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1.
Rev. Flum. Odontol. (Online) ; 2(67): 88-105, mai-ago.2025. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1573152

ABSTRACT

Este trabalho, partindo do tema elaborado, levantou o seguinte problema: As propriedades e vantagens estudadas nas membranas de Hidrogel, se faz desse material eficaz no tratamento dos tecidos periodontais na doença periodontal. Sendo assim, o objetivo deste trabalho foi realizar um levantamento bibliográfico sobre as propriedades e vantagens do uso de membranas de Hidrogel na Regeneração Óssea Guiada nos casos da perda óssea e a recessão gengival associada à Doença Periodontal. O uso de membrana para Regeneração Óssea Guiada (ROG) é um componente essencial do tratamento de doenças periodontais e na regeneração óssea. Neste caso, discutiremos as propriedades dos hidrogéis e seus benefícios e limitações nessa área. Apesar dos desafios significativos existentes, a regeneração óssea baseada em hidrogel é uma grande promessa para o futuro tratamento de doenças e defeitos relacionados aos ossos. Com uma compreensão aprofundada os hidrogéis serão, sem dúvida, uma ferramenta poderosa para o tratamento clínico de defeitos ósseos no futuro.


This study, based on the topic elaborated, raised the following problem: The properties and advantages studied in Hydrogel membranes and if this material can be effective in the treatment of periodontal tissues in periodontal disease. The objective of this work was to perform a bibliographic survey on the properties and advantages of using Hydrogel membranes in Guided Bone Regeneration in cases of bone loss and gingival recession associated with Periodontal Disease. The use of Guided Bone Regeneration (GBR) membrane is an essential component of the treatment of periodontal diseases and bone regeneration. In this case, we discussed the properties of hydrogels and their benefits and limitations in this area. Despite significant challenges, the hydrogel-based bone regeneration holds great promise for the future treatment of bone-related diseases and defects. With in-depth understanding, hydrogels will undoubtedly be a powerful tool for clinical treatment of bone defects in the future.


Subject(s)
Periodontal Diseases , Bone Regeneration , Hydrogels , Membranes
2.
Odovtos (En línea) ; 26(3): 161-174, Sep.-Dec. 2024. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1582851

ABSTRACT

Abstract Guided Bone Regeneration (GBR) is the choice of treatment for improving the horizontal and vertical bone volume through bone grafting. GBR membranes work on the principle of preventing epithelial migration into the defect space while maintaining the space for cell migration and differentiation at the defect site. Hydroxyapatite has been commonly used as a bone graft for infrabony defects. The study was conducted at the Department of Biomaterials at Saveetha Dental College. GBR membrane was prepared and its material characterization was done using Scanning Electron Microscope (SEM), Energy Dispersive X-ray (EDX) analysis, Fourier Transform Infrared Radiation (FTIR), and Confocal Analysis. The developed GBR membrane revealed SEM properties conducive to cell attachment. EDX and FTIR analysis showed the successful development of the collagen-gelatin-hydroxyapatite membrane. Cell culture and confocal analysis revealed excellent biocompatibility with a homogenous layer of viable cells. The developed composite GBR membrane is a biogenic membrane with relevant biomineralization potential that should be applied for GBR applications.


Resumen La regeneración ósea guiada (GBR) es el tratamiento de elección para mejorar el volumen óseo horizontal y vertical mediante injertos óseos. Las membranas GBR funcionan según el principio de prevenir la migración epitelial hacia el espacio del defecto. La hidroxiapatita se ha utilizado habitualmente como injerto óseo para defectos infraóseos. El estudio se realizó en el Departamento de Biomateriales del Saveetha Dental College. Se preparó un scaffold o andamio, el cual se caracterizó mediante microscopio electrónico de barrido (SEM), análisis de rayos X de dispersión de energía (EDAX), radiación infrarroja por transformada de Fourier (FTIR) y análisis confocal. El andamio desarrollado reveló propiedades propicias para la unión celular. Los análisis EDAX y FTIR mostraron el desarrollo exitoso de la membrana de colágeno-gelatina-hidroxiapatita. El cultivo celular y el análisis confocal revelaron una excelente biocompatibilidad con una capa homogénea de células viables. El andamio desarrollado es una membrana biogénica con un potencial de biomineralización relevante que puede utilizarse para aplicaciones GBR.

3.
Rev. biol. trop ; Rev. biol. trop;72(1): e53238, ene.-dic. 2024. tab, graf
Article in English | SaludCR, LILACS | ID: biblio-1559323

ABSTRACT

Abstract Introduction: Evidence suggests that herbivores, such as peccaries, shape vegetation structure and diversity through predation, trampling, dispersal, and rooting behavior. Objective: To evaluate the impact of peccaries (Dycotiles tajacu) on the understory vegetation of the tropical rainforest in the Nogal-La Selva Local Biological Corridor, Costa Rica, comparing a site with the absence of peccaries to another with the presence of these animals. Methodology: From June to November 2021, 20 experimental exclusions and 20 free access plots, each measuring 2 m2 were used to quantify herbivory, the number of leaf blades, damaged leaves, healthy leaves, sapling height, and fallen biomass at both sites. Results: A higher sapling density was found in the Nogal Reserve, but a lower sapling diversity, while in La Selva there was a higher sapling diversity, but a lower density of seedlings. Herbivory and sapling height in La Selva exceeded those in Nogal. The exclusion of peccaries reduced seedling damage but did not affect the dynamics of fallen biomass. Conclusion: For the design, implementation, and evaluation of the effectiveness of biological corridors, it is crucial to consider plant-animal interactions to enhance the flow of ecological processes through functional and structural connectivity, analyzed from interactions such as those presented in this paper.


