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1.
Article | IMSEAR | ID: sea-242111

ABSTRACT

Background: Implementing both Internal Quality Control (IQC) and External Quality Assessment Schemes (EQAS) is necessary to ensure the quality of the reports released by the Clinical Laboratories. EQAS helps us assess our laboratory's performance by comparing it to that of peer laboratories, providing insights into our quality standards and identifying areas for improvement. The objectives were to interpret the results of the Biochemical parameters of the external quality assessment scheme and to analyse the performance of the laboratory by using indicators of the external quality assessment scheme. Methods: This retrospective observational study was conducted in a Clinical Biochemistry Laboratory at a Tertiary Care Centre with clearance from the Institutional Ethics Committee. Two analysers were involved: the VITROS 250 and the VITROS ECI hormone analyser. In 2022, 31 biochemistry parameters were registered under the EQAS program by Randox (RIQAS). Results for each parameter were compared to the mean to generate performance statistics, including SDI, Target Score, and %Deviation. The analysis, covering RIQAS reports from 2022 and 2023, utilized Windows 10, MS Excel, and SPSS version 20 to calculate yearly averages and assess performance based on monthly target scores and deviations. Results: Iron (target score:48.82) and Lactate (SDI:3.0) were outliers in the year 2022, whereas in 2023, Aspartate transaminase (AST) (SDI:2.57) and Lactate dehydrogenase (LDH) (SDI:2.45) were the outliers. Conclusion: Two years of EQAS experience in our laboratory demonstrated satisfactory results, reinforcing confidence in the quality of patient reports. This study also helped identify potential risks and develop strategies to address them.

2.
J Biosci ; 2024 May; 49: 1-19
Article | IMSEAR | ID: sea-237974

ABSTRACT

Metabolism is the key cellular process of plant physiology. Understanding metabolism and its dynamical behavior under different conditions may help plant biotechnologists to design new cultivars with desired goals. Computational systems biochemistry and incorporation of different omics data unravelled active metabolism and its variations in plants. In this review, we mainly focus on the basics of flux balance analysis (FBA), elementary flux mode analysis (EFMA), and some advanced computational tools. We describe some important results that were obtained using these tools. Limitations and challenges are also discussed.

3.
Article | IMSEAR | ID: sea-240046

ABSTRACT

Background: Biochemistry is a crucial science in medical education, traditionally taught through didactic lectures and practical sessions. However, a shift toward a student-centric approach is promoting lifelong self-directed learning and motivation. Case-based learning (CBL) and interactive didactic lectures (IDL) are two methodologies, with CBL fostering critical thinking, problem-solving, and decision-making skills, while interactive lectures may not be as effective. Aims and Objectives: The objectives of the research were to evaluate the effectiveness of CBL in comparison with IDL in teaching clinical biochemistry to 1st-year MBBS students and to analyze the perceptions of both students and faculty members regarding CBL. Materials and Methods: The study used a pre-validated questionnaire and a pre- validated perception questionnaire to collect data from students and faculty members. The students were divided into two groups, with group A having odd roll numbers and group B having even roll numbers. The study was divided into two sessions. In session 1, both groups were assessed on their baseline knowledge through a pre-test on Topic 1, with group A receiving CBL and group B receiving an IDL. Post-tests were taken to evaluate understanding and retention of the material. In session 2, both groups underwent another pre-test on topic 2, with group A receiving an IDL and group B receiving CBL. Post-tests were also conducted to assess understanding and retention. Results: The results showed that both groups had similar pre-test performance, but the CBL group showed a significant improvement in post-test performance compared to the IDL group. In addition, both students and faculty members had a positive perception of CBL, with the majority agreeing that it improved teacher–student communication, facilitated active participation, enhanced understanding of key concepts, and promoted knowledge application. Conclusion: The study suggests that CBL is an effective teaching method for medical education, aligning with modern educational approaches that emphasize student-centered learning. The positive feedback and improved academic performance support the integration of CBL into medical curricula, promoting meaningful learning and preparing students for lifelong self- directed learning.

