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1.
International Journal of Surgery ; (12): 329-334, 2020.
Article in Chinese | WPRIM | ID: wpr-863332

ABSTRACT

Objective:To study the inhibitory effect of CPT combined with 5-Fluorouracil (5-Fu), Oxaliplatin(L-OHP) on gastric carcinoma cells in vitro. Methods:MGC823 and SGC7901 gastric carcinoma cell lines were selected for culture and passage. The two cell lines were distributed into blank control group, CPT group, 5-Fu group, L-OHP group, 5-Fu+ CPT group and L-OHP+ CPT group. The blank control group was not given any drugs, CPT group was given CPT 1 000 μg/ml 2-fold dilution until 0.004 μg/ml; 5-Fu group was given 5-Fu 2.5×10 6 ng/ml 10-fold dilution until 2.5×10 -4 ng/ml; L-OHP group was given L-OHP 1 000 μg/ml 2-fold dilution up to 0.002 μg/ml; 5-Fu+ CPT group was given 5-Fu step concentration 2.5×10 6 ng/ml 10-fold dilution up to 2.5×10 -8 ng/ml, and combined with CPT 300 ng/ml; L-OHP+ CPT group was given a 2-fold dilution of L-OHP step concentration 1 000 μg/ml up to 0.24 μg/ml and combined with CPT 300 ng/ml. The inhibition effect of cells after drug action was detected. The 50% inhibitive concentration (IC 50) of medicines and the synergistic effect of the combined drugs were analyzed. The measurement data were expressed as Mean ± standard deviation ( Mean± SD), and the t-test was used for comparison between groups. Results:The inhibition rate of 5-Fu+ CPT group was significantly higher than that of 5-Fu group, the differences in MGC823 and SGC7901 cell lines were statistically significant ( P=0.002, 0.009). The inhibition rate of L-OHP+ CPT group was also significantly higher than that of L-OHP group, the differences in MGC823 and SGC7901 cell lines were statistically significant ( P=0.037, 0.040). Compared with 5-Fu+ L-OHP group, IC 50 value of 5-Fu+ CPT group and L-OHP+ CPT group decreased significantly, the differences in MGC823 and SGC7901 cell lines were statistically significant (all P<0.001). The survival fraction of 5-Fu and L-OHP combined with CPT on the two cell lines was lower than 70%, which was estimated by Weeb coefficient. Conclusions:The combination of 5-Fu, L-OHP and CPT can significantly improved the inhibitory effect on MGC823 and SGC7901 cell lines. 5-Fu, L-OHP and CPT had good synergistic effect.

2.
International Journal of Surgery ; (12): 326-328, 2020.
Article in Chinese | WPRIM | ID: wpr-863330

ABSTRACT

Objective:To explore the application of radiofrequency ablation in breast conserving surgery with breast carcinoma.Methods:With retrospective research methods, a total of 230 patients who were treated with radiofrequency ablation after breast conserving surgery in Beijing Friendship Hospital, Capital Medical University from January 2016 to September 2018 were selected, patients were all females, the median age was 56 yecers, ranged from 27 to 91 years, and the postoperative efficacy, patients′ satisfaction with their appearance and complications were outpatient follow-up.Results:Follow-up for 18 months showed that 2 (0.87%) patients had postoperative recurrence and metastasis, 217 (94.35%) patients were satisfied with the postoperative breast appearance, and 8 patients (3.48%) had skin burns and in- situ sclerosing. Conclusions:Radiofrequency ablation is used in breast conserving surgery, which is simple to operate and has a low incidence of complications. It can strengthen local tumor control and improve the cosmetic effect of breast after conserving surgery, worthy of clinical promotion.