Resumen Introducción: Existe evidencia que herbívoros, como los saínos, dan forma a la estructura y diversidad de la vegetación a través del comportamiento de depredación, pisoteo, dispersión y enraizamiento. Objetivo: Evaluar el impacto de los saínos (Dycotiles tajacu) en la vegetación del sotobosque del bosque tropical húmedo en el Corredor Biológico Local Nogal-La Selva, Costa Rica, en un sitio con ausencia y en otro con presencia de saínos. Métodos: De junio a noviembre de 2021 se utilizaron 20 exclusiones experimentales y 20 parcelas de acceso libre de 2 m2, se cuantifico la herbivoría, número de láminas foliares, hojas dañadas, hojas sanas, altura de brinzales y biomasa caída en ambos sitios. Resultados: Se encontró una mayor densidad de brinzales en Reserva Nogal pero una menor diversidad, contrario en La Selva donde se encontró una mayor diversidad de brinzales, pero una menor densidad de plántulas. La herbivoría y la altura de brinzales en La Selva fue mayor que en Nogal. La exclusión de los saínos disminuyó el daño a las plántulas, pero no afectó la dinámica de la biomasa caída. Conclusión: Es necesario contemplar para el diseño, implementación y evaluación de la efectividad de corredores biológicos, las interacciones planta-animal, para potencializar el flujo de procesos ecológicos mediante la conectividad funcional y estructural, analizada a partir de interacciones como las presentadas en este trabajo.


Subject(s)
Animals , Artiodactyla , Forests , Animal Distribution , Tropical Ecosystem , Costa Rica
4.
Article | IMSEAR | ID: sea-241669

ABSTRACT

Gelatin methacrylate (GelMA) has gained signicant attention in dentistry as a versatile biomaterial with broad applications in tissue engineering and regenerative therapies. This review explores the role of GelMA in dental applications, emphasizing its unique properties such as biocompatibility, tunable mechanical characteristics, and photocrosslinkability. GelMA serves as an ideal scaffold for the regeneration of dental tissues, including dental pulp, periodontal ligaments, and alveolar bone, promoting cell adhesion, proliferation, and differentiation. The material's ability to be functionalized with bioactive molecules further enhances its regenerative potential. Additionally, its application in 3D bioprinting and drug delivery systems holds promise for creating patient-specic treatments and improving outcomes in conditions like periodontitis and pulpitis. This review also addresses the future perspectives in highlight on the opportunities for advancing GelMa hydrogels as innovative materials in modern dentistry. Overall, GelMA represents a promising biomaterial in the eld of dentistry, with ongoing research focusing on overcoming current limitations and expanding its clinical applications.

5.
Acta odontol. latinoam ; Acta odontol. latinoam;37(2): 123-133, Sept. 2024. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1581562

ABSTRACT

ABSTRACT Melatonin (MLT) is a hormone that can stimulate bone formation and inhibit bone resorption, among other functions. Aim To evaluate the effect on new bone formation of MLT applied locally to critical defects created in the calvaria of rats, compared to the effect of Bio-Oss® xenogeneic bone substitute (BO), by analyzing histomorphometry, microtomography and gene expression. Materials and Method Two critical defects (5.0 mm in diameter) were created in the calvaria of 36 adults male Wistar rats. The rats were divided randomly into two groups: a test group, in which one of the defects was filled with MLT, and the other with MLT with Bio-Oss® (MLTBO), and a control group, in which one of the defects was filled only with the clot (C), and the other with BO. The rats were euthanized 30 days after surgery. Samples of the calvaria containing the critical defects were collected for analysis by histomorphometry, microtomography, and the expression of the genes for type I collagen (COL-I), osteopontin (OPN) and bone morphogenetic protein 2 (BMP-2). Results A qualitative improvement was observed in bone healing when MLT was used, though there was no statistical difference in the quantification of newly formed bone (p>0.05). Micro-CT showed that bone volume was significantly smaller in absence of BO (p=0.006). Bone trabeculae thickness (p=0.590) and number (p=0.150) were not significantly affected by MLT. Regarding the expression of the genes COL-I, OPN and BMP-2, no significant differences were observed between the MLT, BO and MLTBO groups. Conclusion Topical application of MLT resulted in a qualitative improvement in bone healing, although it did not affect bone formation quantitatively. In the absence of BO, less bone volume and less bone trabecular thickness were observed.


RESUMO A melatonina (MLT) é um hormônio sintetizado e secretado pela glândula pineal, e que, dentre outras atividades e funções, tem capacidade de estimular a formação e inibir a reabsorção óssea. Objetivo avaliar o efeito da aplicação local do hormônio MLT na neoformação óssea, em defeitos críticos produzidos na calvária de ratos, por meio de análise histomorfométrica, microtomográfica e de biologia molecular, comparando-a com um substituto ósseo de origem xenogênica (Bio-Oss®). Materiais e Método foram utilizados 36 ratos Wistar machos adultos, nos quais foram produzidos dois defeitos críticos de 5,0 mm de diâmetro cada, que receberam diferentes tratamentos alocados de forma randomizada: no grupo controle, os animais tiveram um dos defeitos preenchidos apenas com coágulo sanguíneo (C) e o outro com substituto ósseo xenógeno (Bio-Oss®, BO); no grupo teste, um dos defeitos foi preenchido apenas com MLT e, o outro, recebeu a associação da MLT ao material sintético xenógeno (MLTBO). Todos os animais foram eutanasiados após 30 dias do pós-operatório. As amostras das calotas contendo os defeitos críticos foram coletadas para análises histomorfométricas, microtomográficas e da expressão gênica de colágeno do tipo I (COL-I), osteopontina (OPN) e proteína óssea morfogenética 2 (BMP-2), por meio de PCR em tempo real. Resultados Após análise dos dados pode-se observar que não houve diferença estatística na quantificação de neoformação óssea (p>0.05), porém, melhora qualitativa foi observada na cicatrização, quando a MLT foi utilizada. Quanto aos par®metros microtomográficos, foi observado que com ou sem MLT, o volume ósseo foi significativamente menor na ausência de BO. A espessura (p = 0,590) e número (p = 0,150) de trabéculas não foram significativamente afetados pelo uso da MLT. Quanto à expressão gênica de COL-I, OPMN e BMP-2, não foram observadas diferenças significativas entre os grupos MLT, BO e MLTBO. Conclusão conclui-se que a aplicação tópica de MLT, associada ou não ao BO não afetou quantitativamente a neoformação óssea, porém resultou em uma melhora qualitativa na cicatrização. Adicionalmente, na ausência de BO foi observada menor volume ósseo e menor espessura das trabéculas.