4.
Article | IMSEAR | ID: sea-241105

ABSTRACT

Canine pyometra is a common reproductive disorder of intact diestrual bitches. The disease is associated with inflammation and infection of the uterus leading to generalized illness. The varied reactions of the host to infection, inflammation, or trauma are collectively known as the acute-phase response (APR) and encompass a wide range of pathophysiological responses. One of these systemic responses to disease is an increase in the production by the liver of a number of serum proteins, which are known collectively as the acute-phase proteins (APP). The most important APPs in dogs are C-reactive protein (CRP) and serum amyloid A (SAA). When diagnosed with pyometra, the preferred and most effective treatment is ovariohysterectomy, a procedure that in itself causes systemic inflammation due to the tissue trauma caused by surgery. The purpose of this study was to identify the effect pyometra and ovariohysterectomy have on acute phase proteins and routinely measured biochemistry variables in serum of affected bitches. We achieved this by evaluating concentrations of CRP and SAA, as well as of some biochemistry parameters in serum before and after ovariohysterectomy in bitches with pyometra. Blood samples were collected from the cephalic vein immediately prior to and 24h, 72h and 168 hours after initiation of surgery. The levels of CRP, SAA and some biochemistry parameters - total protein (TP), albumin (ALB), globulin (GLOB), as well as activity of some enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AKLP) and lactate dehydrogenase (LDH) were determined Aims: The aim of this study was to investigate the impact of pyometra and its surgical treatment, ovariohysterectomy, on acute phase proteins, specifically C-reactive protein (CRP) and serum amyloid A (SAA), as well as on routine serum biochemistry variables in affected bitches. By evaluating the concentrations of CRP and SAA, alongside key biochemistry parameters before and after surgery, this research seeks to elucidate the physiological and biochemical responses to both the disease and its treatment, providing insight into the systemic effects of pyometra and the surgical stress response in dogs. Study Design: The study design involved a cohort of twenty-seven female dogs, divided into two groups: seventeen clinically healthy intact female dogs serving as the control group, which were examined for routine health care or vaccinations, and ten female dogs diagnosed with pyometra at the Faculty of Veterinary Medicine, University of Sarajevo. The diagnosis of pyometra was made based on case history, clinical signs, ultrasonography, and confirmed by gross examination of the pus-filled uterus during and after ovariohysterectomy. The surgical procedure was performed using a standard mid-line approach under general inhalation anesthesia, with no post-operative complications reported. Place and Duration of Study: Faculty of Veterinary Medicine, University of Sarajevo, between June 2012 and September 2012. Methodology: Blood samples were collected from the distal cephalic vein into serum separating tubes (Vacutainer SST; Becton Dickinson, USA) for CRP and SAA measurement and serum biochemical evaluation just before the ovariohysterectomy (base values) as well as after 24 hours (day one), 72 hours (day three) and 168 hours (day seven). Blood samples from control dogs were collected only once. Serum samples were prepared by centrifugation (1.500 × g for 10 min) and stored in plain micro tubes (Eppendorf, Germany) at ?20°C prior to analysis. Serum CRP concentrations in the dogs were measured with ELISA microplate reader (Anthos 2001 ELISA-reader, Anthos Mikrosysteme, Krefeld, Germany) using a commercial canine CRP enzyme-linked immunosorbent assay (ELISA) kit (Tridelta PhaseTM Range CRP Assay, Tridelta Development Limited, County Kildare, Ireland). For SAA, the analyses were performed using also a commercially available ELISA kit (Tridelta PhaseTM Range SAA Assay, Tridelta Development Limited, County Kildare, Ireland). Total protein (TP), albumin (ALB), globulin (GLOB) concentrations and alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALKP) and lactate dehydrogenase (LDH) activities were determined by using standard procedures and slides by Catalyst Dx Chemistry Analyzer, Idexx. Statistical analyses were performed using the SPSS software package (for Windows, Version 11.5, SPSS Inc, USA). All data were shown as mean ± standard deviation. The results were analyzed using the independent t-test. A P-value less than 0.05 were considered statistically significant. Results: Our results indicated significantly higher concentrations of CRP and SAA in dogs with pyometra throughout the study period compared to the control group, with CRP concentrations rising up to 15-fold and SAA up to 10-fold. Notably, these increases in CRP and SAA concentrations were mostly observed before surgery, with only limited additional increases post-surgery. This suggests that while ovariohysterectomy induces a proinflammatory response, it does not significantly escalate the levels of these proteins beyond the initial disease impact. The variability observed in CRP and SAA levels points to potential interindividual differences, impacting their diagnostic value. However, on the last postoperative day, the significantly elevated levels of both proteins in dogs with pyometra suggest a persistent, though less severe, inflammatory process, with a subsequent decline as homeostasis was gradually restored. Conclusion: The role of the acute phase response is to limit inflammation, remove the damaging factor and restore homeostasis. The systemic effects of pyometra are reflected by acute phase proteins and several biochemistry parameters.

5.
Article | IMSEAR | ID: sea-240759

ABSTRACT

Evidence-based medicine is basically a positivistic, biomedical perspective. Its focus is on offering clinicians the best available evidence about the most adequate treatment for their patients. In the Era of Evidence based Medicine, the Diagnosis & Treatment protocol is largely dependent on the Diagnostic Tests of which a lions part is Biochemical Tests. Due to Automation many companies are coming up with new machines everyday. There lies the importance of Validity of an instrument. Authors planned this study in order to provide the Quality assured biochemical testing by validating the new autoanalyzer by comparing the results with an existing EQAS proven autoanalyzer. Comparative analysis chart of test results obtained by both the instruments were drawn, Comparative analysis was done, Statistical Linear Regression plot & Bland Altman plot were also drawn. 22 Unacceptable values & 18 Acceptable values out of 40 run in BIORAD Control Target Values of 10 Tested Parameters in case of Tested Analyzer.While all 40 are Acceptable values out of 40 run in BIORAD Control Target Values in case of Existing Analyzer obtained. ALT, T. Bilirubin, AST, Creatinine, Triglycerides Showing Negative bias. Glucose Showing Positive bias. Total Protein, Albumin not showing good correlation. ALP Showing High Constant error.

6.
Article | IMSEAR | ID: sea-233854

ABSTRACT

Background: It is an undeniable fact that assessment drives learning. It is documented that traditional practical examination has several drawbacks. Further, the subjectivity also affects the correlation negatively between marks awarded by different examiners and therefore on the performance of an equivalent candidate. The objective of objective structured practical examination (OSPE) is to reduce the chances of examiner bias and assess the students� skills. Aim was to introduce objective structured practical examination (OSPE) as a method of assessment of practical skills in Biochemistry and to determine the perception of students� regarding OSPE. Methods: It is a qualitative, observational study conducted at Department of Biochemistry, Assam Medical College, Assam, India. The first Professional MBBS students were evaluated by using OSPE to assess their practical perspective in Biochemistry. A pre-validated questionnaire-based on a 5-point Likert scale and descriptive type on various components of the OSPE was administered to get the feedback. Statistical analysis used: Microsoft Excel and GraphPad Prism 8 software were applied. Results: Most of the students favoured OSPE as an assessment tool. About 82% of students agreed that OSPE helps to score better; 62.3% stated that OSPE eliminates bias in exams; 91.6% supported OSPE to be incorporated as a formative assessment. Conclusions: The study favours OSPE as a reliable assessment tool for practical examination in biochemistry. OSPE is more objective and eliminates examiner bias and helps to evaluate all three learning domains - cognitive, psychomotor and affective domains.

