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Electron. j. biotechnol ; 52: 67-75, July. 2021. tab, graf, ilus
Article in English | LILACS | ID: biblio-1283594


BACKGROUND: Adipogenesis and fibrogenesis can be considered as a competitive process in muscle, which may affect the intramuscular fat deposition. The CCAAT/enhancer-binding protein beta (C/EBPb) plays an important role in adipogenesis, which is well-characterized in mice, but little known in bovine so far. RESULTS: In this study, real-time qPCR revealed that the level of C/EBPb was increased during the developmental stages of bovine and adipogenesis process of preadipocytes. Overexpression of C/EBPb promoted bovine fibroblast proliferation through mitotic clonal expansion (MCE), a necessary process for initiating adipogenesis, by significantly downregulating levels of p21 and p27 (p < 0.01). Also, the PPARc expression was inhibited during the MCE stage (p < 0.01). 31.28% of transfected fibroblasts adopted lipid-laden adipocyte morphology after 8 d. Real-time qPCR showed that C/EBPb activated the transcription of early stage adipogenesis markers C/EBPa and PPARc. Expression of ACCa, FASN, FABP4 and LPL was also significantly upregulated, while the expression of LEPR was weakened. CONCLUSIONS: It was concluded C/EBPb can convert bovine fibroblasts into adipocytes without hormone induction by initiating the MCE process and promoting adipogenic genes expression, which may provide new insights into the potential functions of C/EBPb in regulating intramuscular fat deposition in beef cattle.

Cattle/metabolism , Adipocytes/metabolism , CCAAT-Enhancer-Binding Protein-beta/metabolism , Fibroblasts/metabolism , Adipose Tissue/metabolism , Clone Cells , Cell Proliferation , Adipogenesis , Real-Time Polymerase Chain Reaction , Mitosis , Muscles
Arq. ciências saúde UNIPAR ; 25(1): 61-77, jan-abr. 2021.
Article in Portuguese | LILACS | ID: biblio-1151421


A obesidade é caracterizada pelo aumento excessivo da gordura corporal e está ligada ao estilo de vida, ao meio ambiente e a genética do indivíduo. O equilíbrio entre ingestão e gasto energético é controlado por mecanismos neurais, hormonais, químicos e genéticos. Estudos sugerem que o gene FTO (Fat mass and obesity associated) atua como regulador primário do acúmulo de gordura corporal, quando associado a SNPs (Single Nucleotide Polymorphism) específicos, predispõe à obesidade. O propósito deste trabalho foi verificar a produção científica, analisar e catalogar os estudos de polimorfismos no gene FTO associados à obesidade e suas comorbidades. A busca por publicações entre 2009 e 2018 foi realizada na base de dados SciELO com a palavra-chave "FTO". Foram encontrados 23 artigos originais dentro dos critérios da pesquisa que correlacionam o FTO à obesidade. O nome do autor principal, país, idioma, ano de publicação, título, objetivo, polimorfismo associado e os resultados dos estudos foram extraídos e organizados para facilitar a tabulação dos dados. Também foram pesquisados os números de citações de cada artigo, utilizando-se a plataforma Google Acadêmico. Embora o Brasil se encontre em primeiro lugar em produção científica para o gene FTO na base de dados prospectada, o número de artigos originais ainda é muito modesto. Assim, os resultados encontrados podem servir de subsídio no delineamento de novas pesquisas sobre os polimorfismos do gene FTO e as causas da obesidade.

Obesity is characterized by the excessive increase in body fat and is correlated to the lifestyle, environment, and also to the genetics of the individual. The balance between energy intake and expenditure is controlled by neural, hormonal, chemical, and genetic mechanisms. Studies suggest that the FTO (fat mass and obesity associated), a gene associated with fat mass, plays a role as a primary regulator of body fat buildup, when associated to specific Single Nucleotide Polymorphisms (SNPs), causing predisposition to obesity. This paper aimed at reviewing, analyzing, and cataloguing the studies on FTO gene polymorphisms associated with obesity and its comorbidities. The search was carried out in SciELO database, checking articles published between 2009 and 2018 using the keyword "FTO". Twenty-three original articles, matching the research criteria, correlating FTO either positively or negatively with obesity, were found. The main author's name, country, language, year of publication, title, objective, associated polymorphism, and the study results were extracted and organized to facilitate data tabulation. The citation numbers for each article were also searched by using the Google Scholar platform. Although Brazil ranks first in scientific production on the FTO gene in the surveyed database, the number of original articles is still very modest. Therefore, the results found in this paper may be used as a basis for the design of new research on the FTO gene polymorphisms and the causes of obesity.