6.
Acta odontol. latinoam ; Acta odontol. latinoam;37(2): 151-161, Sept. 2024. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1581565

ABSTRACT

ABSTRACT This study analyzed a recently developed bone substitute biomaterial made of chitosan-xanthanhydroxyapatite- graphene oxide (CXHAG). The CXHAG particles underwent in vitro structural and morphological characterization, and in vivo testing with or without osteogenic conditioned medium from mesenchymal stem cells. Aim The aim of this study was to determine whether the CXHAG novel biomaterial, supplemented with conditioned medium from mesenchymal stem cells, could be useful for bone regeneration. Materials and Method For the in vitro study, cells were incubated with 20mg of CXHAG granules for 24 hours and a MTT assay was performed to tests for cytotoxicity. For the in vivo study, critical size calvarial bone defects were created in twenty-five rats. One animal had the defect unfilled (Control Group-CG) and was euthanized after 42 days. Twelve rats received the CXHAG particles (Group 1-G1) and the other twelve received the CXHAG particles supplemented with the conditioned medium (Group 2-G2). All G1/G2 grafts were covered with a CXHAG membrane. G1/G2 animals were euthanized after 14 days (T1) or 42 days (T2). The specimens were processed and histologically evaluated. Results SEM analysis of the CXHAG particles showed granules of 300-400μm, with a rough irregular surface. They were not cytotoxic to dental pulp stem cells in vitro. The CG specimen showed loose immature connective tissue and no bone formation at the center of the defect. G1 and G2 presented remnant biomaterial particles at both time points, but only G2 had bone formation at the center of the defect. Conclusions The conditioned medium had a positive effect on bone regeneration in rat calvarial critical size defects when associated with the novel bone substitute biomaterial.


RESUMO Este estudo analisou um biomaterial substituto ósseo recentemente desenvolvido feito de óxido de quitosana- xantana-hidroxiapatita-grafeno (CXHAG). As partículas CXHAG observaram caracterização estrutural e morfológica in vitro. Foi testado in vivo, com ou sem meio condicionado osteogênico de células-tronco mesenquimais. Objetivo O objetivo deste estudo foi determinar se o novo biomaterial CXHAG, suplementado com meio condicionado de células-tronco mesenquimais, poderia ser útil para a regeneração óssea. Materiais e Método Para o estudo in vitro, as células foram incubadas com 20mg de grânulos de CXHAG por 24 horas e foi realizado ensaio de MTT para verificar a citotoxicidade. Para o estudo in vivo, foram criados defeitos ósseos de tamanho crítico na calvária em vinte e cinco ratos. Um animal teve o defeito não preenchido (Grupo Controle - GC) e foi eutanasiado após 42 dias. Doze ratos receberam as partículas CXHAG (Grupo 1 - G1) e os outros doze receberam as partículas CXHAG suplementadas com o meio condicionado (Grupo 2 - G2). Todos os enxertos G1/G2 foram cobertos com membrana CXHAG. Os animais do G1/G2 foram eutanasiados após 14 dias (T1) ou 42 dias (T2). Os espécimes foram processados e avaliados histologicamente. Resultados A análise SEM das partículas CXHAG mostrou grânulos de 300-400μm, com superfície áspera e irregular. Eles não foram citotóxicos para células-tronco da polpa dentária in vitro. As amostras CG mostraram tecido conjuntivo imaturo frouxo e nenhuma formação óssea no centro do defeito. G1 e G2 apresentaram partículas remanescentes de biomateriais em ambos os momentos, mas apenas G2 apresentou formação óssea no centro do defeito. Conclusões O meio condicionado teve repercussões positivas na regeneração óssea em defeitos críticos de calvária de ratos quando associado ao novo biomaterial substituto ósseo.

7.
Article | IMSEAR | ID: sea-234196

ABSTRACT

Compared to typical bandaging materials, fish skin bandages have shown great promise as a wound treatment option. Fish skin is abundant, biocompatible, and naturally antibacterial, which makes it an ideal material for wound dressings. Moreover, fish skin shares a structure with human skin, which promotes improved integration with the wound bed and helps injured tissue regenerate. Fish skin bandages have emerged as a possible option for wound healing due to their unique biological features. This study examines the efficacy of fish skin bandages in wound treatment. Methods: This article attempts to understand the efficacy and mechanisms of action of fish skin bandages in encouraging wound healing by analyzing existing research, describing the biological makeup of fish skin, and investigating current investigations. Results: The results show that fish skin bandages have anti-inflammatory, antibacterial, and tissue-regenerative capabilities, making them an appealing solution for a variety of wounds Conclusion: The use of fish skin in wound treatment improves environmental sustainability by recycling fish waste. However, further study is needed to improve the manufacture, standardization, and therapeutic use of fish skin bandages.

8.
Article | IMSEAR | ID: sea-237677

ABSTRACT

The primary treatment for chronic liver disease (CLD) involves dietary and non-dietary interventions. However, when CLD progresses to end-stage liver disease, liver transplantation becomes the only viable option, despite challenges such as donor shortages, transplant rejection, immunosuppressive drug complications, and high costs. To address the urgent need for post-transplantation liver regeneration, the growing focus is emphasized on the stimulation of liver regeneration by pharmacological intervention, even in non-transplanted individuals or those with early liver complications. The objective of this work was to activate the Wnt/?-catenin signaling pathway by blocking GSK-3? to enhance the regeneration of hepatocytes and the liver. A partial hepatectomy was performed on 44 rats, with the animals divided into six groups and assessed from postoperative days 1 to 8. The effects of the GSK-3? inhibitor CHIR99021 (6.25 mg/kg/bw) on hepatocyte regeneration and hepatoprotection were evaluated using biochemical, histopathological methods, and docking studies. It was indicated that both single and repeated doses of CHIR99021 notably improved lipid profiles, liver function, and reduced oxidative stress in partial hepatectomized rats on the third and seventh day as compared to those without drug treatment. Histopathological assessments confirmed substantial hepatocyte regeneration after CHIR99021 treatment, supported by docking studies that showed notable binding interactions with important amino acids of the protein. Mitotic cell phase determination also validated the histopathological findings in different rodent groups. In conclusion, pharmacological stimulation of the Wnt/?-catenin pathway through GSK-3? inhibition with CHIR99021 demonstrated promising results in promoting hepatocyte regeneration, potentially offering a new treatment strategy for liver regeneration.

