours. The secretion of tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in activated astrocytes was detected with ELISA. Results The expression of Ski protein reduced in the siRNA group (P<0.001), as well as the secretion of TNF-α and IL-1β (P<0.001). Conclusion Ski may play a role in inflammatory response of astrocyte.