3.
Article in Chinese | WPRIM | ID: wpr-863266

ABSTRACT

Objective To evaluate the efficacy of radiofrequency ablation in breast-conserving surgery followed neoadjuvant chemotherapy.Methods Retrospective analysis of 30 cases of breast cancer patients admitted to Beijing Friendship Hospital,Capital Medical University from April 2015 to September 2018.The average age was 54.3 years and the range was 28 to 70 years.For breast invasive ductal cancer,patients underwent breast-conserving surgery after neoadjuvant chemotherapy,and then received radiofrequency ablation.The clinical and pathological characteristics,postoperative complications,recurrence and metastasis,and cosmetic effects were observed.Results All 30 patients with breast-conserving surgery successfully completed radiofrequency ablation.The average outpatient follow-up was 22.5 months,ranging from 2 to 43 months,without local recurrence or metastasis;skin burns occurred in 2 cases (6.67%),and solid nodules formed in situ in 6 cases (20.00%);cosmetic satisfaction rate was 86.67% (26/30).Conclusions The radiofrequency ablation techniques applied in breast-conserving surgery have a high rate of technical success with relative low complication rates.And the radiofrequency ablation techniques may improve the cosmetic results after breast-conserving surgery and enhance local control.

4.
International Journal of Surgery ; (12): 28-31,封4, 2020.
Article in Chinese | WPRIM | ID: wpr-863265

ABSTRACT

Objective To explore the application effect of fine needle aspiration cytology and sentinel lymph nodes stain assisted by contrast-enhanced ultrasound in early breast cancer.Methods A patient with early breast cancer enrolled in Beijing Friendship Hospital,Capital Medical University received fine needle aspiration cytology assisted by contrast-enhanced ultrasonography and the sentinel lymph nodes were stained with blue dye before a standard sentinel lymph nodes biopsy traced with indocyanine green.The axillary status accessed by these two methods were compared.Results Three sentinel lymph nodes were found and aspirated assisted by contrast-enhanced ultrasonography.Seven sentinel lymph nodes were obtained in sentinel lymph nodes biopsy surgery.All of these sentinel lymph nodes were negative.The stained sentinel lymph nodes could be recognized and dissected in open lymph nodes biopsy surgery.Conclusions Fine needle aspiration cytology assisted by contrast-enhanced ultrasonography could be a substitute for open sentinel lymph nodes biopsy.More related researches should be carry out to further compare these two methods.

5.
Article in Chinese | WPRIM | ID: wpr-799273

ABSTRACT

Objective@#To evaluate the efficacy of radiofrequency ablation in breast-conserving surgery followed neoadjuvant chemotherapy.@*Methods@#Retrospective analysis of 30 cases of breast cancer patients admitted to Beijing Friendship Hospital, Capital Medical University from April 2015 to September 2018. The average age was 54.3 years and the range was 28 to 70 years. For breast invasive ductal cancer, patients underwent breast-conserving surgery after neoadjuvant chemotherapy, and then received radiofrequency ablation. The clinical and pathological characteristics, postoperative complications, recurrence and metastasis, and cosmetic effects were observed.@*Results@#All 30 patients with breast-conserving surgery successfully completed radiofrequency ablation. The average outpatient follow-up was 22.5 months, ranging from 2 to 43 months, without local recurrence or metastasis; skin burns occurred in 2 cases (6.67%), and solid nodules formed in situ in 6 cases (20.00%); cosmetic satisfaction rate was 86.67% (26/30).@*Conclusions@#The radiofrequency ablation techniques applied in breast-conserving surgery have a high rate of technical success with relative low complication rates. And the radiofrequency ablation techniques may improve the cosmetic results after breast-conserving surgery and enhance local control.

6.
International Journal of Surgery ; (12): 28-31,f4, 2020.
Article in Chinese | WPRIM | ID: wpr-799272

ABSTRACT

Objective@#To explore the application effect of fine needle aspiration cytology and sentinel lymph nodes stain assisted by contrast-enhanced ultrasound in early breast cancer.@*Methods@#A patient with early breast cancer enrolled in Beijing Friendship Hospital, Capital Medical University received fine needle aspiration cytology assisted by contrast-enhanced ultrasonography and the sentinel lymph nodes were stained with blue dye before a standard sentinel lymph nodes biopsy traced with indocyanine green. The axillary status accessed by these two methods were compared.@*Results@#Three sentinel lymph nodes were found and aspirated assisted by contrast-enhanced ultrasonography. Seven sentinel lymph nodes were obtained in sentinel lymph nodes biopsy surgery. All of these sentinel lymph nodes were negative. The stained sentinel lymph nodes could be recognized and dissected in open lymph nodes biopsy surgery.@*Conclusions@#Fine needle aspiration cytology assisted by contrast-enhanced ultrasonography could be a substitute for open sentinel lymph nodes biopsy. More related researches should be carry out to further compare these two methods.