9.
Article | IMSEAR | ID: sea-241205

ABSTRACT

The study is the first attempt to highlight about callus induction and plantlet regeneration in rice variety ‘Nerica 3’ using mature seed as explant and using five regeneration media with plant growth regulators (BA, IAA, and 2,4-D) at different concentrations in other to establish the efficient indirect plant regeneration protocol. Among the five regeneration media tested, R3 medium (2 mg/L BA), showed greater percentage of plantlet regeneration (45.31%) followed by R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%), whereas, R4 medium showed the lowest (2 mg/L 2,4-D; 0%). These results will be used for genetic improvement programs of the variety ‘Nerica 3’.

10.
Rev. gastroenterol. Perú ; 44(3): 259-264, jul.-set. 2024. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1583898

ABSTRACT

ABSTRACT Background: One of the pathways involved in liver regeneration processes is TWEAK/Fn14 (tumor necrosis factor-related weak inducer of apoptosis/fibroblast growth factor-inducible 14), which has been proposed to act directly and selectively on hepatic progenitor cells; however, its role in the regeneration of steatotic liver metabolic dysfunction associated fatty liver disease has not been fully elucidated. Objective: To evaluate the behavior of Fn14 and its ligand TWEAK, as well as cellular stress signals as biochemical cues for possible liver regeneration in MAFLD. Materials and methods: A prospective study was carried out where the behavior of Fn14 and its ligand TWEAK, as well as cellular stress signals were observed as biochemical indications of a possible liver regeneration in a condition of tissue damage caused by excessive lipid accumulation. The expression of TWEAK, Fn14 and heat shock proteins in hepatic steatosis of non-alcoholic origin was assessed using immunohistochemistry and western blotting. Results: The histological classification of the tissues under study corresponded to microvesicular steatosis. We report a high level of expression of heat shock proteins in the cytoplasm. The expression of TWEAK and Fn14 in liver tissue affected by lipid accumulation was localized in the cytoplasm of hepatocytes, showing a higher intensity of reactivity for Fn14 compared to its ligand TWEAK. Conclusion: The expression of TWEAK/ Fn14 axis was positive suggesting reactivity of the signaling pathway in metabolic dysfunction associated fatty liver disease.


RESUMEN Antecedentes: Una de las vías implicadas en los procesos de regeneración hepática es el eje TWEAK/Fn14 (inductor débil de la apoptosis similar al factor de necrosis tumoral/factor de crecimiento de fibroblastos inducible 14), el cual se ha propuesto que actúa directa y selectivamente sobre las células progenitoras hepáticas; sin embargo, su papel en la regeneración del hígado esteatósico asociado a la enfermedad metabólica del hígado graso no ha sido completamente dilucidado. Objetivo: Evaluar el comportamiento de Fn14 y su ligando TWEAK, así como señales de estrés celular como indicadores bioquímicos de posible regeneración hepática en MAFLD. Materiales y métodos: Se llevó a cabo un estudio prospectivo donde se observó el comportamiento de Fn14 y su ligando TWEAK, así como señales de estrés celular como indicaciones bioquímicas de una posible regeneración hepática en una condición de daño tisular causado por acumulación excesiva de lípidos. La expresión de TWEAK, Fn14 y proteínas de choque térmico en esteatosis hepática de origen no alcohólico se evaluó mediante inmunohistoquímica y western blot. Resultados: La clasificación histológica de los tejidos estudiados correspondió a esteatosis microvesicular. Reportamos un alto nivel de expresión de proteínas de choque térmico en el citoplasma. La expresión de TWEAK y Fn14 en el tejido hepático afectado por acumulación de lípidos se localizó en el citoplasma de los hepatocitos, mostrando una mayor intensidad de reactividad para Fn14 en comparación con su ligando TWEAK. Conclusión: La expresión del eje TWEAK/Fn14 fue positiva, sugiriendo reactividad de la vía de señalización en la enfermedad metabólica del hígado graso asociada a disfunción metabólica.

11.
Article in English, Portuguese | LILACS-Express | LILACS | ID: biblio-1572255

ABSTRACT

Introduction: Photodynamic Therapy has been an important ally in tissue repair, with positive effects in the treatment of wounds and infections. Objective: To analyze the effects of the Healing Acceleration Methodology (MAC®) using propolis and toluidine blue on lower limb ulcers. Methods: Single-center, experimental, randomized and controlled study. Carried out between April 2021 and May 2022 at the outpatient clinic of the school clinic in a Brazilian capital. Patients were randomly assigned in a 1:1 ratio to receive photodynamic therapy (experimental group) or conventional dressing alone (control group). Patients over 18 years of age diagnosed with lower limb ulcers were included. Ten patients comprised the experimental group and 10 the control group. Patients were seen three times a week and monitored for two months. The primary outcome was the assessment of healing progress and the secondary outcome was the microscopic analysis of wound smears for the presence of cells and microorganisms. Results: In the experimental group, two wounds did not heal completely while in the control group, none of the 10 wounds healed completely during the 60-day follow-up. In the experimental group, 90% of patients did not have bacteria and fungi at the end of 30 days versus 40% in the control group. Conclusion: The present study showed that photodynamic therapy is a potential effective treatment in the healing process of lower limb ulcers. This can impact cost reduction and quality of life. Larger, well-designed studies are needed to corroborate the findings of the present study.


Introdução: a Terapia Fotodinâmica tem sido uma importante aliada no reparo tecidual, com efeitos positivos no tratamento de feridas e infecções. Objetivo: analisar os efeitos da Metodologia de Aceleração Cicatricial (MAC®) com uso da própolis e azul de toluidina em úlceras de membros inferiores. Método: estudo unicêntrico, experimental, randomizado e controlado. Realizado entre abril de 2021 e maio de 2022 no ambulatório da clínica escola em uma capital brasileira. Os pacientes foram distribuídos aleatoriamente em uma proporção de 1:1 para receber a terapia fotodinâmica (grupo experimental), ou apenas curativo convencional (grupo controle). Foram incluídos pacientes com idade superior a 18 anos com diagnóstico de úlceras de membros inferiores. Dez pacientes compuseram o grupo experimental e 10 o grupo controle. Os pacientes foram atendidos três vezes na semana e acompanhados por dois meses. O desfecho primário foi a avaliação do progresso de cicatrização e o secundário, a análise microscópica de esfregaço da ferida quanto a presença de células e micro-organismos. Resultados: no grupo experimental duas feridas não cicatrizaram completamente enquanto no grupo controle nenhuma das 10 feridas cicatrizou completamente durante o acompanhamento de 60 dias. No grupo experimental 90% dos pacientes não apresentavam bactérias e fungos ao final de 30 dias versus 40% do grupo controle. Conclusão: o presente estudo mostrou que a terapia fotodinâmica é um potencial tratamento eficaz no processo de cicatrização de úlceras de membros inferiores. Isto pode impactar em redução de custos e qualidade de vida. Estudos maiores e bem desenhados são necessários para corroborar os achados do presente estudo.