Polymorphism, Single Nucleotide , Genetics , Obesity/genetics , Satiety Response , Energy Intake/genetics , Body Mass Index , Adipose Tissue , Lipid Metabolism/genetics , Nutrigenomics , Fats , Genotype , Life Style , Metabolism/genetics
Article in English | WPRIM | ID: wpr-880615


OBJECTIVES@#To study the gene expression of adipose tissue CD14@*METHODS@#The data of GSE54350 were obtained from the public database of gene expression profiling. The data were pre-processed by Network Analyst, String 11.0, Cytoscape 3.7.1, and other analytical software. The differentially expressed genes were analyzed by gene ontology biological function and kyoto encycopedia of genes and genomes (KEGG) signaling pathway to establish differential gene protein interaction network, transcription factor-gene regulatory network, microRNA-gene regulatory network, environmental factors-gene regulatory network, and other interaction systems.@*RESULTS@#The gene expression pattern of CD14@*CONCLUSIONS@#The gene expression of adipose tissue CD14

Adipose Tissue , Computational Biology , DNA-Binding Proteins , Diabetes Mellitus, Type 2/genetics , Gene Expression , Gene Expression Profiling , Gene Regulatory Networks , Humans , MicroRNAs/genetics , Muscle Proteins
Article in Chinese | WPRIM | ID: wpr-880072


The normal hematopoiesis of the body are depends on the proliferation and differentiation of hematopoietic stem/progenitor cell (HSPC), as well as the mesenchymal stem/stromal cell (MSC) that support the growth and development of hematopoietic microenvironment of bone marrow (BM). At present, the commonly used MSC for promoting hematopoiesis are mainly derived from BM, however, the acquisition of MSC from BM is limited by the number, sampling, isolation and purification. Compared with BM, adipose tissue has many advantages, including widely distributed, abundant in source, simple and easy to obtain, and contains more pluripotent stem cell, such as adipose tissue-derived stem cell (ADSC), in which Perivascular cell/pericyte (PC) are considered as the precursor cell of MSC, also is the main components of vascular microenvironment, and plays an important role in the proliferation and differentiation of HSPC. PC is especially abundant in adipose tissue, and with the advantages of easy acquisition, small damage, fast cell proliferation and low immunogenicity. Therefore, the sustaining hematopoiess of human adipose derived-perivascular stem cell (AD-PC) to HSPC requires further research and exploration. In this review, the possible supporting effects and PC subgroup of ADSC as stromal cell on HSPC are summarized briefly.

Adipose Tissue , Bone Marrow Cells , Cell Differentiation , Cell Proliferation , Cells, Cultured , Hematopoietic Stem Cell Transplantation , Hematopoietic Stem Cells , Humans , Mesenchymal Stem Cells
Article in Chinese | WPRIM | ID: wpr-878987


To investigate the effect of Gegen Qinlian Decoction(GQD) on enzyme activity, gene expression and methylation level of fatty acid synthase(FASN) in adipose tissue from rats with insulin resistance induced by high-fat diet. The 60% fat-powered high-fat diet was continuously given to male SD rats to induce the insulin resistance model. Then, they were divided into five groups randomly and administrated by gavage every day for 16 weeks with following drugs respectively: 10 mL·kg~(-1)water for control group(C) and insulin resistance model control group(IR), 1.65 g·kg~(-1)GQD per day for low-dose group(GQDL), 4.95 g·kg~(-1)GQD per day for medium-dose group(GQDM), 14.85 g·kg~(-1)GQD per day for high-dose group(GQDH), and 5 mg·kg~(-1) rosiglitazone per day for rosiglitazone group(RGN). Epididymal adipose tissue was taken to determine enzyme activity of FASN by colorimetric method, mRNA expression level of Fasn by quantitative Real-time PCR(Q-PCR) and CpGs methylation level between +313 and +582 by bisulfite sequencing PCR(BSP). These results showed that Fasn expression was significantly lowered in IR model rats compared with the control rats(P<0.01). Enzymatic activity and CpGs methylation level of Fasn in IR group showed downward trends. Low and medium-dose GQD can increase enzyme activity of FASN(P<0.05). Moreover, low-dose GQD increased the total CpGs methylation level of Fasn fragment between +313 and +582 in insulin resistance rats(P<0.05). For GQDM group, the methylation frequency of CpGs at positions +506 and +508(P<0.01) as well as the methylation frequency of CpGs on the binding sites of transcription factorzinc finger protein 161(P<0.05) were significantly increased. The methylation frequency of CpG at +442 position was positively correlated with Fasn expression(P<0.01, r=0.735), and methylation frequencies of CpGs at +345 and +366 positions were positively associated to enzyme activity of FASN respectively(P<0.05, r=0.479; P<0.01, r=0.640). In conclusion, GQD can reverse enzyme activity of FASN and methylation level of Fasn in adipose tissue of insulin resistant rats, and CpG sites at positions +506 and +508 may be the targets of GQD. The methylation level of CpGs at + 345 and + 366 sites were possibly related to FASN activity, while methylation of CpG at + 442 site may be closely correlated with mRNA level of Fasn. In addition, GQD did not significantly change mRNA expression level of Fasn, but effectively reversed enzymatic activity, suggesting that GQD may regulate the post transcriptional expression of Fasn.