7.
Article | IMSEAR | ID: sea-240140

ABSTRACT

Background: Development of high sensitivity CRP (Hs-CRP) assays has improved the role of this acute phase reactant in predicting the first cardiovascular events. Hs-CRP levels partially reflect the extent of myocardial necrosis and can be used to predict inhospital and long-term outcome in patients with acute myocardial infarction. Aims and Objectives: The objectives of the study are to study and correlate increased level of Hs-CRP with various traditional risk factors such as age, sex, lifestyle, and comorbidities and to establish increased level of Hs-CRP as an independent marker for cardiovascular events. Materials and Methods: A 100 patients’ prospective cross-sectional study was conducted at tertiary care center with traditional risk factors and existing cardiovascular diseases. Measurements of Hs-CRP levels were done with immunoturbidimetric methods with statistical correlation. The statistical analysis was done using IBM SPSS version 26 software. Results: In total 100 patients, 67 patients had Hs-CRP value more than 3 mg/L where-as 33 patients had Hs-CRP value <3 mg/L, 32% of patients belonged to age group 51–60 years and majority 78% were male. Among all the individual life style parameters and traditional risk factor which were considered, five parameters, namely, sedentary life style, smoking, pre-existing hypertension, diabetes mellitus, and electrocardiogram changes were proved statistically significant and correlation between elevated high sensitivity C reactive protein and cardiovascular disease was proved statistically significant. Conclusion: Hs-CRP is independent biomarker for cardiovascular disease and positive correlation was established between elevated level of Hs-CRP and major traditional risk factors such as sedentary life style, smoking, hypertension, and diabetes mellitus.

8.
Article in Chinese | WPRIM | ID: wpr-1024347

ABSTRACT

Objective To explore the changes of metabonomics in blood of mice after high-voltage electric shock,then screen out the significantly changed differential metabolites and metabolic pathways.Methods The head of C57BL/6J mice was subjected to high-voltage electric shock(electric shock group)or exposed to acoustic and optical stimulation of high-voltage electric(control group),then the whole blood from mice were collected to separate serum.The dual platform combined metabonomic analysis based on gas chromatography-mass spectrometer(GC-MS)and liquid chromatography-mass spectrometer(LC-MS)was performed and orthogonal partial least squares discriminant analysis(OPLS-DA)was used to screen the differential metabolites and related metabolic pathways.Results A total of 415 differential metabolites were screened out in metabonomics in blood of mice after high-voltage electric shock,including 187 up-regulated and 228 down-regulated metabolites.These differentially metabolites were significantly enriched in metabolic pathways including central carbon metabolism in cancer,glucagon signaling pathway,etc.Conclusion By establishing the model of high-voltage electrical injury on experimental mice,this study reveals the significant change of metabolite content and metabolic pathway in blood by high-voltage electrical injury.Which provides a basis for the damage of blood metabolic activity by high-voltage electrical injury,and suggests the potential harm of high-voltage electrical injury to blood metabolic activity in the whole body.

9.
Article in Chinese | WPRIM | ID: wpr-1007269

ABSTRACT

The discrimination of the cause of death is an important part in forensic medicine. With the advantages of simple operation, high test efficiency, and easy sensitivity to the death mechanism of the deceased, postmortem biochemistry analysis has become a significant auxiliary analysis method for discrimination of the cause of death. In this paper, we discuss the development status, advantages and current problems of postmortem biochemical analysis, and then describe the application prospect of postmortem biochemistry in the discrimination of the cause of death by posing feasible solutions, so that to provide an idea for this research.

10.
Braz. j. biol ; 842024.
Article in English | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469308

ABSTRACT

Abstract This study was aimed to investigate Carbofuran (CF)-induced pathological changes in cattle egret. Two hundred cattle egrets were reared and equally divided into four groups and given different CF concentrations (0.03 mg/L, 0.02 mg/L, 0.01 mg/L and 0 mg/L (control group)). Hematology, serum biochemistry, histopathology, and immunological markers were studied. Our results confirm that CF induces anemic conditions, leukocytosis, elevated liver enzymatic activity, and alterations in renal biomarkers. Moreover, specific microscopic lesions such as multifocal necrosis, pyknotic nuclei, hemorrhages, congestion, and inflammatory cell proliferation were observed in the liver, kidney, spleen, and thymus. These findings suggest that CF can induce harmful effects, so the application of this pesticide in the field must be strictly monitored to mitigate the possibility of exposure to non-target species.


Resumo Este estudo teve como objetivo investigar as alterações patológicas induzidas por carbofurano (CF) em garças-vaqueiras. Duzentas dessas garças foram criadas e divididas igualmente em quatro grupos e receberam diferentes concentrações de CF: 0,03 mg/L; 0,02 mg/L; 0,01 mg/L; e 0 mg/L (grupo controle). Foram realizadas análises de hematologia, bioquímica sérica, histopatologia e marcadores imunológicos. Nossos resultados confirmaram que CF induz condições anêmicas, leucocitose, atividade enzimática hepática elevada e alterações nos biomarcadores renais. Além disso, lesões microscópicas específicas, como necrose multifocal, núcleos picnóticos, hemorragias, congestão e proliferação de células inflamatórias, foram observadas no fígado, rim, baço e timo. Esses achados sugerem que o CF pode causar efeitos nocivos, portanto a aplicação desse agrotóxico no campo deve ser rigorosamente monitorada para mitigar a possibilidade de exposição a espécies não alvo.

11.
Braz. j. biol ; 842024.
Article in English | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469322

ABSTRACT

Abstract Newcastle disease (ND) is an infectious, highly contagious and lethal disease of avian species. It is considered that ducks are natural reservoir or carrier for Newcastle disease virus (NDV) and are resistant against different strains of NDV. Current study was designed to evaluate the pathogenesis of Newcastle disease in domestic ducks through histopathology, immunohistochemistry (IHC) and serum biochemical changes. For this purpose, eighty ducks were reared for 42 days and divided in two groups A and B. Ducks in group A were challenged with (NDV) at rate of 0.1 ml of ELD50 (virus titer 107.32/100µl) on second week of age, whereas Group B was control negative. Splenomegaly, atrophy of thymus and necrotic lesion in kidney were observed on 9th day of post infection. Hepatic degeneration and mononuclear cell infiltration were noticed in proventriculus and intestine in challenged ducks. Viral antigen detected in lungs, intestine, proventriculus and lymphoid organs of infected ducks through IHC. Albumin and total protein values were significantly low in infected groups A as compared to control group B. ALT, AST, and ALP values were significantly high in infected group A. On 5th and 7th day of post infection oropharyngeal swabs were negative for NDV and cloacal swabs were positive for NDV through Reverse transcriptase polymerase chain reaction. It is concluded that ducks are susceptible to NDV and virulent strain of NDV caused disease in ducks.