12.
Rev. cir. traumatol. buco-maxilo-fac ; 24(1): 37-43, jan.-mar. 2024. ilus
Article in Spanish | LILACS, BBO | ID: biblio-1567669

ABSTRACT

Introducción: El quiste nasopalatino es un quiste no odontogénico, ubicado dentro del conducto nasopalatino del maxilar, su diagnóstico en la mayoría de los casos es por hallazgo radiológico de rutina y en otros casos por la presencia de sintomatología; llegando a generar dolor, parestesia y signos visibles como deformaciones faciales. Descripción del caso: El objetivo de este artículo es la revisión de la literatura y presentación del caso de un paciente masculino de 38 años de edad, que acude a la consulta por presentar aumento de volumen en la maxila anterior, de dos años de evolución que presentó resultados favorables luego de la exéresis y colocación de injerto óseo. Consideraciones finales: El quiste nasopalatino representa menos del 5% de los quistes a nivel maxilar y su recurrencia es baja. Cuando se trata de un quiste de gran tamaño con destrucción del tejido óseo circundante, la mejor opción es la utilización de un injerto óseo dependiendo tanto de la biocompatibilidad, del sitio afectado y de los costos. Se considera como gold standard a la matriz ósea desmineralizada (DBX) debido a su capacidad de osteoinducción, osteoconducción y osteogénesis... (AU)


Introdução: O cisto nasopalatino é um cisto não odontogênico, localizado no interior do ducto nasopalatino da maxila, a lesao é descoberta na maior parte dos casos como um achado radiológico de rotina e em outros casos pela presença de sintomatologia; incluindo dor, parestesias e deformidades faciais. Descrição do caso: O objetivo deste artigo é revisar a literatura e apresentar o caso de um paciente masculino de 38 anos de idade que veio à clínica com um aumento volumétrico da região anterior da maxila, com uma evolução de dois anos, que apresentou resultados favoráveis após excisão e colocação de enxerto ósseo. Considerações finais: O cisto nasopalatino representa menos de 5% dos cistos maxilares e a recorrência é baixa. Quando se trata de uma grande lesão com destruição do tecido ósseo circundante, a melhor opção é a utilização de um enxerto ósseo dependendo da biocompatibilidade, do local afetado e dos custos. A matriz óssea desmineralizada (DBX) é considerado o material padrão para a reconstrução óssea da região afetada devido à sua capacidade de osteoindução, osteocondução e osteogénese... (AU)


Introduction: The nasopalatine cyst is a non-odontogenic cyst, located within the nasopalatine duct of the maxilla, its diagnosis in most cases is by routine radiological finding and in other cases by the presence of symptomatology; coming to generate pain, paresthesia and visible signs such as facial deformities. Case description: The objective of this article is to make a literature review and presentation of the case of a 38-year-old male patient, who comes to the consultation for presenting an increase in volume in the anterior maxilla, of two years of evolution that presented favorable results after excision and placement of bone graft. Final considerations: Nasopalatine cyst represents less than 5% of the cysts at maxillary level and its recurrence is low. When it is a large cyst with destruction of the surrounding bone tissue, the best option is the use of a bone graft depending on biocompatibility, the affected site and costs. Demineralized bone matrix (DBX) is considered the gold standard due to its capacity for osteoinduction, osteoconduction and osteogenesis... (AU)


Subject(s)
Humans , Male , Adult , Bone Matrix , Nonodontogenic Cysts , Bone Transplantation
13.
Int. j. morphol ; 42(3): 698-708, jun. 2024. ilus, tab
Article in English | LILACS | ID: biblio-1564638

ABSTRACT

SUMMARY: Tissue engineering aims to fabricate a scaffold that exhibits a suitable surface topography for a desired cellular response. Therefore, a study analyzing the characteristics of bone grafts is important for future research directions. This work aims to analyze the physical-chemical characteristics of commercially available bone grafts of human and bovine origin for dental use, using morphological analysis of the surface and chemical composition by variable pressure scanning electron microscope (VP-SEM) and energy-dispersive x-ray (EDX) spectrometry. In addition, pore diameter and surface area were analyzed by degassing method using a porosimeter, and particle size by laser diffraction. The analyzed allograft and xenograft particles differ in morphological characteristics and chemical composition. The allograft particles present a cuboidal and prismatic geometric morphology with angled edges and the absence of macropores. On the contrary, the xenograft particles present an irregular morphology with macropores in their structure. There is a statistically significant difference in C, P, and Ca between the xenograft and allografts (p < 0,05). The analyzed composition of allografts showed mainly the presence of C and O. In contrast, the composition of the xenograft was mainly Ca. These differences could influence the osteogenic properties of allografts and xenografts. This analysis provides basic information to understand the physicochemical properties of allografts and xenografts that facilitate cell-graft interaction.


La ingeniería de tejidos tiene como objetivo fabricar un andamio que muestre una topografía de superficie adecuada para una respuesta celular deseada. Por tanto, un estudio que analice las características de los injertos óseos es importante para futuros enfoques de investigación. Este trabajo tiene como objetivo analizar las características físico-químicas de injertos óseos de origen humano y bovino disponibles comercialmente para uso odontológico, mediante análisis morfológico de la superficie y composición química mediante microscopio electrónico de barrido de presión variable (VP-SEM) y x-dispersivo de energía. espectrometría de rayos (EDX). Además, el diámetro de los poros y el área superficial se analizaron mediante el método de desgasificación utilizando un porosímetro y el tamaño de las partículas mediante difracción láser. Las partículas de aloinjerto y xenoinjerto analizadas difieren en características morfológicas y composición química. Las partículas del aloinjerto presentan una morfología geométrica cúbica y prismática con bordes angulados y ausencia de macroporos. Por el contrario, las partículas de xenoinjerto presentan una morfología irregular con macroporos en su estructura. Existe una diferencia estadísticamente significativa en C, P y Ca entre el xenoinjerto y los aloinjertos (p < 0,05). La composición analizada de los aloinjertos mostró principalmente la presencia de C y O. Por el contrario, la composición del xenoinjerto fue principalmente Ca. Estas diferencias podrían influir en las propiedades osteogénicas de los aloinjertos y xenoinjertos. Este análisis proporciona información básica para comprender las propiedades fisicoquímicas de aloinjertos y xenoinjertos que facilitan la interacción célula-injerto.