Adipose Tissue , Animals , Drugs, Chinese Herbal , Fatty Acid Synthases/genetics , Gene Expression , Insulin Resistance/genetics , Male , Methylation , Rats , Rats, Sprague-Dawley
Article in Chinese | WPRIM | ID: wpr-878709


Adipokines,the bioactive polypeptides secreted by adipose tissue,are related to the occurrence and development of obesity,metabolic syndrome,renal insufficiency,cardiovascular disease,diabetes mellitus and other diseases.They may be the disease intervention targets and a breakthrough in the study of disease pathogenesis.In this paper,we summarize the latest research progress of the adipokines omentin,chemerin and nesfatin.

Adipokines , Adipose Tissue , Chemokines , Cytokines , Humans , Kidney Diseases , Metabolic Syndrome , Obesity
Acta Physiologica Sinica ; (6): 115-125, 2021.
Article in Chinese | WPRIM | ID: wpr-878241


In eukaryotic cells, the endoplasmic reticulum (ER) is the key quality control organelle for cellular protein synthesis and processing. It also serves as an important site for Ca

Adipose Tissue , Diabetes Mellitus, Type 2 , Endoplasmic Reticulum Stress , Endoribonucleases , Humans , Protein-Serine-Threonine Kinases , eIF-2 Kinase
Chinese Medical Journal ; (24): 716-724, 2021.
Article in English | WPRIM | ID: wpr-878011


BACKGROUND@#Obesity is a fundamental factor in metabolic disorders such as hyperlipidemia, insulin resistance, fatty liver, and atherosclerosis. However, effective preventive measures are still lacking. This study aimed to investigate different surgical protocols for removing partial adipose tissue before the onset of obesity and determine whether, and by which protocol, preliminary adipose removal could exert potent preventive effects against diet-induced metabolic disorders.@*METHODS@#Male low-density lipoprotein receptor (LDL-R) knockout (KO) mice were randomly divided into four groups and subjected to epididymal fat removal (Epi-FR) surgery, subcutaneous fat removal (suQ-FR) surgery, both subcutaneous and epididymal fat removal (Epi + suQ-FR) surgery, or sham-operation. After 1 week of recovery, all mice were given a high-fat diet (HFD) for 10 weeks to induce metabolic disorders.@*RESULTS@#In the Epi-FR group and the sham-operated group, the mean numbers of the residual subcutaneous fat were 28.59 mg/g and 18.56 mg/g, respectively. The expression of relative genes such as Pparg, Cebpa, Dgat2, Fabp4 and Cd36 in the residual subcutaneous fat increased 2.62, 3.90, 3.11, 2.06, 1.78 times in the Epi-FR group compared with that in the sham-operated group. Whereas in the other fat-removal groups, the residual fat depots had no significant change in either size or gene expression, as compared with those of the sham-operated group. Plasma lipid and glucose levels and insulin sensitivity, as detected by the glucose tolerance test, were not significantly alleviated in the three fat removal groups. Liver mass or lipid content was not attenuated in any of the three fat removal groups. The atherosclerosis burdens in the entire inner aorta and aortic root did not decrease in any of the three fat removal groups.@*CONCLUSIONS@#Our data suggest that removal of epididymal adipose or subcutaneous adipose alone or in combination before the onset of obesity did not protect against hyperlipidemia, insulin resistance, fatty liver, or atherosclerosis in LDL-R KO mice fed with a HFD. Hence, adipose removal possibly does not represent a potential approach in preventing obesity-related metabolic disorders in the obesity-susceptible population.