Resumo A doença de Newcastle (DN) é uma doença infecciosa, altamente contagiosa e letal de espécies aviárias. Considera-se que os patos são reservatórios ou portadores naturais do vírus da doença de Newcastle (VDN) e são resistentes a diferentes cepas de VDN. O presente estudo foi desenvolvido para avaliar a patogênese da DN em patos domésticos por meio de histopatologia, imuno-histoquímica (IHQ) e alterações bioquímicas séricas. Para este propósito, 80 patos foram criados por 42 dias e divididos em dois grupos A e B. Os patos do grupo A foram submetidos ao VDN a uma taxa de 0,1 ml de ELD50 (título viral de 107,32 / 100 µl) na segunda semana de idade, enquanto o Grupo B foi controle negativo. Esplenomegalia, atrofia do timo e lesão necrótica no rim foram observadas no 9º dia pós-infecção. Degeneração hepática e infiltração de células mononucleares foram observadas no proventrículo e intestino em patos infectados. Antígeno viral foi detectado em pulmões, intestino, proventrículo e órgãos linfoides de patos infectados por IHQ. Os valores de albumina e proteína total foram significativamente baixos no grupo A infectado em comparação com o grupo B. Os valores de ALT, AST e ALP foram significativamente altos no grupo A. No 5º e no 7º dia após a infecção, os esfregaços orofaríngeos foram negativos para VDN, enquanto os esfregaços cloacais foram positivos para VDN por meio da reação em cadeia da polimerase via transcriptase reversa. Conclui-se que os patos são suscetíveis ao VDN e à cepa virulenta de VDN que causou doenças em patos.

12.
Acta bioquím. clín. latinoam ; Acta bioquím. clín. latinoam;58(2): 155-161, 2024. graf
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1568710

ABSTRACT

Resumen La Práctica Profesional es el último eslabón en la cadena de formación de los estudiantes de Bioquímica. El objetivo del presente trabajo fue realizar una reseña de las innovaciones incorporadas en la asignatura desde la creación de la carrera de Bioquímica en la Universidad Nacional de Misiones en el año 1974. El objetivo del presente trabajo fue realizar una reseña de las innovaciones incorporadas en la asignatura desde la creación de la carrera de Bioquímica en la Universidad Nacional de Misiones en el año 1974. Desde modalidad de cursado de las actividades prácticas, incorporación de actividades teóricas, interacción con el equipo de salud y con la comunidad, hasta diferentes instancias de evaluación. Se considera que la formación de los alumnos en esta etapa final de la carrera debe ser amplia, de manera que estos puedan elegir dentro de la gran oferta de posibilidades laborales que posee esta profesión, aquella con la cual se sientan identificados. Esto es posible si se ofrece a los estudiantes en las Prácticas Profesionales una visión integral de la profesión bioquímica.


Abstract Professional Practice is the final link in the training chain of the course of studies for Biochemistry students. The objective of this work was to provide an overview of the innovations incorporated into the subject since the creation of the Biochemistry degree at the Universidad Nacional de Misiones in 1974. These include the mode of practical activities, the incorporation of theoretical activities, the interaction with the health care team and the community, as well as different assessment instances. It is considered that the training of students in this final stage of their degree should be comprehensive, so that they can choose from the wide range of job possibilities offered by this profession, selecting the one with which they feel identified. This is possible if students are offered a comprehensive vision of the biochemical profession in Professional Practice.


Resumo A Prática Profissional é o último elo da cadeia de formação dos alunos de Bioquímica. O objetivo deste trabalho foi fazer uma resenha das inovações incorporadas na disciplina desde a criação do curso de Bioquímica na Universidade Nacional de Misiones em 1974. Da modalidade de curso das atividades práticas, incorporação de atividades teóricas, interação com a equipe de saúde e com a comunidade, às diferentes instâncias de avaliação. Considera-se que a formação dos estudantes nesta fase final do curso deve ser ampla, para que eles possam escolher dentro do vasto leque de possibilidades de trabalho que esta profissão tem, aquela com a qual se sintam identificados. Isso é possível se oferecer aos estudantes nas Práticas Profissionais uma visão abrangente da profissão bioquímica.

13.
Arq. bras. neurocir ; 43(4): 269-277, 2024.
Article in English | LILACS-Express | LILACS | ID: biblio-1581216

ABSTRACT

Objective This study investigated the relationship between radiological morphological findings and hormone levels in patients with empty sella. Methods Patients (32 female, 3 male) with empty sella detected on radiological images were grouped as "partial empty sella (PBS) group" (subarachnoid space volume < 70%) and "total empty sella (TBS) group" (subarachnoid space volume > 70%). Age, gender, sella volume, pituitary gland thickness, the ratio of subarachnoid space height to pituitary gland height, and diaphragm sellae diameter were measured on radiological images. All patients' blood count results, biochemistry results, and serum hormone values were recorded. Results Age, complete blood count, serum biochemistry parameters, and hormone levels were not different between PBS and TBS groups. Correlation analysis revealed a negative correlation between gender and subarachnoid space invasion volume, between gender and sagittal width of the sella turcica (ST), between cortisol level and ST axial diameter, between progesterone level and ST axial diameter, between testosterone level and ST axial diameter, between adrenocorticotropic hormone level and ST axial diameter, between TSH level and subarachnoid space invasion rate, between free T4 level and diaphragm sellae sagittal width, and between free T4 level and ST sagittal width. Conclusion This study showed that some serum hormone values may decrease when the sella tursica dimensions or the volume of subarachnoid space invading the sella tursica increase in patients with empty sella, and therefore, periodic follow-up of hormone levels and radiological images of these patients would be appropriate.


