ABSTRACT
ABSTRACT In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross-amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa.
Subject(s)
Animals , Perciformes/genetics , Polymerase Chain Reaction/methods , Phylogeny , Genetic Variation , Perciformes/classification , Genetic Markers , Sequence Analysis, DNA , Genetics, PopulationABSTRACT
Objective To assess the value of three clinical diagnostic criteria systems,the APACHE-Ⅱ,Balthazar score and Extrapancreatic inflammation on Abdominal Computed tomography(EPIC) in predicting outcome of patients with acute pancreatitis.Methods One hundred and seventy-eight patients with acute pancreatitis who underwent an abdominal CT scan within 24 h after admission were studied prospectively in the study.To assess the value of predicting severe AP and complications,the APACHE-Ⅱ score,the Balthazar score,EPIC score were calculated and evaluated by calculating receiver operator characteristic(ROC)curves,the sensitivity,the specificity,the Youden Index and the area under the ROC curve.Results 46 of 178 patients developed severe disease.The mean score of the three criteria system was significantly higher in the group of severe AP than the mild AP one(P