Adipose Tissue , Animals , Diet, High-Fat/adverse effects , Insulin Resistance , Liver , Male , Mice , Mice, Inbred C57BL , Obesity , Subcutaneous Fat
Article in Chinese | WPRIM | ID: wpr-877566


OBJECTIVE@#To observe the effect of acupoint thread-embedding at "Zusanli" (ST 36) and "Fenglong" (ST 40) on the macrophage polarization of epididymis adipose tissue in obese mice, and to explore the action mechanism of acupoint thread-embedding on weight control.@*METHODS@#Among 30 male C57BL/6 mice, 10 mice were randomly selected and fed with normal diet, and the remaining 20 mice were fed with high-fat diet to establish the obesity model. Sixteen mice with successful obesity model were randomly divided into a model group and an acupoint thread-embedding group, 8 mice in each group. Eight mice were selected from mice which were fed with normal diet as the normal group. On the next day of successful modeling, acupoint thread-embedding was performed at "Zusanli" (ST 36) and "Fenglong" (ST 40) in the acupoint thread-embedding group, once every 10 days for 4 times. The body weight was recorded at 0, 8, 16, 24, 32, 40 days into intervention; the level of glucose metabolism was compared after intervention; the level of lipid metabolism and weight of epididymal adipose tissue were compared at the end of the intervention; the mRNA expression of M1 and M2 macrophage-related cytokines interleukin-10 (IL-6), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were detected by real-time PCR; the mRNA and protein expression of M1 macrophage labeled inducible nitric oxide synthase (iNOS) and M2 macrophage labeled arginase-1 (Arg-1) were detected by real-time PCR and Western blot.@*RESULTS@#Compared with the normal group, the body weight at 0, 8, 16, 24, 32, 40 days into intervention in the model group was increased (@*CONCLUSION@#Acupoint thread-embedding at "Zusanli" (ST 36) and "Fenglong" (ST 40) may play a role in weight control by regulating the polarization of macrophages.

Acupuncture Points , Adipose Tissue , Animals , Epididymis , Macrophages , Male , Mice , Mice, Inbred C57BL , Mice, Obese
Braz. j. med. biol. res ; 54(2): e9944, 2021. tab, graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1142581


The aim of this study was to inhibit adipogenic differentiation by transfecting two growth factors, platelet-derived growth factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2), into modified rat bone marrow mesenchymal stem cells (rBMSCs) and then compounded with platelet-rich plasma (PRP). To achieve rBMSCs, the osteoporosis model of rats was established, and then the rBMSCs from the rats were isolated and identified. Co-transfection of rBMSCs with PDGF-BB-GFP and BMP-2 and detection of PDGF-BB/BMP-2 expression in transfected BMSCs was assessed by qRT-PCR and western blot, respectively. Moreover, the effect of the two growth factors transfection of rBMSCs on adipogenic differentiation was evaluated by oil red O staining and western blot, respectively. Finally, construction of the two growth factors transfection of rBMSCs compounded with PRP and detection of adipogenic differentiation were assessed by oil red O staining, CCK-8, and western blot, respectively. In vitro studies revealed that the two growth factors transfection of rBMSCs compounded with PRP promoted cell viability and inhibited adipogenic differentiation and could be promising for inhibiting adipogenic differentiation.

Animals , Rats , Cell Differentiation , Adipose Tissue/cytology , Platelet-Rich Plasma , Bone Morphogenetic Protein 2/genetics , Mesenchymal Stem Cells/cytology , Becaplermin/genetics , Transfection , Cells, Cultured
Rev. Odontol. Araçatuba (Impr.) ; 41(3): 15-21, set./dez. 2020. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1121724


Introdução: A bichectomia é um procedimento cirúrgico que remove parte do corpo adiposo bucal não apenas para fins estéticos, mas também funcionais, limitando o trauma recorrente na região jugal. A correta indicação cirúrgica e o auxílio de exames de imagem são essenciais para o planejamento cirúrgico e seleção da técnica a ser utilizada, minimizando as taxas de complicações e alcançando resultados seguros e satisfatórios. Objetivo: Relatar dois casos clínicos em que a bichectomia foi realizada, enfatizando a importância da ultrassonografia pré-operatória na determinação do volume do corpo adiposo bucal e abordando duas técnicas cirúrgicas. Relato de casos clínicos: Duas pacientes do sexo feminino (18 e 32 anos), normosistêmicas, relataram queixas de insatisfação estética com o contorno arredondado da face, além de trauma constante na mucosa jugal. Ao exame clínico, as pacientes apresentavam mucosa jugal de volume aumentado e traumatizado. A bichectomia foi realizada pelo acesso de Matarasso e pelo fundo de sulco. Conclusão: a ultrassonografia mostrou-se eficiente no planejamento cirúrgico da bichectomia, favorecendo a retirada do corpo adiposo da bochecha de forma previsível, rápida e segura. As duas técnicas cirúrgicas foram eficazes para remoção do corpo adiposo bucal(AU)