Objetivo Este estudo investigou a relação entre achados morfológicos radiológicos e níveis hormonais em pacientes com sela vazia. Métodos Pacientes (32 mulheres 3 homens) com sela vazia detectada em imagens radiológicas foram agrupados como "grupo sela parcialmente vazia (PBS)" (volume do espaço subaracnóideo < 70%) e "grupo sela totalmente vazia (TBS)" (volume do espaço subaracnóideo > 70%). Idade sexo volume da sela espessura da glândula pituitária a razão entre a altura do espaço subaracnóideo e a altura da glândula pituitária e o diâmetro do diafragma da sela foram medidos em imagens radiológicas. Os resultados do hemograma bioquímica e valores hormonais séricos de todos os pacientes foram registrados. Resultados Idade hemograma completo parâmetros bioquímicos séricos e níveis hormonais não foram diferentes entre os grupos PBS e TBS. A análise de correlação revelou uma correlação negativa entre gênero e volume de invasão do espaço subaracnóideo entre gênero e largura sagital da sela túrcica (ST) entre nível de cortisol e diâmetro axial do ST entre nível de progesterona e diâmetro axial do ST entre nível de testosterona e diâmetro axial do ST entre nível de hormônio adrenocorticotrófico e diâmetro axial do ST entre nível de TSH e taxa de invasão do espaço subaracnóideo entre nível de T4 livre e largura sagital do diafragma da sela e entre nível de T4 livre e largura sagital do ST. Conclusão Este estudo mostrou que alguns valores hormonais séricos podem diminuir quando as dimensões da sela túrsica ou o volume do espaço subaracnóideo que invade a sela túrsica aumentam em pacientes com sela vazia e portanto o acompanhamento periódico dos níveis hormonais e imagens radiológicas desses pacientes seria apropriado.

14.
Braz. j. biol ; 84: e255055, 2024. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1355865

ABSTRACT

Abstract This study was aimed to investigate Carbofuran (CF)-induced pathological changes in cattle egret. Two hundred cattle egrets were reared and equally divided into four groups and given different CF concentrations (0.03 mg/L, 0.02 mg/L, 0.01 mg/L and 0 mg/L (control group)). Hematology, serum biochemistry, histopathology, and immunological markers were studied. Our results confirm that CF induces anemic conditions, leukocytosis, elevated liver enzymatic activity, and alterations in renal biomarkers. Moreover, specific microscopic lesions such as multifocal necrosis, pyknotic nuclei, hemorrhages, congestion, and inflammatory cell proliferation were observed in the liver, kidney, spleen, and thymus. These findings suggest that CF can induce harmful effects, so the application of this pesticide in the field must be strictly monitored to mitigate the possibility of exposure to non-target species.


Resumo Este estudo teve como objetivo investigar as alterações patológicas induzidas por carbofurano (CF) em garças-vaqueiras. Duzentas dessas garças foram criadas e divididas igualmente em quatro grupos e receberam diferentes concentrações de CF: 0,03 mg/L; 0,02 mg/L; 0,01 mg/L; e 0 mg/L (grupo controle). Foram realizadas análises de hematologia, bioquímica sérica, histopatologia e marcadores imunológicos. Nossos resultados confirmaram que CF induz condições anêmicas, leucocitose, atividade enzimática hepática elevada e alterações nos biomarcadores renais. Além disso, lesões microscópicas específicas, como necrose multifocal, núcleos picnóticos, hemorragias, congestão e proliferação de células inflamatórias, foram observadas no fígado, rim, baço e timo. Esses achados sugerem que o CF pode causar efeitos nocivos, portanto a aplicação desse agrotóxico no campo deve ser rigorosamente monitorada para mitigar a possibilidade de exposição a espécies não alvo.


Subject(s)
Animals , Carbofuran/toxicity , Birds , Cattle
15.
Braz. j. biol ; 84: e250607, 2024. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1355881

ABSTRACT

Abstract Newcastle disease (ND) is an infectious, highly contagious and lethal disease of avian species. It is considered that ducks are natural reservoir or carrier for Newcastle disease virus (NDV) and are resistant against different strains of NDV. Current study was designed to evaluate the pathogenesis of Newcastle disease in domestic ducks through histopathology, immunohistochemistry (IHC) and serum biochemical changes. For this purpose, eighty ducks were reared for 42 days and divided in two groups A and B. Ducks in group A were challenged with (NDV) at rate of 0.1 ml of ELD50 (virus titer 107.32/100µl) on second week of age, whereas Group B was control negative. Splenomegaly, atrophy of thymus and necrotic lesion in kidney were observed on 9th day of post infection. Hepatic degeneration and mononuclear cell infiltration were noticed in proventriculus and intestine in challenged ducks. Viral antigen detected in lungs, intestine, proventriculus and lymphoid organs of infected ducks through IHC. Albumin and total protein values were significantly low in infected groups A as compared to control group B. ALT, AST, and ALP values were significantly high in infected group A. On 5th and 7th day of post infection oropharyngeal swabs were negative for NDV and cloacal swabs were positive for NDV through Reverse transcriptase polymerase chain reaction. It is concluded that ducks are susceptible to NDV and virulent strain of NDV caused disease in ducks.


Resumo A doença de Newcastle (DN) é uma doença infecciosa, altamente contagiosa e letal de espécies aviárias. Considera-se que os patos são reservatórios ou portadores naturais do vírus da doença de Newcastle (VDN) e são resistentes a diferentes cepas de VDN. O presente estudo foi desenvolvido para avaliar a patogênese da DN em patos domésticos por meio de histopatologia, imuno-histoquímica (IHQ) e alterações bioquímicas séricas. Para este propósito, 80 patos foram criados por 42 dias e divididos em dois grupos A e B. Os patos do grupo A foram submetidos ao VDN a uma taxa de 0,1 ml de ELD50 (título viral de 107,32 / 100 µl) na segunda semana de idade, enquanto o Grupo B foi controle negativo. Esplenomegalia, atrofia do timo e lesão necrótica no rim foram observadas no 9º dia pós-infecção. Degeneração hepática e infiltração de células mononucleares foram observadas no proventrículo e intestino em patos infectados. Antígeno viral foi detectado em pulmões, intestino, proventrículo e órgãos linfoides de patos infectados por IHQ. Os valores de albumina e proteína total foram significativamente baixos no grupo A infectado em comparação com o grupo B. Os valores de ALT, AST e ALP foram significativamente altos no grupo A. No 5º e no 7º dia após a infecção, os esfregaços orofaríngeos foram negativos para VDN, enquanto os esfregaços cloacais foram positivos para VDN por meio da reação em cadeia da polimerase via transcriptase reversa. Conclui-se que os patos são suscetíveis ao VDN e à cepa virulenta de VDN que causou doenças em patos.