Subject(s)
Humans , Animals , Cattle , Allografts/anatomy & histology , Allografts/chemistry , Spectrometry, X-Ray Emission , Bone Regeneration , Microscopy, Electron, Scanning , Porosity , Bone Transplantation , Heterografts/anatomy & histology , Heterografts/chemistry
14.
Rev. ADM ; 81(3): 164-169, mayo-jun. 2024. ilus
Article in Spanish | LILACS | ID: biblio-1566928

ABSTRACT

El odontólogo realiza de forma rutinaria procedimientos que generan lesiones en los tejidos duros y blandos, por lo que resulta importante que el profesional conozca los procesos normales de cicatrización y reparación. La cicatrización es un fenómeno fisiológico que se presenta en cualquier tejido vivo que ha sido lesionado, que tiene importantes componentes vasculares y celulares que llevan una secuencia específica y que dependiendo de la magnitud de la lesión, el tejido podrá regenerar o cicatrizar según sea el caso. Asimismo, existen patologías sistémicas específicas y locales capaces de retrasar el proceso normal de cicatrización. El objetivo del presente artículo es explicar el proceso normal de reparación tisular de los tejidos orales y periorales (AU)


The dentist routinely performs procedures that generate injuries to hard and soft tissues, so it is important that the professional knows the normal healing and repair processes. Cicatrization is a physiological phenomenon that occurs in any living tissue that has been injured that has important vascular and cellular components that carry a specific sequence and that, depending on the magnitude of the lesion, the tissue may regenerate or heal as the case may be. Likewise, there are specific systemic and local pathologies capable of delaying the normal healing process. The aim of this article is to explain the normal tissue repair process of oral and perioral tissues (AU)


Subject(s)
Humans , Wound Healing/physiology , Guided Tissue Regeneration , Mouth Mucosa/injuries , Bone Regeneration/physiology , Chronic Disease , Risk Factors , Granulation Tissue/physiopathology
15.
Medisan ; 28(3)jun. 2024.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1582753

ABSTRACT

Introducción: El procedimiento terapéutico con células mononucleares autólogas ha abierto una alternativa dentro del arsenal con que se cuenta para la reparación y regeneración de los defectos óseos máxilo-mandibulares derivados de procederes quirúrgicos, que se asocian con discapacidad y detrimento de la calidad de vida. Objetivo: Evaluar la evolución de los pacientes tratados con células mononucleares autólogas como terapia regenerativa ante defectos óseos máxilo-mandibulares. Métodos: Se realizó un estudio descriptivo transversal en los Servicios de Cirugía Máxilo-facial de la Clínica Estomatológica Provincial Docente y los Hospitales Provincial Docente Clinicoquirúrgico Saturnino Lora Torres, General Docente Juan Bruno Zayas Alfonso, Clinicoquirúrgico Docente Joaquín Castillo Duany de Santiago de Cuba, en el periodo de enero de 2017 a diciembre de 2019. Los pacientes incluidos en el estudio fueron sometidos a procedimientos quirúrgicos por enfermedades que afectaban la arquitectura ósea, implantándose células madre movilizadas con factor estimulante de colonias de granulocitos al finalizar el acto quirúrgico. Las variables investigadas fueron enfermedades quirúrgicas, complicaciones posquirúrgicas, tamaño del defecto óseo, evidencia radiográfica y evolución del tratamiento. Resultados: Entre los 6 y 12 meses posteriores a la cirugía y la terapia celular, todas las variables de respuesta al tratamiento probaron mejoras clínicas y radiológicas, observándose hueso de neoformación y aumento de la densidad ósea. Conclusiones: La regeneración de los defectos óseos, asociados a intervenciones quirúrgicas, mediante el implante de células mononucleares autólogas de sangre periférica suspendidas en plasma rico en plaquetas quedó evidenciada clínica y radiográficamente de forma satisfactoria.


Introduction: The therapeutic procedure with autologous mononuclear cells has opened an alternative within the arsenal available for repair and regeneration of the maxillomandibular bone defects derived from surgical procederes which are associated with disability and detriment to life quality. Objective: To evaluate the clinical course of patients treated with autologous mononuclear cells as regenerative therapy for maxillofacial bone defects. Methods: A cross-sectional descriptive study was carried out in the Maxillo Facial Surgery Services of the Teaching Provincial Dentistry Clinic and the Provincial Teaching Clinical Surgical Hospitals Saturnino Lora Torres, Teaching General Juan Bruno Zayas, Teaching Clinical Surgical Joaquin Castillo Duany, in Santiago de Cuba, from January, 2017 to December, 2019. The patients included in the study underwent surgical procedures due to diseases that affected bone architecture, implanting mobilized stem cells with granulocyte colony stimulating factor at the end of the surgical procedure. The variables investigated were surgical diseases, post-surgical complications, size of the bone defect, radiographic evidence and treatment evolution. Results: Six to 12 months after the surgery and cell therapy, all the response variables to the treatment proved clinical and radiological improvements, being observed bone neoformation and increase of bone density. Conclusions: Regeneration of bone defects, associated with surgical interventions, implanting autologous mononuclear cells of peripheral blood suspended in plasm rich in platelets was clinic and radiographically evidenced in a satisfactory way.

16.
Int. j. odontostomatol. (Print) ; 18(2): 189-193, jun. 2024. ilus
Article in Spanish | LILACS | ID: biblio-1564660

ABSTRACT

El tratamiento con implantes dentales hoy en día es un procedimiento clínico de rutina que permite rehabilitar a los pacientes con prótesis fijas. En este caso presentamos un tratamiento complejo de implantación inmediata del sector anterior con pérdida parcial de la cortical vestibular en el que se realizó una regeneración ósea guiada y provisionalización en un tiempo quirúrgico en un paciente con patología renal. Complementamos el estudio con una revisión de la efectividad de las técnicas utilizadas y las posibles respuestas celular asociadas a la patología renal.