Introduction: Bichectomy is a surgical procedure that removes part of the buccal fat pad not only for esthetic but also functional purposes, limiting recurrent trauma in the jugal region. The correct surgical indication and the aid of imaging examinations are essential for the surgical planning and selection of the technique to be used, minimizing complication rates and achieving safe and satisfactory results. Objective: To report two clinical cases in which bichectomy was performed, emphasizing the importance of preoperative ultrasonography in determining the volume of the buccal fat pad. Report of clinical cases: Two female patients (18 and 32 years), normosistemic, reported complaints of aesthetic dissatisfaction with the rounded contour of the face, in addition to a constant complaint of trauma to the jugal mucosa. At the clinical examination, the patients presented jugal mucosa of increased and traumatized volume. A bichectomy was performed by Matarasso access and by the furrow fund. Conclusion: ultrasonography proved to be efficient in assisting the surgical planning of bichectomy, favoring the removal of the buccal fat pad in a predictable, fast and safe way. The two surgical techniques were effective for removal of the buccal fat pad(AU)

Cheek , Cheek/surgery , Surgery, Oral , Adipose Tissue , Ultrasonography , Esthetics , Face
Pesqui. vet. bras ; 40(12): 1018-1028, Dec. 2020. tab, graf, ilus
Article in English | ID: biblio-1155043


The present study aimed to evaluate the effects of mesenchymal stem cells derived from canine adipose tissue in the healing process of full-thickness mesh skin grafts in rabbits. The stem cells were collected from young dogs; and, after characterization, remained in cryopreservation, in independent doses containing 2 x 106 cells. The mesh distal limb graft technique was performed in 60 rabbits, divided into three groups, CG (Control Group), GT1 (Intralesional Stem Cell Treated Group), and GT2 (Intravenous Stem Cell Treated Group), containing 20 animals each. After grafting, each group was randomly divided into four subgroups according to euthanasia time 3, 7, 14, and 30 days, containing five animals in each group. Animals of GT1_14, GT1_30, and GT2_14, GT2_30 subgroups received a second dose of xenogeneic cells on the seventh day. Meanwhile, animals from GT1_30 and GT2_30 received the third dose of xenogeneic cells on day 14. The groups treated with xenogeneic stem cells positively affected type III collagen re-epithelialization and deposition, and possibly GT1 had a controlled inflammatory response. However, no effect on angiogenesis. Thus, it was possible to demonstrate tolerance and therapeutic action of mesenchymal stem cells from canine adipose tissue in skin grafts in rabbits.(AU)

O presente estudo teve como principal objetivo avaliar os efeitos das células-tronco mesenquimais derivadas do tecido adiposo de cães no processo de cicatrização de autoenxertos de pele de espessura total em malha em coelhos. As células-tronco foram coletadas de cães jovens, após a caracterização estas permaneceram em criopreservação, em doses individuais contendo 2 x 106 células. A técnica de enxerto em malha na região distal do membro foi realizada em 60 coelhos, divididos em três grupos, GC (Grupo Controle), GT1 (Grupo tratado com células-tronco intralesional) e GT2 (Grupo tratado com células-tronco via endovenosa), contendo 20 animais cada. Imediatamente após a enxertia, cada grupo foi dividido aleatoriamente em quatro subgrupos, de acordo com o tempo de eutanásia 3, 7, 14 e 30 dias contendo cinco animais cada. Animais dos subgrupos GT1_14, GT1_30 e GT2_14, GT2_30 receberam uma segunda dose de células xenógenas no sétimo dia. Ademais, animais do GT1_30 e do GT2_30 receberam a terceira dose de células xenógenas no dia 14. Os grupos tratados com células-tronco xenógenas tiveram um efeito positivo na reepitelização e deposição de colágeno tipo III, e possivelmente, o GT1 teve uma resposta inflamatória controlada, entretanto o efeito na angiogênese não foi observado. Dessa forma, foi possível demonstrar que houve tolerância e ação terapêutica das células-tronco mesenquimais derivadas do tecido adiposo de cães em enxertos de pele em coelhos.(AU)

Animals , Dogs , Rabbits , Stem Cells , Adipose Tissue , Transplants , Mesenchymal Stem Cells , Autografts , Wound Healing , Neovascularization, Physiologic
Rev. bras. cir. cardiovasc ; 35(6): 964-969, Nov.-Dec. 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1143982


Abstract Perivascular adipose tissue (PVAT) is a source of factors affecting vasomotor tone with the potential to play a role in the performance of saphenous vein (SV) bypass grafts. As these factors have been described as having constrictor or relaxant effects, they may be considered either beneficial or detrimental. The close proximity of PVAT to the adventitia provides an environment whereby adipose tissue-derived factors may affect the vasa vasorum, a microvascular network providing the vessel wall with oxygen and nutrients. Since medial ischaemia promotes aspects of graft occlusion the involvement of the PVAT/vasa vasorum axis in vein graft patency should be considered.