7.
International Journal of Surgery ; (12): 392-396,封3, 2017.
Article in Chinese | WPRIM | ID: wpr-616791

ABSTRACT

Objective To analyze the clinical efficacy of the laparoscopic breast-conserving surgery combined with radiofrequency ablation to treat the early-stage breast cancer.Methods We collected 55 patients diagnosed early-stage breast cancer in retrospect,which started from January 2014 to December 2016.Twenty-seven of them were performed the laparoscopic breast-conserving surgery combined with radiofrequency ablation while others went through laparoscopic breast-conserving surgery without radiofrequency ablation.Meanwhile,we adopted the student t-test and the chi-square test to compare results of two groups.More specific,the main indexes of this study are including the post-operative local recurrence,the incidence of fat liquefaction or the incision-infection,operation time,post-operative hospital stay and the hospitalization expense.Results The laparoscopic breast-conserving surgery combined with radiofrequency ablation group had low local-recurrence than the laparoscopic breastconserving surgery group (0 and 7.69%).Additionally,there were no statistical differences between two groups in the incidence of fat liquefaction.However,The laparoscopic breast-conserving surgery combined with radiofrequency ablation group had more hospitalization expense than the laparoscopic breast-conserving surgery group [(4.1 ± 0.7) ten thousand yuan and (2.3 ± 0.6) ten thousand yuan,P < 0.05].Conclusions Although the laparoscopic breast-conserving surgery combined with radiofrequency ablation group remarkably increased the hospitalization expense because of the utility of the radiofrequency ablation related apparatus,it may provide the probability of shaving more residual tumor cell and may low down the recurrence,especially not rising up the incidence of the post-operative fat liquefaction.Therefore,this surgery method might be one of the potential developments in the minimal-invasive of early stage breast cancer.

8.
Chinese Medical Journal ; (24): 1947-1953, 2014.
Article in English | WPRIM | ID: wpr-248075

ABSTRACT

<p><b>BACKGROUND</b>Breast cancer is one of the most common malignant female diseases worldwide. It is a significant threat to every woman's health. Vascular endothelial growth inhibitor (VEGI) is known to be abundant in endothelial cells. According to previous literature, overexpression of VEGI has been shown to inhibit tumor neovascularisation and progression in cellular and animal models, but there has been limited research on the significance of VEGI in the breast cancer.</p><p><b>METHODS</b>In our study, cell lines MDA-MB-231 were first constructed in which VEGI mediated by lentivirus over-expressed. The effects of VEGI over-expression on MDA-MB-231 cells were investigated both in vitro and in vivo. The expression of VEGI in the MDA-MB-231 cells after infection of lentivirus was analyzed using real-time PCR and Western blotting. The effect of the biological characteristics of MDA-MB-231 cells was assessed by growth, invasion, adhesion, and migration assay with subcutaneous tumor-bearing nude mice models. Then the growth curves of the subcutaneous tumors were studied. Expressions of VEGI, CD31 and CD34 in the tumors were analyzed by immunohistochemistry and apoptosis was detected by flow cytometry and immunohistochemistry.</p><p><b>RESULTS</b>Infection of MDA-MB-231 cells within the lentivirus resulted in approximately a 1 000-fold increase in the expression of VEGI. As can be seen in the invasion, adhesion and migration assay, the over-expression of VEGI can inhibit the ability of MDA-MB-231 cells during migration, adhesion and invasion. The volume of the subcutaneous tumor in the over-expression group was distinctly and significantly less than that of the control groups. Immunohistochemistry analysis of the tumor biopsies clearly showed the expression of VEGI in the over-expression group increased while CD31 and CD34 decreased significantly. In vitro and in vivo, the early apoptosis rate and the apoptosis index were increased within the VEGI over-expression group as compared with the control group.</p><p><b>CONCLUSIONS</b>Taken together, recombinant lentivirus that were successfully constructed, demonstrated up-regulated VEGI gene expression in breast cancer cells. Lentivirus-mediated over-expression of VEGI weakened the ability of the breast cancer cell migration, adhesion and invasion. Over-expression of VEGI diminished the tumorigenic capacity of breast cancer cells in vivo. Up-regulation of VEGI gene expression however inhibited breast cancer MDA-MB-231 cell in the early apoptosis.</p>