Subject(s)
Animals , Newcastle disease virus , Ducks , Newcastle Disease/diagnosis
16.
Arq. ciências saúde UNIPAR ; 27(2): 1010-1026, Maio-Ago. 2023.
Article in Portuguese | LILACS | ID: biblio-1425168

ABSTRACT

A Doença Renal Crônica (DRC) é uma importante redução da função renal que causa alterações no metabolismo dos indivíduos. Para acompanhar a progressão da DRC e prevenir possíveis complicações, foi realizada uma pesquisa para avaliar o perfil sociodemográfico, bioquímico e hematológico de pacientes com Insuficiência Renal Crônica (IRC) submetidos a hemodiálise. Esta pesquisa foi quantitativa, descritiva e transversal de caráter retrospectivo, realizada por meio da análise de dados secundários contidos nos prontuários dos pacientes. A coleta de dados ocorreu no Centro de Hemodiálise da cidade de Russas, no Ceará. A amostra foi constituída por 161 pacientes com DRC, sendo 63,35% do sexo masculino e 85,71% pardos, com uma idade média de 54,39 anos. Desses, 63,97% tinham entre 2 e 10 anos de tratamento e 57,76% possuíam ensino fundamental incompleto. 19,25% residiam em Russas. Resultados: Após a hemodiálise, os resultados mostraram 44 mg/dL de Ureia, 48,44% dos pacientes com valores normais. A hemoglobina e hematócrito médios foram 11,8 g/dL e 33,7%, respectivamente, sendo que 63,35% tiveram valores reduzidos. 85,10% dos pacientes tiveram contagem de plaquetas normal, 72,04% níveis adequados de ferro e albumina, 52,79% tiveram níveis elevados de ferritina, 23,61% redução de transferrina e níveis lipídicos satisfatórios. 79,50% apresentaram níveis séricos de potássio dentro da normalidade, 12,42% de fósforo acima do normal, 85,09% de cálcio dentro dos valores normais, 39,13% de PTHi normais e 86,33% de glicose dentro dos valores considerados normais. Com base nos resultados, concluiu-se que todos os pacientes em tratamento hemodialítico apresentam diversas alterações em decorrência da DRC e do próprio processo de tratamento. Portanto, a realização de exames para avaliar ou monitorar possíveis complicações da IRC é essencial para criar estratégias e intervenções mais eficazes, que melhorem a assistência prestada a esses pacientes e, consequentemente, da qualidade e expectativa de vida dos mesmos.


Chronic Kidney Disease (CKD) is an important reduction in kidney function that causes changes in the metabolism of individuals. To monitor the progression of CKD and prevent possible complications, a survey was carried out to assess the sociodemographic, biochemical and hematological profile of patients with Chronic Renal Failure (CRF) undergoing hemodialysis. This research was quantitative, descriptive and cross-sectional with a retrospective character, carried out through the analysis of secondary data contained in the patients' medical records. Data collection took place at the Hemodialysis Center in the city of Russas, Ceará. The sample consisted of 161 patients with CKD, 63.35% male and 85.71% brown, with an average age of 54.39 years. Of these, 63.97% had between 2 and 10 years of treatment and 57.76% had incomplete primary education. 19.25% resided in Russas. Results: After hemodialysis, the results showed 44 mg/dL of Urea, 48.44% of patients with normal values. Average hemoglobin and hematocrit were 11.8 g/dL and 33.7%, respectively, with 63.35% having reduced values. 85.10% of the patients had normal platelet counts, 72.04% had adequate levels of iron and albumin, 52.79% had high levels of ferritin, 23.61% had reduced transferrin and satisfactory lipid levels. 79.50% had serum levels of potassium within the normal range, 12.42% of phosphorus above normal, 85.09% of calcium within normal values, 39.13% of PTHi normal and 86.33% of glucose within the values considered normal. Based on the results, it was concluded that all patients on hemodialysis have several changes due to CKD and the treatment process itself. Therefore, carrying out tests to assess or monitor possible complications of CRF is essential to create more effective strategies and interventions that improve the care provided to these patients and, consequently, their quality and life expectancy.


La Enfermedad Renal Crónica (ERC) es una reducción importante de la función renal que provoca cambios en el metabolismo de los individuos. Para monitorizar la evolución de la ERC y prevenir posibles complicaciones, se realizó una encuesta para evaluar el perfil sociodemográfico, bioquímico y hematológico de los pacientes con Insuficiencia Renal Crónica (IRC) en hemodiálisis. Esta investigación fue cuantitativa, descriptiva y transversal con carácter retrospectivo, realizada a través del análisis de datos secundarios contenidos en las historias clínicas de los pacientes. La recolección de datos ocurrió en el Centro de Hemodiálisis de la ciudad de Russas, Ceará. La muestra estuvo constituida por 161 pacientes con ERC, 63,35% del sexo masculino y 85,71% pardos, con una edad media de 54,39 años. De estos, 63,97% tenían entre 2 y 10 años de tratamiento y 57,76% tenían primaria incompleta. El 19,25% residía en Russas. Resultados: Posterior a la hemodiálisis los resultados arrojaron 44 mg/dL de Urea, 48,44% de los pacientes con valores normales. La hemoglobina y el hematocrito medios fueron 11,8 g/dl y 33,7 %, respectivamente, con un 63,35 % con valores reducidos. El 85,10% de los pacientes presentaba plaquetas normales, el 72,04% presentaba niveles adecuados de hierro y albúmina, el 52,79% presentaba niveles elevados de ferritina, el 23,61% presentaba transferrina reducida y niveles satisfactorios de lípidos. El 79,50% presentaba niveles séricos de potasio dentro de la normalidad, el 12,42% de fósforo por encima de lo normal, el 85,09% de calcio dentro de los valores normales, el 39,13% de PTHi normal y el 86,33% de glucosa dentro de los valores considerados normales. Con base en los resultados, se concluyó que todos los pacientes en hemodiálisis tienen varios cambios debido a la ERC y al propio proceso de tratamiento. Por tanto, la realización de pruebas para evaluar o monitorizar las posibles complicaciones de la IRC es fundamental para crear estrategias e intervenciones más eficaces que mejoren la atención a estos pacientes y, en consecuencia, su calidad y esperanza de vida.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Patients/statistics & numerical data , Health Profile , Renal Dialysis/statistics & numerical data , Renal Insufficiency, Chronic/epidemiology , Kidney Diseases/epidemiology , Serology , Biochemistry , Medical Records/statistics & numerical data , Cross-Sectional Studies/methods , Creatinine , Data Analysis , Hematology
17.
rev. psicogente ; 26(50)dic. 2023.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1536984