Saphenous Vein , Vasa Vasorum , Adipose Tissue , Femoral Vein
Electron. j. biotechnol ; 48: 72-77, nov. 2020. tab, ilus
Article in English | LILACS | ID: biblio-1254810


BACKGROUND: To identify differentially expressed genes (DEGs) between muscle and adipose in cattle, we analyzed the data from the RNA sequencing of three Angus×Qinchuan crossbred cattle. RESULTS: Searched the Gene Expression Omnibus (GEO) for a microarray dataset of Yan yellow cattle, GSE49992. After the DEGs were identified, we used STRING and Cytoscape to construct a protein­protein interaction (PPI) network, subsequently analyzing the major modules of key genes. In total, 340 DEGs were discovered, including 21 hub genes, which were mainly enriched in muscle contraction, skeletal muscle contraction, troponin complex, lipid particle, Z disc, tropomyosin binding, and actin filament binding. CONCLUSIONS: In summary, these genes can be regarded as candidate biomarkers for the regulation of muscle and adipose development.

Animals , Cattle , Adipose Tissue/growth & development , Muscle Development/genetics , Transcriptome/genetics , Gene Expression , Gene Expression Regulation, Developmental , Computational Biology , RNA-Seq
Arch. argent. pediatr ; 118(5): 320-326, oct 2020. ilus, tab
Article in English, Spanish | LILACS, BINACIS | ID: biblio-1122019


Objetivo. Analizar la asociación entre la fuerza de prensión manual relativa (FPMR) y la masa grasa (MG), controlando el posible efecto del estado de madurez.Metodología. Se estudiaron niños y adolescentes de ambos sexos con un rango de edad de ≥ 7,5 a ≤ 15,49 años. Se evaluó la FPMR mediante un dinamómetro hidráulico manual. El porcentaje de MG se evaluó mediante la absorciometría de rayos X de doble energía. Para analizar las diferencias por sexo y grupo etario y entre los niños normopesos y obesos en los niveles de FPMR, se utilizó el análisis de covarianza. Se consideraron como covariable los años de pico de velocidad de crecimiento (estado de madurez). Para analizar la asociación entre los niveles de FPMR y MG, se utilizó la correlación parcial, controlando el año de pico de velocidad de crecimiento.Resultados. Participaron 1685 escolares (731 mujeres y 954 varones). Fueron conformados 4 grupos de edad (7,5-9,4 años; 9,5-11,4 años; 11,5-13,4 años y 13,5-15,4 años). La FPMR aumentó con la edad en ambos sexos. Los valores de MG fueron elevados en todos los grupos etarios. No hubo diferencias en los grupos 3 y 4 en las niñas y en el grupo 4 de los niños. Los niños clasificados como peso normal presentaron significativamente mayor FPMR que los obesos. Se observó una asociación negativa entre FPMR y MG.Conclusión. Se demostró que la FPMR estaba negativamente asociada con la MG, tras controlar el efecto del estado de madurez

Objective. To analyze the association between relative handgrip strength (RHGS) and fat mass (FM) after controlling for the potential effect of maturity status.Methodology. Both male and female children and adolescents aged ≥ 7.5 to ≤ 15.49 years were studied. RHGS was assessed using a hydraulic hand dynamometer. FM percentage was established using a dual-energy X-ray absorptiometry. Age and sex differences in RHGS levels between normal weight and obese participants were studied with an analysis of covariance. A covariate was years from peak height velocity (maturity status). The association between RHGS and FM levels was analyzed using a partial correlation and controlling for age at peak height velocity.Results. A total of 1685 students (731 girls and 954 boys) participated. Four age groups were established (7.5-9.4 years, 9.5-11.4 years, 11.5-13.4 years, and 13.5-15.4 years). RHGS increased with age in both males and females. FM values were high in all age groups. No differences were observed in groups 3 and 4 among girls or in group 4 among boys. Participants classified as normal weight showed a significantly higher RHGS than their obese peers. A negative association was noted between RHGS and FM.Conclusion. RHGS was shown to be negatively associated with FM after controlling for the effect of maturity status

Humans , Male , Female , Child , Adolescent , Muscle Strength , Body Mass Index , Chile , Adipose Tissue , Epidemiology, Descriptive , Growth and Development , Obesity
Fisioter. Bras ; 21(4): 396-406, Ago 08, 2020.
Article in Portuguese | LILACS | ID: biblio-1283410