Treatment with dental implants nowadays is a routine clinical procedure that allows patient rehabilitation with fixed prostheses. In this case we present a complex treatment of immediate implantation of the anterior sector with partial loss of the vestibular cortex, in which guided bone regeneration and provisionalization was performed in surgical time in a patient with kidney pathology. The study was complemented with a review of the effectiveness of the techniques used and the possible cellular responses associated with kidney pathology.


Subject(s)
Humans , Female , Middle Aged , Dental Implants , Dental Implants, Single-Tooth , Immediate Dental Implant Loading/methods , Bone Regeneration , Radiography, Dental , Treatment Outcome , Dental Implantation, Endosseous/methods , Esthetics, Dental , Hydronephrosis/pathology , Mouth Rehabilitation
17.
J. oral res. (Impresa) ; 13(1): 170-182, mayo 29, 2024. ilus, tab
Article in English | LILACS | ID: biblio-1566744

ABSTRACT

Background: Medication-related osteonecrosis of the jaw (MRONJ) is a rare, but significant adverse event primarily associated with the intake of antiresorptive and antiangiogenic medications. Although antiresorptive and antiangiogenic the-rapies improve life expectancy, particularly in cancer patients, MRONJ may hamper the patient's quality of life due to pain, discomfort, anxiety, depression, speech impairment, difficulty in swallowing and eating, frequent medical and dental evaluations and treatments, and the possibility of treatment discontinuation. Leukocyte­ and Platelet-rich Fibrin (L-PRF) is an autologous platelet aggregate that promotes wound healing by stimulating re-epithelialization, angiogenesis, and extracellular matrix production. Aim: The present systematic review aimed to compare the results in the published literature on whether L-PRF is an effective and predictable adjuvant to surgical debridement of necrotic bone for improving the healing efficacy in patients with MRONJ. Materials and Methods: The PubMed, Scopus, Cochrane, Science Direct, LILACS, and Web of Science databases were searched using the predetermined MeSH terms and eligibility criteria, and the search yielded a total of five articles. Two studies were retrospective, and three studies were case series. Results: Seventeen participants received a combination of surgical debridement, L-PRF membrane, and antibiotics. Complete wound healing was observed in 70% of the participants, and most of them healed without any complications. Conclusions: L-PRF as an adjuvant to surgical debridement of necrosed bone appears to have a positive association with the healing outcome in patients with MRONJ.


Introducción: La osteonecrosis mandibular relacionada con medicamentos (ONMRM) es un evento adverso raro pero significativo asociado principalmente con la ingesta de medicamentos antirresortivos y antiangiogénicos. Aunque las terapias antirresortivas y antiangiogénicas mejoran la esperanza de vida, particularmente en pacientes con cáncer, la ONMRM puede obstaculizar la calidad de vida del paciente debido a dolor, incomodidad, ansiedad, depresión, discapacidad del habla, dificultad para tragar y comer, evaluaciones y tratamientos médicos y dentales frecuentes, y la posibilidad de interrupción del tratamiento. La fibrina rica en plaquetas y leucocitos (L-PRF) es un agregado de plaquetas autólogo que promueve la curación de heridas al estimular la reepitelización, la angiogénesis y la producción de la matriz extracelular. Objetivo: La presente revisión sistemática tuvo como objetivo comparar los resultados en la literatura publicada sobre si L-PRF es un adyuvante efectivo y predecible al desbridamiento quirúrgico del hueso necrótico para mejorar la eficacia curativa en pacientes con ONMRM. Materiales y Métodos: Las bases de datos de PubMed, Scopus, Cochrane, ScienceDirect, LILACS y Web of Science se registraron utilizando los términos DeCS/MeSH predeterminados y los criterios de elegibilidad, y la búsqueda arrojó un total de cinco artículos. Dos estudios fueron retrospectivos, y tres estudios fueron series de casos. Resultado: Diecisiete participantes recibieron una combinación de desbridamiento quirúrgico, membrana L-PRF y antibióticos. Se observó curación completa de heridas en el 70% de los participantes, y la mayoría de ellos se curaron sin ninguna complicación. Conclusión: L-PRF como adyuvante para el desbridamiento quirúrgico del hueso necrótico parece tener una asociación positiva con el resultado de curación en pacientes con ONMRM.


Subject(s)
Humans , Bisphosphonate-Associated Osteonecrosis of the Jaw/therapy , Anti-Bacterial Agents/therapeutic use
18.
Rev. Odontol. Araçatuba (Impr.) ; 45(1): 27-32, jan.-abr. 2024. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1553261

ABSTRACT

Desde o incremento das pesquisas das células-tronco em 1961, por cientistas canadenses, os avanços em estudos, pesquisas e o desenvolvimento de novos tratamentos com esse tipo de recurso se mostram promissores. O uso de células-tronco é uma grande aposta tanto para a medicina quanto para a odontologia regenerativa. Os tratamentos com essa terapia podem oferecer mais qualidade de vida para as pessoas. O potencial dessas células tão especiais se encontra em duas características peculiares: elas são capazes de se multiplicarem e de se diferenciarem em outros tipos de células, como de tecidos, cartilagens e neurônios. É dessa maneira que elas têm um papel fundamental para estudos e tratamentos relacionados à regeneração. O uso de células-tronco na Odontologia torna possível diferentes processos odontológicos que oferecem mais qualidade de vida ao paciente. Isso porque fatores como defeitos genéticos, hábitos nocivos, cáries dentárias e perdas precoces dos dentes contribuem com a perda de dentes ao longo da vida. No início do século XXI, por volta dos anos de 2005, 2006, pesquisadores começaram a publicar em revistas internacionais da área uma nova técnica baseada no uso de célulastronco existentes no osso de sustentação dos dentes e na articulação dento alveolar. Esta técnica, chamada de Revascularização, promove o aparecimento de um novo tecido pulpar sadio, devolvendo ao dente sua vitalidade e higidez(AU)