ABSTRACT

Objetivo: El propósito de este estudio es establecer los mecanismos de la actividad Bioquímica a través del Estrés Psicológico que influyen en la enfermedad del vitiligo desde una perspectiva neurocientifica, mediante una revisión sistemática de la literatura de los últimos 5 años. Método: La búsqueda sistemática se llevó a cabo en las bases de datos Scopus, PubMed y Web of Science para identificar los estudios que describan la enfermedad de Vitiligo, el estrés psicológico y la bioquímica del cerebro, se establecieron criterios de inclusión y exclusión para la selección de los artículos, así mismo, se evaluó su calidad y pertinencia. Resultados: La revisión sistemática arrojó 15.503 artículos, 70 fueron preseleccionados de acuerdo a lo informado en el resumen y validados para lectura completa. Para la síntesis narrativa se utilizaron 23 artículos científicos. Conclusión: La enfermedad de vitiligo guarda una estrecha relación con el estrés psicológico y está acompañado de un desequilibrio bioquímico que conduce a un proceso inflamatorio y destructivo de los melanocitos de la piel.


Objective: The purpose of this study is to establish the mechanisms of biochemical activity through psychological stress that influence vitiligo disease from a neuroscientific perspective, through a systematic review of the literature of the last 5 years. Method: Systematic search was carried out in the Scopus, PubMed and Web of Science databases to identify studies describing Vitiligo disease, psychological stress and brain biochemistry, inclusion and exclusion criteria were established for the selection of articles, their quality and relevance were evaluated. Results: The systematic review yielded 15,503 articles, of which 70 were pre-selected as reported in the summary and validated for full reading. For the narrative synthesis 23 scientific articles were used. Conclusion: Vitiligo disease is closely related to psychological stress and is accompanied by a biochemical imbalance that leads to an inflammatory and destructive process of skin melanocytes.

18.
Medicina (Ribeirao Preto, Online) ; 56(3)nov. 2023. tab, ilus
Article in English | LILACS | ID: biblio-1551202

ABSTRACT

Objective: Correlate inflammatory mediators and biochemical parameters in patients with active pulmonary tuberculosis (TB) treated at a public hospital in São Luís, MA. Methods: This is a case-control study of patients with a positive diagnosis of active pulmonary TB. Serum samples from patients and the control group were collected for the clinical trials, and epidemiological data were collected through medical records and interviews. The control group consisted of healthy volunteers with no previous contact with TB cases, matched by age and sex to the clinical group. To measure inflammatory cytokines, we used the Human IL-6 ELISA Set and Human IFN-γ ELISA Set kits. Oxidative stress was measured by quantification of thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO). In biochemistry, the levels of uric acid, antistreptolysin "O" (AEO), alanine aminotransferase (ALT), amylase, aspartate aminotransferase (AST), calcium, total cholesterol, gamma-glutamyl transferase (Gamma GT), glucose, alkaline phosphatase, high-density lipoprotein (HDL), C-reactive protein (CRP) and triglycerides were measured. Results: The clinical group consisted of 53 patients. There was a substantial decrease in IFN-γ (p<0.0001) and a significant increase in IL-6 (p<0.0001). TBARS production increased significantly (p= 0.0414). There was no significant difference in NO production (p= 0.3194). In biochemistry, there was a significant increase in ALT (p= 0.0072), AST (p= 0.0016), Gamma GT (p= 0.0011), alkaline phosphatase (p<0.0001), CRP (p<0. .0001) and triglycerides (p= 0.0343), and a significant decrease in calcium (p<0.0001). A significant positive correlation was found between IL-6 and IFN-γ (p= 0.0448), as well as AST and ALT (p<0.0001); CRP and gamma GT (p<0.0001); Gamma GT and ALT (p= 0.0016); Gamma GT and AST (p=0.0004); triglycerides and cholesterol (p= 0.0002); alkaline phosphatase and gamma GT (p<0.0001); CRP and alkaline phosphatase (p<0.0001); triglycerides and calcium (p= 0.0121); cholesterol and calcium (p= 0.0261); glucose and cholesterol (p= 0.0373); and triglycerides and glucose (p= 0.0127) in biochemistry, with a significant negative correlation between glucose and uric acid (p= 0.0092); and CRP and HDL (p=0.0037). The correlation between inflammatory mediators and biochemical markers was positive between IL-6 and gamma GT (p= 0.0011); IL-6 and CRP (p<0.0001); IL-6 and alkaline phosphatase (p=0.0076); and NO and triglycerides (p= 0.0016), and significant negative correlation between IFN-γ and cholesterol (p= 0.0171) and TBARS and cholesterol (p= 0.0138). Conclusion: Immunosuppression of IFN-γ activity was observed. A correlation was found between IL-6 and inflammatory biochemical markers, indicating damage and injury caused by M. tuberculosis (AU).