Introdução: A adiposidade localizada é considerada uma das disfunções estéticas mais procuradas para tratamento no âmbito da estética. Como opção de tratamento apresentamos a lipoescultura gessada que é um método não invasivo e inovador, que consiste em reduzir a lipodistrofia localizada pelo aumento do metabolismo através da Lei de Van't Hoff. Consta em tratamento baseado em uma linha de cosmecêuticos ortomoleculares da marca Bothanica Mineral®, empregados no combate à celulite, gordura localizada e flacidez, agindo de forma diversificada nas disfunções estéticas. Objetivo: O objetivo deste trabalho foi identificar os efeitos da lipoescultura gessada no abdome em mulheres de 20 a 35 anos com IMC ideal. Métodos: Trata-se de uma pesquisa intervencional. O estudo foi composto por 36 mulheres, apresentando lipodistrofia localizada no abdome, selecionadas através de entrevista. Após seleção, foram coletados dados através da ficha de avaliação da lipoescultura gessada contendo perfil sociodemográfico, bioimpedância, perimetria abdominal e caracterização da gordura, com um total de 8 sessões, 2 vezes na semana. Foi realizada uma reavaliação delas na nona sessão. Resultados: Diversos são os tipos de aparelhos e procedimentos estéticos disponíveis para intensificar os resultados do tratamento da lipodistrofia localizada do abdome em mulheres, porém, uma lipoescultura gessada bem-feita e associada a bons cosméticos e cuidados diários após o tratamento permitem um ótimo resultado, como comprovado em nosso trabalho. Conclusão: Conclui-se, então, que o protocolo de tratamento da lipoescultura gessada resultou em melhorias das condições gerais de gordura das mulheres estudadas, melhorando o contorno corporal. (AU)

Introduction: Localized adiposity is considered one of the most sought aesthetic dysfunctions for aesthetic treatment. As a treatment option we present a noninvasive and innovative method, the plastered liposculpture, which consists in reducing localized lipodystrophy by increasing metabolism through Van't Hoff's Law. It is based on a line of Orthomolecular cosmeceuticals Bothanica Mineral®, used in the therapy against cellulite, localized fat and flaccidity, acting in a diversified way in aesthetic dysfunctions. Objective: The objective of this study was to identify the effects of plastered liposculpture on the abdomen in women aged 20 to 35 years with ideal BMI. Methods: This is an interventional research. The study was composed of 36 women, presenting lipodystrophy located in the abdomen, selected through interview. After selection, data were collected through the plastered liposculpture evaluation card containing sociodemographic profile, bioimpedance, abdominal perimetry and fat characterization, with 8 sessions, 2 times a week. A reassessment was made at the ninth session. Results: Several types of devices and aesthetic procedures are available to intensify the results of the treatment of localized lipodystrophy of the abdomen in women, however, a well-made plastered liposculpture, associated with good cosmetics and daily care after treatment allow a significative result, such as proven in our work. Conclusion: It was concluded that the treatment protocol of plastered liposculpture resulted in improvement in the general fat conditions of the studied women, improving the body contour. (AU)

Humans , Female , Adult , Adipose Tissue , Abdomen , Fats , Esthetics
Rev. bras. cir. cardiovasc ; 35(4): 471-476, July-Aug. 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1137315


Abstract Objective: To examine the relationship between the left atrial (LA) thrombus presence and the epicardial adipose tissue (EAT) thickness. Methods: Three hundred and twelve consecutive rheumatic mitral valve stenosis (RMVS) patients with mitral valve area (MVA) < 2 cm2 were included in this cross-sectional study. Patients were divided into two groups, those with and those without LA thrombus. Routine biochemical analysis and electrocardiographic examinations were carried out. EAT was measured using transthoracic echocardiography. Results: LA thrombus was determined in 84 (26.9%) RMVS patients. In echocardiographic examinations, higher mean gradient and LA diameter as well as lower MVA were found in the group with LA thrombus (P<0.001). In this group, higher C-reactive protein (CRP) and EAT values were also determined (P<0.001). There was significant correlation between EAT and MVA, CRP, LA appendage peak flow velocity, LA anteroposterior diameter, and mean gradient (P<0.001). Higher EAT values were identified as independently associated with the presence of LA thrombus (odds ratio 59.5; 95% confidence interval 12.1-290.10; P<0.001). Conclusion: Transthoracic echocardiography, routinely used in patients with RMVS, can measure EAT to determine patients who are under risk for thrombus.