Since the increase in stem cell research in 1961 by Canadian scientists, advances in studies, research and the development of new treatments with this type of resource have shown promise. The use of stem cells is a big bet for both medicine and regenerative dentistry. Treatments with this therapy can offer more quality of life for people. The potential of these very special cells lies in two peculiar characteristics: they are able to multiply and differentiate into other types of cells, such as tissues, cartilage and neurons. It is in this way that they play a key role for studies and treatments related to regeneration. The use of stem cells in dentistry makes possible different dental processes that offer more quality of life to the patient. That's because factors such as genetic defects, harmful habits, tooth decay, and early tooth loss all contribute to lifelong tooth loss. At the beginning of the twenty-first century, around the years 2005, 2006, researchers began to publish in international journals of the area a new technique based on the use of existing stem cells in the supporting bone of the teeth and in the alveolar tooth joint. This technique, called Revascularization, promotes the appearance of a new healthy pulp tissue, returning to the tooth its vitality and hygiene(AU)


Subject(s)
Dental Pulp , Dentistry , Tooth Loss
19.
Odontol. sanmarquina (Impr.) ; 27(1): e26146, ene.-mar.2024.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1556424

ABSTRACT

Uno de los mejores biomateriales usados en odontología es la fibrina rica en plaquetas (PRF), es un concentrado plaquetario de segunda generación que se obtiene a partir de la centrifugación de sangre autóloga y no necesita aditivos. El presente documento busca determinar la eficacia del uso del PRF como parte de la regeneración de tejidos en procedimientos quirúrgicos odontológicos, tomando como base la literatura publicada en PubMed, Elsevier y Semantic Scholar entre 2018 y 2023, la búsqueda de artículos científicos fue ejecutada empleando las palabras clave platalet rich fibrin, regeneration, dentistry, blood buffy coat. La evidencia científica muestra que el PRF puede ser usado en su forma de membrana, gel, tapón, solo o combinado con otro biomaterial para conseguir propiedades biológicas exclusivas que promueven la regeneración y cicatrización mientras reduce los efectos adversos de los procedimientos quirúrgicos. Un ensayo clínico refirió la curación de alvéolos post exodoncia atraumática, y comprobó que la cicatrización con PRF muestra un índice de curación más alto en comparación con el grupo control. El sustento biológico de su eficacia radica en su capacidad para proliferar células que promueven la angiogénesis, osteogénesis y diferenciación celular, es decir, el reparo de tejidos lesionados. Todo esto nos permite llegar a la conclusión de que el PRF representa una alternativa viable y eficaz en procesos de regeneración de tejidos en procedimientos quirúrgicos odontológicos.


One of the best biomaterials used in dentistry is platelet-rich fibrin (PRF). It is a second-generation platelet concentrate obtained by centrifugation of autologous blood and requires no additives. The aim of this article is to determine the effectiveness of using PRF for tissue regeneration in dental surgery. The methodology used consists of a descriptive search of scientific articles that employ or study PRF as a biomaterial for tissue healing in the dental field and are available on PubMed, Elsevier, and Semantic Scholar. The literature shows that PRF can be used as a membrane, gel, cap form, alone or combined with other biomaterials to achieve unique biological properties that promote regeneration and healing while reducing the adverse effects of surgical procedures. For example, a clinical trial demonstrated healing of post-traumatic alveolar exodontia, proving that healing with PRF had a higher healing rate than in the control group. The biological basis of PRF's efficacy lies in its ability to proliferate cells that promote angiogenesis, osteogenesis, and cellular differentiation, and thus repair damaged tissue. All this leads us to conclude that PRF represents a viable and effective alternative in tissue regeneration processes in dental surgery procedures.

20.
Int. j. odontostomatol. (Print) ; 18(1): 66-70, mar. 2024. ilus, tab
Article in Spanish | LILACS | ID: biblio-1558174

ABSTRACT

Los quistes de los maxilares son las lesiones óseas más comunes en la región maxilofacial. La enucleación de las lesiones y el cierre primario de los defectos, son en conjunto, el tratamiento óptimo hoy en día. En algunas ocasiones el defecto óseo resultante puede ser de un tamaño tan grande que afecta la estabilidad de dientes vecinos, comprometa la indemnidad del hueso o produce un retraso cicatrizal que incluso puede impedir una regeneración ósea completa. Se considera que esta falta de regeneración expone al paciente a riesgos de infección tardía, retraso de los tratamientos rehabilitadores en zonas de alta demanda estética y pérdida de vitalidad dentaria. Para disminuir el riesgo de alteraciones en la regeneración ósea completa de cavidades quísticas, se ha propuesto la posibilidad de que tras la enucleación del quiste se rellenen estos defectos con injertos óseos u otras técnicas de preservación alveolar para favorecer la cicatrización. Teóricamente el uso de estos injertos mejora la calidad y disminuye el tiempo de cicatrización ósea, permitiendo que el paciente recupere rápidamente las funciones habituales del componente dentoalveolar, acortando el periodo de cuidados postoperatorios que restringen la alimentación, los deportes o la rehabilitación oral. El actual trabajo tiene como objetivo realizar una revisión de la literatura respecto a los beneficios del uso de injertos óseos en el tratamiento quirúrgico de los quistes maxilares y presentar un caso clínico con los detalles quirúrgicos de esta técnica.


Jaw cysts are the most common bone lesions in the maxillofacial region. Enucleation of the lesions along with the primary closure of the defects are the optimal treatment nowadays. On some occasions, the resulting bone defect can be so large that it affects the stability of neighboring teeth, compromises the integrity of the bone, or produces a delayed healing that can even prevent complete bone regeneration. It is considered that the lack of regeneration exposes the patient to risk of infection, delay of rehabilitation treatments in areas of high aesthetic demand and loss of dental vitality. To reduce the risk of alterations in complete bone regeneration of cystic cavities, the possibility of filling these defects with bone grafts or other alveolar preservation techniques to promote healing, has been proposed after cyst enucleation. Theoretically, the use of these grafts improves the quality and decreases the bone healing time, allowing the patient to quickly recover the usual functions of the dentoalveolar component, limiting the period of postoperative care that restricts eating, sports or oral rehabilitation. The present work aims to carry out a review of the literature regarding the benefits of the use of bone grafts in the surgical treatment of maxillary cysts and to present a clinical case with the surgical details of this technique.


Subject(s)
Humans , Male , Middle Aged , Bone Cysts/surgery , Mandibular Diseases/surgery , Bone Transplantation , Apicoectomy , Tissue Preservation , Bone and Bones/pathology , Bone Regeneration , Mandibular Diseases/pathology , Odontogenic Cysts/surgery , Radicular Cyst/surgery , Tomography, X-Ray Computed , Platelet-Rich Fibrin
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