Objetivo: Correlacionar mediadores inflamatórios e parâmetros bioquímicos em pacientes com tuberculose (TB) pulmonar ativa atendidos em um hospital público, em São Luís, MA. Métodos: Trata-se um caso-controle de pacientes com diagnóstico positivo para TB pulmonar ativa. Amostras de soro dos pacientes e grupo controle foram coletadas para os experimentos clínicos e os dados epidemiológicos foram coletados por meio de prontuários e entrevistas. O grupo controle foi formado por voluntários saudáveis sem contato prévio com casos de TB, pareados com idade e sexo ao grupo clínico. Para dosar citocinas inflamatórias, utilizaram-se os kits Human IL-6 ELISA Set e Human IFN-γ ELISA Set. Mediu-se o estresse oxidativo pela quantificação das espécies reativas do ácido tiobarbitúrico (TBARS) e óxido nítrico (ON). Na bioquímica, mediram-se os níveis de ácido úrico, anti-estreptolisina-O (AEO), alanina aminotransferase (ALT), amilase, aspartato aminotransferase (AST), cálcio, colesterol total, gama glutamil transferase (Gama GT), glicose, fosfatase alcalina, lipoproteína de alta densidade (HDL), proteína C reativa (PCR) e triglicerídeos. A análise estatística foi realizada pelo software Graph Pad Prism 8, com p<0,05 significativo. Re -sultados: O grupo clínico foi formado por 53 pacientes. Houve uma diminuição significativa de IFN-γ (p<0,0001), e aumento significativo de IL-6 (p<0,0001). A produção de TBARS aumentou significativamente (p= 0,0414). Não houve diferença significativa na produção de ON (p= 0,3194). Na bioquímica, houve aumento significativo em ALT (p= 0,0072), AST (p= 0,0016), gama GT (p= 0,0011), fosfatase alcalina (p<0,0001), PCR (p<0,0001) e triglice-rídeos (p= 0,0343), e diminuição significativa de cálcio (p<0,0001). Encontrou-se correlação positiva significativa entre IL-6 e IFN-γ (p= 0,0448), assim como AST e ALT (p<0,0001); PCR e gama GT (p<0,0001); gama GT e ALT (p= 0,0016); gama GT e AST (p= 0,0004); triglicerídeos e colesterol (p= 0,0002); fosfatase alcalina e gama GT (p<0,0001); PCR e fosfatase alcalina (p<0,0001); triglicerídeos e cálcio (p= 0,0121); colesterol e cálcio (p= 0,0261); glicose e colesterol (p= 0,0373); e triglicerídeos e glicose (p= 0,0127) na bioquímica, sendo negativa significativa entre glicose e ácido úrico (p= 0,0092); e PCR e HDL (p= 0,0037). A correlação entre marcadores infla-matório e bioquímicos foi positiva entre IL-6 e gama GT (p= 0,0011); IL-6 e PCR (p<0,0001); IL-6 e fosfatase alcalina (p= 0,0076); e ON e triglicerídeos (p= 0,0016), e negativa significativa entre IFN-γ e colesterol (p= 0,0171) e TBARS e colesterol (p= 0,0138). Conclusões: Observou-se imunossupressão da atividade de IFN-γ. Encontrou-se correlação entre IL-6 e marcadores bioquímicos inflamatórios, indicando dano e lesão causados por M. tuberculosis (AU).


Subject(s)
Humans , Male , Female , Biochemistry , Cytokines , Inflammation Mediators
19.
Int J Pharm Biol Arch ; 2023 Jul; 14(3): 119-122
Article | IMSEAR | ID: sea-231076

ABSTRACT

The non-steroidal anti-inflammatory medicine indomethacin causes pathogenesis that involves reactive oxygen species and lipid peroxidation. This study examined how ethanol and indomethacin affected heat-stressed rats’ hematological, histopathological, and antioxidant functions. Three groups of 18 male albino rats were created. Group A acted as the standard control group and received saline as usual for 15 days. Group B was given 0.2 mL of ethanol orally each day. In group C, indomethacin 10 mg/kg/day was administered orally once daily. Rats given ethanol and indomethacin had significantly lower levels of red blood cells, packed cell volume, and hemoglobin compared to the control group. White blood cell (WBC), lymphocyte (LYM), and neutrophil (NEUT) levels were significantly altered by the addition of ethanol and indomethacin, with indomethacin administration significantly increasing WBC and NEUT in comparison to ethanol administration. Compared to the stress control group, the levels of malondialdehyde and superoxide dismutase were significantly elevated and decreased, respectively, in the rats supplemented with ethanol and indomethacin. In addition, rats given ethanol and indomethacin showed significantly reduced catalase, glutathione, and glutathione peroxidase activities. The stomachs of rats given indomethacin and ethanol treatment underwent histological analysis, which revealed varying degrees of architectural deformities. These results imply that supplementation with ethanol and indomethacin may harm rats under heat stress.

20.
Article | IMSEAR | ID: sea-237855

ABSTRACT

In the present study, 88 dogs were diagnosed with hepatic disorders based on clinical manifestations, haemato-biochemistry and diagnostic Imaging. All the cases exhibited clinical manifestations of inappetance and anorexia, vomition, anemia, diarrhea, pyrexia, lethargy, icterus, abdominal pain, emaciation, ascites, weight gain,weight loss, respiratory distress, limb edema, nervous signs, polyuria and polydipsia. Significantly decreased Hb and TEC with elevated TLC and neutrophil count were common hematological abnormalities. Similarly, elevated activity of ALT, AST, ALP, GGT, total bilirubin, direct bilirubin and globulin with decreased levels of total protein, albumin, glucose and serum electrolytes (sodium, potassium and chloride) were common biochemical findings recorded in dogs affected with hepatic disorders. Ground glass appearance, hepatomegaly and normal liver size were the common radiographic findings Based on ultrasonographic evaluation carried out on the suspected cases of hepatic disorders, revealed changes in size (normal reducedand enlarged), echogenecity, (hyper, hypo andmixed echogenicity), margins (sharp, rounded and irregular), portal and hepatic veins (Normal and inapparent), hypoechoic masses and anechoic fluid.

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