Humans , Male , Female , Adult , Middle Aged , Aged , Thrombosis/complications , Thrombosis/diagnostic imaging , Mitral Valve Stenosis/etiology , Mitral Valve Stenosis/diagnostic imaging , Adipose Tissue/diagnostic imaging , Cross-Sectional Studies , Echocardiography, Transesophageal , Heart Atria/diagnostic imaging
Arq. ciências saúde UNIPAR ; 24(2): 81-85, maio-ago. 2020.
Article in Portuguese | LILACS | ID: biblio-1116356


A obesidade possui vários prejuízos para a saúde e está associada à inúmeras patologias e baixa expectativa de vida. O desequilíbrio alimentar é um fator que necessita de atenção especial, pois é capaz de alterar as interações entre nutrientes e genes. O objetivo deste trabalho foi verificar as principais linhas de pesquisa associadas à nutrigenômica, e evidenciar a relação da influência da nutrição na expressão de genes relacionados à obesidade. Realizou-se o levantamento bibliográfico e a análise cienciométrica por meio do banco de dados publicados na Biblioteca Virtual em Saúde (BVS) e do Centro Latino-Americano de Informação em Ciências da Saúde (BIREME). Identificou-se 118 artigos originais, os quais foram agrupados em cinco classes: restrição calórica, expressão gênica, alimentos, intervenção dietética e diversos. Os resultados evidenciaram que a restrição calórica possui relação direta da expressão gênica com o controle das células cancerígenas e a diminuição do excesso de tecido adiposo. Além disso, a análise cienciométrica relacionou a importância das fibras alimentares na redução do colesterol e sensibilidade à insulina, bem como a ação do jejum na regulação negativa de genes que contribuem para o crescimento do tecido adiposo. Dessa forma, este artigo fornece princípios ideológicos para auxiliar especialistas na aplicabilidade de estratégias para atingir a redução de peso sustentável por meio da expressão gênica.

Obesity has several health risks and is associated with numerous pathologies and low life expectancy. Food imbalance is a factor that needs special attention, as it is able to change the interactions between nutrients and genes. This study aimed at verifying the main lines of research associated with nutrigenomics, and at showing the relationship of the influence of nutrition on the expression of genes related to obesity. The bibliographic survey and scientometric analysis were carried out through the database published in the Biblioteca Virtual em Saúde (BVS) and the Centro Latino-Americano de Informação em Ciências da Saúde (BIREME). A total of 118 articles of original research were identified, and were grouped into five categories: caloric restriction; gene expression; food; dietary intervention; and miscellaneous. The results showed that caloric restriction has a direct relationship between gene expression and the control of cancer cells and the reduction of excess adipose tissue. Furthermore, the scientometric analysis related the importance of dietary fibers in reducing cholesterol and insulin sensitivity as well as the action of fasting in the negative regulation of genes that contribute to the growth of adipose tissue. Thus, this paper provides ideological principles to assist specialists in the applicability of strategies to achieve sustainable weight reduction through gene expression.

Gene Expression/genetics , Food/adverse effects , Obesity/genetics , Dietary Fiber , Weight Loss , Adipose Tissue , Caloric Restriction , Dietetics , Nutritional Sciences , Nutrigenomics , Insulin , Neoplasms
Electron. j. biotechnol ; 46: 30-37, jul. 2020. tab, graf
Article in English | LILACS | ID: biblio-1223233


BACKGROUND: The effects of dietary nutrition on tail fat deposition and the correlation between production performance and the Hh signaling pathway and OXCT1 were investigated in fat-tailed sheep. Tan sheep were fed different nutritional diets and the variances in tail length, width, thickness and tail weight as well as the mRNA expression of fat-related genes (C/EBPα, FAS, LPL, and HSL) were determined in the tail fat of sheep at three different growth stages based on their body weight. Furthermore, the correlations between tail phenotypes and the Hedgehog (Hh) signaling pathway components (IHH, PTCH1, SMO, and GLI1) and OXCT1 were investigated. RESULTS: C/EBPα, FAS, LPL, and HSL were expressed with differences in tail fat of sheep fed different nutritional diets at three different growth stages. The results of the two-way ANOVA showed the significant effect of nutrition, stage, and interaction on gene expression, except the between C/EBPα and growth stage. C/EBPα, FAS, and LPL were considerably correlated with the tail phenotypes. Furthermore, the results of the correlation analysis demonstrated a close relationship between the tail phenotypes and Hh signaling pathway and OXCT1. CONCLUSIONS: The present study demonstrated the gene-level role of dietary nutrition in promoting tail fat deposition and related tail fat-related genes. It provides a molecular basis by which nutritional balance and tail fat formation can be investigated and additional genes can be identified. The findings of the present study may help improve the production efficiency of fat-tailed sheep and identify crucial genes associated with tail fat deposition.

Animals , Tail/metabolism , Sheep/genetics , Adipose Tissue , Diet , Phenotype , RNA, Messenger , Coenzyme A-Transferases , Gene Expression , Body Fat Distribution , Adipogenesis , Lipogenesis/genetics , Hedgehog Proteins/genetics , Real-Time Polymerase Chain Reaction