Subject(s)
Apoptosis , Genetics , Physiology , Breast Neoplasms , Genetics , Metabolism , Pathology , Cell Line, Tumor , Cell Proliferation , Genetics , Physiology , Female , Gene Expression Regulation, Neoplastic , Genetics , Physiology , Genetic Vectors , Genetics , Humans , Lentivirus , Genetics , Vascular Endothelial Growth Factors , Genetics , Metabolism
9.
Article in Chinese | WPRIM | ID: wpr-418106

ABSTRACT

Objective To evaluate the diagnostic value of ultrasonography (USG) and contrast-enhanced computer tomography (CT) for thyroid carcinoma.MethodsThe results of the review of the preoperative CT and those of the original US reports were compared with the histopathologic results in 73 cases of thyroid carcinoma who underwent radical operation and central compartment dissection from November 2009 to November 2011.ResultsThe final diagnostic rate of thyroid carcinoma with USG was 69.9%,CT of that was 80.8%.There were no significant difference between them (P =0.077).The diagnostic rate of metastatic lymph node in the central compartment with USG was 61.6%,positive predictive value was 64.0%,negative predictive value was 60.4%.The diagnostic rate of metastatic lymph node in the central compartment with CT was 67.1%,positive predictive value was 61.7%,negative predictive value was 76.9%.There were not significant difference between them(P =0.848,P =0.152,P =0.489).Conclusion There was no significant difference between USG and CT in The final diagnostic rate of thyroid carcinoma and its lymph node metastasis in the central compartment.

10.
Article in Chinese | WPRIM | ID: wpr-417399

ABSTRACT

Objective To analyze clinical characteristics of invasive Luminal subtype breast cancer.Methods The data of 162 invasive Luminal subtype breast cancer patients receiving operation in Cancer Hospital of Chinese Academy of Medical Science from January 1 st to September 30th in 2002,were collected and the clinical characteristics,recurrences,metastasis and survivals were retrospectively analyzed.Results The median time of follow-up was 92 months,ranging from 4 to 98 months.41 cases (25.3%,41/162) presented local recurrence or metastasis including 32 cases with metastasis ( 19.8%,32/162),2 cases with local recurrences (1.2%,2/162) and 7 cases with both local recurrence and metastasis (4.3%,7/162) ;Disease-free survival (DFS) and the 5-year DFS were 73.1% and 79.6%,respectively.27 patients ( 16.7%,27/162) died of breast cancer,the overall survival (OS) and 5-year OS were 82.5% and 85.3%,respectively.According to Kaplen-Meier survival analysis,tumor size,lymph node status and clinical stage were correlated to overall survival time ( P < 0.05 ) ; and rumor size,lymph node starus,grade,clinical stage and PR status were correlated to disease-free survival time ( P < 0.05 ).By multivariate analysis,TNM stage,PR and PCNA were independent prognostic factors correlated to overall survival time (OR=0.633,95% CI:0.411 -0.976,P<0.05; OR =0.823,95% CI:1.012-3.283,P < 0.05) ; TNM stage and PR was independent prognostic factors correlated to disease-free survival time (OR =3.273,95% CI:1.719 - 6.232,P < 0.01 ; OR =0.599,95% CI:0.423 - 0.850,P < 0.01 ).Conclusions In invasive Luminal subtype breast cancers,PR is correlated to fine prognosis,and PCNA is correlated to overall survival time.

11.
Article in Chinese | WPRIM | ID: wpr-414715

ABSTRACT

Objective To evaluate the clinical value and experience of endoscopic thyroidectomy.Methods Thyroidectomy with endoscopic instruments through chest-wall approach was done for 52 patients who had thyroid adenoma nodular goiter or thyroid cancer.We observed the results and complications of these operations.Results All 52 cases of operation were successful with no conversion.There was no nerve damage or postoperative hemorrhage.Average operation duration was (100 ± 22) min.The mean time of hospitalization after operation was (3 ± 0.5) d.Conclusion With the advantages of minimal and hidden incision,sooner recovery and satisfactory cosmetic effect,the endoscopic thyroidectomy will prevail in selected cases in the future.

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